Bifidobacterium actinocoloniiforme (DSM 22766, CCM 7728, LISLUCIII-P2)

Name: Bifidobacterium actinocoloniiforme (DSM 22766, CCM 7728, LISLUCIII-P2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1749

Type strain

Strain Designation LISLUCIII-P2

Culture col. no. DSM 22766 CCM 7728 LISLUCIII-P2 KCTC 15183 JCM 18048

NCBI tax ID(s) 1437605 638619

Special Collections DSMZ JCM KCTC

History DSMZ <- J. Killer, Inst. Animal Physiology and Genetics, Academy Sci. of the Czech Republic, Prague; LISLUCIII-P2 JCM <-- CCM 7728 <-- J. Killer LISLUCIII-P2.

Links

Bifidobacterium actinocoloniiforme LISLUCIII-P2 is an anaerobe bacterium that was isolated from bumblebee digestive tract.

anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 400411

JCM 18048,DSM 22766

@ref 20215

Last LPSN update 2026-05-29 08:25:20

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Bifidobacteriales

Family Bifidobacteriaceae

Genus Bifidobacterium

Species Bifidobacterium actinocoloniiforme

Full scientific name Bifidobacterium actinocoloniiforme Killer et al. 2011

Morphology

Cell morphology

@ref	Motility	Confidence
125438		94.5

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
16505	BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)		Medium recipe at MediaDive	Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
16505	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
16505	anaerobe
125439	anaerobe	95.015

Spore formation

@ref	Spore formation	Confidence
125438		90.484
125439		97.676

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	+	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	+	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	formaldehyde oxidation	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	adipate degradation	100	2 of 2
66794	acetate fermentation	100	4 of 4
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	valine metabolism	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	palmitate biosynthesis	86.36	19 of 22
66794	pentose phosphate pathway	81.82	9 of 11
66794	peptidoglycan biosynthesis	80	12 of 15
66794	photosynthesis	78.57	11 of 14
66794	sulfopterin metabolism	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	Entner Doudoroff pathway	70	7 of 10
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	acetoin degradation	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	octane oxidation	66.67	2 of 3
66794	glycolysis	64.71	11 of 17
66794	metabolism of disaccharids	63.64	7 of 11
66794	d-xylose degradation	63.64	7 of 11
66794	degradation of sugar alcohols	62.5	10 of 16
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	degradation of pentoses	60.71	17 of 28
66794	glycogen metabolism	60	3 of 5
66794	pyrimidine metabolism	60	27 of 45
66794	reductive acetyl coenzyme A pathway	57.14	4 of 7
66794	oxidative phosphorylation	57.14	52 of 91
66794	mevalonate metabolism	57.14	4 of 7
66794	propanol degradation	57.14	4 of 7
66794	citric acid cycle	57.14	8 of 14
66794	NAD metabolism	55.56	10 of 18
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	d-mannose degradation	55.56	5 of 9
66794	degradation of sugar acids	52	13 of 25
66794	ketogluconate metabolism	50	4 of 8
66794	isoprenoid biosynthesis	50	13 of 26
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	glycogen biosynthesis	50	2 of 4
66794	tetrahydrofolate metabolism	50	7 of 14
66794	ethanol fermentation	50	1 of 2
66794	gluconeogenesis	50	4 of 8
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	purine metabolism	50	47 of 94
66794	coenzyme A metabolism	50	2 of 4
66794	butanoate fermentation	50	2 of 4
66794	non-pathway related	47.37	18 of 38
66794	methionine metabolism	46.15	12 of 26
66794	phenylalanine metabolism	46.15	6 of 13
66794	vitamin B1 metabolism	46.15	6 of 13
66794	proline metabolism	45.45	5 of 11
66794	degradation of hexoses	44.44	8 of 18
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	glutamate and glutamine metabolism	42.86	12 of 28
66794	lipid metabolism	41.94	13 of 31
66794	alanine metabolism	41.38	12 of 29
66794	cellulose degradation	40	2 of 5
66794	threonine metabolism	40	4 of 10
66794	glycine metabolism	40	4 of 10
66794	O-antigen biosynthesis	40	2 of 5
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	lipoate biosynthesis	40	2 of 5
66794	starch degradation	40	4 of 10
66794	urea cycle	38.46	5 of 13
66794	tyrosine metabolism	35.71	5 of 14
66794	tryptophan metabolism	34.21	13 of 38
66794	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
66794	arginine metabolism	33.33	8 of 24
66794	pantothenate biosynthesis	33.33	2 of 6
66794	flavin biosynthesis	33.33	5 of 15
66794	chorismate metabolism	33.33	3 of 9
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	ascorbate metabolism	31.82	7 of 22
66794	sulfate reduction	30.77	4 of 13
66794	leucine metabolism	30.77	4 of 13
66794	4-hydroxyphenylacetate degradation	30	3 of 10
66794	myo-inositol biosynthesis	30	3 of 10
66794	propionate fermentation	30	3 of 10
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	lysine metabolism	28.57	12 of 42
66794	cysteine metabolism	27.78	5 of 18
66794	histidine metabolism	27.59	8 of 29
66794	vitamin B6 metabolism	27.27	3 of 11
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	androgen and estrogen metabolism	25	4 of 16
66794	cyclohexanol degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	dTDPLrhamnose biosynthesis	25	2 of 8
66794	4-hydroxymandelate degradation	22.22	2 of 9
66794	lipid A biosynthesis	22.22	2 of 9
66794	molybdenum cofactor biosynthesis	22.22	2 of 9
66794	nitrate assimilation	22.22	2 of 9
66794	glutathione metabolism	21.43	3 of 14
66794	heme metabolism	21.43	3 of 14

