Acetomicrobium mobile (DSM 13181, ATCC BAA-54, JCM 12221)

Name: Acetomicrobium mobile (DSM 13181, ATCC BAA-54, JCM 12221)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17777

Type strain

Strain Designation NGA

Culture col. no. DSM 13181 ATCC BAA-54 JCM 12221 NGA

NCBI tax ID(s) 891968 97477

Special Collections DSMZ JCM Literature strains

History <- R. J. Menes, Universidad de la República, Montevideo, Uruguay; NGA <- R. J. Menes {1998} DSM 13181 <-- R. J. Menes strain NGA.

Links

Acetomicrobium mobile NGA is an anaerobe, chemoorganotroph, Gram-negative bacterium that forms circular colonies and was isolated from anaerobic wool-scouring wastewater treatment lagoon.

Gram-negative motile rod-shaped colony-forming anaerobe chemoorganotroph genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 87745

ATCC BAA-54,DSM 13181,JCM 12221

@ref 20215

Last LPSN update 2026-02-09 11:22:18

Domain Bacteria

Phylum Synergistota

Class Synergistia

Order Synergistales

Family Synergistaceae

Genus Acetomicrobium

Species Acetomicrobium mobile

Full scientific name Acetomicrobium mobile (Menes and Muxí 2002) Ben Hania et al. 2016

Synonyms (1)

Anaerobaculum mobile

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
43422	negative	2-8 µm	0.5-1 µm	rod-shaped		monotrichous, polar
43698	negative			rod-shaped

Colony morphology

@ref	Colony size	Colony color	Colony shape	Medium used
43422	1.0-1.5 mm	white	circular	BCTC agar

Multimedia

@ref:	66793
multimedia content:	EM_DSM_13181_1.jpg
multimedia.multimedia content:	EM_DSM_13181_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
4960	PY + X MEDIUM (DSMZ Medium 104b)		Medium recipe at MediaDive	Name: PY + X MEDIUM (DSMZ Medium 104b; with strain-specific modifications) Composition: Yeast extract 10.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l D-Glucose 2.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water
43422	BCTC agar

Culture temp

@ref	Growth	Type	Temperature (°C)
4960	positive	growth	55
43422	positive	maximum	55-60
43422	positive	growth	35-65
43698	positive	growth	35-65
43698	positive	optimum	55-60
67770	positive	growth	55

Culture pH

@ref	Ability	Type	PH	PH range
43422	positive	maximum	6.6-7.3
43698	positive	optimum	6.6-7.3
43422	positive	growth	5.4-8.7	alkaliphile
43698	positive	growth	5.4-8.7

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
4960	anaerobe
43422	anaerobe
43698	obligate anaerobe
125438	anaerobe	92.095
125439	anaerobe	99

Nutrition type

@ref	Type
43422	chemoorganotroph
43698	chemoorganotroph

Spore formation

@ref	Spore formation
43422
43698

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43422	NaCl	positive	growth	0-1.5 %
43422	NaCl	positive	optimum	0.8 %
43698	NaCl	positive	growth	0-15 g/L
43698	NaCl	positive	optimum	0.08 g/L

