Methanocalculus sp. (DSM 27074, AMF-Bu2)

Name: Methanocalculus sp. (DSM 27074, AMF-Bu2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 175199

Type strain

Strain Designation AMF-Bu2

Culture col. no. DSM 27074 AMF-Bu2

NCBI tax ID(s) 2004547

Links

AMF-Bu2 is a prokaryote that was isolated from sediments from hypersaline soda lake.

16S sequence

Name and taxonomic classification

SI-ID 406686

DSM 27074

@ref 20215

Last LPSN update 2025-12-16 10:11:59

Domain Methanobacteriati

Phylum Methanobacteriota

Class "Methanomicrobia"

Order Methanomicrobiales

Family Methanocalculaceae

Genus Methanocalculus

Full scientific name Methanocalculus Ollivier et al. 1998

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
124994	METHANOCALCULUS ALKALIPHILUS MEDIUM (DSMZ Medium 1596)		Medium recipe at MediaDive	Name: METHANOCALCULUS ALKALIPHILUS MEDIUM (DSMZ Medium 1596) Composition: NaHCO3 19.9402 g/l Na2CO3 14.9551 g/l Na-formate 3.38983 g/l NaCl 2.99103 g/l K2HPO4 0.997009 g/l MgSO4 x 7 H2O 0.498504 g/l Na2S x 9 H2O 0.249252 g/l NH4Cl 0.199402 g/l Na-acetate 0.159521 g/l Yeast extract 0.0199402 g/l EDTA 0.00498504 g/l FeSO4 x 7 H2O 0.00219342 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000199402 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnSO4 x 7 H2O 9.97009e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l MnCl2 x 4 H2O 2.99103e-05 g/l H3BO3 2.99103e-05 g/l CuCl2 x 2 H2O 2.99103e-05 g/l NiCl2 x 6 H2O 2.99103e-05 g/l Na2MoO4 x 2 H2O 2.99103e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
124994	positive	growth	30

Physiology and metabolism

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
124994	sediments from hypersaline soda lake	Siberia, Altai	Russia	RUS	Europe

Interaction and safety

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
124994	Methanocalculus sp. AMF-Bu2 16S ribosomal RNA gene, partial sequence	JQ724113	1420		1190944

Genome-based predictions

Literature

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#124994	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27074
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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