Name: Bosea eneae 34614
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1788

Type strain:

Species: Bosea eneae

Strain Designation: 34614

Culture col. no.: DSM 21596, CCUG 43111, CIP 106338

Strain history: CIP <- 1999, B. La Scola, NRC, Fac. Medicine, Marseille, France: strain 34614

NCBI tax ID(s): 151454 (species)

SI-ID 100565

CCUG 43111, CIP 106338, LMG 26220, DSM 21596

Other strains from Bosea eneae

B. eneae CRIB-11, DSM 18165
B. eneae 34617, CIP 106342, CCUG 43112

Section

Bosea eneae 34614 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from hospital water supplies of La Timone Hospital Centre.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Boseaceae*
	Genus	*Bosea*
	Species	**Bosea eneae**
	Full Scientific Name (LPSN)	Bosea eneae La Scola et al. 2003

Information on morphological and physiological properties Morphology

[Ref.: #23324]	Gram stain	negative
[Ref.: #119032]	Gram stain	negative

[Ref.: #23324]	Cell shape	rod-shaped
[Ref.: #119032]	Cell shape	rod-shaped

[Ref.: #23324]	Motility	yes
[Ref.: #119032]	Motility	yes

[Ref.: #23324]	Flagellum arrangement	monotrichous, polar

[Ref.: #23324]	Type of hemolysis	alpha
[Ref.: #23324]	Hemolysis ability	1
[Ref.: #23324]	Colony color	cream
[Ref.: #23324]	Colony shape	circular
[Ref.: #23324]	Incubation period	2-3 days
[Ref.: #23324]	Cultivation medium used	BCYE agar

[Ref.: #55668]	Incubation period	2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15778]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #15778]

Culture medium growth

[Ref.: #15778]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15778]

Culture medium composition

expand / minimize

[Ref.: #23324]

Culture medium

BCYE agar

[Ref.: #23324]

Culture medium growth

[Ref.: #23324]

Culture medium

Columbia agar

[Ref.: #23324]

Culture medium growth

[Ref.: #23324]

Culture medium composition

expand / minimize

[Ref.: #23324]

Culture medium

MacConkey's agar

[Ref.: #23324]

Culture medium growth

[Ref.: #23324]

Culture medium

[Ref.: #23324]

Culture medium growth

[Ref.: #39499]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #39499]

Culture medium growth

[Ref.: #39499]

Culture medium composition

expand / minimize

[Ref.: #119032]

Culture medium

CIP Medium 23

[Ref.: #119032]

Culture medium growth

[Ref.: #119032]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #15778]		growth	28 °C
[Ref.: #23324]		growth	25 °C
[Ref.: #23324]		growth	37 °C
[Ref.: #23324]	no	growth	42 °C
[Ref.: #39499]		growth	30 °C
[Ref.: #55668]		growth	30 °C
[Ref.: #119032]		growth	22-30 °C
[Ref.: #119032]	no	growth	15 °C
[Ref.: #119032]	no	growth	37 °C
[Ref.: #119032]	no	growth	41 °C

