Name: Bosea lupini R-45681
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1792

Type strain:

Species: Bosea lupini

Strain Designation: R-45681

Culture col. no.: DSM 26673, CCUG 61248, LMG 26383

Strain history: <- LMG <- A. Willems and S. De Meyer, Group Biochemistry and Microbiology, University of Ghent

NCBI tax ID(s): 1036779 (species)

SI-ID 371081

LMG 26383, CCUG 61248, DSM 26673

Section

Bosea lupini R-45681 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from root nodules of Lupinus polyphyllus.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Boseaceae*
	Genus	*Bosea*
	Species	**Bosea lupini**
	Full Scientific Name (LPSN)	Bosea lupini De Meyer and Willems 2012

Information on morphological and physiological properties Morphology

[Ref.: #23325]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.984

[Ref.: #23325]	Cell length	1.2-1.5 µm

[Ref.: #23325]	Cell width	0.8 µm

[Ref.: #23325]	Cell shape	rod-shaped

[Ref.: #23325]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %97.197

Information on culture and growth conditions Culture and growth conditions

[Ref.: #23325]

Culture medium

YMA

[Ref.: #23325]

Culture medium growth

[Ref.: #18270]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #18270]

Culture medium growth

[Ref.: #18270]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18270]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #18270]		growth	28 °C
[Ref.: #23325]		growth	25 °C
[Ref.: #23325]		growth	28 °C
[Ref.: #23325]		growth	33 °C
[Ref.: #23325]	no	growth	42 °C
[Ref.: #62589]		growth	30 °C

[Ref.: #18270]	Temperature range	mesophilic
[Ref.: #23325]	Temperature range	mesophilic
[Ref.: #23325]	Temperature range	thermophilic
[Ref.: #62589]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #62589]

Oxygen tolerance

aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.979

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23325]	adipate ChEBI	+/-	assimilation
[Ref.: #23325]	citrate ChEBI	+/-	assimilation
[Ref.: #23325]	D-glucose ChEBI	+/-	assimilation
[Ref.: #23325]	D-mannitol ChEBI	-	assimilation
[Ref.: #23325]	D-mannose ChEBI	+/-	assimilation
[Ref.: #23325]	L-arabinose ChEBI	+/-	assimilation
[Ref.: #23325]	malate ChEBI	+	assimilation
[Ref.: #23325]	maltose ChEBI	-	assimilation
[Ref.: #23325]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #23325]	nitrate ChEBI	-	reduction
[Ref.: #23325]	phenylacetate ChEBI	-	assimilation
[Ref.: #23325]	potassium gluconate ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #23325]	amoxicillin ChEBI		yes
[Ref.: #23325]	doxycycline ChEBI	yes
[Ref.: #23325]	penicillin ChEBI		yes

	Enzyme	Enzyme activity	EC number
[Ref.: #23325]	beta-glucosidase	+	3.2.1.21
[Ref.: #23325]	catalase	+	1.11.1.6
[Ref.: #23325]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23325]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23325]	Sample type/isolated from	root nodules of Lupinus polyphyllus
[Ref.: #23325]	Geographic location (country and/or sea, region)	Flanders
[Ref.: #23325]	Country	Belgium
[Ref.: #23325]	Country ISO 3 Code	BEL
[Ref.: #23325]	Continent	Europe

[Ref.: #62589]	Sample type/isolated from	Root nodule,Lupinus polyphyllus
[Ref.: #62589]	Sampling date	2008-09-10
[Ref.: #62589]	Geographic location (country and/or sea, region)	Flanders
[Ref.: #62589]	Country	Belgium
[Ref.: #62589]	Country ISO 3 Code	BEL
[Ref.: #62589]	Continent	Europe

[Ref.: #18270]	Sample type/isolated from	root nodules of Robinia pseudoacacia
[Ref.: #18270]	Geographic location (country and/or sea, region)	garden of the Ghent University
[Ref.: #18270]	Country	Belgium
[Ref.: #18270]	Country ISO 3 Code	BEL
[Ref.: #18270]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18270]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #18270]	Bosea sp. R-45681 partial 16S rRNA gene, strain R-45681	FR774992	1444	ENA	946785 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bosea lupini LMG 26383,CCUG 61248,R- 45681	GCA_900109525	contig	GenBank	1036779 tax ID	*
[Ref.: #66792]	Bosea lupini strain LMG 26383,CCUG 61248,R- 45681	1036779.5	wgs	PATRIC	1036779 tax ID	*
[Ref.: #66792]	Bosea lupini DSM 26673	2634166302	draft	JGI IMG/M	1036779 tax ID	*

[Ref.: #23325]

GC-content

66.9 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bosea lupini strain LMG 26383,CCUG 61248,R- 45681 PATRIC: 1036779.5
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bosea lupini strain LMG 26383,CCUG 61248,R- 45681 PATRIC: 1036779.5
[Ref.: #69480]	motile	yes	89.568	yes
[Ref.: #69480]	flagellated	no	59.436	no
[Ref.: #69480]	gram-positive	no	98.365	yes
[Ref.: #69480]	anaerobic	no	98.553	yes
[Ref.: #69480]	aerobic	yes	94.815	yes
[Ref.: #69480]	halophile	no	94.622	no
[Ref.: #69480]	spore-forming	no	94.321	no
[Ref.: #69480]	thermophile	no	97.504	no
[Ref.: #69480]	glucose-util	yes	92.534	no
[Ref.: #69480]	glucose-ferment	no	92.275	no

Availability in culture collections External links

[Ref.: #18270]

Culture collection no.

DSM 26673, CCUG 61248, LMG 26383

[Ref.: #71439]

SI-ID 371081

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bosea caraganae sp. nov. a new species of slow-growing bacteria isolated from root nodules of the relict species Caragana jubata (Pall.) Poir. originating from Mongolia.	Sazanova AL, Safronova VI, Kuznetsova IG, Karlov DS, Belimov AA, Andronov EE, Chirak ER, Popova JP, Verkhozina AV, Willems A, Tikhonovich IA	Int J Syst Evol Microbiol	10.1099/ijsem.0.003509	2019	*
Phylogeny	Bosea psychrotolerans sp. nov., a psychrotrophic alphaproteobacterium isolated from Lake Michigan water.	Albert RA, McGuine M, Pavlons SC, Roecker J, Bruess J, Mossman S, Sun S, King M, Hong S, Farrance CE, Danner J, Joung Y, Shapiro N, Whitman WB, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.003319	2019	*
Phylogeny	Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumes.	De Meyer SE, Willems A	Int J Syst Evol Microbiol	10.1099/ijs.0.035477-0	2011	*

References

#18270

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26673

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23325

Sofie E. De Meyer, Anne Willems: Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumes. IJSEM 62: 2505 - 2510 2012 ( DOI 10.1099/ijs.0.035477-0 , PubMed 22155761 )

#62589 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 61248

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71439

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID371081.1 )

* These data were automatically processed and therefore are not curated

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Bosea lupini strain LMG 26383,CCUG 61248,R- 45681

Bosea lupini strain LMG 26383,CCUG 61248,R- 45681

Change proposal

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