Leptotrichia buccalis (DSM 1135, ATCC 14201, JCM 12969)

Name: Leptotrichia buccalis (DSM 1135, ATCC 14201, JCM 12969)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17860

Type strain

Strain Designation C-1013-b

Culture col. no. DSM 1135 ATCC 14201 JCM 12969 NCTC 10249 CCUG 34316 2 more

NCBI tax ID(s) 523794 40542

Special Collections DSMZ CCUG JCM CIP

History DSMZ <- NCTC <- A. Howell, Jr., C-1013-b JCM <-- DSM 1135 <-- NCTC 10249 <-- A. Howell, Jr. C-1013-b. CIP <- 1998, CCUG <- R.J. Owen, NCTC, London, UK <- A. Howell, NIH, Maryland, USA: strain C-1013-b

Links

Leptotrichia buccalis C-1013-b is an anaerobe, Gram-positive, rod-shaped human pathogen that was isolated from supragingival calculus.

Gram-positive rod-shaped anaerobe human pathogen genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 35996

NCTC 14386,ATCC 14201,NCTC 10249,CCUG 34316,CIP 105792,DSM 1135,JCM 12969

@ref 20215

Last LPSN update 2026-05-29 08:29:06

Domain Bacteria

Phylum Fusobacteriota

Class Fusobacteriia

Order Fusobacteriales

Family Leptotrichiaceae

Genus Leptotrichia

Species Leptotrichia buccalis

Full scientific name Leptotrichia buccalis (Robin 1853) Trevisan 1879 (Approved Lists 1980)

Synonyms (1)

"Leptothrix buccalis"

BacDive ID	Other strains from **Leptotrichia buccalis** (30)	Type strain
143422	L. buccalis CCUG 19067 B
143448	L. buccalis CCUG 19169
143468	L. buccalis CCUG 19427
143703	L. buccalis CCUG 21070
144226	L. buccalis CCUG 23775
145825	L. buccalis CCUG 29716
146221	L. buccalis CCUG 30637
146785	L. buccalis CCUG 32116
146816	L. buccalis CCUG 32173
147370	L. buccalis CCUG 33471
147815	L. buccalis CCUG 34278
147990	L. buccalis CCUG 34631
148812	L. buccalis CCUG 35982
149041	L. buccalis CCUG 36771
149112	L. buccalis CCUG 36868
149165	L. buccalis CCUG 36980
149672	L. buccalis CCUG 38157
150594	L. buccalis CCUG 41699
150900	L. buccalis CCUG 42664
151367	L. buccalis CCUG 43884
151902	L. buccalis CCUG 45152 A
151903	L. buccalis CCUG 45152 B
152024	L. buccalis CCUG 45578
152227	L. buccalis CCUG 46170
152427	L. buccalis CCUG 46767
152533	L. buccalis CCUG 47013
152936	L. buccalis CCUG 48068
153772	L. buccalis CCUG 50932
156218	L. buccalis CCUG 60755
156363	L. buccalis CCUG 61290

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
117066	positive	rod-shaped

Colony morphology

@ref	Incubation period	Hemolysis ability
575	1-2 days
52043	2 days
117066		0

Multimedia

@ref:	66793
multimedia content:	EM_DSM_1135_1.jpg
multimedia.multimedia content:	EM_DSM_1135_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
575	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
575	CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)	Medium recipe at MediaDive	Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water
575	PYG MEDIUM (MODIFIED) (DSMZ Medium 104)	Medium recipe at MediaDive	Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
39366	MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
117066	CIP Medium 10	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
575	positive	growth	37
39366	positive	growth	37
52043	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
575	anaerobe
52043	anaerobe
52043	microaerophile
117066	anaerobe
125439	anaerobe	98.439

