Acidilobus saccharovorans (DSM 16705, JCM 18335, VKM B-2471)

Name: Acidilobus saccharovorans (DSM 16705, JCM 18335, VKM B-2471)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17800

Type strain

Strain Designation 345-15

Culture col. no. DSM 16705 JCM 18335 VKM B-2471 345-15

NCBI tax ID(s) 242703

Special Collections DSMZ JCM

History <- M. Prokofeva, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; 345-15 <- M. Prokofeva; {1999} DSM 16705 <-- M. Prokofeva 345-15.

Links

Acidilobus saccharovorans 345-15 is an anaerobe archaeon that was isolated from mixture of water and mud from an acidic terrestrial hot pool.

anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 405657

JCM 18335,DSM 16705

@ref 20215

Last LPSN update 2026-02-09 11:22:18

Domain Archaea

Phylum Thermoproteota

Class Thermoprotei

Order Acidilobales

Family Acidilobaceae

Genus Acidilobus

Species Acidilobus saccharovorans

Full scientific name Acidilobus saccharovorans Prokofeva et al. 2009

Morphology

Colony morphology

6576

Incubation period3-7 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6576	ACIDOLOBUS ACETICUS MEDIUM (DSMZ Medium 901)		Medium recipe at MediaDive	Name: ACIDOLOBUS ACETICUS MEDIUM (DSMZ Medium 901; with strain-specific modifications) Composition: Sulfur 9.83284 g/l D-Glucose 1.96657 g/l Na2S x 9 H2O 0.442478 g/l CaCl2 x 2 H2O 0.324484 g/l MgCl2 x 6 H2O 0.324484 g/l KH2PO4 0.324484 g/l NH4Cl 0.324484 g/l KCl 0.324484 g/l Yeast extract 0.0983284 g/l HCl 0.00245821 g/l FeCl2 x 4 H2O 0.00147493 g/l Sodium resazurin 0.000491642 g/l CoCl2 x 6 H2O 0.000186824 g/l Pyridoxine hydrochloride 9.83284e-05 g/l MnCl2 x 4 H2O 9.83284e-05 g/l ZnCl2 6.88299e-05 g/l p-Aminobenzoic acid 4.91642e-05 g/l Calcium D-(+)-pantothenate 4.91642e-05 g/l Nicotinic acid 4.91642e-05 g/l Riboflavin 4.91642e-05 g/l (DL)-alpha-Lipoic acid 4.91642e-05 g/l Thiamine HCl 4.91642e-05 g/l Na2MoO4 x 2 H2O 3.53982e-05 g/l NiCl2 x 6 H2O 2.35988e-05 g/l Biotin 1.96657e-05 g/l Folic acid 1.96657e-05 g/l H3BO3 5.89971e-06 g/l CuCl2 x 2 H2O 1.96657e-06 g/l Vitamin B12 9.83284e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
6576	positive	growth	80
67770	positive	growth	80

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
6576	anaerobe
125439	anaerobe	99.1

Spore formation

@ref	Spore formation	Confidence
125438		91.532

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	-
#Environmental	#Terrestrial	#Mud (Sludge)
#Condition	#Acidic	-
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
6576	mixture of water and mud from an acidic terrestrial hot pool	Kamchatka Peninsula, Orange Thermal Field, 54° 30' 40'' N 160° 00' 06'' E	Russia	RUS	Asia	54.5111	160.002 54.5111/160.002
67770	Hot acidic pool in the Orange Thermal Field of Uzon Caldera	Kamchatka	Russia	RUS

Taxonmaps

69479

Global distribution of 16S sequence AY350586 (>99% sequence identity) for Acidilobus from Microbeatlas

Aquatic Samples	5
Soil Samples
Animal Samples
Plant Samples
Total Samples	5

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6576	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM14491v1 assembly for Acidilobus saccharovorans 345-15	complete	GCA_000144915	666510.18	648028003	666510	99.19

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6576	Acidilobus saccharovorans strain 345-15 16S ribosomal RNA gene, partial sequence	AY350586	1451		666510

GC content

@ref	GC-content (mol%)	Method
6576	54.5	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Acidilobus saccharovorans 345-15
IMG: 648028003

