Georgenia thermotolerans (DSM 21501, NBRC 104148, TT 02-04)

Name: Georgenia thermotolerans (DSM 21501, NBRC 104148, TT 02-04)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1774

Type strain

Strain Designation TT 02-04

Culture col. no. DSM 21501 NBRC 104148 TT 02-04

NCBI tax ID(s) 527326

Special Collections DSMZ Wink compendium Literature strains

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Georgenia thermotolerans TT 02-04 is a microaerophile, mesophilic, Gram-positive prokaryote that was isolated from forest soil.

Gram-positive motile rod-shaped microaerophile mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 400883

NBRC 104148,DSM 21501

@ref 20215

Last LPSN update 2025-12-16 09:47:11

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Allobogoriellaceae

Genus Georgenia

Species Georgenia thermotolerans

Full scientific name Georgenia thermotolerans Hamada et al. 2009

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape
29057	positive	1.5 µm	0.6 µm	rod-shaped
125439	positive
125438	positive

Colony morphology

@ref	Colony color	Incubation period	Medium used
18343	Broom yellow (1032)	10-14 days	ISP 2
18343	Sulfur yellow (1016)	10-14 days	ISP 3
18343	Beige (1001)	10-14 days	ISP 4
18343	Ivory (1014)	10-14 days	ISP 5
18343	Broom yellow (1032)	10-14 days	ISP 6
18343	Oyster white (1013)	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18343		ISP 2
18343		ISP 3
18343		ISP 4
18343		ISP 5
18343		ISP 6
18343		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
15680	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18343	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18343	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18343	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18343	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18343	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18343	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
15680	positive	growth	28	mesophilic
18343	positive	optimum	28	mesophilic
29057	positive	growth	25-45
29057	positive	optimum	28	mesophilic

Culture pH

@ref	Ability	Type	PH
29057	positive	optimum	7
29057	positive	growth	06-09

Physiology and metabolism

Oxygen tolerance

29057

Oxygen tolerancemicroaerophile

Spore formation

29057

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29057	NaCl	positive	growth	<10 %
29057	NaCl	positive	optimum	0-5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity
18343	22599 ChEBI	arabinose	-
29057	17057 ChEBI	cellobiose	+
18343	62968 ChEBI	cellulose	-
68379	16899 ChEBI	D-mannitol	-	from API Coryne
68379	4853 ChEBI	esculin	+	from API Coryne
18343	28757 ChEBI	fructose	+
68379	5291 ChEBI	gelatin	-	from API Coryne
18343	17234 ChEBI	glucose	-
29057	17234 ChEBI	glucose	+
68379	28087 ChEBI	glycogen	-	from API Coryne
68379	17716 ChEBI	lactose	-	from API Coryne
29057	17306 ChEBI	maltose	+
18343	29864 ChEBI	mannitol	-
18343	17268 ChEBI	myo-inositol	-
29057	17632 ChEBI	nitrate	+
18343	16634 ChEBI	raffinose	-
18343	26546 ChEBI	rhamnose	-
29057	33942 ChEBI	ribose	+
29057	17814 ChEBI	salicin	+
18343	17992 ChEBI	sucrose	-
29057	17992 ChEBI	sucrose	+
68379	17992 ChEBI	sucrose	+	from API Coryne
68379	16199 ChEBI	urea	-	from API Coryne
18343	18222 ChEBI	xylose	-
29057	18222 ChEBI	xylose	+

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	+	3.2.1.31	from API zym
68379	beta-glucuronidase	+	3.2.1.31	from API Coryne
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	-		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
29057	pyrazinamidase	+	3.5.1.B15
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
68379	urease	-	3.5.1.5	from API Coryne
68382	valine arylamidase	+		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
18343	+/-	+	+	+	+	-	+	-	+	-	-	not determinedn.d.	+/-	+/-	+/-	-	+/-	-	+	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18343	not determinedn.d.	+	+	+	-	+	+	-	-	-	+	+	-	-	+	+	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
15680	forest soil	Iriomote Island, Okinawa	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AB436534 (>99% sequence identity) for Georgenia from Microbeatlas

Aquatic Samples	36
Soil Samples	599
Animal Samples	20
Plant Samples	50
Total Samples	705

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15680	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM919318v1 assembly for Georgenia thermotolerans NBRC 104148	contig	GCA_009193185	527326.3	2995314968	527326	52.65
66792	ASM929930v1 assembly for Georgenia thermotolerans DSM 21501	contig	GCA_009299305	527326.4	8065974773	527326	10.76

Genome browser: GCA_009193185

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15680	Georgenia thermotolerans gene for 16S rRNA, partial sequence	AB436534	1480		527326

GC content

@ref	GC-content (mol%)
15680	73.0
29057	73

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Georgenia thermotolerans NBRC 104148
NCBI: GCA_009193185

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	55.10	no
125439	motility	BacteriaNetⓘ	no	81.00	no
125439	gram_stain	BacteriaNetⓘ	positive	99.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	88.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Georgenia thermotolerans strain DSM 21501
PATRIC: 527326.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	91.66	yes
125438	anaerobic	anaerobicⓘ	no	92.07	no
125438	spore-forming	spore-formingⓘ	no	69.07	yes
125438	aerobic	aerobicⓘ	yes	65.60	yes
125438	thermophilic	thermophileⓘ	no	93.74	no
125438	flagellated	motile2+ⓘ	no	67.08	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Georgenia thermotolerans sp. nov., an actinobacterium isolated from forest soil.	Hamada M, Tamura T, Ishida Y, Suzuki K	Int J Syst Evol Microbiol	10.1099/ijs.0.005140-0	2009

References

#15680	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21501
#18343	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#29057	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25487 (see below)
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Georgenia thermotolerans (DSM 21501, NBRC 104148, TT 02-04)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_009193185

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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