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68377	15824 ChEBI	D-fructose	+	builds acid from	from API NH
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68377	17634 ChEBI	D-glucose	+	builds acid from	from API NH
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	+	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
117066	4853 ChEBI	esculin	+	hydrolysis
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	+	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	+	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	+	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68377	17306 ChEBI	maltose	+	builds acid from	from API NH
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
117066	17632 ChEBI	nitrate	-	reduction
117066	17632 ChEBI	nitrate	+	respiration
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
117066	16301 ChEBI	nitrite	-	reduction
68377	18257 ChEBI	ornithine	-	degradation	from API NH
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	+	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
68377	27897 ChEBI	tryptophan	-	energy source	from API NH
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A
68377	16199 ChEBI	urea	-	hydrolysis	from API NH
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite
68380	35581 ChEBI	indole	from API rID32A
68377	35581 ChEBI	indole	from API NH
117066	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	-	from API rID32A
68377	35581 ChEBI	indole	-	from API NH

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68380	alanine arylamidase	-	3.4.11.2	from API rID32A
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68380	alkaline phosphatase	+	3.1.3.1	from API rID32A
68377	alkaline phosphatase	-	3.1.3.1	from API NH
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
117066	amylase	-
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
68382	beta-galactosidase	-	3.2.1.23	from API zym
117066	beta-galactosidase	-	3.2.1.23
68380	beta-galactosidase	-	3.2.1.23	from API rID32A
68377	beta-galactosidase	-	3.2.1.23	from API NH
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
117066	caseinase	+	3.4.21.50
117066	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
117066	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
117066	gelatinase	-
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	-		from API rID32A
68380	histidine arylamidase	-		from API rID32A
68380	L-arginine arylamidase	-		from API rID32A
117066	lecithinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
68380	leucine arylamidase	-	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
117066	lipase	-
68377	lipase	-		from API NH
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68377	ornithine decarboxylase	-	4.1.1.17	from API NH
117066	oxidase	-
68380	phenylalanine arylamidase	-		from API rID32A
68380	proline-arylamidase	-	3.4.11.5	from API rID32A
117066	protease	-
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
68382	trypsin	-	3.4.21.4	from API zym
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
68377	tryptophan deaminase	-	4.1.99.1	from API NH
117066	tween esterase	-
68380	tyrosine arylamidase	-		from API rID32A
117066	urease	-	3.5.1.5
68380	urease	-	3.5.1.5	from API rID32A
68377	urease	-	3.5.1.5	from API NH
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	formaldehyde oxidation	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	L-lactaldehyde degradation	100	3 of 3
66794	palmitate biosynthesis	95.45	21 of 22
66794	threonine metabolism	90	9 of 10
66794	lipid A biosynthesis	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	isoleucine metabolism	87.5	7 of 8
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	gluconeogenesis	87.5	7 of 8
66794	vitamin B12 metabolism	85.29	29 of 34
66794	peptidoglycan biosynthesis	80	12 of 15
66794	starch degradation	80	8 of 10
66794	glycogen metabolism	80	4 of 5
66794	photosynthesis	78.57	11 of 14
66794	valine metabolism	77.78	7 of 9
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	CMP-KDO biosynthesis	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	acetate fermentation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	ppGpp biosynthesis	75	3 of 4
66794	flavin biosynthesis	73.33	11 of 15
66794	NAD metabolism	72.22	13 of 18
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	glycolysis	70.59	12 of 17
66794	purine metabolism	70.21	66 of 94
66794	phenylalanine metabolism	69.23	9 of 13
66794	pyrimidine metabolism	66.67	30 of 45
66794	d-mannose degradation	66.67	6 of 9
66794	acetoin degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	methionine metabolism	65.38	17 of 26
66794	heme metabolism	64.29	9 of 14
66794	oxidative phosphorylation	63.74	58 of 91
66794	methylglyoxal degradation	60	3 of 5
66794	lipoate biosynthesis	60	3 of 5
66794	tetrahydrofolate metabolism	57.14	8 of 14
66794	polyamine pathway	56.52	13 of 23
66794	degradation of sugar alcohols	56.25	9 of 16
66794	non-pathway related	55.26	21 of 38
66794	metabolism of disaccharids	54.55	6 of 11
66794	arginine metabolism	54.17	13 of 24
66794	leucine metabolism	53.85	7 of 13
66794	vitamin B1 metabolism	53.85	7 of 13
66794	sulfate reduction	53.85	7 of 13
66794	histidine metabolism	51.72	15 of 29
66794	adipate degradation	50	1 of 2
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	Entner Doudoroff pathway	50	5 of 10
66794	biotin biosynthesis	50	2 of 4
66794	cysteine metabolism	50	9 of 18
66794	glycine metabolism	50	5 of 10
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	phenylmercury acetate degradation	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	propionate fermentation	50	5 of 10
66794	butanoate fermentation	50	2 of 4
66794	ketogluconate metabolism	50	4 of 8
66794	tryptophan metabolism	47.37	18 of 38
66794	urea cycle	46.15	6 of 13
66794	proline metabolism	45.45	5 of 11
66794	lipid metabolism	45.16	14 of 31
66794	alanine metabolism	44.83	13 of 29
66794	CO2 fixation in Crenarchaeota	44.44	4 of 9
66794	molybdenum cofactor biosynthesis	44.44	4 of 9
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	mevalonate metabolism	42.86	3 of 7
66794	citric acid cycle	42.86	6 of 14
66794	lysine metabolism	42.86	18 of 42
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	propanol degradation	42.86	3 of 7
66794	bacilysin biosynthesis	40	2 of 5
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	glutamate and glutamine metabolism	39.29	11 of 28
66794	d-xylose degradation	36.36	4 of 11
66794	vitamin B6 metabolism	36.36	4 of 11
66794	tyrosine metabolism	35.71	5 of 14
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	glycolate and glyoxylate degradation	33.33	2 of 6
66794	degradation of hexoses	33.33	6 of 18
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	degradation of pentoses	32.14	9 of 28
66794	glutathione metabolism	28.57	4 of 14
66794	pentose phosphate pathway	27.27	3 of 11
66794	isoprenoid biosynthesis	26.92	7 of 26
66794	toluene degradation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	androgen and estrogen metabolism	25	4 of 16