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
16505	-	-	-	-	+	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	+	-	+	+	+	+	+	-	-	+	+/-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
16505	bumblebee (Bombus lucorum) digestive tract	Bombus lucorum	Central Bohemia	Czech Republic	CZE	Europe
67770	Digestive tract contents of a bumblebee (Bombus lucorum) from Central Bohemia	Bombus lucorum		Czech Republic	CZE	Europe

Taxonmaps

69479

Global distribution of 16S sequence LC521980 (>99% sequence identity) for Bifidobacterium actinocoloniiforme subclade from Microbeatlas

Aquatic Samples	377
Soil Samples	217
Animal Samples	20713
Plant Samples	75
Total Samples	21382

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16505	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM126339v1 assembly for Bifidobacterium actinocoloniiforme DSM 22766	complete	GCA_001263395	1437605.7	2645727657	1437605	90.59
67770	Bifact assembly for Bifidobacterium actinocoloniiforme DSM 22766	contig	GCA_000741095	1437605.3	2600254971	1437605	69.65
67770	DSM-22766 assembly for Bifidobacterium actinocoloniiforme DSM 22766	contig	GCA_000771585	1437605.6		1437605	53.23

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16505	Bifidobacterium actinocoloniiforme DSM 22766 16S ribosomal RNA gene, partial sequence	FJ858731	1262		1437605
67770	Bifidobacterium actinocoloniiforme JCM 18048 gene for 16S rRNA, partial sequence	LC521980	1464		638619

GC content

@ref	GC-content (mol%)	Method
16505	52.7	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM126339v1 assembly for Bifidobacterium actinocoloniiforme DSM 22766
BV-BRC: 1437605.7

