Name: Nitriliruptor alkaliphilus ANL-iso2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17693

Type strain:

Species: Nitriliruptor alkaliphilus

Strain Designation: ANL-iso2

Culture col. no.: DSM 45188, NBRC 105792, NCCB 100119, UNIQEM U239

Strain history: <- D. Y. Sorokin; ANL-iso2

NCBI tax ID(s): 1069448 (strain), 427918 (species)

SI-ID 403647

DSM 45188

Section

Nitriliruptor alkaliphilus ANL-iso2 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soda lake sediments.

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Nitriliruptoria*
	Order	*Nitriliruptorales*
	Family	*Nitriliruptoraceae*
	Genus	*Nitriliruptor*
	Species	**Nitriliruptor alkaliphilus**
	Full Scientific Name (LPSN)	Nitriliruptor alkaliphilus Sorokin et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #28940]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %99.309

[Ref.: #28940]	Cell length	2.25 µm

[Ref.: #28940]	Cell width	0.4 µm

[Ref.: #28940]	Cell shape	rod-shaped

[Ref.: #28940]	Motility	no

[Ref.: #28940]

Pigment production

Multimedia content

[Ref.: #15647]

Caption:

Medium 1105 28°C

[Ref.: #15647]

Intellectual property rights:

[Ref.: #66793]

Caption:

electron microscopic image

[Ref.: #66793]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15647]

Culture medium

NITRILIRUPTOR ALKALIPHILUS MEDIUM (DSMZ Medium 1105)

[Ref.: #15647]

Culture medium growth

[Ref.: #15647]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15647]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #15647]	growth	28 °C
[Ref.: #28940]	growth	32 °C
[Ref.: #28940]	optimum	32 °C

[Ref.: #15647]	Temperature range	mesophilic
[Ref.: #28940]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #28940]			optimum	9.25
[Ref.: #28940]			growth	8.2-10.6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #28940]

Oxygen tolerance

aerobe

[Ref.: #28940]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %90
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.965

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #28940]		NaCl		growth	0.38-11.6	%
[Ref.: #28940]		NaCl		optimum	1.45	%

[Ref.: #28940]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #28940]	acetate ChEBI	+	carbon source
[Ref.: #28940]	arabinose ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #28940]	cellobiose ChEBI	+	carbon source
[Ref.: #28940]	citrate ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #28940]	fructose ChEBI	+	carbon source
[Ref.: #28940]	gelatin ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #28940]	glucose ChEBI	+	carbon source
[Ref.: #28940]	glycerol ChEBI	+	carbon source
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #28940]	malate ChEBI	+	carbon source
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #28940]	maltose ChEBI	+	carbon source
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #28940]	mannose ChEBI	+	carbon source
[Ref.: #28940]	myo-inositol ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #28940]	propionate ChEBI	+	carbon source
[Ref.: #28940]	pyruvate ChEBI	+	carbon source
[Ref.: #28940]	succinate ChEBI	+	carbon source
[Ref.: #28940]	sucrose ChEBI	+	carbon source
[Ref.: #28940]	trehalose ChEBI	+	carbon source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #28940]	valerate ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #28940]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #28940]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #15647]	25395	-	-	-	-	-	+/-	-	+	-	-	-	-	-	-	-	-	+/-	-	+/-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #15647]	14359	-	-	+/-	+/-	-	+	+/-	-	+/-	-	-	-	-	-	-	-	+/-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15647]	Sample type/isolated from	soda lake sediments
[Ref.: #15647]	Geographic location (country and/or sea, region)	s-w Siberia
[Ref.: #15647]	Country	Russia
[Ref.: #15647]	Country ISO 3 Code	RUS
[Ref.: #15647]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment
#Condition	#Alkaline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF422408 (>99% sequence identity) for Nitriliruptor alkaliphilus subclade from Microbeatlas

Aquatic Samples	391
Soil Samples	705
Animal Samples	536
Plant Samples	64
Total Samples	1696

