Name: Bosea vestrisii 34635
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1790

Type strain:

Species: Bosea vestrisii

Strain Designation: 34635

Culture col. no.: DSM 21229, CCUG 43114, CIP 106340, CCUG 43114

Strain history: 1999, B. La Scola, NRC, Fac. Medicine, Marseille, France: strain 34635

NCBI tax ID(s): 151416 (species)

Other strains from Bosea vestrisii

B. vestrisii CIP 106339, CCUG 43115
B. vestrisii CIP 106341, CCUG 43113

Section

Bosea vestrisii 34635 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from water supply of the La Timone Hospital Centre.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Boseaceae*
	Genus	*Bosea*
	Species	**Bosea vestrisii**
	Full Scientific Name (PNU)	Bosea vestrisii La Scola et al. 2003

Information on morphological and physiological properties Morphology

[Ref.: #23324]	Gram stain	negative
[Ref.: #23324]	Cell shape	rod-shaped
[Ref.: #23324]	Motility	yes
[Ref.: #23324]	Flagellum arrangement	monotrichous, polar

[Ref.: #55671]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15584]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #15584]

Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

[Ref.: #15584]

Culture medium composition

expand / minimize

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Culture medium

Columbia agar

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Culture medium growth

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Culture medium composition

expand / minimize

[Ref.: #23324]

Culture medium

MacConkey's agar

[Ref.: #23324]

Culture medium growth

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Culture medium composition

expand / minimize

[Ref.: #39500]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #39500]

Culture medium growth

[Ref.: #39500]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #15584]		growth	28 °C
[Ref.: #23324]	no	growth	42 °C
[Ref.: #23324]		growth	25 °C
[Ref.: #23324]		growth	37 °C
[Ref.: #39500]		growth	30 °C
[Ref.: #55671]		growth	30 °C

[Ref.: #15584]	Temperature range	mesophilic
[Ref.: #23324]	Temperature range	thermophilic
[Ref.: #23324]	Temperature range	mesophilic
[Ref.: #39500]	Temperature range	mesophilic
[Ref.: #55671]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #55671]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23324]		NaCl	no	growth	6	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23324]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23324]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23324]	adipate ChEBI	+	assimilation
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #23324]	amygdalin ChEBI	-	builds acid from
[Ref.: #23324]	arabinose ChEBI	+	assimilation
[Ref.: #23324]	arbutin ChEBI	-	builds acid from
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23324]	cellobiose ChEBI	-	builds acid from
[Ref.: #23324]	citrate ChEBI	+	assimilation
[Ref.: #23324]	D-arabinose ChEBI	-	builds acid from
[Ref.: #23324]	D-arabitol ChEBI	-	builds acid from
[Ref.: #23324]	D-fructose ChEBI	-	builds acid from
[Ref.: #23324]	D-fucose ChEBI	-	builds acid from
[Ref.: #23324]	D-galactose ChEBI	-	builds acid from
[Ref.: #23324]	D-glucose ChEBI	-	builds acid from
[Ref.: #23324]	D-glucose ChEBI	+	assimilation
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #23324]	D-lyxose ChEBI	-	builds acid from
[Ref.: #23324]	D-mannitol ChEBI	-	builds acid from
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #23324]	D-mannose ChEBI	-	builds acid from
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #23324]	D-ribose ChEBI	-	builds acid from
[Ref.: #23324]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #23324]	D-tagatose ChEBI	-	builds acid from
[Ref.: #23324]	D-trehalose ChEBI	-	builds acid from
[Ref.: #23324]	D-xylose ChEBI	-	builds acid from
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #23324]	erythritol ChEBI	-	builds acid from
[Ref.: #23324]	esculin ChEBI	-	builds acid from
[Ref.: #23324]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23324]	galactitol ChEBI	-	builds acid from
[Ref.: #23324]	gelatin ChEBI	-	hydrolysis
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23324]	gentiobiose ChEBI	-	builds acid from
[Ref.: #23324]	gluconate ChEBI	-	builds acid from
[Ref.: #23324]	gluconate ChEBI	+	assimilation
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23324]	glycerol ChEBI	-	builds acid from
[Ref.: #23324]	glycogen ChEBI	-	builds acid from
[Ref.: #23324]	inulin ChEBI	-	builds acid from
[Ref.: #23324]	L-arabinose ChEBI	-	builds acid from
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23324]	L-arabitol ChEBI	-	builds acid from
[Ref.: #23324]	L-fucose ChEBI	-	builds acid from
[Ref.: #23324]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #23324]	L-sorbose ChEBI	-	builds acid from
[Ref.: #23324]	L-xylose ChEBI	-	builds acid from
[Ref.: #23324]	lactose ChEBI	-	builds acid from
[Ref.: #23324]	malate ChEBI	+	assimilation
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23324]	maltose ChEBI	-	assimilation
[Ref.: #23324]	maltose ChEBI	-	builds acid from
[Ref.: #23324]	maltose ChEBI	+	assimilation
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #23324]	mannitol ChEBI	+	assimilation
[Ref.: #23324]	mannose ChEBI	+	assimilation
[Ref.: #23324]	melezitose ChEBI	-	builds acid from
[Ref.: #23324]	melibiose ChEBI	-	builds acid from
[Ref.: #23324]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #23324]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #23324]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #23324]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23324]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #23324]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #23324]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #23324]	phenylacetate ChEBI	-	assimilation
[Ref.: #23324]	raffinose ChEBI	-	builds acid from
[Ref.: #23324]	ribitol ChEBI	-	builds acid from
[Ref.: #23324]	salicin ChEBI	-	builds acid from
[Ref.: #23324]	starch ChEBI	-	builds acid from
[Ref.: #23324]	sucrose ChEBI	-	builds acid from
[Ref.: #23324]	thiosulfate ChEBI	-	oxidation
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #23324]	turanose ChEBI	-	builds acid from
[Ref.: #68369]	urea ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #23324]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #23324]	amikacin ChEBI	yes	4	µg/mL (MIC)
[Ref.: #23324]	amoxicillin ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	cefepime ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	cefoxitin ChEBI	yes	2	µg/mL (MIC)
[Ref.: #23324]	ceftazidime ChEBI	yes	128	µg/mL (MIC)
[Ref.: #23324]	ceftriaxone ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	cephalothin ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	ciprofloxacin ChEBI	yes	64	µg/mL (MIC)
[Ref.: #23324]	co-trimoxazole ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	colistin ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	doxycycline ChEBI	yes	0.25	µg/mL (MIC)
[Ref.: #23324]	erythromycin ChEBI	yes	8	µg/mL (MIC)
[Ref.: #23324]	gentamicin ChEBI	yes	32	µg/mL (MIC)
[Ref.: #23324]	imipenem ChEBI	yes	32	µg/mL (MIC)
[Ref.: #23324]	netilmycin ChEBI	yes	0.5	µg/mL (MIC)
[Ref.: #23324]	penicillin ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	piperacillin ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	rifampicin ChEBI	yes	16	µg/mL (MIC)
[Ref.: #23324]	ticarcillin ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	tobramycin ChEBI	yes	64	µg/mL (MIC)
[Ref.: #23324]	vancomycin ChEBI	yes	256	µg/mL (MIC)
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Group ID of combined antibiotics	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #23324]		12	amoxicillin ChEBI	yes	32	µg/mL (MIC)
[Ref.: #23324]		12	clavulanic acid ChEBI	yes	32	µg/mL (MIC)

