Leptotrichia wadei (DSM 19758, CCUG 47505, CIP 107918)

Name: Leptotrichia wadei (DSM 19758, CCUG 47505, CIP 107918)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17867

Type strain

Strain Designation LB 16

Culture col. no. DSM 19758 CCUG 47505 CIP 107918 LB 16 JCM 16777

NCBI tax ID(s) 1122174 157687

Special Collections DSMZ CCUG JCM CIP Literature strains

Links

Leptotrichia wadei LB 16 is an anaerobe, mesophilic, Gram-negative prokaryote that was isolated from human saliva.

Gram-negative rod-shaped anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 131424

CCUG 47505,CIP 107918,DSM 19758,JCM 16777

@ref 20215

Last LPSN update 2025-12-16 09:51:12

Domain Fusobacteriati

Phylum Fusobacteriota

Class Fusobacteriia

Order Fusobacteriales

Family Leptotrichiaceae

Genus Leptotrichia

Species Leptotrichia wadei

Full scientific name Leptotrichia wadei Eribe et al. 2004

BacDive ID	Other strains from **Leptotrichia wadei** (2)	Type strain
154308	L. wadei CCUG 53009
156621	L. wadei CCUG 63608

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
30006	negative	6.25 µm	0.45 µm	rod-shaped
123897	negative			rod-shaped

Colony morphology

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8298	PYG MEDIUM (MODIFIED) (DSMZ Medium 104)	Medium recipe at MediaDive	Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
8298	COLUMBIA BLOOD AGAR (DSMZ Medium 429)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429) Composition: Horse blood 40.0 g/l Columbia agar base
34293	MEDIUM 6 - Columbia agar with 10 % horse blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
123897	CIP Medium 6	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8298	positive	growth	37	mesophilic
30006	positive	optimum	37	mesophilic
34293	positive	growth	37	mesophilic
57725	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
8298	anaerobe
30006	anaerobe
57725	anaerobe
123897	facultative anaerobe
125439	anaerobe	98

Spore formation

30006

Spore formationno

Observation

30006

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
123897	17057 ChEBI	cellobiose	+	degradation
123897	17108 ChEBI	D-arabinose	-	degradation
123897	15824 ChEBI	D-fructose	+	degradation
123897	17634 ChEBI	D-glucose	+	degradation
123897	65327 ChEBI	D-xylose	-	degradation
30006	4853 ChEBI	esculin	+	hydrolysis
123897	4853 ChEBI	esculin	+	hydrolysis
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
123897	17716 ChEBI	lactose	-	degradation
123897	17306 ChEBI	maltose	+	degradation
123897	17632 ChEBI	nitrate	-	reduction
123897	17632 ChEBI	nitrate	+	respiration
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
123897	16301 ChEBI	nitrite	-	reduction
123897	17814 ChEBI	salicin	-	degradation
123897	17992 ChEBI	sucrose	+	degradation
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
123897	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68380	35581 ChEBI	indole		from API rID32A
123897	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	-	from API rID32A

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68380	alanine arylamidase	-	3.4.11.2	from API rID32A
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68380	alkaline phosphatase	+	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68380	alpha-galactosidase	-	3.2.1.22	from API rID32A
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68380	alpha-glucosidase	+	3.2.1.20	from API rID32A
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123897	amylase	-
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
68382	beta-galactosidase	-	3.2.1.23	from API zym
123897	beta-galactosidase	-	3.2.1.23
68380	beta-galactosidase	-	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68382	beta-glucosidase	-	3.2.1.21	from API zym
68380	beta-glucosidase	+	3.2.1.21	from API rID32A
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
123897	caseinase	-	3.4.21.50
30006	catalase	+	1.11.1.6
123897	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
123897	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
123897	gelatinase	-
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	-		from API rID32A
68380	histidine arylamidase	-		from API rID32A
68380	L-arginine arylamidase	-		from API rID32A
123897	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68380	leucine arylamidase	-	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
123897	lipase	-
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
123897	oxidase	-
68380	phenylalanine arylamidase	-		from API rID32A
68380	proline-arylamidase	-	3.4.11.5	from API rID32A
123897	protease	-
68380	pyrrolidonyl arylamidase	+	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
68382	trypsin	-	3.4.21.4	from API zym
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
123897	tween esterase	-
68380	tyrosine arylamidase	-		from API rID32A
123897	urease	-	3.5.1.5
68380	urease	-	3.5.1.5	from API rID32A
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123897	-	+	+	+	-	+	-	-	-	-	+	-	-	-	-	+	-	-	-	-

