Name: Actinospica robiniae GE134769
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17635

Type strain:

Species: Actinospica robiniae

Strain Designation: GE134769

Culture col. no.: DSM 44927, JCM 14908, NRRL B-24432, BCRC 16839, NBRC 102112

Strain history: DSM 44927 <-- L. Cavaletti GE134769.

NCBI tax ID(s): 479430 (strain), 304901 (species)

SI-ID 343938

NRRL B-24432, DSM 44927, BCRC 16839, CCRC 16839, NBRC 102112, JCM 14908

Section

Actinospica robiniae GE134769 is an aerobe, spore-forming, mesophilic bacterium that was isolated from forest soil.

Gram-positive
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Catenulisporales*
	Family	*Actinospicaceae*
	Genus	*Actinospica*
	Species	**Actinospica robiniae**
	Full Scientific Name (LPSN)	Actinospica robiniae Cavaletti et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31589]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #31589]	Cell length	1-1.2 µm

[Ref.: #31589]	Cell width	0.6 µm

[Ref.: #31589]	Cell shape	rod-shaped

[Ref.: #31589]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %95.667

[Ref.: #31589]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #12200]

Culture medium

ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)

[Ref.: #12200]

Culture medium growth

[Ref.: #12200]

Culture medium link

Medium recipe at MediaDive

[Ref.: #12200]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #12200]	growth	28 °C
[Ref.: #31589]	growth	17-33 °C
[Ref.: #31589]	optimum	22-28 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #12200]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31589]			optimum	5.5
[Ref.: #31589]			growth	4.8-6.2

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31589]

Oxygen tolerance

aerobe

[Ref.: #31589]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.729

[Ref.: #31589]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-9(H₄), MK-9(H₆), MK-9(H₈)

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43413]		cellulose ChEBI	+	degradation

	Metabolite production	Metabolite	Production
[Ref.: #31589]		hydrogen sulfide ChEBI	yes

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #31589]		catalase	+	1.11.1.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #12200]	Sample type/isolated from	forest soil
[Ref.: #12200]	Geographic location (country and/or sea, region)	Lombardia, Gerenzano
[Ref.: #12200]	Country	Italy
[Ref.: #12200]	Country ISO 3 Code	ITA
[Ref.: #12200]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Soil in a wooded area
[Ref.: #67770]	Geographic location (country and/or sea, region)	Gerenzano
[Ref.: #67770]	Country	Italy
[Ref.: #67770]	Country ISO 3 Code	ITA
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ865863 (>99% sequence identity) for Actinospica robiniae from Microbeatlas

Aquatic Samples	342
Soil Samples	4561
Animal Samples	820
Plant Samples	697
Total Samples	6420

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12200]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #12200]	Actinospica robiniae partial 16S rRNA gene, type strain GE134769T	AJ865863	1444	ENA	479430 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Actinospica robiniae DSM 44927	GCA_000504285	scaffold	GenBank	479430 tax ID
[Ref.: #66792]	Actinospica robiniae DSM 44927	479430.3	wgs	PATRIC	479430 tax ID	*
[Ref.: #66792]	Actinospica robiniae DSM 44927	2510065062	draft	JGI IMG/M	479430 tax ID	*

[Ref.: #12200]	GC-content	70.8 mol%
[Ref.: #67770]	GC-content	70.8 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinospica robiniae DSM 44927 DSM 44927 NCBI: GCA_000504285.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinospica robiniae DSM 44927 PATRIC: 479430.3
[Ref.: #69480]	gram-positive	yes	88.035	yes
[Ref.: #69480]	anaerobic	no	99.046	yes
[Ref.: #69480]	halophile	no	95.923	no
[Ref.: #69480]	spore-forming	yes	93.881	no
[Ref.: #69480]	glucose-util	yes	86.258	no
[Ref.: #69480]	aerobic	yes	90.907	no
[Ref.: #69480]	thermophile	no	98.34	yes
[Ref.: #69480]	motile	no	94.269	yes
[Ref.: #69480]	flagellated	no	98.114	yes
[Ref.: #69480]	glucose-ferment	no	85.092	no

Availability in culture collections External links

[Ref.: #12200]

Culture collection no.

DSM 44927, JCM 14908, NRRL B-24432, BCRC 16839, NBRC 102112

[Ref.: #86627]

SI-ID 343938

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinospica durhamensis sp. nov., isolated from a spruce forest soil.	Golinska P, Zucchi TD, Silva L, Dahm H, Goodfellow M	Antonie Van Leeuwenhoek	10.1007/s10482-015-0496-1	2015	*
Phylogeny	Actinospica robiniae gen. nov., sp. nov. and Actinospica acidiphila sp. nov.: proposal for Actinospicaceae fam. nov. and Catenulisporinae subord. nov. in the order Actinomycetales.	Cavaletti L, Monciardini P, Schumann P, Rohde M, Bamonte R, Busti E, Sosio M, Donadio S	Int J Syst Evol Microbiol	10.1099/ijs.0.63859-0	2006	*

References

#12200

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44927

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31589

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27878 (see below)

#43413

Iain Anderson, Birte Abt, Athanasios Lykidis, Hans-Peter Klenk, Nikos Kyrpides, Natalia Ivanova: Genomics of Aerobic Cellulose Utilization Systems in Actinobacteria. PLoS ONE 7: 2012 ( DOI 10.1371/journal.pone.0039331 , PubMed 22723998 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86627

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID343938.1 )

#27878

IJSEM 1747 2006 ( DOI 10.1099/ijs.0.63859-0 , PubMed 16902002 )

* These data were automatically processed and therefore are not curated

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