Name: Alkaliflexus imshenetskii Z-7010
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17805

Type strain:

Species: Alkaliflexus imshenetskii

Strain Designation: Z-7010

Culture col. no.: DSM 15055, VKM B-2311

Strain history: <- T. N. Zhilina, INMI; Z-7010. The 16S rRNA gene sequence determined in the DSMZ is FR839023, which is not identical to the original nucleotide accession AJ784993

NCBI tax ID(s): 927658 (strain), 286730 (species)

SI-ID 401947

DSM 15055

Other strains from Alkaliflexus imshenetskii

A. imshenetskii Z-7015-1, DSM 15056
A. imshenetskii Z-7015-2, DSM 15057
A. imshenetskii Z-7021, DSM 15058
A. imshenetskii Z-7022, DSM 15059

Section

Alkaliflexus imshenetskii Z-7010 is a microaerotolerant, mesophilic, Gram-negative bacterium that was isolated from as a component of an anaerobic cellulose-degrading enrichment culture originating from the alkaline Lake Verkhnee Beloye.

Gram-negative
motile
rod-shaped
mesophilic
microaerotolerant
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Marinilabiliaceae*
	Genus	*Alkaliflexus*
	Species	**Alkaliflexus imshenetskii**
	Full Scientific Name (LPSN)	Alkaliflexus imshenetskii Zhilina et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #43353]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %100

[Ref.: #43353]	Cell length	4-10 µm

[Ref.: #43353]	Cell width	0.25-0.4 µm

[Ref.: #43353]	Cell shape	rod-shaped

[Ref.: #43353]	Motility	yes

[Ref.: #43353]	Flagellum arrangement	gliding

[Ref.: #43353]

Colony color

pink

[Ref.: #43353]	Pigment production	yes
[Ref.: #43353]	Pigment name	Carotenoid(s)

Multimedia content

[Ref.: #66793]

Caption:

electron microscopic image

[Ref.: #66793]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5687]

Culture medium

ALKALIFLEXUS MEDIUM (DSMZ Medium 1175)

[Ref.: #5687]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #5687]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #5687]			growth	30 °C

[Ref.: #5687]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43353]			growth	7.2-10.2
[Ref.: #43353]			growth	8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43353]	Oxygen tolerance	microaerotolerant
[Ref.: #43353]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.998

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.929

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43353]		NaCl		growth	0.8-53	g/L
[Ref.: #43353]		NaCl		optimum	20	g/L

[Ref.: #43353]

Observation

desiccation survivability

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43353]	agarose ChEBI	-	growth
[Ref.: #43353]	alginate ChEBI	-	growth
[Ref.: #43353]	arabinose ChEBI	+	anaerobic growth
[Ref.: #43353]	casamino acids	-	growth
[Ref.: #43353]	cellobiose ChEBI	+	anaerobic growth
[Ref.: #43353]	cellulose ChEBI	-	growth
[Ref.: #43353]	cellulose ChEBI	-	degradation
[Ref.: #43353]	chitin ChEBI	-	growth
[Ref.: #43353]	chitosan ChEBI	-	growth
[Ref.: #43353]	dextran ChEBI	+	anaerobic growth
[Ref.: #43353]	dextrin ChEBI	+	anaerobic growth
[Ref.: #43353]	ethanol ChEBI	-	growth
[Ref.: #43353]	fructose ChEBI	-	growth
[Ref.: #43353]	fumarate ChEBI	-	growth
[Ref.: #43353]	galactose ChEBI	+	anaerobic growth
[Ref.: #43353]	gelatin ChEBI	-	growth
[Ref.: #43353]	glucose ChEBI	+	anaerobic growth
[Ref.: #43353]	glycerol ChEBI	-	growth
[Ref.: #43353]	glycogen ChEBI	+	anaerobic growth
[Ref.: #43353]	lactose ChEBI	-	growth
[Ref.: #43353]	malate ChEBI	-	growth
[Ref.: #43353]	maltose ChEBI	+	anaerobic growth
[Ref.: #43353]	mannitol ChEBI	-	growth
[Ref.: #43353]	mannose ChEBI	+	anaerobic growth
[Ref.: #43353]	methanol ChEBI	-	growth
[Ref.: #43353]	myo-inositol ChEBI	-	growth
[Ref.: #43353]	N-acetylglucosamine ChEBI	+	anaerobic growth
[Ref.: #43353]	pectin ChEBI	+	anaerobic growth
[Ref.: #43353]	peptone	-	growth
[Ref.: #43353]	pullulan ChEBI	+	anaerobic growth
[Ref.: #43353]	pyruvate ChEBI	-	growth
[Ref.: #43353]	ribose ChEBI	-	growth
[Ref.: #43353]	starch ChEBI	+	anaerobic growth
[Ref.: #43353]	succinate ChEBI	-	growth
[Ref.: #43353]	sucrose ChEBI	+	anaerobic growth
[Ref.: #43353]	trehalose ChEBI	+	anaerobic growth
[Ref.: #43353]	xanthan ChEBI	-	growth
[Ref.: #43353]	xylan ChEBI	+	anaerobic growth
[Ref.: #43353]	xylose ChEBI	+	anaerobic growth
[Ref.: #43353]	yeast extract (0.01 %, w/v)	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43353]	acetate ChEBI	yes
[Ref.: #43353]	dihydrogen ChEBI	yes
[Ref.: #43353]	formate ChEBI	yes
[Ref.: #43353]	fumarate ChEBI	yes
[Ref.: #43353]	propionate ChEBI	yes
[Ref.: #43353]	succinate ChEBI	yes

