Ruania albidiflava 3-6 | Type strain | DSM 18029, CGMCC 4.3142, JCM 13910, PCM 2644, NBRC 106322, CIP 109565

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electron microscopic image

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electron microscopic image

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Information on culture and growth conditions Culture and growth conditions

Culture medium

RICH MEDIUM (DSMZ Medium 736)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

ISP 2

Culture medium growth

Culture medium composition

Culture medium

ISP 4

Culture medium growth

Culture medium composition

[Ref.: #36057]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #36057]

Culture medium growth

[Ref.: #36057]

Culture medium composition

Culture medium

CIP Medium 72

Culture medium growth

Medium recipe provided by DSMZ

Culture medium link

Culture medium

CIP Medium 112

Culture medium growth

Medium recipe provided by DSMZ

Culture medium link

		Temperature
[Ref.: #7254]	growth	28 °C
[Ref.: #31908]	growth	20-37 °C
[Ref.: #31908]	optimum	28 °C
[Ref.: #36057]	growth	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #7254]	Temperature range	mesophilic
[Ref.: #31908]	Temperature range	mesophilic
[Ref.: #36057]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31908]			optimum	6.5-10.5
[Ref.: #31908]			growth	5.5-12.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31908]	Oxygen tolerance	aerobe
[Ref.: #116186]	Oxygen tolerance	facultative anaerobe

[Ref.: #31908]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.987

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31908]		NaCl		growth	0-10	%
[Ref.: #31908]		NaCl		optimum	5	%

[Ref.: #67770]

Observation

quinones: MK-8(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31908]	acetate ChEBI	+	carbon source
[Ref.: #31908]	alanine ChEBI	+	carbon source
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31908]	arginine ChEBI	+	carbon source
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31908]	fructose ChEBI	+	carbon source
[Ref.: #31908]	fucose ChEBI	+	carbon source
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31908]	glucose ChEBI	+	carbon source
[Ref.: #31908]	glutamate ChEBI	+	carbon source
[Ref.: #31908]	glycerol ChEBI	+	carbon source
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31908]	lactose ChEBI	+	carbon source
[Ref.: #31908]	maltose ChEBI	+	carbon source
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31908]	methyl D-glucoside ChEBI	+	carbon source
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31908]	nitrate ChEBI	+	reduction
[Ref.: #116186]	nitrate ChEBI	+	reduction
[Ref.: #116186]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31908]	proline ChEBI	+	carbon source
[Ref.: #31908]	ribose ChEBI	+	carbon source
[Ref.: #31908]	sorbitol ChEBI	+	carbon source
[Ref.: #31908]	sucrose ChEBI	+	carbon source
[Ref.: #31908]	trehalose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #116186]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	+	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31908]	catalase	+	1.11.1.6
[Ref.: #116186]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116186]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #116186]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #116186]	11597	not determinedn.d.	+/-	-	+/-	+/-	+/-	+/-	-	+/-	+/-	+/-	+/-	+/-	+/-	-	+/-	-	-	-	-	-	-	+/-	-	-	+/-	+/-	+/-	+/-	+/-	+/-	+/-	+/-	-	-	+/-	+/-	+/-	+/-	+/-	+/-	+/-	-	-	+/-	+/-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116186]	11959	-	+	+	+	-	+	+	+	+	-	+	+	+	+	-	+	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7254]	Sample type/isolated from	soil, farmland
[Ref.: #7254]	Geographic location (country and/or sea, region)	Shandong Province
[Ref.: #7254]	Country	China
[Ref.: #7254]	Country ISO 3 Code	CHN
[Ref.: #7254]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Farmland soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Shandong Province
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #116186]	Sample type/isolated from	Environment, Soil, farmland
[Ref.: #116186]	Geographic location (country and/or sea, region)	Shandong
[Ref.: #116186]	Country	China
[Ref.: #116186]	Country ISO 3 Code	CHN
[Ref.: #116186]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	-
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ343153 (>99% sequence identity) for Ruania albidiflava subclade from Microbeatlas

Aquatic Samples	113
Soil Samples	409
Animal Samples	842
Plant Samples	99
Total Samples	1463

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7254]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116186]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7254]	Ruania albidiflava strain AS 4.3142 16S ribosomal RNA gene, partial sequence	DQ343153	1454	ENA	366586 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Ruania albidiflava DSM 18029	GCA_000421225	contig	GenBank	1123065 tax ID
[Ref.: #66792]	Ruania albidiflava DSM 18029	1123065.3	wgs	PATRIC	1123065 tax ID	*
[Ref.: #66792]	Ruania albidiflava DSM 18029	2524614511	draft	JGI IMG/M	1123065 tax ID	*

[Ref.: #7254]	GC-content	69.8 mol%
[Ref.: #67770]	GC-content	69.8 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Ruania albidiflava DSM 18029 PATRIC: 1123065.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Ruania albidiflava DSM 18029 PATRIC: 1123065.3
[Ref.: #69480]	motile	no	90.74	yes
[Ref.: #69480]	flagellated	no	98.714	yes
[Ref.: #69480]	gram-positive	yes	87.459	yes
[Ref.: #69480]	anaerobic	no	99.109	yes
[Ref.: #69480]	aerobic	yes	85.471	no
[Ref.: #69480]	halophile	no	79.645	no
[Ref.: #69480]	spore-forming	no	89.131	yes
[Ref.: #69480]	thermophile	no	93.783	yes
[Ref.: #69480]	glucose-util	yes	85.718	yes
[Ref.: #69480]	glucose-ferment	no	80.768	no

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