[Ref.: #15778]	Temperature range	mesophilic
[Ref.: #23324]	Temperature range	mesophilic
[Ref.: #23324]	Temperature range	thermophilic
[Ref.: #39499]	Temperature range	mesophilic
[Ref.: #55668]	Temperature range	mesophilic
[Ref.: #119032]	Temperature range	psychrophilic
[Ref.: #119032]	Temperature range	mesophilic
[Ref.: #119032]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #55668]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23324]		NaCl	no	growth	6	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23324]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23324]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23324]	adipate ChEBI	+	assimilation
[Ref.: #23324]	amygdalin ChEBI	-	builds acid from
[Ref.: #23324]	arabinose ChEBI	-	assimilation
[Ref.: #23324]	arbutin ChEBI	-	builds acid from
[Ref.: #23324]	cellobiose ChEBI	-	builds acid from
[Ref.: #23324]	citrate ChEBI	-	assimilation
[Ref.: #23324]	D-arabinose ChEBI	-	builds acid from
[Ref.: #23324]	D-arabitol ChEBI	-	builds acid from
[Ref.: #23324]	D-fructose ChEBI	-	builds acid from
[Ref.: #23324]	D-fucose ChEBI	-	builds acid from
[Ref.: #23324]	D-galactose ChEBI	-	builds acid from
[Ref.: #23324]	D-glucose ChEBI	-	assimilation
[Ref.: #23324]	D-glucose ChEBI	-	builds acid from
[Ref.: #23324]	D-lyxose ChEBI	-	builds acid from
[Ref.: #23324]	D-mannitol ChEBI	-	builds acid from
[Ref.: #23324]	D-mannose ChEBI	-	builds acid from
[Ref.: #23324]	D-ribose ChEBI	-	builds acid from
[Ref.: #23324]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #23324]	D-tagatose ChEBI	-	builds acid from
[Ref.: #23324]	D-trehalose ChEBI	-	builds acid from
[Ref.: #23324]	D-xylose ChEBI	-	builds acid from
[Ref.: #23324]	erythritol ChEBI	-	builds acid from
[Ref.: #23324]	esculin ChEBI	-	builds acid from
[Ref.: #23324]	esculin ChEBI	-	hydrolysis
[Ref.: #23324]	galactitol ChEBI	-	builds acid from
[Ref.: #23324]	gelatin ChEBI	-	hydrolysis
[Ref.: #23324]	gentiobiose ChEBI	-	builds acid from
[Ref.: #23324]	gluconate ChEBI	-	builds acid from
[Ref.: #23324]	gluconate ChEBI	+	assimilation
[Ref.: #23324]	glycerol ChEBI	-	builds acid from
[Ref.: #23324]	glycogen ChEBI	-	builds acid from
[Ref.: #119032]	hippurate ChEBI	+	hydrolysis
[Ref.: #23324]	inulin ChEBI	-	builds acid from
[Ref.: #23324]	L-arabinose ChEBI	-	builds acid from
[Ref.: #23324]	L-arabitol ChEBI	-	builds acid from
[Ref.: #23324]	L-fucose ChEBI	-	builds acid from
[Ref.: #23324]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #23324]	L-sorbose ChEBI	-	builds acid from
[Ref.: #23324]	L-xylose ChEBI	-	builds acid from
[Ref.: #23324]	lactose ChEBI	-	builds acid from
[Ref.: #23324]	malate ChEBI	-	assimilation
[Ref.: #23324]	maltose ChEBI	-	assimilation
[Ref.: #23324]	maltose ChEBI	-	builds acid from
[Ref.: #23324]	mannitol ChEBI	-	assimilation
[Ref.: #23324]	mannose ChEBI	-	assimilation
[Ref.: #23324]	melezitose ChEBI	-	builds acid from
[Ref.: #23324]	melibiose ChEBI	-	builds acid from
[Ref.: #23324]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #23324]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #23324]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #23324]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23324]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #23324]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #23324]	nitrate ChEBI	-	reduction
[Ref.: #119032]	nitrate ChEBI	-	reduction
[Ref.: #119032]	nitrite ChEBI	-	reduction
[Ref.: #23324]	phenylacetate ChEBI	-	assimilation
[Ref.: #23324]	raffinose ChEBI	-	builds acid from
[Ref.: #23324]	ribitol ChEBI	-	builds acid from
[Ref.: #23324]	salicin ChEBI	-	builds acid from
[Ref.: #23324]	starch ChEBI	-	builds acid from
[Ref.: #23324]	sucrose ChEBI	-	builds acid from
[Ref.: #23324]	thiosulfate ChEBI	-	oxidation
[Ref.: #23324]	turanose ChEBI	-	builds acid from
[Ref.: #23324]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance
[Ref.: #119032]	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no			yes
[Ref.: #23324]	amikacin ChEBI	yes	8	µg/mL (MIC)
[Ref.: #23324]	amoxicillin ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	cefepime ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	cefoxitin ChEBI	yes	2	µg/mL (MIC)
[Ref.: #23324]	ceftazidime ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	ceftriaxone ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	cephalothin ChEBI	yes	128	µg/mL (MIC)
[Ref.: #23324]	ciprofloxacin ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	co-trimoxazole ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	colistin ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	doxycycline ChEBI	yes	1	µg/mL (MIC)
[Ref.: #23324]	erythromycin ChEBI	yes	64	µg/mL (MIC)
[Ref.: #23324]	gentamicin ChEBI	yes	128	µg/mL (MIC)
[Ref.: #23324]	imipenem ChEBI	yes	64	µg/mL (MIC)
[Ref.: #23324]	netilmycin ChEBI	yes	2	µg/mL (MIC)
[Ref.: #23324]	penicillin ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	piperacillin ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	rifampicin ChEBI	yes	4	µg/mL (MIC)
[Ref.: #23324]	ticarcillin ChEBI	yes	256	µg/mL (MIC)
[Ref.: #23324]	tobramycin ChEBI	yes	64	µg/mL (MIC)
[Ref.: #23324]	vancomycin ChEBI	yes	256	µg/mL (MIC)
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Group ID of combined antibiotics	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #23324]		12	amoxicillin ChEBI	yes	32	µg/mL (MIC)
[Ref.: #23324]		12	clavulanic acid ChEBI	yes	32	µg/mL (MIC)

