Actinospica acidiphila GE134766 | Type strain | DSM 44926, NRRL B-24431, JCM 14896, BCRC 16838, NBRC 102107 | BacDiveID:17634

Strain identifier

BacDive ID: 17634

Type strain:

Species: Actinospica acidiphila

Strain Designation: GE134766

Culture col. no.: DSM 44926, NRRL B-24431, JCM 14896, BCRC 16838, NBRC 102107

Strain history: NBRC 102107 <-- NRRL B-24431 <-- L. Cavaletti GE134766.

NCBI tax ID(s): 304899 (species)

SI-ID 295938

NRRL B-24431, DSM 44926, BCRC 16838, CCRC 16838, NBRC 102107, JCM 14896

Section

Actinospica acidiphila GE134766 is an aerobe, spore-forming, mesophilic bacterium that was isolated from forest soil.

Gram-positive
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Catenulisporales*
	Family	*Actinospicaceae*
	Genus	*Actinospica*
	Species	**Actinospica acidiphila**
	Full Scientific Name (LPSN)	Actinospica acidiphila Cavaletti et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31589]	Gram stain	positive

[Ref.: #31589]	Cell length	0.6-0.8 µm

[Ref.: #31589]	Cell width	0.5 µm

[Ref.: #31589]	Cell shape	rod-shaped

[Ref.: #31589]	Motility	no

[Ref.: #19889]	Colony color	Beige
[Ref.: #19889]	Incubation period	10-14 days
[Ref.: #19889]	Cultivation medium used	ISP 2

[Ref.: #19889]	Colony color	Beige
[Ref.: #19889]	Incubation period	10-14 days
[Ref.: #19889]	Cultivation medium used	ISP 3

[Ref.: #19889]	Incubation period	10-14 days
[Ref.: #19889]	Cultivation medium used	ISP 4

[Ref.: #19889]	Incubation period	10-14 days
[Ref.: #19889]	Cultivation medium used	ISP 5

[Ref.: #19889]	Incubation period	10-14 days
[Ref.: #19889]	Cultivation medium used	ISP 6

[Ref.: #19889]	Incubation period	10-14 days
[Ref.: #19889]	Cultivation medium used	ISP 7

Pigment production

yes

[Ref.: #19889]	Multicellular complex forming ability	no
[Ref.: #19889]	Medium Name (multicellularity)	ISP 2

[Ref.: #19889]	Multicellular complex forming ability	no
[Ref.: #19889]	Medium Name (multicellularity)	ISP 3

[Ref.: #19889]	Multicellular complex forming ability	no
[Ref.: #19889]	Medium Name (multicellularity)	ISP 4

[Ref.: #19889]	Multicellular complex forming ability	no
[Ref.: #19889]	Medium Name (multicellularity)	ISP 5

[Ref.: #19889]	Multicellular complex forming ability	no
[Ref.: #19889]	Medium Name (multicellularity)	ISP 6

[Ref.: #19889]	Multicellular complex forming ability	no
[Ref.: #19889]	Medium Name (multicellularity)	ISP 7

Multimedia content

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Information on culture and growth conditions Culture and growth conditions

Culture medium

ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

Culture medium

ISP2 MEDIUM (DSMZ Medium 987)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

Culture medium

ISP 2

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 3

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 4

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 5

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 6

Culture medium growth

Culture medium composition

expand / minimize

Culture medium

ISP 7

Culture medium growth

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #12199]			growth	28 °C
[Ref.: #31589]			growth	17-33 °C
[Ref.: #31589]			optimum	28 °C
[Ref.: #67770]			growth	28 °C

[Ref.: #12199]	Temperature range	mesophilic
[Ref.: #31589]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31589]			optimum	5
[Ref.: #31589]			growth	4.2-6

Information on physiology and metabolism Physiology and metabolism

Oxygen tolerance

aerobe

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31589]		NaCl		growth	0-1	%
[Ref.: #31589]		NaCl		optimum	0-1	%

[Ref.: #31589]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-9(H₄), MK-9(H₆), MK-9(H₈)

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31589]		arabinose ChEBI	+	carbon source
[Ref.: #31589]		xylose ChEBI	+	carbon source

	Metabolite production	Metabolite	Production
[Ref.: #31589]		hydrogen sulfide ChEBI	yes

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #68382]		acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]		alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]		alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]		alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]		alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]		alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]		alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68382]		beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]		beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]		beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #31589]		catalase	+	1.11.1.6
[Ref.: #68382]		cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]		esterase (C 4)	+		* from API zym
[Ref.: #68382]		esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]		leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]		lipase (C 14)	+		* from API zym
[Ref.: #68382]		N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]		naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68382]		trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]		valine arylamidase	+		* from API zym
		Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19889]	701	not determinedn.d.	+	+	+	+	+	+	-	-	-	+	+	+	+	+	+	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #12199]	Sample type/isolated from	forest soil
[Ref.: #12199]	Geographic location (country and/or sea, region)	Lombardia, Gerenzano
[Ref.: #12199]	Country	Italy
[Ref.: #12199]	Country ISO 3 Code	ITA
[Ref.: #12199]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Soil in a wooded area
[Ref.: #67770]	Geographic location (country and/or sea, region)	Gerenzano
[Ref.: #67770]	Country	Italy
[Ref.: #67770]	Country ISO 3 Code	ITA
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Global distribution of 16S sequence AJ865861 (>99% sequence identity) for Actinospica from Microbeatlas

Aquatic Samples	70
Soil Samples	1356
Animal Samples	76
Plant Samples	134
Total Samples	1636

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12199]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19889]	Biosafety level	1	Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #12199]	Actinospica acidiphila partial 16S rRNA gene, type strain GE134766T	AJ865861	1446	ENA	304899 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Actinospica acidiphila NRRL B-24431	GCA_000717315	contig	GenBank	304899 tax ID

[Ref.: #12199]	GC-content	69.2 mol%
[Ref.: #67770]	GC-content	69.2 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinospica acidiphila NRRL B-24431 NCBI: GCA_000717315.1
[Ref.: #69480]	motile	no	94.363	no
[Ref.: #69480]	flagellated	no	97.681	no
[Ref.: #69480]	gram-positive	yes	91.023	no
[Ref.: #69480]	anaerobic	no	99.305	yes
[Ref.: #69480]	halophile	no	89.193	no
[Ref.: #69480]	spore-forming	yes	95.948	yes
[Ref.: #69480]	thermophile	no	98.181	yes
[Ref.: #69480]	glucose-util	yes	89.07	no
[Ref.: #69480]	aerobic	yes	94.922	yes
[Ref.: #69480]	glucose-ferment	no	87.943	no

Availability in culture collections External links

Culture collection no.

DSM 44926, NRRL B-24431, JCM 14896, BCRC 16838, NBRC 102107

SI-ID 295938

*

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinospica robiniae gen. nov., sp. nov. and Actinospica acidiphila sp. nov.: proposal for Actinospicaceae fam. nov. and Catenulisporinae subord. nov. in the order Actinomycetales.	Cavaletti L, Monciardini P, Schumann P, Rohde M, Bamonte R, Busti E, Sosio M, Donadio S	Int J Syst Evol Microbiol	10.1099/ijs.0.63859-0	2006	*

References

#12199

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44926

#19889

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31589

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27878 (see below)

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#86626

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID295938.1 )

#27878

IJSEM 1747 2006 ( DOI 10.1099/ijs.0.63859-0 , PubMed 16902002 )

* These data were automatically processed and therefore are not curated

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