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	65.30	no
125439	motility	BacteriaNetⓘ	no	52.80	no
125439	gram_stain	BacteriaNetⓘ	positive	61.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Acidilobus saccharovorans 345-15 345-15
NCBI: GCA_000144915.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	75.16	no
125438	anaerobic	anaerobicⓘ	yes	80.70	yes
125438	aerobic	aerobicⓘ	no	87.30	yes
125438	spore-forming	spore-formingⓘ	no	91.53	no
125438	thermophilic	thermophileⓘ	yes	83.23	yes
125438	flagellated	motile2+ⓘ	no	88.38	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	A Novel Highly Thermostable Multifunctional Beta-Glycosidase from Crenarchaeon Acidilobus saccharovorans.	Gumerov VM, Rakitin AL, Mardanov AV, Ravin NV.	Archaea	10.1155/2015/978632	2015
Phylogeny	The genome sequence of the crenarchaeon Acidilobus saccharovorans supports a new order, Acidilobales, and suggests an important ecological role in terrestrial acidic hot springs.	Mardanov AV, Svetlitchnyi VA, Beletsky AV, Prokofeva MI, Bonch-Osmolovskaya EA, Ravin NV, Skryabin KG.	Appl Environ Microbiol	10.1128/aem.00599-10	2010
Genetics	Genomics-informed isolation and characterization of a symbiotic Nanoarchaeota system from a terrestrial geothermal environment.	Wurch L, Giannone RJ, Belisle BS, Swift C, Utturkar S, Hettich RL, Reysenbach AL, Podar M.	Nat Commun	10.1038/ncomms12115	2016
	Tris inhibits a GH1 beta-glucosidase by a linear mixed inhibition mechanism.	Chagas RS, Marana SR.	PLoS One	10.1371/journal.pone.0320120	2025
	Microreactor equipped with naturally acid-resistant histidine ammonia lyase from an extremophile.	Ade C, Marcelino TF, Dulchavsky M, Wu K, Bardwell JCA, Stadler B.	Mater Adv	10.1039/d2ma00051b	2022
Genetics	Microbial diversity analysis of Chumathang geothermal spring, Ladakh, India.	Anu K, Kumari S, Choudhary G, Devi S.	Braz J Microbiol	10.1007/s42770-024-01284-3	2024
Genetics	Metagenomics insight into Puga geothermal geyser located in Himalayan Geothermal Belt (Trans-Himalayan Plateau) Ladakh, India.	Kumari S, Choudhary G, Anu K, Devi S.	Braz J Microbiol	10.1007/s42770-024-01408-9	2024
Metabolism	Nicotinamidase from the thermophilic archaeon Acidilobus saccharovorans: structural and functional characteristics.	Stekhanova TN, Bezsudnova EY, Mardanov AV, Osipov EM, Ravin NV, Skryabin KG, Popov VO.	Biochemistry (Mosc)	10.1134/s0006297914010088	2014
	Characterization of a Novel Hyperthermophilic GH1 beta-Glucosidase from Acidilobus sp. and Its Application in the Hydrolysis of Soybean Isoflavone Glycosides.	He J, Li Y, Sun X, Zuo D, Wang M, Zheng X, Yu P, Shi P.	Microorganisms	10.3390/microorganisms12030533	2024
Enzymology	Iron-dependent superoxide dismutase from novel thermoacidophilic crenarchaeon Acidilobus saccharovorans: from gene to active enzyme.	Slutskaya ES, Bezsudnova EY, Mardanov AV, Safenkova IV, Kleimenov SY, Chebotareva NA, Gumerov VM, Ravin NV, Skryabin KG, Popov VO.	Biochemistry (Mosc)	10.1134/s0006297912120048	2012
Phylogeny	Moderately thermostable GH1 beta-glucosidases from hyperacidophilic archaeon Cuniculiplasma divulgatum S5.	Khusnutdinova AN, Tran H, Devlekar S, Distaso MA, Kublanov IV, Skarina T, Stogios P, Savchenko A, Ferrer M, Golyshina OV, Yakunin AF, Golyshin PN.	FEMS Microbiol Ecol	10.1093/femsec/fiae114	2024
Enzymology	Biochemical characterization of a new nicotinamidase from an unclassified bacterium thriving in a geothermal water stream microbial mat community.	Zapata-Perez R, Martinez-Monino AB, Garcia-Saura AG, Cabanes J, Takami H, Sanchez-Ferrer A.	PLoS One	10.1371/journal.pone.0181561	2017
