Name: Leptotrichia hofstadii L48
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17865

Type strain:

Species: Leptotrichia hofstadii

Strain Designation: L48, LB 23

Culture col. no.: DSM 21651, CCUG 47504, CIP 107917, JCM 16775

Strain history: CCUG 47504 <-- I. Olsen LB 23 <-- T. Hofstad.

NCBI tax ID(s): 1122171 (strain), 157688 (species)

SI-ID 131422

CCUG 47504, CIP 107917, JCM 16775

Section

Leptotrichia hofstadii L48 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from human saliva, healthy person.

Gram-negative
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Fusobacteriota*
	Class	*Fusobacteriia*
	Order	*Fusobacteriales*
	Family	*Leptotrichiaceae*
	Genus	*Leptotrichia*
	Species	**Leptotrichia hofstadii**
	Full Scientific Name (LPSN)	Leptotrichia hofstadii Eribe et al. 2004

Information on morphological and physiological properties Morphology

[Ref.: #30006]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.7

[Ref.: #30006]	Cell length	11.25 µm

[Ref.: #30006]	Cell width	0.7 µm

[Ref.: #30006]	Cell shape	rod-shaped

[Ref.: #30006]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15867]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #15867]

Culture medium growth

[Ref.: #15867]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15867]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #15867]	growth	37 °C
[Ref.: #30006]	optimum	37 °C
[Ref.: #57724]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #15867]	Temperature range	mesophilic
[Ref.: #30006]	Temperature range	mesophilic
[Ref.: #57724]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #15867]	Oxygen tolerance	anaerobe
[Ref.: #30006]	Oxygen tolerance	anaerobe
[Ref.: #57724]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %98.648

[Ref.: #30006]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.956

[Ref.: #30006]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #30006]	esculin ChEBI	+	hydrolysis
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #30006]	mannose ChEBI	+	carbon source
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #30006]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	-	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #30006]	catalase	+	1.11.1.6
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #15867]	12391	-	-	-	-	-	+	+	-	-	-	+/-	+/-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15867]	Sample type/isolated from	human saliva, healthy person
[Ref.: #15867]	Geographic location (country and/or sea, region)	Bergen
[Ref.: #15867]	Country	Norway
[Ref.: #15867]	Country ISO 3 Code	NOR
[Ref.: #15867]	Continent	Europe

[Ref.: #57724]	Sample type/isolated from	Human saliva,healthy person
[Ref.: #57724]	Geographic location (country and/or sea, region)	Bergen
[Ref.: #57724]	Country	Norway
[Ref.: #57724]	Country ISO 3 Code	NOR
[Ref.: #57724]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Saliva of a healthy person
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Oral cavity and Airways	#Saliva

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB558170 (>99% sequence identity) for Leptotrichia hofstadii subclade from Microbeatlas

Aquatic Samples	384
Soil Samples	145
Animal Samples	9416
Plant Samples	93
Total Samples	10038

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15867]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Leptotrichia hofstadii gene for 16S ribosomal RNA, partial sequence, strain: JCM 16775	AB558170	1473	ENA	157688 tax ID
[Ref.: #15867]	Leptotrichia hofstadii strain LB23 16S ribosomal RNA gene, partial sequence	AY029803	1486	ENA	157688 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Leptotrichia hofstadii DSM 21651	GCA_000428965	scaffold	GenBank	1122171 tax ID
[Ref.: #66792]	Leptotrichia hofstadii JCM16775	GCA_007990525	complete	GenBank	157688 tax ID	*
[Ref.: #66792]	Leptotrichia hofstadii DSM 21651	1122171.3	wgs	PATRIC	1122171 tax ID	*
[Ref.: #66792]	Leptotrichia hofstadii strain JCM16775	157688.7	wgs	PATRIC	157688 tax ID	*
[Ref.: #66792]	Leptotrichia hofstadii JCM16775	2914873612	complete	JGI IMG/M	157688 tax ID	*
[Ref.: #66792]	Leptotrichia hofstadii DSM 21651	2524614766	draft	JGI IMG/M	1122171 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Leptotrichia hofstadii JCM16775 NCBI: GCA_007990525.1
[Ref.: #69481]	spore-forming	no	83	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Leptotrichia hofstadii JCM16775 NCBI: GCA_007990525.1
[Ref.: #69480]	motile	no	95.282	yes
[Ref.: #69480]	flagellated	no	97.111	yes
[Ref.: #69480]	gram-positive	no	94.022	yes
[Ref.: #69480]	anaerobic	yes	96.939	yes
[Ref.: #69480]	aerobic	no	98.845	yes
[Ref.: #69480]	halophile	no	80.514	no
[Ref.: #69480]	spore-forming	no	95.629	yes
[Ref.: #69480]	glucose-util	yes	74.483	no
[Ref.: #69480]	thermophile	no	95.588	no
[Ref.: #69480]	glucose-ferment	yes	67.01	no

Availability in culture collections External links

[Ref.: #15867]

Culture collection no.

DSM 21651, CCUG 47504, CIP 107917, JCM 16775

[Ref.: #86855]

SI-ID 131422

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Genetic diversity of Leptotrichia and description of Leptotrichia goodfellowii sp. nov., Leptotrichia hofstadii sp. nov., Leptotrichia shahii sp. nov. and Leptotrichia wadei sp. nov.	Eribe ERK, Paster BJ, Caugant DA, Dewhirst FE, Stromberg VK, Lacy GH, Olsen I	Int J Syst Evol Microbiol	10.1099/ijs.0.02819-0	2004	*

References

#15867

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21651

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30006

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26370 (see below)

#57724 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47504

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86855

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID131422.1 )

#26370

IJSEM 583 2004 ( DOI 10.1099/ijs.0.02819-0 , PubMed 15023979 )

* These data were automatically processed and therefore are not curated

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