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43422	30916 ChEBI	2-oxoglutarate	-	growth
43422	22599 ChEBI	arabinose	-	growth
43422	15903 ChEBI	beta-d-glucose	+	fermentation
43422	17968 ChEBI	butyrate	-	growth
43422	17057 ChEBI	cellobiose	-	growth
43422	62968 ChEBI	cellulose	-	growth
43422	62968 ChEBI	cellulose	-	degradation
43422	16947 ChEBI	citrate	-	growth
43698	15824 ChEBI	D-fructose	+	assimilation
43698	17634 ChEBI	D-glucose	+	assimilation
43422	23652 ChEBI	dextrin	-	growth
43422	28260 ChEBI	galactose	-	growth
43422	5291 ChEBI	gelatin	-	growth
43422	24265 ChEBI	gluconate	+	assimilation
43422	29987 ChEBI	glutamate	-	growth
43422	17754 ChEBI	glycerol	+	fermentation
43422	17754 ChEBI	glycerol	+	assimilation
43698	17754 ChEBI	glycerol	+	assimilation
43422	15443 ChEBI	inulin	-	growth
43422	16467 ChEBI	L-arginine	+	assimilation
43422	15603 ChEBI	L-leucine	+	assimilation
43422	15589 ChEBI	L-malate	+	assimilation
43422	17295 ChEBI	L-phenylalanine	+	assimilation
43698	30924 ChEBI	L-tartrate	+	assimilation
43422	24996 ChEBI	lactate	-	growth
43422	17716 ChEBI	lactose	-	growth
43422	25115 ChEBI	malate	+	fermentation
43422	15792 ChEBI	malonate	-	growth
43422	17306 ChEBI	maltose	-	growth
43698	17306 ChEBI	maltose	-	assimilation
43422	37684 ChEBI	mannose	-	growth
43698	37684 ChEBI	mannose	-	assimilation
43422	28053 ChEBI	melibiose	-	growth
43422	17268 ChEBI	myo-inositol	-	growth
43422	62969 ChEBI	polygalacturonic acid	-	growth
43422	15361 ChEBI	pyruvate	+	fermentation
43698	15361 ChEBI	pyruvate	+	assimilation
43422	16634 ChEBI	raffinose	-	growth
43422	26546 ChEBI	rhamnose	-	growth
43422	15963 ChEBI	ribitol	-	growth
43422	9215 ChEBI	spectinomycin	-	growth
43422	28017 ChEBI	starch	+	assimilation
43422	30031 ChEBI	succinate	-	growth
43422	17992 ChEBI	sucrose	-	growth
43422	30929 ChEBI	tartrate	+	fermentation
43422		tryptone	+	assimilation
43422	37166 ChEBI	xylan	-	growth
43422	18222 ChEBI	xylose	-	growth

Metabolite production

@ref	Chebi-ID	Metabolite	Production
43422	30089 ChEBI	acetate
43422	18276 ChEBI	dihydrogen

Enzymes

@ref	Value	Activity	Ec
43698	catalase	-	1.11.1.6
43698	cytochrome oxidase	-	1.9.3.1