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
117066	-	+	+	+	-	-	-	-	-	-	+	+	-	-	-	+	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
117066	not determinedn.d.	+	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	-	+	+	+	+	+	+	+	+	+	-	-	+	+	+	-	+	+	-	+	-	-	-	-	+	-	-

API NH

@ref	PEN	GLU	FRU	MAL	SAC	ODC	URE	LIP	PAL	beta GAL	ProA	GGT	IND
575	+	+	+	+	-	-	-	-	-	-	+/-	-	-
575	-	+	+	+	+	-	-	-	-	-	-	+/-	-

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
575	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-
575	-	-	+/-	-	+/-	+	+/-	-	-	-	+/-	+/-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Host Body-Site	#Oral cavity and airways

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent	Host species
575	supragingival calculus	USA	USA	North America
52043	Human supragingival calcus				Homo sapiens
67770	Supragingival calculus
117066	Human, Supragingival calculus				Homo sapiens

Taxonmaps

69479

Global distribution of 16S sequence AB818949 (>99% sequence identity) for Leptotrichia buccalis subclade from Microbeatlas

Aquatic Samples	674
Soil Samples	710
Animal Samples	27734
Plant Samples	186
Total Samples	29304

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Pathogenicity animal	Biosafety level	Biosafety level comment
575			2	Risk group (German classification) According to TRBA 466
117066			1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2390v1 assembly for Leptotrichia buccalis C-1013-b DSM 1135	complete	GCA_000023905	523794.5	644736384	523794	99.54
66792	Leptotrichia buccalis ATCC 14201	complete			2500645350	523794	0

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Leptotrichia buccalis gene for 16S ribosomal RNA, partial sequence, strain: JCM 12969	AB818949	1473	523794
20218	Leptotrichia buccalis strain ATCC 14201 16S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF342860	716	523794
67770	Leptotrichia buccalis 16S rRNA gene	X90831	1327	523794

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM2390v1 assembly for Leptotrichia buccalis C-1013-b DSM 1135
BV-BRC: 523794.5

