Bosea eneae (DSM 21596, CCUG 43111, CIP 106338)

Name: Bosea eneae (DSM 21596, CCUG 43111, CIP 106338)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1788

Type strain

Strain Designation 34614

Culture col. no. DSM 21596 CCUG 43111 CIP 106338 34614

NCBI tax ID(s) 151454

Special Collections DSMZ CCUG CIP Literature strains

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Bosea eneae 34614 is an aerobe, mesophilic, Gram-negative prokaryote that forms circular colonies and was isolated from hospital water supplies of La Timone Hospital Centre.

Gram-negative motile rod-shaped colony-forming aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 100565

CCUG 43111,CIP 106338,LMG 26220,DSM 21596

@ref 20215

Last LPSN update 2025-12-16 09:47:11

Domain Pseudomonadati

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Boseaceae

Genus Bosea

Species Bosea eneae

Full scientific name Bosea eneae La Scola et al. 2003

BacDive ID	Other strains from **Bosea eneae** (2)	Type strain
23804	B. eneae CRIB-11, DSM 18165
138342	B. eneae 34617, CIP 106342, CCUG 43112

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Flagellum arrangement
23324	negative	rod-shaped		monotrichous, polar
119032	negative	rod-shaped

Colony morphology

@ref	Type of hemolysis	Hemolysis ability	Colony color	Colony shape	Incubation period	Medium used
23324	alpha	1	cream	circular	2-3 days	BCYE agar
55668					2-3 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
15778	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
23324	BCYE agar
23324	Columbia agar		5% sheep blood, 0.2% yeast extract
23324	MacConkey's agar
23324	NB
39499	MEDIUM 23 - for Afipia and Legionella		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
119032	CIP Medium 23	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
15778	positive	growth	28	mesophilic
23324	positive	growth	25	mesophilic
23324	positive	growth	37	mesophilic
23324	negative	growth	42
39499	positive	growth	30	mesophilic
55668	positive	growth	30	mesophilic
119032	positive	growth	22-30
119032	negative	growth	15
119032	negative	growth	37
119032	negative	growth	41

Physiology and metabolism

Oxygen tolerance

55668

Oxygen toleranceaerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23324	NaCl		growth	6 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23324	16808 ChEBI	2-dehydro-D-gluconate	-	builds acid from
23324	17426 ChEBI	5-dehydro-D-gluconate	-	builds acid from
23324	17128 ChEBI	adipate	+	assimilation
23324	27613 ChEBI	amygdalin	-	builds acid from
23324	22599 ChEBI	arabinose	-	assimilation
23324	18305 ChEBI	arbutin	-	builds acid from
23324	17057 ChEBI	cellobiose	-	builds acid from
23324	16947 ChEBI	citrate	-	assimilation
23324	17108 ChEBI	D-arabinose	-	builds acid from
23324	18333 ChEBI	D-arabitol	-	builds acid from
23324	15824 ChEBI	D-fructose	-	builds acid from
23324	28847 ChEBI	D-fucose	-	builds acid from
23324	12936 ChEBI	D-galactose	-	builds acid from
23324	17634 ChEBI	D-glucose	-	assimilation
23324	17634 ChEBI	D-glucose	-	builds acid from
23324	62318 ChEBI	D-lyxose	-	builds acid from
23324	16899 ChEBI	D-mannitol	-	builds acid from
23324	16024 ChEBI	D-mannose	-	builds acid from
23324	16988 ChEBI	D-ribose	-	builds acid from
23324	17924 ChEBI	D-sorbitol	-	builds acid from
23324	16443 ChEBI	D-tagatose	-	builds acid from
23324	16551 ChEBI	D-trehalose	-	builds acid from
23324	65327 ChEBI	D-xylose	-	builds acid from
23324	17113 ChEBI	erythritol	-	builds acid from
23324	4853 ChEBI	esculin	-	builds acid from
23324	4853 ChEBI	esculin	-	hydrolysis
23324	16813 ChEBI	galactitol	-	builds acid from
23324	5291 ChEBI	gelatin	-	hydrolysis
23324	28066 ChEBI	gentiobiose	-	builds acid from
23324	24265 ChEBI	gluconate	-	builds acid from
23324	24265 ChEBI	gluconate	+	assimilation
23324	17754 ChEBI	glycerol	-	builds acid from
23324	28087 ChEBI	glycogen	-	builds acid from
119032	606565 ChEBI	hippurate	+	hydrolysis
23324	15443 ChEBI	inulin	-	builds acid from
23324	30849 ChEBI	L-arabinose	-	builds acid from
23324	18403 ChEBI	L-arabitol	-	builds acid from
23324	18287 ChEBI	L-fucose	-	builds acid from
23324	62345 ChEBI	L-rhamnose	-	builds acid from
23324	17266 ChEBI	L-sorbose	-	builds acid from
23324	65328 ChEBI	L-xylose	-	builds acid from
23324	17716 ChEBI	lactose	-	builds acid from
23324	25115 ChEBI	malate	-	assimilation
23324	17306 ChEBI	maltose	-	assimilation
23324	17306 ChEBI	maltose	-	builds acid from
23324	29864 ChEBI	mannitol	-	assimilation
23324	37684 ChEBI	mannose	-	assimilation
23324	6731 ChEBI	melezitose	-	builds acid from
23324	28053 ChEBI	melibiose	-	builds acid from
23324	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from
23324	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from
23324	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from
23324	17268 ChEBI	myo-inositol	-	builds acid from
23324	506227 ChEBI	N-acetylglucosamine	-	assimilation
23324	506227 ChEBI	N-acetylglucosamine	-	builds acid from
23324	17632 ChEBI	nitrate	-	reduction
119032	17632 ChEBI	nitrate	-	reduction
119032	16301 ChEBI	nitrite	-	reduction
23324	18401 ChEBI	phenylacetate	-	assimilation
23324	16634 ChEBI	raffinose	-	builds acid from
23324	15963 ChEBI	ribitol	-	builds acid from
23324	17814 ChEBI	salicin	-	builds acid from
23324	28017 ChEBI	starch	-	builds acid from
23324	17992 ChEBI	sucrose	-	builds acid from
23324	16094 ChEBI	thiosulfate	-	oxidation
23324	32528 ChEBI	turanose	-	builds acid from
23324	17151 ChEBI	xylitol	-	builds acid from