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	gluconeogenesis	100	8 of 8
66794	sulfopterin metabolism	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	suberin monomers biosynthesis	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	CMP-KDO biosynthesis	100	4 of 4
66794	methylglyoxal degradation	100	5 of 5
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	palmitate biosynthesis	95.45	21 of 22
66794	vitamin B1 metabolism	92.31	12 of 13
66794	threonine metabolism	90	9 of 10
66794	lipid A biosynthesis	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	isoleucine metabolism	87.5	7 of 8
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	Entner Doudoroff pathway	80	8 of 10
66794	hydrogen production	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	lipoate biosynthesis	80	4 of 5
66794	factor 420 biosynthesis	80	4 of 5
66794	propionate fermentation	80	8 of 10
66794	d-mannose degradation	77.78	7 of 9
66794	valine metabolism	77.78	7 of 9
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	serine metabolism	77.78	7 of 9
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	glycolysis	76.47	13 of 17
66794	biotin biosynthesis	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	purine metabolism	73.4	69 of 94
66794	flavin biosynthesis	73.33	11 of 15
66794	NAD metabolism	72.22	13 of 18
66794	photosynthesis	71.43	10 of 14
66794	propanol degradation	71.43	5 of 7
66794	glutamate and glutamine metabolism	71.43	20 of 28
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	pyrimidine metabolism	71.11	32 of 45
66794	starch degradation	70	7 of 10
66794	phenylalanine metabolism	69.23	9 of 13
66794	urea cycle	69.23	9 of 13
66794	leucine metabolism	69.23	9 of 13
66794	histidine metabolism	68.97	20 of 29
66794	octane oxidation	66.67	2 of 3
66794	arginine metabolism	66.67	16 of 24
66794	methane metabolism	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	tetrahydrofolate metabolism	64.29	9 of 14
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	degradation of sugar alcohols	62.5	10 of 16
66794	lysine metabolism	61.9	26 of 42
66794	methanofuran biosynthesis	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	glycogen metabolism	60	3 of 5
66794	cellulose degradation	60	3 of 5
66794	alanine metabolism	58.62	17 of 29
66794	tryptophan metabolism	57.89	22 of 38
66794	oxidative phosphorylation	56.04	51 of 91
66794	vitamin B6 metabolism	54.55	6 of 11
66794	kanosamine biosynthesis II	50	1 of 2
66794	glycine metabolism	50	5 of 10
66794	adipate degradation	50	1 of 2
66794	myo-inositol biosynthesis	50	5 of 10
66794	pantothenate biosynthesis	50	3 of 6
66794	4-hydroxyphenylacetate degradation	50	5 of 10
66794	coenzyme M biosynthesis	50	5 of 10
66794	ribulose monophosphate pathway	50	1 of 2
66794	toluene degradation	50	2 of 4
66794	lactate fermentation	50	2 of 4
66794	ethanol fermentation	50	1 of 2
66794	butanoate fermentation	50	2 of 4
66794	ketogluconate metabolism	50	4 of 8
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	methionine metabolism	50	13 of 26
66794	cysteine metabolism	50	9 of 18
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	glutathione metabolism	50	7 of 14
66794	quinate degradation	50	1 of 2
66794	sulfate reduction	46.15	6 of 13
66794	proline metabolism	45.45	5 of 11
66794	metabolism of disaccharids	45.45	5 of 11
66794	non-pathway related	44.74	17 of 38
66794	CO2 fixation in Crenarchaeota	44.44	4 of 9
66794	degradation of hexoses	44.44	8 of 18
66794	nitrate assimilation	44.44	4 of 9
66794	degradation of sugar acids	44	11 of 25
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	tyrosine metabolism	42.86	6 of 14
66794	isoprenoid biosynthesis	42.31	11 of 26
66794	methanogenesis from CO2	41.67	5 of 12
66794	polyamine pathway	39.13	9 of 23
66794	phosphatidylethanolamine bioynthesis	38.46	5 of 13
66794	pentose phosphate pathway	36.36	4 of 11
66794	citric acid cycle	35.71	5 of 14
66794	cyanate degradation	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	allantoin degradation	33.33	3 of 9
66794	IAA biosynthesis	33.33	1 of 3
66794	enterobactin biosynthesis	33.33	1 of 3
66794	vitamin B12 metabolism	32.35	11 of 34
66794	lipid metabolism	32.26	10 of 31
66794	phenol degradation	30	6 of 20
66794	degradation of pentoses	28.57	8 of 28
66794	ascorbate metabolism	27.27	6 of 22
66794	d-xylose degradation	27.27	3 of 11
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	ppGpp biosynthesis	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	4-hydroxymandelate degradation	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Industrial wastewater
#Condition	#Anoxic (anaerobic)	-
#Engineered	#Waste	#Wastewater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
4960	anaerobic wool-scouring wastewater treatment lagoon	Trinidad	Uruguay	URY	Middle and South America
43422	wool-scouring wastewater treatment lagoon	Uruguay	Uruguay	URY	Middle and South America	-33.506	-56.889 -33.506/-56.889
43698	The sludge of an anaerobic lagoon treating wool-scouring wastewater	Uruguay	Uruguay	URY	Middle and South America
67770	Sludge of an anaerobic lagoon treating wool-scouring wastewater

Taxonmaps

69479

Global distribution of 16S sequence AB910748 (>99% sequence identity) for Acetomicrobium from Microbeatlas

Aquatic Samples	1383
Soil Samples	172
Animal Samples	498
Plant Samples	81
Total Samples	2134

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4960	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM26692v1 assembly for Acetomicrobium mobile DSM 13181	complete	GCA_000266925	891968.3	2509601011	891968	72.94

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Anaerobaculum mobile gene for 16S ribosomal RNA, partial sequence	AB910748	1433		97477
4960	Anaerobaculum mobile 16S rRNA gene, type strain NGA	AJ243189	1596		891968

GC content

@ref	GC-content (mol%)	Method
4960	51.5	high performance liquid chromatography (HPLC)
43422	51.5

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Acetomicrobium mobile DSM 13181
NCBI: GCA_000266925

