Name: Amorphus orientalis DSM 21202
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17874

Type strain:

Species: Amorphus orientalis

Culture col. no.: DSM 21202, CCTCC AA 208035, YIM D10

Strain history: <- X.-L. Cui, YIM; YIM D10 <- J.-H. Liu

NCBI tax ID(s): 649198 (species)

SI-ID 407935

DSM 21202

Section

Amorphus orientalis DSM 21202 is an aerobe, mesophilic, Gram-negative bacterium that produces polysaccharides and was isolated from salt mine sediment.

polysaccharide production
Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Amorphaceae*
	Genus	*Amorphus*
	Species	**Amorphus orientalis**
	Full Scientific Name (LPSN)	Amorphus orientalis Wang et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #29550]	Gram stain	negative

[Ref.: #29550]	Cell length	1-2.5 µm

[Ref.: #29550]	Cell width	1-2.5 µm

[Ref.: #29550]	Cell shape	rod-shaped

[Ref.: #29550]	Motility	no

[Ref.: #29550]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15576]

Culture medium

ISP MEDIUM 2 WITH 5% NaCl (DSMZ Medium 636)

[Ref.: #15576]

Culture medium growth

[Ref.: #15576]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15576]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #15576]	growth	28 °C
[Ref.: #29550]	growth	15-37 °C
[Ref.: #29550]	optimum	28 °C

[Ref.: #15576]	Temperature range	mesophilic
[Ref.: #29550]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29550]			optimum	7.5
[Ref.: #29550]			growth	6.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29550]

Oxygen tolerance

aerobe

[Ref.: #15576]

Name of produced compound

exopolysaccharide

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29550]		NaCl		growth	0.5-12	%
[Ref.: #29550]		NaCl		optimum	03-08	%

[Ref.: #29550]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29550]	acetate ChEBI	+	carbon source
[Ref.: #29550]	arabinose ChEBI	+	carbon source
[Ref.: #29550]	citrate ChEBI	+	carbon source
[Ref.: #29550]	dextrin ChEBI	+	carbon source
[Ref.: #29550]	glucose ChEBI	+	carbon source
[Ref.: #29550]	glycerol ChEBI	+	carbon source
[Ref.: #29550]	maltose ChEBI	+	carbon source
[Ref.: #29550]	mannitol ChEBI	+	carbon source
[Ref.: #29550]	mannose ChEBI	+	carbon source
[Ref.: #29550]	succinate ChEBI	+	carbon source
[Ref.: #29550]	sucrose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #29550]		catalase	+	1.11.1.6
[Ref.: #29550]		urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15576]	Sample type/isolated from	salt mine sediment
[Ref.: #15576]	Geographic location (country and/or sea, region)	Yunnan, Fenggang salt mine
[Ref.: #15576]	Country	China
[Ref.: #15576]	Country ISO 3 Code	CHN
[Ref.: #15576]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Other	#Mine
#Environmental	#Terrestrial	#Sediment
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FJ998414 (>99% sequence identity) for Amorphus orientalis subclade from Microbeatlas

Aquatic Samples	45
Soil Samples	38
Animal Samples	6
Plant Samples	3
Total Samples	92

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15576]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15576]	Amorphus orientalis strain YIM D10 16S ribosomal RNA gene, partial sequence	FJ998414	1385	ENA	649198 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Amorphus orientalis DSM 21202	2926584956	draft	JGI IMG/M	649198 tax ID	*

[Ref.: #15576]	GC-content	58.6 mol%	fluorimetric
[Ref.: #15576]	GC-content	67.1 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29550]	GC-content	65.4 mol%

Availability in culture collections External links

[Ref.: #15576]

Culture collection no.

DSM 21202, CCTCC AA 208035, YIM D10

[Ref.: #86863]

SI-ID 407935

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudoxanthobacter liyangensis sp. nov., isolated from dichlorodiphenyltrichloroethane-contaminated soil.	Liu XM, Chen K, Meng C, Zhang L, Zhu JC, Huang X, Li SP, Jiang JD	Int J Syst Evol Microbiol	10.1099/ijs.0.056507-0	2014	*
Phylogeny	Amorphus suaedae sp. nov., isolated from the root of a tidal flat plant, Suaeda maritima.	Hwang JM, Chung EJ, Park JA, Jeong JH, Jeon CO, Chung YR	Int J Syst Evol Microbiol	10.1099/ijs.0.048959-0	2013	*
Phylogeny	Amorphus orientalis sp. nov., an exopolysaccharide-producing bacterium isolated from salt mine sediment.	Wang YX, Liu JH, Chen YG, Zhang XX, Wang ZG, Chen Y, Tian SP, Hu B, Cui XL	Int J Syst Evol Microbiol	10.1099/ijs.0.015735-0	2009	*

References

#15576

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21202

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29550

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25945 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#86863

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407935.1 )

#25945

IJSEM 1750 2010 ( DOI 10.1099/ijs.0.015735-0 , PubMed 19749037 )

* These data were automatically processed and therefore are not curated

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