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
8298	-	-	-	-	-	+	+	-	-	-	+/-	+/-	-	-	-	-	+	-	-	-	-	-	+	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Human	-
#Host Body Product	#Oral cavity and Airways	#Saliva

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
8298	human saliva		Norway	NOR	Europe
57725	Human saliva,healthy person	Bergen	Norway	NOR	Europe
67770	Saliva of a healthy person
123897	Human, Saliva

Taxonmaps

69479

Global distribution of 16S sequence AB588021 (>99% sequence identity) for Leptotrichia wadei subclade from Microbeatlas

Aquatic Samples	971
Soil Samples	667
Animal Samples	34375
Plant Samples	239
Total Samples	36252

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8298	1	Risk group (German classification) According to TRBA 466
123897	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM799054v2 assembly for Leptotrichia wadei JCM16777	complete	GCA_007990545	157687.272	8075178977	157687	99.26
66792	Leptotrichia wadei strain JCM16777	contig		157687.179		157687	79.04
67770	ASM37334v1 assembly for Leptotrichia wadei DSM 19758	scaffold	GCA_000373345	1122174.3	2515154120	1122174	66.75

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
8298	Leptotrichia wadeii strain LB16 16S ribosomal RNA gene, partial sequence	AY029802	1486		157687
67770	Leptotrichia wadei gene for 16S ribosomal RNA, partial sequence, strain: JCM 16777	AB588021	1473		157687

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Leptotrichia wadei DSM 19758
NCBI: GCA_000373345

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	98.00	no
125439	gram_stain	BacteriaNetⓘ	variable	78.70	no
125439	motility	BacteriaNetⓘ	yes	72.80	no
125439	spore_formation	BacteriaNetⓘ	yes	79.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Leptotrichia wadei JCM16777
NCBI: GCA_007990545.2

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	88.20	yes
125438	anaerobic	anaerobicⓘ	yes	68.45	no
125438	aerobic	aerobicⓘ	no	93.50	yes
125438	spore-forming	spore-formingⓘ	no	89.52	yes
125438	thermophilic	thermophileⓘ	no	92.97	yes
125438	flagellated	motile2+ⓘ	no	86.17	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Phylogenetic and comparative genomics of the family Leptotrichiaceae and introduction of a novel fingerprinting MLVA for Streptobacillus moniliformis.	Eisenberg T, Fawzy A, Nicklas W, Semmler T, Ewers C.	BMC Genomics	10.1186/s12864-016-3206-0	2016
	Selenomonas sputigena acts as a pathobiont mediating spatial structure and biofilm virulence in early childhood caries.	Cho H, Ren Z, Divaris K, Roach J, Lin BM, Liu C, Azcarate-Peril MA, Simancas-Pallares MA, Shrestha P, Orlenko A, Ginnis J, North KE, Zandona AGF, Ribeiro AA, Wu D, Koo H.	Nat Commun	10.1038/s41467-023-38346-3	2023
Phylogeny	Genetic diversity of Leptotrichia and description of Leptotrichia goodfellowii sp. nov., Leptotrichia hofstadii sp. nov., Leptotrichia shahii sp. nov. and Leptotrichia wadei sp. nov.	Eribe ERK, Paster BJ, Caugant DA, Dewhirst FE, Stromberg VK, Lacy GH, Olsen I	Int J Syst Evol Microbiol	10.1099/ijs.0.02819-0	2004

References

#8298	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19758
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26370	IJSEM 583 2004 ( DOI 10.1099/ijs.0.02819-0 , PubMed 15023979 )
#30006	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26370
#34293	; Curators of the CIP;
#57725	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47505
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68380	Automatically annotated from API rID32A .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123897	Collection of Institut Pasteur ; Curators of the CIP; CIP 107918
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Leptotrichia wadei (DSM 19758, CCUG 47505, CIP 107918)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API rID32A

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_007990545

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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