Name: Demequina salsinemoris KV-810
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17839

Type strain:

Species: Demequina salsinemoris

Strain Designation: KV-810

Culture col. no.: DSM 22060, NBRC 105323

Strain history: <- Y. Takahashi, Kitasato Univ., Tokyo, Japan; KV-810

NCBI tax ID(s): 577470 (species)

SI-ID 406075

DSM 22060

Section

Demequina salsinemoris KV-810 is an aerobe, mesophilic, rod-shaped bacterium that was isolated from mangrove soil.

rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Demequinaceae*
	Genus	*Demequina*
	Species	**Demequina salsinemoris**
	Full Scientific Name (LPSN)	Demequina salsinemoris Matsumoto et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #29412]	Cell length	0.9 µm

[Ref.: #29412]	Cell width	0.35 µm

[Ref.: #29412]	Cell shape	rod-shaped

[Ref.: #21365]	Colony color	Sand yellow (1002)
[Ref.: #21365]	Incubation period	10-14 days
[Ref.: #21365]	Cultivation medium used	ISP 2

[Ref.: #21365]	Colony color	Signal yellow (1003)
[Ref.: #21365]	Incubation period	10-14 days
[Ref.: #21365]	Cultivation medium used	ISP 3

[Ref.: #29412]

Pigment production

yes

[Ref.: #21365]	Multicellular complex forming ability	no
[Ref.: #21365]	Medium Name (multicellularity)	ISP 2

[Ref.: #21365]	Multicellular complex forming ability	no
[Ref.: #21365]	Medium Name (multicellularity)	ISP 3

[Ref.: #21365]	Multicellular complex forming ability	no
[Ref.: #21365]	Medium Name (multicellularity)	ISP 4

[Ref.: #21365]	Multicellular complex forming ability	no
[Ref.: #21365]	Medium Name (multicellularity)	ISP 5

[Ref.: #21365]	Multicellular complex forming ability	no
[Ref.: #21365]	Medium Name (multicellularity)	ISP 7

[Ref.: #21365]	Multicellular complex forming ability	no
[Ref.: #21365]	Medium Name (multicellularity)	Suter with tyrosine

[Ref.: #21365]	Multicellular complex forming ability	no
[Ref.: #21365]	Medium Name (multicellularity)	Suter without tyrosine

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16047]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #16047]

Culture medium growth

[Ref.: #16047]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16047]

Culture medium composition

expand / minimize

[Ref.: #21365]

Culture medium

ISP 2

[Ref.: #21365]

Culture medium growth

[Ref.: #21365]

Culture medium composition

expand / minimize

[Ref.: #21365]

Culture medium

ISP 3

[Ref.: #21365]

Culture medium growth

[Ref.: #21365]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #16047]			growth	28 °C
[Ref.: #29412]			growth	12-34 °C

[Ref.: #16047]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #29412]			growth	05-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29412]

Oxygen tolerance

aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %91
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.996

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #21365]		NaCl		maximum	5	%
[Ref.: #29412]		NaCl		growth	<8	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #21365]	arabinose ChEBI	+
[Ref.: #29412]	arabinose ChEBI	+	carbon source
[Ref.: #29412]	cellobiose ChEBI	+	carbon source
[Ref.: #21365]	cellulose ChEBI	-
[Ref.: #68379]	D-glucose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #29412]	esculin ChEBI	+	hydrolysis
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #21365]	fructose ChEBI	+
[Ref.: #29412]	fructose ChEBI	+	carbon source
[Ref.: #29412]	galactose ChEBI	+	carbon source
[Ref.: #21365]	glucose ChEBI	+
[Ref.: #29412]	glucose ChEBI	+	carbon source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #29412]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #21365]	mannose ChEBI	+
[Ref.: #29412]	mannose ChEBI	+	carbon source
[Ref.: #21365]	myo-inositol ChEBI	-
[Ref.: #21365]	raffinose ChEBI	-
[Ref.: #21365]	rhamnose ChEBI	-
[Ref.: #29412]	rhamnose ChEBI	+	carbon source
[Ref.: #29412]	salicin ChEBI	+	carbon source
[Ref.: #21365]	sucrose ChEBI	-
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #21365]	xylose ChEBI	+
[Ref.: #29412]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #29412]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #29412]	alpha-galactosidase	+	3.2.1.22
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #29412]	catalase	+	1.11.1.6
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #21365]	494	+/-	-	-	-	-	+	+	-	+	+/-	+/-	not determinedn.d.	+	-	-	+/-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #21365]	1006	not determinedn.d.	-	+	+	-	+	+/-	+/-	-	-	-	+/-	+	+	-	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16047]	Sample type/isolated from	mangrove soil
[Ref.: #16047]	Geographic location (country and/or sea, region)	Kagoshima Prefecture, Amami Islands
[Ref.: #16047]	Country	Japan
[Ref.: #16047]	Country ISO 3 Code	JPN
[Ref.: #16047]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Mangrove
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB471559 (>99% sequence identity) for Demequina from Microbeatlas

Aquatic Samples	18
Soil Samples	3
Animal Samples	1
Plant Samples	2
Total Samples	24

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16047]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16047]	Demequina salsinemoris gene for 16S rRNA, partial sequence, strain: KV-810	AB471559	1436	ENA	577470 tax ID
[Ref.: #20218]	Demequina salsinemoris gene for 16S rRNA, partial sequence	AB639017	1478	ENA	577470 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Demequina salsinemoris NBRC 105323	GCA_000974805	scaffold	GenBank	577470 tax ID	*
[Ref.: #66792]	Demequina salsinemoris NBRC 105323	2671180261	draft	JGI IMG/M	577470 tax ID	*

[Ref.: #16047]

GC-content

70-72 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Demequina salsinemoris NBRC 105323 NCBI: GCA_000974805.1
[Ref.: #69481]	spore-forming	no	91	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Demequina salsinemoris NBRC 105323 NCBI: GCA_000974805.1
[Ref.: #69480]	motile	yes	66.332	no
[Ref.: #69480]	flagellated	no	90.507	no
[Ref.: #69480]	gram-positive	yes	89.96	no
[Ref.: #69480]	anaerobic	no	98.115	yes
[Ref.: #69480]	aerobic	no	65.728	no
[Ref.: #69480]	halophile	no	76.881	yes
[Ref.: #69480]	spore-forming	no	90.944	no
[Ref.: #69480]	glucose-util	yes	86.082	yes
[Ref.: #69480]	thermophile	no	98.084	yes
[Ref.: #69480]	glucose-ferment	no	51.758	yes

Availability in culture collections External links

[Ref.: #16047]

Culture collection no.

DSM 22060, NBRC 105323

[Ref.: #86830]

SI-ID 406075

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Demequina salsinemoris sp. nov., isolated on agar media supplemented with ascorbic acid or rutin.	Matsumoto A, Nakai K, Morisaki K, Omura S, Takahashi Y	Int J Syst Evol Microbiol	10.1099/ijs.0.012617-0	2009	*

References

#16047

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22060

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#21365

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#29412

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25819 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86830

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406075.1 )

#25819

IJSEM 1206 2010 ( DOI 10.1099/ijs.0.012617-0 , PubMed 19667388 )

* These data were automatically processed and therefore are not curated

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Demequina salsinemoris NBRC 105323

Demequina salsinemoris NBRC 105323

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