Bifidobacterium animalis subsp. animalis (DSM 20104, ATCC 25527, CIP 105419)

Name: Bifidobacterium animalis subsp. animalis (DSM 20104, ATCC 25527, CIP 105419)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1736

Type strain

Strain Designation R101-8

Culture col. no. DSM 20104 ATCC 25527 CIP 105419 R101-8 VTT E-96663 8 more

NCBI tax ID(s) 703613 28025 302912

Special Collections DSMZ CCUG JCM KCTC CIP

History <- T. Mitsuoka, R101-8 (Bifidobacterium longum subsp. animalis biotype a) ATCC 25527 <-- T. Mitsuoka R 101-8 ("Bifidobacterium longum subsp. animalis" biotype a). CIP <- 1998, DSM <- T. Mitsuoka: strain R101-8, Bifidobacterium longum subsp. animalis biotype a

Links

Bifidobacterium animalis subsp. animalis R101-8 is an anaerobe, mesophilic, Gram-positive prokaryote that was isolated from rat faeces.

Gram-positive anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 1236

LMG 10508,ATCC 25527,CCRC 14668,CCUG 24606,CCUG 33907,DSM 20104,JCM 1190,KCTC 3125,LMG 11083,LMG 17135,NCFB 2242,NBIMCC 8820,NCDO 2242,CIP 105419,NCIMB 702242,AS 1.2268,KCTC 3219,CCM 4988,HAMBI 112,BCRC 14668,NCAIM B.02018,NRRL B-41406,CGMCC 1.2268,VTT E-96663

@ref 20215

Last LPSN update 2025-12-16 09:47:10

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Bifidobacteriales

Family Bifidobacteriaceae

Genus Bifidobacterium

Species Bifidobacterium animalis subsp. animalis

Full scientific name Bifidobacterium animalis subsp. animalis (Mitsuoka 1969) Masco et al. 2004 (Approved Lists 1980)

BacDive ID	Other strains from **Bifidobacterium animalis subsp. animalis** (3)	Type strain
161178	B. animalis subsp. animalis JCM 11658, CGMCC 1.3003
165484	B. animalis subsp. animalis JCM 7117, ATCC 27674, LMG 18906
165486	B. animalis subsp. animalis JCM 7124, ATCC 27672

Morphology

Cell morphology

@ref	Gram stain	Motility	Confidence
116391	positive
125438			94.5

Colony morphology

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8529	BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)	Medium recipe at MediaDive	Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water
39288	MEDIUM 20 - for Anaerobic bacteria		Agar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
116391	CIP Medium 20	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8529	positive	growth	37	mesophilic
39288	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

8529

Oxygen toleranceanaerobe

Spore formation

@ref	Spore formation	Confidence
125438		91.971
125439		97.2

Murein

@ref	Murein short key	Type
8529	A11.21	A3alpha L-Lys(L-Orn)-L-Ala(L-Ser)-L-Ala₂

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
116391	17057 ChEBI	cellobiose	-	degradation
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
116391	17108 ChEBI	D-arabinose	+	degradation
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
116391	15824 ChEBI	D-fructose	+	degradation
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
116391	17634 ChEBI	D-glucose	+	degradation
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
116391	65327 ChEBI	D-xylose	+	degradation
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
116391	4853 ChEBI	esculin	-	hydrolysis
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
116391	17716 ChEBI	lactose	+	degradation
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
116391	17306 ChEBI	maltose	+	degradation
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	+	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
116391	17632 ChEBI	nitrate	-	reduction
116391	17632 ChEBI	nitrate	+	respiration
116391	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
116391	17814 ChEBI	salicin	-	degradation
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
116391	17992 ChEBI	sucrose	+	degradation
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
116391	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
116391	amylase	-
68382	beta-galactosidase	+	3.2.1.23	from API zym
116391	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
116391	caseinase	-	3.4.21.50
116391	catalase	-	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
116391	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
116391	gelatinase	-
116391	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
116391	lipase	-
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
116391	oxidase	-
116391	protease	-
68382	trypsin	-	3.4.21.4	from API zym
116391	tween esterase	-
116391	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
116391	-	-	+	+	-	+	-	+	-	-	+	+	+	+	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
8529	-	-	-	-	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	-	+	+/-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
8529	-	-	-	-	+	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	-	+	+	+	+	-	-	-	+	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Muridae (Mouse/Rat)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

Isolation

@ref	Sample type
8529	rat faeces
67770	Rat feces
116391	Animal, Rat, feces

Taxonmaps

69479

Global distribution of 16S sequence LC065042 (>99% sequence identity) for Bifidobacterium animalis subclade from Microbeatlas

Aquatic Samples	2336
Soil Samples	1194
Animal Samples	75452
Plant Samples	528
Total Samples	79510

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8529	1	Risk group (German classification) According to TRBA 466
116391	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM26071v1 assembly for Bifidobacterium animalis subsp. animalis ATCC 25527	complete	GCA_000260715	703613.4	2513237236	703613	99.17
67770	Bifani_sub.ani assembly for Bifidobacterium animalis subsp. animalis LMG 10508	contig	GCA_000741485	302912.4	2597490250	302912	76.02