	ExtRamp: a novel algorithm for extracting the ramp sequence based on the tRNA adaptation index or relative codon adaptiveness.	Miller JB, Brase LR, Ridge PG.	Nucleic Acids Res	10.1093/nar/gky1193	2019
Transcriptome	On the existence of wavelet symmetries in archaea DNA.	Cattani C.	Comput Math Methods Med	10.1155/2012/673934	2012
	Thermoacidophilic Alicyclobacillus Superoxide Dismutase: Good Candidate as Additives in Food and Medicine.	Dong X, Wang W, Li S, Han H, Lv P, Yang C.	Front Microbiol	10.3389/fmicb.2021.577001	2021
	Cage and maternal effects on the bacterial communities of the murine gut.	Singh G, Brass A, Cruickshank SM, Knight CG.	Sci Rep	10.1038/s41598-021-89185-5	2021
Metabolism	Predominant Acidilobus-like populations from geothermal environments in yellowstone national park exhibit similar metabolic potential in different hypoxic microbial communities.	Jay ZJ, Rusch DB, Tringe SG, Bailey C, Jennings RM, Inskeep WP.	Appl Environ Microbiol	10.1128/aem.02860-13	2014
	An Archaeal Chitinase With a Secondary Capacity for Catalyzing Cellulose and Its Biotechnological Applications in Shell and Straw Degradation.	Chen L, Wei Y, Shi M, Li Z, Zhang SH.	Front Microbiol	10.3389/fmicb.2019.01253	2019
Metabolism	Identification and characterization of a bacterial glutamic peptidase.	Jensen K, Ostergaard PR, Wilting R, Lassen SF.	BMC Biochem	10.1186/1471-2091-11-47	2010
	Functionalized Membrane Domains: An Ancestral Feature of Archaea?	Tourte M, Schaeffer P, Grossi V, Oger PM.	Front Microbiol	10.3389/fmicb.2020.00526	2020
Genetics	Omics on bioleaching: current and future impacts.	Martinez P, Vera M, Bobadilla-Fazzini RA.	Appl Microbiol Biotechnol	10.1007/s00253-015-6903-8	2015
	Tracing back variations in archaeal ESCRT-based cell division to protein domain architectures.	Frohn BP, Hartel T, Cox J, Schwille P.	PLoS One	10.1371/journal.pone.0266395	2022
Metabolism	A Key Enzyme of the NAD⁺ Salvage Pathway in Thermus thermophilus: Characterization of Nicotinamidase and the Impact of Its Gene Deletion at High Temperatures.	Taniguchi H, Sungwallek S, Chotchuang P, Okano K, Honda K.	J Bacteriol	10.1128/jb.00359-17	2017
Metabolism	Involvement of intermediate sulfur species in biological reduction of elemental sulfur under acidic, hydrothermal conditions.	Boyd ES, Druschel GK.	Appl Environ Microbiol	10.1128/aem.03160-12	2013
	Activity-Based Protein Profiling for the Identification of Novel Carbohydrate-Active Enzymes Involved in Xylan Degradation in the Hyperthermophilic Euryarchaeon Thermococcus sp. Strain 2319x1E.	Klaus T, Ninck S, Albersmeier A, Busche T, Wibberg D, Jiang J, Elcheninov AG, Zayulina KS, Kaschani F, Brasen C, Overkleeft HS, Kalinowski J, Kublanov IV, Kaiser M, Siebers B.	Front Microbiol	10.3389/fmicb.2021.734039	2021
Metabolism	A Novel Transcriptional Regulator Related to Thiamine Phosphate Synthase Controls Thiamine Metabolism Genes in Archaea.	Rodionov DA, Leyn SA, Li X, Rodionova IA.	J Bacteriol	10.1128/jb.00743-16	2017
	Enhancing the Resolution of Rumen Microbial Classification from Metatranscriptomic Data Using Kraken and Mothur.	Neves ALA, Li F, Ghoshal B, McAllister T, Guan LL.	Front Microbiol	10.3389/fmicb.2017.02445	2017
	Factors controlling the distribution of archaeal tetraethers in terrestrial hot springs.	Pearson A, Pi Y, Zhao W, Li W, Li Y, Inskeep W, Perevalova A, Romanek C, Li S, Zhang CL.	Appl Environ Microbiol	10.1128/aem.02450-07	2008