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15647]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15647]	Nitriliruptor alkaliphilus strain ANL-iso2 16S ribosomal RNA gene, partial sequence	EF422408	1425	ENA	1069448 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Nitriliruptor alkaliphilus DSM 45188	GCA_000969705	scaffold	GenBank	1069448 tax ID	*
[Ref.: #66792]	Nitriliruptor alkaliphilus DSM 45188	1069448.9	wgs	PATRIC	1069448 tax ID	*
[Ref.: #66792]	Nitriliruptor alkaliphilus DSM 45188	2513020036	draft	JGI IMG/M	1069448 tax ID	*

[Ref.: #15647]	GC-content	70.8 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #28940]	GC-content	70.8 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nitriliruptor alkaliphilus DSM 45188 DSM 45188 NCBI: GCA_000969705.1
[Ref.: #69481]	spore-forming	no	90	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Nitriliruptor alkaliphilus DSM 45188 DSM 45188 NCBI: GCA_000969705.1
[Ref.: #69480]	motile	no	62.43	yes
[Ref.: #69480]	flagellated	no	88.333	yes
[Ref.: #69480]	gram-positive	yes	77.57	no
[Ref.: #69480]	anaerobic	no	96.565	no
[Ref.: #69480]	aerobic	yes	85.669	yes
[Ref.: #69480]	halophile	no	89.485	yes
[Ref.: #69480]	spore-forming	no	84.683	yes
[Ref.: #69480]	thermophile	no	93.508	yes
[Ref.: #69480]	glucose-util	yes	86.751	no
[Ref.: #69480]	glucose-ferment	no	87.838	no

Availability in culture collections External links

[Ref.: #15647]

Culture collection no.

DSM 45188, NBRC 105792, NCCB 100119, UNIQEM U239

[Ref.: #86684]

SI-ID 403647

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nitriliruptor alkaliphilus gen. nov., sp. nov., a deep-lineage haloalkaliphilic actinobacterium from soda lakes capable of growth on aliphatic nitriles, and proposal of Nitriliruptoraceae fam. nov. and Nitriliruptorales ord. nov.	Sorokin DY, van Pelt S, Tourova TP, Evtushenko LI	Int J Syst Evol Microbiol	10.1099/ijs.0.002204-0	2009	*
Genetics	Comparative genomics analysis of Nitriliruptoria reveals the genomic differences and salt adaptation strategies.	Chen DD, Tian Y, Jiao JY, Zhang XT, Zhang YG, Dong ZY, Xiong MJ, Xiao M, Shu WS, Li WJ	Extremophiles	10.1007/s00792-019-01150-3	2019	*
Phylogeny	Egicoccus halophilus gen. nov., sp. nov., a halophilic, alkalitolerant actinobacterium and proposal of Egicoccaceae fam. nov. and Egicoccales ord. nov.	Zhang YG, Chen JY, Wang HF, Xiao M, Yang LL, Guo JW, Zhou EM, Zhang YM, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.000749	2015	*
Phylogeny	Egibacter rhizosphaerae gen. nov., sp. nov., an obligately halophilic, facultatively alkaliphilic actinobacterium and proposal of Egibaceraceae fam. nov. and Egibacterales ord. nov.	Zhang YG, Wang HF, Yang LL, Zhou XK, Zhi XY, Duan YQ, Xiao M, Zhang YM, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.000713	2015	*
Phylogeny	Euzebya tangerina gen. nov., sp. nov., a deeply branching marine actinobacterium isolated from the sea cucumber Holothuria edulis, and proposal of Euzebyaceae fam. nov., Euzebyales ord. nov. and Nitriliruptoridae subclassis nov.	Kurahashi M, Fukunaga Y, Sakiyama Y, Harayama S, Yokota A	Int J Syst Evol Microbiol	10.1099/ijs.0.016543-0	2009	*

References

#15647

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45188

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#28940

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25379 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86684

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403647.1 )

#25379

IJSEM 248 2009 ( DOI 10.1099/ijs.0.002204-0 , PubMed 19196761 )

* These data were automatically processed and therefore are not curated

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Nitriliruptor alkaliphilus DSM 45188 DSM 45188

Nitriliruptor alkaliphilus DSM 45188 DSM 45188

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