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	98.44	no
125439	motility	BacteriaNetⓘ	yes	58.18	no
125439	gram_stain	BacteriaNetⓘ	positive	52.40	no
125439	spore_formation	BacteriaNetⓘ	yes	49.94	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Leptotrichia buccalis C-1013-b DSM 1135
NCBI: GCA_000023905.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	88.48	no
125438	anaerobic	anaerobicⓘ	yes	71.48	no
125438	aerobic	aerobicⓘ	no	95.32	yes
125438	spore-forming	spore-formingⓘ	no	82.83	no
125438	thermophilic	thermophileⓘ	no	93.34	yes
125438	flagellated	motile2+ⓘ	no	86.17	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Comparative Genomic Analysis of Fusobacterium necrophorum Provides Insights into Conserved Virulence Genes.	Bista PK, Pillai D, Roy C, Scaria J, Narayanan SK.	Microbiol Spectr	10.1128/spectrum.00297-22	2022
	CRISPR-Cas13 Inhibitors Block RNA Editing in Bacteria and Mammalian Cells.	Lin P, Qin S, Pu Q, Wang Z, Wu Q, Gao P, Schettler J, Guo K, Li R, Li G, Huang C, Wei Y, Gao GF, Jiang J, Wu M.	Mol Cell	10.1016/j.molcel.2020.03.033	2020
Genetics	A comprehensive appraisal of mechanism of anti-CRISPR proteins: an advanced genome editor to amend the CRISPR gene editing.	Choudhary N, Tandi D, Verma RK, Yadav VK, Dhingra N, Ghosh T, Choudhary M, Gaur RK, Abdellatif MH, Gacem A, Eltayeb LB, Alqahtani MS, Yadav KK, Jeon BH.	Front Plant Sci	10.3389/fpls.2023.1164461	2023
Enzymology	Fusobacterium nucleatum and Bacteroides fragilis detection in colorectal tumours: Optimal target site and correlation with total bacterial load.	Rye MS, Garrett KL, Holt RA, Platell CF, McCoy MJ.	PLoS One	10.1371/journal.pone.0262416	2022
Phylogeny	Illumina MiSeq 16S amplicon sequence analysis of bovine respiratory disease associated bacteria in lung and mediastinal lymph node tissue.	Johnston D, Earley B, Cormican P, Murray G, Kenny DA, Waters SM, McGee M, Kelly AK, McCabe MS.	BMC Vet Res	10.1186/s12917-017-1035-2	2017
	Diverse CRISPRs evolving in human microbiomes.	Rho M, Wu YW, Tang H, Doak TG, Ye Y.	PLoS Genet	10.1371/journal.pgen.1002441	2012
Phylogeny	Phylogenetic and comparative genomics of the family Leptotrichiaceae and introduction of a novel fingerprinting MLVA for Streptobacillus moniliformis.	Eisenberg T, Fawzy A, Nicklas W, Semmler T, Ewers C.	BMC Genomics	10.1186/s12864-016-3206-0	2016
	Veillonella, Firmicutes: Microbes disguised as Gram negatives.	Vesth T, Ozen A, Andersen SC, Kaas RS, Lukjancenko O, Bohlin J, Nookaew I, Wassenaar TM, Ussery DW.	Stand Genomic Sci	10.4056/sigs.2981345	2013
	Types I and V Anti-CRISPR Proteins: From Phage Defense to Eukaryotic Synthetic Gene Circuits.	Yu L, Marchisio MA.	Front Bioeng Biotechnol	10.3389/fbioe.2020.575393	2020
	Evaluation of microbiota associated with Herpesviruses in active sites of generalized aggressive periodontitis.	Passariello C, Gigola P, Testarelli L, Puttini M, Schippa S, Petti S.	Ann Stomatol (Roma)	10.11138/ads/2017.8.2.071	2017
Enzymology	Evolution of DNA ligases of nucleo-cytoplasmic large DNA viruses of eukaryotes: a case of hidden complexity.	Yutin N, Koonin EV.	Biol Direct	10.1186/1745-6150-4-51	2009
	Analytical Performance Characteristics of a New Transcription-Mediated Amplification Assay for Treponema pallidum.	Getman D, Lin M, Barakat N, Skvoretz R, Godornes C, Swenson P, Nenninger A, Golden MR, Lukehart SA.	J Clin Microbiol	10.1128/jcm.00511-21	2021
