Name: Defluviitalea saccharophila 6LT2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17884

Type strain:

Species: Defluviitalea saccharophila

Strain Designation: 6LT2, LIND6LT2

Culture col. no.: DSM 22681, JCM 16312

Strain history: M.-L. Fardeau LIND6LT2.

NCBI tax ID(s): 879970 (species)

SI-ID 403556

DSM 22681

Section

Defluviitalea saccharophila 6LT2 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from upflow anaerobic filter treating abattoir wastewaters.

Gram-positive
spore-forming
anaerobe
thermophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Defluviitaleaceae*
	Genus	*Defluviitalea*
	Species	**Defluviitalea saccharophila**
	Full Scientific Name (LPSN)	Defluviitalea saccharophila Jabari et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #30206]	Gram stain	positive

[Ref.: #30206]	Cell length	05-10 µm

[Ref.: #30206]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16460]

Culture medium

DEFLUVIITALEA MEDIUM (DSMZ Medium 483)

[Ref.: #16460]

Culture medium growth

[Ref.: #16460]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16460]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16460]	growth	50 °C
[Ref.: #30206]	growth	45-60 °C
[Ref.: #30206]	optimum	50-55 °C
[Ref.: #67770]	growth	50 °C

[Ref.: #16460]	Temperature range	thermophilic
[Ref.: #30206]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #30206]			optimum	7-7.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16460]	Oxygen tolerance	anaerobe
[Ref.: #30206]	Oxygen tolerance	anaerobe

[Ref.: #30206]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30206]		NaCl		growth	0-2	%
[Ref.: #30206]		NaCl		optimum	0.50	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30206]	cellobiose ChEBI	+	carbon source
[Ref.: #30206]	glucose ChEBI	+	carbon source
[Ref.: #30206]	maltose ChEBI	+	carbon source
[Ref.: #30206]	mannitol ChEBI	+	carbon source
[Ref.: #30206]	mannose ChEBI	+	carbon source
[Ref.: #30206]	sucrose ChEBI	+	carbon source
[Ref.: #30206]	xylose ChEBI	+	carbon source

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16460]	Sample type/isolated from	upflow anaerobic filter treating abattoir wastewaters
[Ref.: #16460]	Country	Tunisia
[Ref.: #16460]	Country ISO 3 Code	TUN
[Ref.: #16460]	Continent	Africa

[Ref.: #67770]	Sample type/isolated from	Upflow anaerobic filter treating abattoir wastewaters in Tunisia
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Engineered product
#Engineered	#Waste	#Wastewater
#Condition	#Anoxic (anaerobic)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HQ020487 (>99% sequence identity) for Defluviitalea saccharophila subclade from Microbeatlas

Aquatic Samples	17
Soil Samples	39
Animal Samples	177
Plant Samples	39
Total Samples	272

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16460]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16460]	Defluviitalea saccharophila strain LIND6LT2 16S ribosomal RNA gene, partial sequence	HQ020487	1495	ENA	879970 tax ID

[Ref.: #16460]	GC-content	35.2 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30206]	GC-content	35.2 mol%

Availability in culture collections External links

[Ref.: #16460]

Culture collection no.

DSM 22681, JCM 16312

[Ref.: #86873]

SI-ID 403556

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Defluviitalea raffinosedens sp. nov., a thermophilic, anaerobic, saccharolytic bacterium isolated from an anaerobic batch digester treating animal manure and rice straw.	Ma S, Huang Y, Wang C, Fan H, Dai L, Zhou Z, Liu X, Deng Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.001664	2017	*
Metabolism	Defluviitalea phaphyphila sp. nov., a Novel Thermophilic Bacterium That Degrades Brown Algae.	Ji SQ, Wang B, Lu M, Li FL	Appl Environ Microbiol	10.1128/AEM.03297-15	2015	*
Phylogeny	Characterization of Defluviitalea saccharophila gen. nov., sp. nov., a thermophilic bacterium isolated from an upflow anaerobic filter treating abattoir wastewaters, and proposal of Defluviitaleaceae fam. nov.	Jabari L, Gannoun H, Cayol JL, Hamdi M, Fauque G, Ollivier B, Fardeau ML	Int J Syst Evol Microbiol	10.1099/ijs.0.030700-0	2011	*

References

#16460

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22681

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30206

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26551 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#86873

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403556.1 )

#26551

IJSEM 550 2012 ( DOI 10.1099/ijs.0.030700-0 , PubMed 21515711 )

* These data were automatically processed and therefore are not curated

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