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	74.70	no
125439	motility	BacteriaNetⓘ	yes	70.90	no
125439	gram_stain	BacteriaNetⓘ	variable	65.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Acetomicrobium mobile DSM 13181 DSM 13181
NCBI: GCA_000266925.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	85.59	yes
125438	anaerobic	anaerobicⓘ	yes	92.10	yes
125438	aerobic	aerobicⓘ	no	96.32	yes
125438	spore-forming	spore-formingⓘ	no	78.47	yes
125438	thermophilic	thermophileⓘ	yes	74.48	yes
125438	flagellated	motile2+ⓘ	yes	65.07	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Diverse non-canonical electron bifurcating [FeFe]-hydrogenases of separate evolutionary origins in Hydrogenedentota.	Zheng X, Huang L.	mSystems	10.1128/msystems.00999-24	2024
	Identification of a Putative CodY Regulon in the Gram-Negative Phylum Synergistetes.	Geng J, Luo S, Shieh HR, Wang HY, Hu S, Chen YM.	Int J Mol Sci	10.3390/ijms23147911	2022
	Structure and electron transfer pathways of an electron-bifurcating NiFe-hydrogenase.	Feng X, Schut GJ, Haja DK, Adams MWW, Li H.	Sci Adv	10.1126/sciadv.abm7546	2022
Metabolism	The Sporomusa type Nfn is a novel type of electron-bifurcating transhydrogenase that links the redox pools in acetogenic bacteria.	Kremp F, Roth J, Muller V.	Sci Rep	10.1038/s41598-020-71038-2	2020
Metabolism	Tungsten enzymes play a role in detoxifying food and antimicrobial aldehydes in the human gut microbiome.	Schut GJ, Thorgersen MP, Poole FL, Haja DK, Putumbaka S, Adams MWW.	Proc Natl Acad Sci U S A	10.1073/pnas.2109008118	2021
	Evolution and Functional Diversification of Serine Racemase Homologs in Bacteria.	Uda K, Nishimura R, Li Y, Shimoda E, Miyamoto T, Moe LA.	J Mol Evol	10.1007/s00239-024-10231-7	2025
Phylogeny	Reclassification of Anaerobaculum mobile, Anaerobaculum thermoterrenum, Anaerobaculum hydrogeniformans as Acetomicrobium mobile comb. nov., Acetomicrobium thermoterrenum comb. nov. and Acetomicrobium hydrogeniformans comb. nov., respectively, and emendation of the genus Acetomicrobium.	Hania WB, Bouanane-Darenfed A, Cayol JL, Ollivier B, Fardeau ML	Int J Syst Evol Microbiol	10.1099/ijsem.0.000910	2016
Phylogeny	Anaerobaculum mobile sp. nov., a novel anaerobic, moderately thermophilic, peptide-fermenting bacterium that uses crotonate as an electron acceptor, and emended description of the genus Anaerobaculum.	Menes RJ, Muxi L	Int J Syst Evol Microbiol	10.1099/00207713-52-1-157	2002

References

#4960	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13181
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#43422	Konstantinos Mavromatis, Erko Stackebrandt, Brittany Held, Alla Lapidus, Matt Nolan, Susan Lucas, Nancy Hammon, Shweta Deshpande, Jan-Fang Cheng, Roxanne Tapia, Lynne A. Goodwin, Sam Pitluck, Konstantinos Liolios, Ioanna Pagani, Natalia Ivanova, Natalia Mikhailova, Marcel Huntemann, Amrita Pati, Amy Chen, Krishna Palaniappan, Miriam Land, Manfred Rohde, Stefan Spring, Markus Göker, Tanja Woyke, John C. Detter, James Bristow, Jonathan A. Eisen, Victor Markowitz, Philip Hugenholtz, Hans- Peter Klenk, and Nikos C. Kyrpides: Complete genome sequence of the moderate thermophile Anaerobaculum mobile type strain (NGA). Stand Genomic Sci 8: 47 - 57 2013 ( DOI 10.4056/sigs.3547050 , PubMed 23961311 )
#43698	Wajdi Ben Hania, Amel Bouanane-Darenfed, Jean-Luc Cayol, Bernard Ollivier, Marie-Laure Fardeau: Reclassification of Anaerobaculum mobile, Anaerobaculum thermoterrenum, Anaerobaculum hydrogeniformans as Acetomicrobium mobile comb. nov., Acetomicrobium thermoterrenum comb. nov. and Acetomicrobium hydrogeniformans comb. nov., respectively, and emendation of the genus Acetomicrobium. IJSEM 66: 1506 - 1509 2016 ( DOI 10.1099/ijsem.0.000910 , PubMed 26791251 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Acetomicrobium mobile (DSM 13181, ATCC BAA-54, JCM 12221)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Metabolite utilization

Metabolite production

Enzymes

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000266925

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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