	The Proteome and Lipidome of Thermococcus kodakarensis across the Stationary Phase.	Gagen EJ, Yoshinaga MY, Garcia Prado F, Hinrichs KU, Thomm M.	Archaea	10.1155/2016/5938289	2016
Genetics	Phylogenomics of prokaryotic ribosomal proteins.	Yutin N, Puigbo P, Koonin EV, Wolf YI.	PLoS One	10.1371/journal.pone.0036972	2012
Enzymology	Bioprospecting of novel thermostable beta-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis.	Tiwari R, Singh PK, Singh S, Nain PKS, Nain L, Shukla P.	Biotechnol Biofuels	10.1186/s13068-017-0932-8	2017
Metabolism	The Geoglobus acetivorans genome: Fe(III) reduction, acetate utilization, autotrophic growth, and degradation of aromatic compounds in a hyperthermophilic archaeon.	Mardanov AV, Slododkina GB, Slobodkin AI, Beletsky AV, Gavrilov SN, Kublanov IV, Bonch-Osmolovskaya EA, Skryabin KG, Ravin NV.	Appl Environ Microbiol	10.1128/aem.02705-14	2015
Metabolism	Molecular phylogeny and intricate evolutionary history of the three isofunctional enzymes involved in the oxidation of protoporphyrinogen IX.	Kobayashi K, Masuda T, Tajima N, Wada H, Sato N.	Genome Biol Evol	10.1093/gbe/evu170	2014
Metabolism	Structural basis of thermal stability of the tungsten cofactor synthesis protein MoaB from Pyrococcus furiosus.	Havarushka N, Fischer-Schrader K, Lamkemeyer T, Schwarz G.	PLoS One	10.1371/journal.pone.0086030	2014
	Isolation and Characterization of the First Xylanolytic Hyperthermophilic Euryarchaeon Thermococcus sp. Strain 2319x1 and Its Unusual Multidomain Glycosidase.	Gavrilov SN, Stracke C, Jensen K, Menzel P, Kallnik V, Slesarev A, Sokolova T, Zayulina K, Brasen C, Bonch-Osmolovskaya EA, Peng X, Kublanov IV, Siebers B.	Front Microbiol	10.3389/fmicb.2016.00552	2016
	The first prokaryotic trehalose synthase complex identified in the hyperthermophilic crenarchaeon Thermoproteus tenax.	Zaparty M, Hagemann A, Brasen C, Hensel R, Lupas AN, Brinkmann H, Siebers B.	PLoS One	10.1371/journal.pone.0061354	2013
Genetics	CRISPR loci reveal networks of gene exchange in archaea.	Brodt A, Lurie-Weinberger MN, Gophna U.	Biol Direct	10.1186/1745-6150-6-65	2011
	TPV1, the first virus isolated from the hyperthermophilic genus Thermococcus.	Gorlas A, Koonin EV, Bienvenu N, Prieur D, Geslin C.	Environ Microbiol	10.1111/j.1462-2920.2011.02662.x	2012
Phylogeny	Phylogenetic and Functional Analysis of Metagenome Sequence from High-Temperature Archaeal Habitats Demonstrate Linkages between Metabolic Potential and Geochemistry.	Inskeep WP, Jay ZJ, Herrgard MJ, Kozubal MA, Rusch DB, Tringe SG, Macur RE, Jennings Rd, Boyd ES, Spear JR, Roberto FF.	Front Microbiol	10.3389/fmicb.2013.00095	2013
Phylogeny	Isolation of the anaerobic thermoacidophilic crenarchaeote Acidilobus saccharovorans sp. nov. and proposal of Acidilobales ord. nov., including Acidilobaceae fam. nov. and Caldisphaeraceae fam. nov.	Prokofeva MI, Kostrikina NA, Kolganova TV, Tourova TP, Lysenko AM, Lebedinsky AV, Bonch-Osmolovskaya EA	Int J Syst Evol Microbiol	10.1099/ijs.0.010355-0	2009

References

#6576	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16705
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Acidilobus saccharovorans (DSM 16705, JCM 18335, VKM B-2471)

Name and taxonomic classification

Morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000144915

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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