Name: Acetomicrobium thermoterrenum RWcit2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17778

Type strain:

Species: Acetomicrobium thermoterrenum

Strain Designation: RWcit2

Culture col. no.: DSM 13490, ACM 5076

Strain history: <- R. J. Menes, Universidad de la República, Montevideo, Uruguay; ACM 5076 <- ACM <- G. Rees, University of Canberra, Belconnen, Australian Capital Territory, Australia; RWcit2

NCBI tax ID(s): 1120987 (strain), 1120986 (species)

SI-ID 49477

DSM 13490, ACM 5076

Section

Acetomicrobium thermoterrenum RWcit2 is an anaerobe, chemoorganotroph, thermophilic bacterium that was isolated from production fluid from oil field.

Gram-negative
motile
rod-shaped
anaerobe
chemoorganotroph
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Synergistota*
	Class	*Synergistia*
	Order	*Synergistales*
	Family	*Synergistaceae*
	Genus	*Acetomicrobium*
	Species	**Acetomicrobium thermoterrenum**
	Full Scientific Name (LPSN)	Acetomicrobium thermoterrenum (Rees et al. 1997) Ben Hania et al. 2016

Synonym

Anaerobaculum thermoterrenum

Information on morphological and physiological properties Morphology

[Ref.: #43698]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.835

[Ref.: #43698]	Cell shape	rod-shaped

[Ref.: #43698]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5065]

Culture medium

ANAEROBACULUM MEDIUM (DSMZ Medium 104a)

[Ref.: #5065]

Culture medium growth

[Ref.: #5065]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5065]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #5065]	growth	50 °C
[Ref.: #43698]	growth	28-60 °C
[Ref.: #43698]	optimum	55 °C

[Ref.: #5065]	Temperature range	thermophilic
[Ref.: #43698]	Temperature range	thermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %99.24

	pH	Kind of pH		pH
[Ref.: #43698]			optimum	7-7.6
[Ref.: #43698]			growth	5.5-8.6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5065]	Oxygen tolerance	anaerobe
[Ref.: #43698]	Oxygen tolerance	obligate anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.999

[Ref.: #43698]

Nutrition type

chemoorganotroph

[Ref.: #43698]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.99

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43698]		NaCl		growth	0-20	g/L
[Ref.: #43698]		NaCl		optimum	10	g/L

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43698]	D-fructose ChEBI	+	assimilation
[Ref.: #43698]	D-glucose ChEBI	+	assimilation
[Ref.: #43698]	glycerol ChEBI	+	assimilation
[Ref.: #43698]	L-tartrate ChEBI	+	assimilation
[Ref.: #43698]	maltose ChEBI	-	assimilation
[Ref.: #43698]	mannose ChEBI	+	assimilation
[Ref.: #43698]	pyruvate ChEBI	+	assimilation

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43698]		catalase	-	1.11.1.6
[Ref.: #43698]		cytochrome oxidase	-	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5065]	Sample type/isolated from	production fluid from oil field
[Ref.: #5065]	Geographic location (country and/or sea, region)	Utah, Redwash oil field
[Ref.: #5065]	Country	USA
[Ref.: #5065]	Country ISO 3 Code	USA
[Ref.: #5065]	Continent	North America

[Ref.: #43698]	Sample type/isolated from	Production water of a petroleum reservoir from the Redwash oilfield
[Ref.: #43698]	Geographic location (country and/or sea, region)	Utah
[Ref.: #43698]	Country	USA
[Ref.: #43698]	Country ISO 3 Code	USA
[Ref.: #43698]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Engineered product
#Engineered	#Built environment	#Container (Reservoir)
#Engineered	#Industrial	#Oil reservoir
#Engineered	#Waste	#Wastewater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence U50711 (>99% sequence identity) for Acetomicrobium from Microbeatlas

Aquatic Samples	199
Soil Samples	9
Animal Samples	38
Plant Samples	19
Total Samples	265

