Methanolinea mesophila (DSM 23604, NBRC 105659, TNR)

Name: Methanolinea mesophila (DSM 23604, NBRC 105659, TNR)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17854

Type strain

Strain Designation TNR

Culture col. no. DSM 23604 NBRC 105659 TNR

NCBI tax ID(s) 547055

Special Collections DSMZ Literature strains

History <- I.-C. Tseng, National Cheng Kung University, Dept. of Life Sciences, Tainan, Taiwan; TNR <- S. Sakai and H. Imachi, Japan Agency for Marine-Earth Sci. and Technol. (JAMSTEC), Extremobiosphere Res. Center, Yokosuka, Japan; {2005}

Links

Methanolinea mesophila TNR is an anaerobe, rod-shaped archaeon that was isolated from rice field soil.

rod-shaped anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 397688

NBRC 105659,DSM 23604

@ref 20215

Last LPSN update 2026-02-09 11:22:19

Domain Archaea

Phylum Methanobacteriota

Class "Methanomicrobia"

Order Methanomicrobiales

Family Methanoregulaceae

Genus Methanolinea

Species Methanolinea mesophila

Full scientific name Methanolinea mesophila Sakai et al. 2012

Morphology

Cell morphology

@ref	Cell length	Cell width	Cell shape	Motility
30394	4.25 µm	0.3 µm	rod-shaped

Colony morphology

17235

Incubation period>14 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
17235	PELOTOMACULUM MEDIUM (DSMZ Medium 960)		Medium recipe at MediaDive	Name: PELOTOMACULUM MEDIUM (DSMZ Medium 960; with strain-specific modifications) Composition: Na2CO3 1.49701 g/l NH4Cl 0.538922 g/l L-Cysteine HCl x H2O 0.299401 g/l Na2S x 9 H2O 0.299401 g/l MgCl2 x 6 H2O 0.199601 g/l KH2PO4 0.139721 g/l Na acetate 0.0998004 g/l Yeast extract 0.0998004 g/l Nitrilotriacetic acid 0.0127745 g/l NaCl 0.000998004 g/l FeCl2 x 4 H2O 0.000998004 g/l Sodium resazurin 0.000499002 g/l Pyridoxine hydrochloride 9.98004e-05 g/l ZnCl2 9.98004e-05 g/l CaCl2 x 2 H2O 9.98004e-05 g/l NiCl2 x 6 H2O 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Na2WO4 x 2 H2O 3.99202e-05 g/l CoCl2 x 6 H2O 2.99401e-05 g/l Na2SeO3 x 5 H2O 2.99401e-05 g/l Na2MoO4 x 2 H2O 2.99401e-05 g/l CuCl2 1.99601e-05 g/l Biotin 1.99601e-05 g/l Folic acid 1.99601e-05 g/l H3BO3 9.98004e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
17235	positive	growth	37
30394	positive	growth	20-40
30394	positive	optimum	37

Culture pH

@ref	Ability	Type	PH
30394	positive	optimum	7
30394	positive	growth	6.5-7.4

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
17235	anaerobe
30394	anaerobe
125439	anaerobe	99.9

Metabolite production

@ref	Chebi-ID	Metabolite	Production
30394	16183 ChEBI	methane

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Agriculture	#Field
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
17235	rice field soil	Tainan	Taiwan, Province of China	TWN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AB447467 (>99% sequence identity) for Methanolinea mesophila subclade from Microbeatlas

Aquatic Samples	249
Soil Samples	221
Animal Samples	4
Plant Samples	16
Total Samples	490

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1787385v1 assembly for Methanolinea mesophila DSM 23604	contig	GCA_017873855	547055.3	2913416594	547055	76.42

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17235	Methanolinea mesophila gene for 16S ribosomal RNA, partial sequence	AB447467	1397		547055

GC content

@ref	GC-content (mol%)	Method
17235	56.4	high performance liquid chromatography (HPLC)
30394	56.4

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methanolinea mesophila DSM 23604
NCBI: GCA_017873855

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	64.20	no
125439	motility	BacteriaNetⓘ	no	54.80	no
125439	gram_stain	BacteriaNetⓘ	negative	79.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.90	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanolinea mesophila sp. nov., a hydrogenotrophic methanogen isolated from rice field soil, and proposal of the archaeal family Methanoregulaceae fam. nov. within the order Methanomicrobiales.	Sakai S, Ehara M, Tseng IC, Yamaguchi T, Brauer SL, Cadillo-Quiroz H, Zinder SH, Imachi H	Int J Syst Evol Microbiol	10.1099/ijs.0.035048-0	2011

References

#17235	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23604
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26731	IJSEM 1389 2012 ( DOI 10.1099/ijs.0.035048-0 , PubMed 21841010 )
#30394	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26731
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanolinea mesophila (DSM 23604, NBRC 105659, TNR)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Metabolite production

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_017873855

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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