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	95.02	no
125439	gram_stain	BacteriaNetⓘ	positive	70.70	no
125439	motility	BacteriaNetⓘ	no	87.96	no
125439	spore_formation	BacteriaNetⓘ	no	97.68	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Bifidobacterium actinocoloniiforme DSM 22766 DSM 22766
NCBI: GCA_001263395.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	82.43	no
125438	anaerobic	anaerobicⓘ	yes	64.67	no
125438	aerobic	aerobicⓘ	no	94.98	yes
125438	spore-forming	spore-formingⓘ	no	90.48	no
125438	thermophilic	thermophileⓘ	no	93.99	yes
125438	flagellated	motile2+ⓘ	no	94.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Exploring the biodiversity of Bifidobacterium asteroides among honey bee microbiomes.	Lugli GA, Fontana F, Tarracchini C, Mancabelli L, Milani C, Turroni F, Ventura M.	Environ Microbiol	10.1111/1462-2920.16223	2022
Genetics	Genome-Wide Assessment of Stress-Associated Genes in Bifidobacteria.	Schopping M, Vesth T, Jensen K, Franzen CJ, Zeidan AA.	Appl Environ Microbiol	10.1128/aem.02251-21	2022
Genetics	Metagenomic analysis of soybean endosphere microbiome to reveal signatures of microbes for health and disease.	Chouhan U, Gamad U, Choudhari JK.	J Genet Eng Biotechnol	10.1186/s43141-023-00535-4	2023
Genetics	Comparative genomic and phylogenomic analyses of the Bifidobacteriaceae family.	Lugli GA, Milani C, Turroni F, Duranti S, Mancabelli L, Mangifesta M, Ferrario C, Modesto M, Mattarelli P, Jiri K, van Sinderen D, Ventura M.	BMC Genomics	10.1186/s12864-017-3955-4	2017
Metabolism	Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-alpha-1,6-N-Acetylglucosamine-Asparagine.	Becerra JE, Rodriguez-Diaz J, Gozalbo-Rovira R, Palomino-Schatzlein M, Zuniga M, Monedero V, Yebra MJ.	mBio	10.1128/mbio.02804-19	2020
Pathogenicity	Exploring the Ecology of Bifidobacteria and Their Genetic Adaptation to the Mammalian Gut.	Duranti S, Longhi G, Ventura M, van Sinderen D, Turroni F.	Microorganisms	10.3390/microorganisms9010008	2020
Phylogeny	Phylogenetic Analysis of the Bifidobacterium Genus Using Glycolysis Enzyme Sequences.	Brandt K, Barrangou R.	Front Microbiol	10.3389/fmicb.2016.00657	2016
	Occurrence and Diversity of CRISPR-Cas Systems in the Genus Bifidobacterium.	Briner AE, Lugli GA, Milani C, Duranti S, Turroni F, Gueimonde M, Margolles A, van Sinderen D, Ventura M, Barrangou R.	PLoS One	10.1371/journal.pone.0133661	2015
Metabolism	Diversity, ecology and intestinal function of bifidobacteria.	Bottacini F, Ventura M, van Sinderen D, O'Connell Motherway M.	Microb Cell Fact	10.1186/1475-2859-13-s1-s4	2014
Phylogeny	Genomic encyclopedia of type strains of the genus Bifidobacterium.	Milani C, Lugli GA, Duranti S, Turroni F, Bottacini F, Mangifesta M, Sanchez B, Viappiani A, Mancabelli L, Taminiau B, Delcenserie V, Barrangou R, Margolles A, van Sinderen D, Ventura M.	Appl Environ Microbiol	10.1128/aem.02308-14	2014
Genetics	Complete Genome Sequence of Bifidobacterium actinocoloniiforme Type Strain DSM 22766T, Isolated from Bumblebee Digestive Tracts.	Chen X, E Z, Gu D, Lv L, Li Y	Genome Announc	10.1128/genomeA.01084-15	2015
Phylogeny	Bifidobacterium actinocoloniiforme sp. nov. and Bifidobacterium bohemicum sp. nov., from the bumblebee digestive tract.	Killer J, Kopecny J, Mrazek J, Koppova I, Havlik J, Benada O, Kott T	Int J Syst Evol Microbiol	10.1099/ijs.0.022525-0	2010

References

#16505	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22766
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Bifidobacterium actinocoloniiforme (DSM 22766, CCM 7728, LISLUCIII-P2)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Pathway annotation

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001263395

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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