	Metabolite production	Metabolite	Production
[Ref.: #119032]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #119032]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #23324]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23324]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #23324]	catalase	+	1.11.1.6
[Ref.: #119032]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #23324]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #119032]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #119032]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119032]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119032]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119032]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #23324]	urease	+	3.5.1.5
[Ref.: #119032]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119032]	9414	-	+	+	+	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15778]	Sample type/isolated from	hospital water supplies of La Timone Hospital Centre
[Ref.: #15778]	Geographic location (country and/or sea, region)	Marseilles
[Ref.: #15778]	Country	France
[Ref.: #15778]	Country ISO 3 Code	FRA
[Ref.: #15778]	Continent	Europe

[Ref.: #55668]	Sample type/isolated from	Water,hospital water supply

[Ref.: #119032]	Sample type/isolated from	Environment, Hospital water supplies
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Freshwater
#Infection	#Medical environment	#Clinic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF288300 (>99% sequence identity) for Bosea from Microbeatlas

Aquatic Samples	435
Soil Samples	165
Animal Samples	235
Plant Samples	413
Total Samples	1248

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15778]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119032]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15778]	Alpha proteobacterium 34614 16S ribosomal RNA gene, partial sequence	AF288300	1446	ENA	1211774 tax ID

[Ref.: #23324]

GC-content

69.0 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #15778]

Culture collection no.

DSM 21596, CCUG 43111, CIP 106338

[Ref.: #71435]

SI-ID 100565

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bosea psychrotolerans sp. nov., a psychrotrophic alphaproteobacterium isolated from Lake Michigan water.	Albert RA, McGuine M, Pavlons SC, Roecker J, Bruess J, Mossman S, Sun S, King M, Hong S, Farrance CE, Danner J, Joung Y, Shapiro N, Whitman WB, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.003319	2019	*
Phylogeny	Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996).	La Scola B, Mallet MN, Grimont PAD, Raoult D	Int J Syst Evol Microbiol	10.1099/ijs.0.02127-0	2003	*

References

#15778

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21596

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23324

Bernard La Scola, Marie-Noelle Mallet, Patrick A. D. Grimont, Didier Raoult: Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996). IJSEM 53: 15 - 20 2003 ( DOI 10.1099/ijs.0.02127-0 , PubMed 12656146 )

#39499 ; Curators of the CIP;
#55668 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 43111

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71435

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID100565.1 )

#119032 Collection of Institut Pasteur ; Curators of the CIP; CIP 106338
* These data were automatically processed and therefore are not curated

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