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5065]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5065]	Anaerobaculum thermoterrenum strain RWcit 16S ribsomal RNA gene, partial sequence	U50711	1526	ENA	1120986 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Acetomicrobium thermoterrenum DSM 13490	GCA_900107215	scaffold	GenBank	1120987 tax ID	*
[Ref.: #66792]	Anaerobaculum sp. S15	2544872.3	wgs	PATRIC	2529858 tax ID	*
[Ref.: #66792]	Anaerobaculum thermoterrenum DSM 13490	1120987.3	wgs	PATRIC	1120987 tax ID	*
[Ref.: #66792]	Acetomicrobium thermoterrenum DSM 13490	2599185146	draft	JGI IMG/M	1120987 tax ID	*

[Ref.: #5065]

GC-content

44 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Acetomicrobium thermoterrenum DSM 13490 DSM 13490 NCBI: GCA_900107215.1
[Ref.: #69481]	spore-forming	yes	87	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Acetomicrobium thermoterrenum DSM 13490 DSM 13490 NCBI: GCA_900107215.1
[Ref.: #69480]	motile	no	71.637	no
[Ref.: #69480]	glucose-ferment	no	53.983	no
[Ref.: #69480]	flagellated	no	98.266	no
[Ref.: #69480]	gram-positive	no	98.25	yes
[Ref.: #69480]	anaerobic	yes	99.472	yes
[Ref.: #69480]	aerobic	no	97.813	yes
[Ref.: #69480]	halophile	no	91.054	yes
[Ref.: #69480]	spore-forming	no	94.32	no
[Ref.: #69480]	thermophile	yes	100	yes
[Ref.: #69480]	glucose-util	yes	82.941	yes

Availability in culture collections External links

[Ref.: #5065]

Culture collection no.

DSM 13490, ACM 5076

[Ref.: #86769]

SI-ID 49477

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Reclassification of Anaerobaculum mobile, Anaerobaculum thermoterrenum, Anaerobaculum hydrogeniformans as Acetomicrobium mobile comb. nov., Acetomicrobium thermoterrenum comb. nov. and Acetomicrobium hydrogeniformans comb. nov., respectively, and emendation of the genus Acetomicrobium.	Hania WB, Bouanane-Darenfed A, Cayol JL, Ollivier B, Fardeau ML	Int J Syst Evol Microbiol	10.1099/ijsem.0.000910	2016	*
Phylogeny	Description of Anaerobaculum hydrogeniformans sp. nov., an anaerobe that produces hydrogen from glucose, and emended description of the genus Anaerobaculum.	Maune MW, Tanner RS	Int J Syst Evol Microbiol	10.1099/ijs.0.024349-0	2011	*
Phylogeny	Anaerobaculum mobile sp. nov., a novel anaerobic, moderately thermophilic, peptide-fermenting bacterium that uses crotonate as an electron acceptor, and emended description of the genus Anaerobaculum.	Menes RJ, Muxi L	Int J Syst Evol Microbiol	10.1099/00207713-52-1-157	2002	*
Metabolism	Anaerobaculum thermoterrenum gen. nov., sp. nov., a novel, thermophilic bacterium which ferments citrate.	Rees GN, Patel BK, Grassia GS, Sheehy AJ	Int J Syst Bacteriol	10.1099/00207713-47-1-150	1997	*

References

#5065

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13490

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43698

Wajdi Ben Hania, Amel Bouanane-Darenfed, Jean-Luc Cayol, Bernard Ollivier, Marie-Laure Fardeau: Reclassification of Anaerobaculum mobile, Anaerobaculum thermoterrenum, Anaerobaculum hydrogeniformans as Acetomicrobium mobile comb. nov., Acetomicrobium thermoterrenum comb. nov. and Acetomicrobium hydrogeniformans comb. nov., respectively, and emendation of the genus Acetomicrobium. IJSEM 66: 1506 - 1509 2016 ( DOI 10.1099/ijsem.0.000910 , PubMed 26791251 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86769

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49477.1 )

* These data were automatically processed and therefore are not curated

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Acetomicrobium thermoterrenum DSM 13490 DSM 13490

Acetomicrobium thermoterrenum DSM 13490 DSM 13490

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