	A Novel Thermal-Activated beta-Galactosidase from Bacillus aryabhattai GEL-09 for Lactose Hydrolysis in Milk.	Luan S, Duan X.	Foods	10.3390/foods11030372	2022
Metabolism	An uncharacterized FMAG_01619 protein from Fusobacterium mortiferum ATCC 9817 demonstrates that some bacterial macrodomains can also act as poly-ADP-ribosylhydrolases.	Garcia-Saura AG, Zapata-Perez R, Hidalgo JF, Cabanes J, Gil-Ortiz F, Sanchez-Ferrer A.	Sci Rep	10.1038/s41598-019-39691-4	2019
Pathogenicity	Spatial Ecology of the Human Tongue Dorsum Microbiome.	Wilbert SA, Mark Welch JL, Borisy GG.	Cell Rep	10.1016/j.celrep.2020.02.097	2020
Genetics	Complete genome sequence of Bacillus velezensis ZY-1-1 reveals the genetic basis for its hemicellulosic/cellulosic substrate-inducible xylanase and cellulase activities.	Zhang ZY, Raza MF, Zheng Z, Zhang X, Dong X, Zhang H.	3 Biotech	10.1007/s13205-018-1490-x	2018
Pathogenicity	Effect of smokeless tobacco products on human oral bacteria growth and viability.	Liu M, Jin J, Pan H, Feng J, Cerniglia CE, Yang M, Chen H.	Anaerobe	10.1016/j.anaerobe.2016.10.006	2016
Pathogenicity	Molecular Assay for Detection of Genetic Markers Associated with Decreased Susceptibility to Cephalosporins in Neisseria gonorrhoeae.	Peterson SW, Martin I, Demczuk W, Bharat A, Hoang L, Wylie J, Allen V, Lefebvre B, Tyrrell G, Horsman G, Haldane D, Garceau R, Wong T, Mulvey MR.	J Clin Microbiol	10.1128/jcm.00493-15	2015
Phylogeny	Use of chemotaxonomy as an aid to differentiate among Capnocytophaga species, CDC group DF-3, and aerotolerant strains of Leptotrichia buccalis.	Bernard K, Cooper C, Tessier S, Ewan EP.	J Clin Microbiol	10.1128/jcm.29.10.2263-2265.1991	1991
	Effects of azithromycin, metronidazole, amoxicillin, and metronidazole plus amoxicillin on an in vitro polymicrobial subgingival biofilm model.	Soares GM, Teles F, Starr JR, Feres M, Patel M, Martin L, Teles R.	Antimicrob Agents Chemother	10.1128/aac.04974-14	2015
Enzymology	Abilities of the mCP Agar method and CRENAME alpha toxin-specific real-time PCR assay to detect Clostridium perfringens spores in drinking water.	Maheux AF, Berube E, Boudreau DK, Villeger R, Cantin P, Boissinot M, Bissonnette L, Bergeron MG.	Appl Environ Microbiol	10.1128/aem.02791-13	2013
	The vaginal microflora in relation to gingivitis.	Persson R, Hitti J, Verhelst R, Vaneechoutte M, Persson R, Hirschi R, Weibel M, Rothen M, Temmerman M, Paul K, Eschenbach D.	BMC Infect Dis	10.1186/1471-2334-9-6	2009
	Evaluation of the oxyrase OxyPlate anaerobe incubation system.	Wiggs LS, Cavallaro JJ, Miller JM.	J Clin Microbiol	10.1128/jcm.38.2.499-507.2000	2000
Metabolism	Ability of bacteria associated with chronic inflammatory disease to stimulate E-selectin expression and promote neutrophil adhesion.	Darveau RP, Cunningham MD, Bailey T, Seachord C, Ratcliffe K, Bainbridge B, Dietsch M, Page RC, Aruffo A.	Infect Immun	10.1128/iai.63.4.1311-1317.1995	1995
Enzymology	Evaluation of an immunofluorescent-antibody test for rapid identification of Pseudomonas aeruginosa in blood cultures.	Counts GW, Schwartz RW, Ulness BK, Hamilton DJ, Rosok MJ, Cunningham MD, Tam MR, Darveau RP.	J Clin Microbiol	10.1128/jcm.26.6.1161-1165.1988	1988
	Immunological cross-reactivity of oral non-streptococcal bacteria with mammalian tissue.	Stinson MW, Nisengard RJ, Pelonero L, Drinnan AJ.	Infect Immun	10.1128/iai.24.2.532-538.1979	1979