	Metabolite production	Metabolite
[Ref.: #68369]		indole ChEBI	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #23324]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #23324]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #23324]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #23324]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #23324]	urease	+	3.5.1.5
[Ref.: #68369]	urease	+	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #55671]

Fatty acid	Percentage	ECL
C18:1 ω7c /12t/9t	56.7	17.824
C16:0	14	16
C19:0 CYCLO ω8c	9.8	18.9
C17:0 CYCLO	7.2	16.888
C16:1 ω5c	4.6	15.908
C16:1 ω7c	3.9	15.819
C16:0 3OH	3.3	17.52
C18:0	0.5	18

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #55671]	8003	+	-	-	-	+	-	-	-	-	+	-	-	-	-	+	-	-	+	+	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #55671]	4091	-	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23324]	Sample type/isolated from	water supply of the La Timone Hospital Centre
[Ref.: #23324]	Geographic location (country and/or sea, region)	Marseille
[Ref.: #23324]	Country	France
[Ref.: #23324]	Country ISO 3 Code	FRA
[Ref.: #23324]	Continent	Europe

[Ref.: #55671]	Sample type/isolated from	Water,hospital water supply

[Ref.: #15584]	Sample type/isolated from	Water, hospital water supply
[Ref.: #15584]	Geographic location (country and/or sea, region)	Marseille
[Ref.: #15584]	Country	France
[Ref.: #15584]	Country ISO 3 Code	FRA
[Ref.: #15584]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	-
#Infection	#Medical environment	#Clinic

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15584]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15584]	Alpha proteobacterium 34635 16S ribosomal RNA gene, partial sequence	AF288306	1451	ENA	1212487 tax ID

Availability in culture collections External links

[Ref.: #15584]

Culture collection no.

DSM 21229, CCUG 43114, CIP 106340, CCUG 43114

[Ref.: #20218]

Associated Passport(s) in StrainInfo

302114, 302115

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996).	La Scola B, Mallet MN, Grimont PAD, Raoult D	Int J Syst Evol Microbiol	10.1099/ijs.0.02127-0	2003	*
Phylogeny	Bosea psychrotolerans sp. nov., a psychrotrophic alphaproteobacterium isolated from Lake Michigan water.	Albert RA, McGuine M, Pavlons SC, Roecker J, Bruess J, Mossman S, Sun S, King M, Hong S, Farrance CE, Danner J, Joung Y, Shapiro N, Whitman WB, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.003319	2019	*

References

#15584

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21229

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23324

Bernard La Scola, Marie-Noelle Mallet, Patrick A. D. Grimont, Didier Raoult: Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996). IJSEM 53: 15 - 20 2003 ( DOI 10.1099/ijs.0.02127-0 , PubMed 12656146 )

#39500 ; Curators of the CIP;
#55671 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 43114 T

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

Change proposal

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