Name: Natranaerobius trueperi JW/NM-WN-LU
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17655

Type strain:

Species: Natranaerobius trueperi

Strain Designation: JW/NM-WN-LU

Culture col. no.: DSM 18760, ATCC BAA-1443

Strain history: <- J. Wiegel, Univ. of Georgia, Athens, USA; JW/NM-WN-LU <- N. M. Mesbah {2005}

NCBI tax ID(s): 759412 (species)

SI-ID 405095

DSM 18760

Section

Natranaerobius trueperi JW/NM-WN-LU is an anaerobe, thermophilic, Gram-positive bacterium that was isolated from sediment.

Gram-positive
rod-shaped
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Natranaerobiales*
	Family	*Natranaerobiaceae*
	Genus	*Natranaerobius*
	Species	**Natranaerobius trueperi**
	Full Scientific Name (LPSN)	Natranaerobius trueperi Mesbah and Wiegel 2009

Information on morphological and physiological properties Morphology

[Ref.: #29183]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #29183]	Cell length	2.5 µm

[Ref.: #29183]	Cell width	0.6 µm

[Ref.: #29183]	Cell shape	rod-shaped

[Ref.: #29183]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7607]

Culture medium

NATRANAEROBIUS MEDIUM (DSMZ Medium 1095)

[Ref.: #7607]

Culture medium growth

[Ref.: #7607]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7607]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7607]	growth	50 °C
[Ref.: #29183]	growth	26-55 °C
[Ref.: #29183]	optimum	52 °C

[Ref.: #7607]	Temperature range	thermophilic
[Ref.: #29183]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #29183]			optimum	9.5
[Ref.: #29183]			growth	8.0-10.8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #7607]	Oxygen tolerance	anaerobe
[Ref.: #29183]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.587

[Ref.: #29183]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	yes Confidence in %95.827

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29183]		NaCl		growth	1.5-3.8	%
[Ref.: #29183]		NaCl		optimum	2.2	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29183]	acetate ChEBI	+	carbon source
[Ref.: #29183]	arginine ChEBI	+	carbon source
[Ref.: #29183]	cellobiose ChEBI	+	carbon source
[Ref.: #29183]	D-tryptophan ChEBI	+	carbon source
[Ref.: #29183]	glucose ChEBI	+	carbon source
[Ref.: #29183]	lactate ChEBI	+	carbon source
[Ref.: #29183]	nitrate ChEBI	+	reduction
[Ref.: #29183]	pyruvate ChEBI	+	carbon source
[Ref.: #29183]	ribose ChEBI	+	carbon source
[Ref.: #29183]	sucrose ChEBI	+	carbon source

	Metabolite production	Metabolite	Production
[Ref.: #29183]		hydrogen sulfide ChEBI	yes

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	reductive acetyl coenzyme A pathway	100	7 of 7
[Ref.: #66794]	C4 and CAM-carbon fixation	100	8 of 8
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	aminopropanol phosphate biosynthesis	100	2 of 2
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	gluconeogenesis	87.5	7 of 8
[Ref.: #66794]	palmitate biosynthesis	86.36	19 of 22
[Ref.: #66794]	vitamin B1 metabolism	84.62	11 of 13
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	lipoate biosynthesis	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	threonine metabolism	80	8 of 10
[Ref.: #66794]	glycine betaine biosynthesis	80	4 of 5
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	pyrimidine metabolism	77.78	35 of 45
[Ref.: #66794]	serine metabolism	77.78	7 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	77.78	7 of 9
[Ref.: #66794]	urea cycle	76.92	10 of 13
[Ref.: #66794]	purine metabolism	75.53	71 of 94
[Ref.: #66794]	biotin biosynthesis	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	vitamin B12 metabolism	73.53	25 of 34
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	NAD metabolism	72.22	13 of 18
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	propanol degradation	71.43	5 of 7
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	histidine metabolism	68.97	20 of 29
[Ref.: #66794]	glutamate and glutamine metabolism	67.86	19 of 28
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	enterobactin biosynthesis	66.67	2 of 3
[Ref.: #66794]	glycolate and glyoxylate degradation	66.67	4 of 6
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	d-mannose degradation	66.67	6 of 9
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	molybdenum cofactor biosynthesis	66.67	6 of 9
[Ref.: #66794]	heme metabolism	64.29	9 of 14
[Ref.: #66794]	tetrahydrofolate metabolism	64.29	9 of 14
[Ref.: #66794]	glutathione metabolism	64.29	9 of 14
[Ref.: #66794]	alanine metabolism	62.07	18 of 29
[Ref.: #66794]	phenylalanine metabolism	61.54	8 of 13
[Ref.: #66794]	polyamine pathway	60.87	14 of 23
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	factor 420 biosynthesis	60	3 of 5
[Ref.: #66794]	hydrogen production	60	3 of 5
[Ref.: #66794]	propionate fermentation	60	6 of 10
[Ref.: #66794]	isoprenoid biosynthesis	57.69	15 of 26
[Ref.: #66794]	ubiquinone biosynthesis	57.14	4 of 7
[Ref.: #66794]	non-pathway related	55.26	21 of 38
[Ref.: #66794]	proline metabolism	54.55	6 of 11
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	53.85	7 of 13
[Ref.: #66794]	cysteine metabolism	50	9 of 18
[Ref.: #66794]	sulfopterin metabolism	50	2 of 4
[Ref.: #66794]	suberin monomers biosynthesis	50	1 of 2
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	adipate degradation	50	1 of 2
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	50	6 of 12
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	methionine metabolism	50	13 of 26
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	arginine metabolism	45.83	11 of 24
[Ref.: #66794]	lipid metabolism	45.16	14 of 31
[Ref.: #66794]	oxidative phosphorylation	45.05	41 of 91
[Ref.: #66794]	CO2 fixation in Crenarchaeota	44.44	4 of 9
[Ref.: #66794]	lipid A biosynthesis	44.44	4 of 9
[Ref.: #66794]	citric acid cycle	42.86	6 of 14
[Ref.: #66794]	lysine metabolism	42.86	18 of 42
[Ref.: #66794]	tryptophan metabolism	42.11	16 of 38
[Ref.: #66794]	methylglyoxal degradation	40	2 of 5
[Ref.: #66794]	glycogen metabolism	40	2 of 5
[Ref.: #66794]	Entner Doudoroff pathway	40	4 of 10
[Ref.: #66794]	starch degradation	40	4 of 10
[Ref.: #66794]	degradation of pentoses	39.29	11 of 28
[Ref.: #66794]	degradation of hexoses	38.89	7 of 18
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	37.5	3 of 8
[Ref.: #66794]	ketogluconate metabolism	37.5	3 of 8
[Ref.: #66794]	isoleucine metabolism	37.5	3 of 8
[Ref.: #66794]	pentose phosphate pathway	36.36	4 of 11
[Ref.: #66794]	vitamin B6 metabolism	36.36	4 of 11
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	allantoin degradation	33.33	3 of 9
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	leucine metabolism	30.77	4 of 13
[Ref.: #66794]	sulfate reduction	30.77	4 of 13
[Ref.: #66794]	phenylpropanoid biosynthesis	30.77	4 of 13
[Ref.: #66794]	tyrosine metabolism	28.57	4 of 14
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	d-xylose degradation	27.27	3 of 11
[Ref.: #66794]	alginate biosynthesis	25	1 of 4
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	degradation of sugar alcohols	25	4 of 16
[Ref.: #66794]	carnitine metabolism	25	2 of 8
[Ref.: #66794]	valine metabolism	22.22	2 of 9
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7607]	Sample type/isolated from	sediment
[Ref.: #7607]	Geographic location (country and/or sea, region)	Wadi An Natrun, Lake UmRisha
[Ref.: #7607]	Country	Egypt
[Ref.: #7607]	Country ISO 3 Code	EGY
[Ref.: #7607]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Sediment
#Environmental	#Terrestrial	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU338490 (>99% sequence identity) for Natranaerobius trueperi subclade from Microbeatlas