Antibiotic resistance

@ref	Metabolite	Is antibiotic	Is sensitive	Is resistant
119032	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
23324	amikacin
23324	amoxicillin
23324	amoxicillin
23324	cefepime
23324	cefoxitin
23324	ceftazidime
23324	ceftriaxone
23324	cephalothin
23324	ciprofloxacin
23324	clavulanic acid
23324	co-trimoxazole
23324	colistin
23324	doxycycline
23324	erythromycin
23324	gentamicin
23324	imipenem
23324	netilmycin
23324	penicillin
23324	piperacillin
23324	rifampicin
23324	ticarcillin
23324	tobramycin
23324	vancomycin

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119032	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
119032	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
23324	arginine dihydrolase	-	3.5.3.6
23324	beta-galactosidase	-	3.2.1.23
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
23324	catalase	+	1.11.1.6
119032	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
23324	cytochrome oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
119032	gamma-glutamyltransferase	-	2.3.2.2
119032	gelatinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
119032	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119032	ornithine decarboxylase	-	4.1.1.17
119032	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
23324	urease	+	3.5.1.5
119032	urease	+	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119032	-	+	+	+	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater
#Infection	#Medical environment	#Clinic

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
15778	hospital water supplies of La Timone Hospital Centre	Marseilles	France	FRA	Europe
55668	Water,hospital water supply
119032	Environment, Hospital water supplies

Taxonmaps

69479

Global distribution of 16S sequence AF288300 (>99% sequence identity) for Bosea from Microbeatlas

Aquatic Samples	435
Soil Samples	165
Animal Samples	235
Plant Samples	413
Total Samples	1248

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15778	1	Risk group (German classification) According to TRBA 466
119032	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM4265726v1 assembly for Bosea eneae CCUG 43111	scaffold	GCA_042657265	151454	48.25

Genome browser: GCA_042657265

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15778	Alpha proteobacterium 34614 16S ribosomal RNA gene, partial sequence	AF288300	1446		1211774

GC content

@ref	GC-content (mol%)	Method
23324	69.0	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Usefulness of rpoB gene sequencing for identification of Afipia and Bosea species, including a strategy for choosing discriminative partial sequences.	Khamis A, Colson P, Raoult D, Scola BL.	Appl Environ Microbiol	10.1128/aem.69.11.6740-6749.2003	2003
Phylogeny	Bosea psychrotolerans sp. nov., a psychrotrophic alphaproteobacterium isolated from Lake Michigan water.	Albert RA, McGuine M, Pavlons SC, Roecker J, Bruess J, Mossman S, Sun S, King M, Hong S, Farrance CE, Danner J, Joung Y, Shapiro N, Whitman WB, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.003319	2019
Phylogeny	Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996).	La Scola B, Mallet MN, Grimont PAD, Raoult D	Int J Syst Evol Microbiol	10.1099/ijs.0.02127-0	2003

References

#15778	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21596
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23324	Bernard La Scola, Marie-Noelle Mallet, Patrick A. D. Grimont, Didier Raoult: Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996). IJSEM 53: 15 - 20 2003 ( DOI 10.1099/ijs.0.02127-0 , PubMed 12656146 )
#39499	; Curators of the CIP;
#55668	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 43111
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119032	Collection of Institut Pasteur ; Curators of the CIP; CIP 106338
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Bosea eneae (DSM 21596, CCUG 43111, CIP 106338)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_042657265

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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