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43353]		catalase	+	1.11.1.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5687]	Sample type/isolated from	as a component of an anaerobic cellulose-degrading enrichment culture originating from the alkaline Lake Verkhnee Beloye
[Ref.: #5687]	Geographic location (country and/or sea, region)	Central Asia, South-Eastern Transbaikal region
[Ref.: #5687]	Country	Russia
[Ref.: #5687]	Country ISO 3 Code	RUS
[Ref.: #5687]	Continent	Asia

[Ref.: #43353]	Sample type/isolated from	cellulose degrading culture from Lake Verkhnee Beloye
[Ref.: #43353]	Geographic location (country and/or sea, region)	South-Eastern Transbaikal Region
[Ref.: #43353]	Country	Russia
[Ref.: #43353]	Country ISO 3 Code	RUS
[Ref.: #43353]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Laboratory	#Lab enrichment
#Condition	#Alkaline	-
#Condition	#Anoxic (anaerobic)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FR839023 (>99% sequence identity) for Alkaliflexus imshenetskii subclade from Microbeatlas

Aquatic Samples	213
Soil Samples	105
Animal Samples	2619
Plant Samples	70
Total Samples	3007

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5687]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5687]	Alkaliflexus imshenetskii partial 16S rRNA gene, type strain DSM 15055T, isolate Z-7010	FR839023	1515	ENA	286730 tax ID
[Ref.: #5687]	Alkaliflexus imshenetskii 16S rRNA gene, type strain Z-7010	AJ784993	1467	ENA	927658 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Alkaliflexus imshenetskii DSM 15055 Z-7010, DSM 15055	GCA_000517065	scaffold	GenBank	927658 tax ID	*
[Ref.: #66792]	Alkaliflexus imshenetskii DSM 15055	927658.4	wgs	PATRIC	927658 tax ID	*
[Ref.: #66792]	Alkaliflexus imshenetskii Z-7010, DSM 15055	2509276023	draft	JGI IMG/M	927658 tax ID	*

[Ref.: #43353]	GC-content	44.3 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #5687]	GC-content	42.7 mol%	sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Alkaliflexus imshenetskii DSM 15055 Z-7010 NCBI: GCA_000517065.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Alkaliflexus imshenetskii DSM 15055 Z-7010 NCBI: GCA_000517065.1
[Ref.: #69480]	motile	no	81.309	no
[Ref.: #69480]	flagellated	no	96.001	yes
[Ref.: #69480]	gram-positive	no	96.088	no
[Ref.: #69480]	anaerobic	yes	91.802	no
[Ref.: #69480]	halophile	no	81.494	no
[Ref.: #69480]	spore-forming	no	93.635	no
[Ref.: #69480]	glucose-util	yes	91.775	no
[Ref.: #69480]	aerobic	no	93.236	no
[Ref.: #69480]	thermophile	no	96.488	yes
[Ref.: #69480]	glucose-ferment	yes	54.874	no

Availability in culture collections External links

[Ref.: #5687]

Culture collection no.

DSM 15055, VKM B-2311

[Ref.: #86797]

SI-ID 401947

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Alkalitalea saponilacus gen. nov., sp. nov., an obligately anaerobic, alkaliphilic, xylanolytic bacterium from a meromictic soda lake.	Zhao B, Chen S	Int J Syst Evol Microbiol	10.1099/ijs.0.038315-0	2011	*
Phylogeny	Geofilum rubicundum gen. nov., sp. nov., isolated from deep subseafloor sediment.	Miyazaki M, Koide O, Kobayashi T, Mori K, Shimamura S, Nunoura T, Imachi H, Inagaki F, Nagahama T, Nogi Y, Deguchi S, Takai K	Int J Syst Evol Microbiol	10.1099/ijs.0.032326-0	2011	*
Phylogeny	Alkaliflexus imshenetskii gen. nov. sp. nov., a new alkaliphilic gliding carbohydrate-fermenting bacterium with propionate formation from a soda lake.	Zhilina TN, Appel R, Probian C, Brossa EL, Harder J, Widdel F, Zavarzin GA	Arch Microbiol	10.1007/s00203-004-0722-0	2004	*

References

#5687

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15055

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43353

Tatyana N. Zhilina, Ramona Appel, Christina Probian, Enrique Llobet Brossa, Jens Harder, Friedrich Widdel, Georgii A. Zavarzin: Alkaliflexus imshenetskii gen. nov. sp. nov., a new alkaliphilic gliding carbohydrate-fermenting bacterium with propionate formation from a soda lake. Arch Microbiol 182: 244 - 253 2004 ( DOI 10.1007/s00203-004-0722-0 , PubMed 15340778 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86797

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401947.1 )

* These data were automatically processed and therefore are not curated

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Alkaliflexus imshenetskii DSM 15055 Z-7010

Alkaliflexus imshenetskii DSM 15055 Z-7010

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