Name: Bosea robiniae R-46070
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1793

Type strain:

Species: Bosea robiniae

Strain Designation: R-46070

Culture col. no.: DSM 26672, CCUG 61249, LMG 26381

Strain history: <- LMG <- A. Willems and S. De Meyer, Group Biochemistry and Microbiology, University of Ghent

NCBI tax ID(s): 1036780 (species)

SI-ID 371082

LMG 26381, CCUG 61249, DSM 26672

Section

Bosea robiniae R-46070 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from root nodules of Robinia pseudoacacia.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Boseaceae*
	Genus	*Bosea*
	Species	**Bosea robiniae**
	Full Scientific Name (LPSN)	Bosea robiniae De Meyer and Willems 2012

Information on morphological and physiological properties Morphology

[Ref.: #23325]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.991

[Ref.: #23325]	Cell length	1.1-1.4 µm

[Ref.: #23325]	Cell width	0.8 µm

[Ref.: #23325]	Cell shape	rod-shaped

[Ref.: #23325]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %97.495

Information on culture and growth conditions Culture and growth conditions

[Ref.: #23325]

Culture medium

YMA

[Ref.: #23325]

Culture medium growth

[Ref.: #18269]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #18269]

Culture medium growth

[Ref.: #18269]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18269]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #18269]		growth	28 °C
[Ref.: #23325]		growth	25 °C
[Ref.: #23325]		growth	28 °C
[Ref.: #23325]		growth	33 °C
[Ref.: #23325]	no	growth	42 °C
[Ref.: #62590]		growth	30 °C

[Ref.: #18269]	Temperature range	mesophilic
[Ref.: #23325]	Temperature range	mesophilic
[Ref.: #23325]	Temperature range	thermophilic
[Ref.: #62590]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #62590]

Oxygen tolerance

aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23325]	adipate ChEBI	-	assimilation
[Ref.: #23325]	citrate ChEBI	-	assimilation
[Ref.: #23325]	D-glucose ChEBI	-	assimilation
[Ref.: #23325]	D-mannitol ChEBI	-	assimilation
[Ref.: #23325]	D-mannose ChEBI	-	assimilation
[Ref.: #23325]	L-arabinose ChEBI	-	assimilation
[Ref.: #23325]	malate ChEBI	-	assimilation
[Ref.: #23325]	maltose ChEBI	-	assimilation
[Ref.: #23325]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #23325]	nitrate ChEBI	+	reduction
[Ref.: #23325]	phenylacetate ChEBI	+	assimilation
[Ref.: #23325]	potassium gluconate ChEBI	+/-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #23325]	amoxicillin ChEBI		yes
[Ref.: #23325]	doxycycline ChEBI	yes
[Ref.: #23325]	penicillin ChEBI		yes

	Enzyme	Enzyme activity	EC number
[Ref.: #23325]	beta-glucosidase	+	3.2.1.21
[Ref.: #23325]	catalase	+	1.11.1.6
[Ref.: #23325]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23325]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #18269]	Sample type/isolated from	root nodules of Robinia pseudoacacia
[Ref.: #18269]	Host species	Robinia pseudoacacia
[Ref.: #18269]	Geographic location (country and/or sea, region)	Flanders, Aalst, industrial area
[Ref.: #18269]	Country	Belgium
[Ref.: #18269]	Country ISO 3 Code	BEL
[Ref.: #18269]	Continent	Europe

[Ref.: #62590]	Sample type/isolated from	Root nodule,Robinia pseudoacacia
[Ref.: #62590]	Sampling date	2008-09-04
[Ref.: #62590]	Geographic location (country and/or sea, region)	Flanders,Aalst
[Ref.: #62590]	Country	Belgium
[Ref.: #62590]	Country ISO 3 Code	BEL
[Ref.: #62590]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FR774994 (>99% sequence identity) for Bosea from Microbeatlas

Aquatic Samples	64
Soil Samples	94
Animal Samples	131
Plant Samples	207
Total Samples	496

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18269]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #18269]	Bosea sp. R-46070 partial 16S rRNA gene, strain R-46070	FR774994	1439	ENA	1036780 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bosea robiniae DSM 26672	GCA_900102525	contig	GenBank	1036780 tax ID	*
[Ref.: #66792]	Bosea robiniae strain DSM 26672	1036780.5	wgs	PATRIC	1036780 tax ID	*
[Ref.: #66792]	Bosea robiniae DSM 26672	2675903146	draft	JGI IMG/M	1036780 tax ID	*

[Ref.: #23325]

GC-content

65.0 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bosea robiniae strain DSM 26672 PATRIC: 1036780.5
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bosea robiniae strain DSM 26672 PATRIC: 1036780.5
[Ref.: #69480]	motile	yes	91.474	yes
[Ref.: #69480]	flagellated	no	60.871	no
[Ref.: #69480]	gram-positive	no	98.384	yes
[Ref.: #69480]	anaerobic	no	98.027	yes
[Ref.: #69480]	aerobic	yes	94.523	yes
[Ref.: #69480]	halophile	no	93.447	no
[Ref.: #69480]	spore-forming	no	96.33	no
[Ref.: #69480]	thermophile	no	97.227	no
[Ref.: #69480]	glucose-util	yes	85.47	yes
[Ref.: #69480]	glucose-ferment	no	90.396	no

Availability in culture collections External links

[Ref.: #18269]

Culture collection no.

DSM 26672, CCUG 61249, LMG 26381

[Ref.: #71440]

SI-ID 371082

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumes.	De Meyer SE, Willems A	Int J Syst Evol Microbiol	10.1099/ijs.0.035477-0	2011	*

References

#18269

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26672

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23325

Sofie E. De Meyer, Anne Willems: Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumes. IJSEM 62: 2505 - 2510 2012 ( DOI 10.1099/ijs.0.035477-0 , PubMed 22155761 )

#62590 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 61249

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71440

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID371082.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Bosea robiniae in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Bosea robiniae strain DSM 26672

Bosea robiniae strain DSM 26672

Change proposal

Change proposal