Enzymology	A comparative study of spirochaetes from the porcine alimentary tract.	Lemcke RM, Burrows MR.	J Hyg (Lond)	10.1017/s0022172400068881	1981
Metabolism	Metabolism of sugars by genetically diverse species of oral Leptotrichia.	Thompson J, Pikis A	Mol Oral Microbiol	10.1111/j.2041-1014.2011.00627.x	2011
Genetics	Complete genome sequence of Leptotrichia buccalis type strain (C-1013-b).	Ivanova N, Gronow S, Lapidus A, Copeland A, Glavina Del Rio T, Nolan M, Lucas S, Chen F, Tice H, Cheng JF, Saunders E, Bruce D, Goodwin L, Brettin T, Detter JC, Han C, Pitluck S, Mikhailova N, Pati A, Mavrommatis K, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Chain P, Rohde C, Goker M, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP	Stand Genomic Sci	10.4056/sigs.1854	2009
Enzymology	Purification and some properties of phospho-beta-galactosidase from the Gram-negative oral bacterium Leptotrichia buccalis ATCC 14201.	Thompson J	FEMS Microbiol Lett	10.1111/j.1574-6968.2002.tb11344.x	2002
Metabolism	Oligosaccharides obtained by partial hydrolysis of lipopolysaccharides from Leptotrichia buccalis.	Birkeland NK, Hofstad T	Scand J Dent Res	10.1111/j.1600-0722.1985.tb01335.x	1985
Enzymology	Purification and quantitative chemical analysis of cell wall peptidoglycans of Leptotrichia buccalis.	Fukuhara H, Umemoto T, Sagawa H, Kato K, Kotani S	Infect Immun	10.1128/iai.39.1.132-136.1983	1983
Phylogeny	Chemical composition, ultrastructure and some serological properties of lipopolysaccharides from Leptotrichia buccalis.	Birkeland NK, Hofstad T	Acta Pathol Microbiol Immunol Scand B	10.1111/j.1699-0463.1982.tb00127.x	1982
	Description of three new Leptotrichia species isolated from dental biofilm: Leptotrichia rugosa sp. nov., Leptotrichia mesophila sp. nov. and Leptotrichia alba sp. nov.	Wang X, Jiao SY, Wang J, Wu RR, Zhang TT, Wang CM, Li XJ.	Anaerobe	10.1016/j.anaerobe.2025.102948	2025

References

#575	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1135
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#39366	; Curators of the CIP;
#52043	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 34316
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68377	Automatically annotated from API NH .
#68380	Automatically annotated from API rID32A .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#117066	Collection of Institut Pasteur ; Curators of the CIP; CIP 105792
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
575	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-
575	-	-	+/-	-	+/-	+	+/-	-	-	-	+/-	+/-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
575	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-
575	-	-	+/-	-	+/-	+	+/-	-	-	-	+/-	+/-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-

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Leptotrichia buccalis (DSM 1135, ATCC 14201, JCM 12969)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

API zym

API 50CHac

API NH

API rID32A

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000023905

16S sequences

Genome-based predictions

Predictions

Literature

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References

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@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
575	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-
575	-	-	+/-	-	+/-	+	+/-	-	-	-	+/-	+/-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-