Aquatic Samples	15
Soil Samples	3
Animal Samples
Plant Samples
Total Samples	18

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7607]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7607]	Natranaerobius trueperi strain JW/NM-WN-LU 16S ribosomal RNA gene, partial sequence	EU338490	1424	ENA	759412 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Natranaerobius trueperi DSM 18760	GCA_002216005	contig	GenBank	759412 tax ID	*
[Ref.: #66792]	Natranaerobius trueperi strain DSM 18760	759412.3	wgs	PATRIC	759412 tax ID	*
[Ref.: #66792]	Natranaerobius trueperi DSM 18760	2889473178	draft	JGI IMG/M	759412 tax ID	*

[Ref.: #7607]	GC-content	41 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29183]	GC-content	41 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Natranaerobius trueperi strain DSM 18760 PATRIC: 759412.3
[Ref.: #69481]	spore-forming	yes	85	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Natranaerobius trueperi strain DSM 18760 PATRIC: 759412.3
[Ref.: #69480]	motile	yes	79.253	no
[Ref.: #69480]	flagellated	no	88.196	yes
[Ref.: #69480]	gram-positive	no	61.695	no
[Ref.: #69480]	anaerobic	yes	98.202	yes
[Ref.: #69480]	aerobic	no	93.207	no
[Ref.: #69480]	halophile	no	74.348	no
[Ref.: #69480]	spore-forming	yes	85.968	no
[Ref.: #69480]	glucose-util	yes	83.441	no
[Ref.: #69480]	thermophile	yes	90.832	no
[Ref.: #69480]	glucose-ferment	no	73.979	no

Availability in culture collections External links

[Ref.: #7607]

Culture collection no.

DSM 18760, ATCC BAA-1443

[Ref.: #86646]

SI-ID 405095

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequence of Natranaerobius trueperi DSM 18760(T), an Anaerobic, Halophilic, Alkaliphilic, Thermotolerant Bacterium Isolated from a Soda Lake.	Guo X, Liao Z, Holtzapple M, Hu Q, Zhao B	Genome Announc	10.1128/genomeA.00785-17	2017	*
Phylogeny	Natronovirga wadinatrunensis gen. nov., sp. nov. and Natranaerobius trueperi sp. nov., halophilic, alkalithermophilic micro-organisms from soda lakes of the Wadi An Natrun, Egypt.	Mesbah NM, Wiegel J	Int J Syst Evol Microbiol	10.1099/ijs.0.008151-0	2009	*

References

#7607

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18760

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29183

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25607 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86646

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405095.1 )

#25607

IJSEM 2042 2009 ( DOI 10.1099/ijs.0.008151-0 , PubMed 19605718 )

* These data were automatically processed and therefore are not curated

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Natranaerobius trueperi strain DSM 18760

Natranaerobius trueperi strain DSM 18760

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