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Bifidobacterium animalis ATCC 25527 16S rRNA-23S rRNA intergenic spacer	U09858	509	703613
20218	B.animalis DNA sequence for 16S rRNA	X70971	1459	703613
20218	Bifidobacterium animalis subsp. animalis strain CCUG 24606 16S ribosomal RNA gene, partial sequence	GQ487634	147	302912
20218	Bifidobacterium animalis gene for 16S rRNA, partial sequence, strain: JCM 1190	AB116277	481	28025
20218	Bifidobacterium animalis subsp. animalis gene for 16S rRNA, partial sequence, strain: JCM 1190	AB507070	652	302912
20218	Bifidobacterium animalis gene for 16S rRNA, partial sequence, strain: JCM 1190	D86185	1529	28025
20218	Bifidobacterium animalis subsp. animalis strain KCTC 3219 16S ribosomal RNA gene, partial sequence	GU361812	1493	302912
67770	Bifidobacterium animalis subsp. animalis gene for 16S ribosomal RNA, partial sequence, strain: JCM 1190	LC065042	1450	302912

GC content

@ref	GC-content (mol%)	Method
8529	60.1
67770	61.4	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Bifidobacterium animalis subsp. animalis ATCC 25527
PATRIC: 703613.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	97.20	no
125439	motility	BacteriaNetⓘ	no	70.50	no
125439	gram_stain	BacteriaNetⓘ	positive	79.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	84.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Bifidobacterium animalis subsp. animalis ATCC 25527 ATCC 25527
NCBI: GCA_000260715.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	84.88	no
125438	anaerobic	anaerobicⓘ	yes	85.22	yes
125438	aerobic	aerobicⓘ	no	94.21	yes
125438	spore-forming	spore-formingⓘ	no	91.97	no
125438	thermophilic	thermophileⓘ	no	94.00	yes
125438	flagellated	motile2+ⓘ	no	94.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Bifidobacterium and the intestinal mucus layer.	Gutierrez A, Pucket B, Engevik MA.	Microbiome Res Rep	10.20517/mrr.2023.37	2023
	Bifidobacterium beta-Glucosidase Activity and Fermentation of Dietary Plant Glucosides Is Species and Strain Specific.	Modrackova N, Vlkova E, Tejnecky V, Schwab C, Neuzil-Bunesova V.	Microorganisms	10.3390/microorganisms8060839	2020
Pathogenicity	An integrative understanding of the large metabolic shifts induced by antibiotics in critical illness.	Marfil-Sanchez A, Zhang L, Alonso-Pernas P, Mirhakkak M, Mueller M, Seelbinder B, Ni Y, Santhanam R, Busch A, Beemelmanns C, Ermolaeva M, Bauer M, Panagiotou G.	Gut Microbes	10.1080/19490976.2021.1993598	2021
Enzymology	Specific detection and analysis of a probiotic Bifidobacterium strain in infant feces.	Kok RG, de Waal A, Schut F, Welling GW, Weenk G, Hellingwerf KJ.	Appl Environ Microbiol	10.1128/aem.62.10.3668-3672.1996	1996
	Development of a Strain-Specific Detection and Quantification Method for Bifidobacterium animalis subsp. lactis HN019 Using WGS-SNP Analysis and qPCR.	Mao D, Zhao L, Zhao B, Xu H, Zhang Q.	Microorganisms	10.3390/microorganisms13071596	2025
	Cannabidiol reshapes the gut microbiome to promote endurance exercise in mice.	Chen S, Lee YB, Song MY, Lim C, Cho H, Shim HJ, Kim JS, Park BH, Kim JK, Bae EJ.	Exp Mol Med	10.1038/s12276-025-01404-5	2025
	Heterocyclic Antidepressants with Antimicrobial and Fungicide Activity.	Zolotareva D, Zazybin A, Belyankova Y, Bayazit S, Dauletbakov A, Seilkhanov T, Kemelbekov U, Aydemir M.	Molecules	10.3390/molecules30051102	2025
Genetics	Characterization of a Bifidobacterium animalis subsp. lactis reference strain based on ecology and transcriptomics.	Lugli GA, Argentini C, Tarracchini C, Mancabelli L, Viappiani A, Anzalone R, Angelini L, Alessandri G, Longhi G, Bianchi MG, Taurino G, Bussolati O, Milani C, Turroni F, Ventura M.	Appl Environ Microbiol	10.1128/aem.01080-24	2024
	Substrate recognition mode of a glycoside hydrolase family 42 beta-galactosidase from Bifidobacterium longum subspecies infantis (BiBga42A) revealed by crystallographic and mutational analyses.	Gotoh A, Hidaka M, Sakurama H, Nishimoto M, Kitaoka M, Sakanaka M, Fushinobu S, Katayama T.	Microbiome Res Rep	10.20517/mrr.2023.14	2023
Genetics	Draft Genome Sequences of Bifidobacterium animalis Consecutively Isolated from Healthy Japanese Individuals.	Tsukimi T, Watabe T, Tanaka K, Sato MP, Suzuki H, Tomita M, Fukuda S.	J Genomics	10.7150/jgen.38516	2020
	The Tetracycline Resistance Gene, tet(W) in Bifidobacterium animalis subsp. lactis Follows Phylogeny and Differs From tet(W) in Other Species.	Nohr-Meldgaard K, Struve C, Ingmer H, Agerso Y.	Front Microbiol	10.3389/fmicb.2021.658943	2021
	Bifidobacterium animalis: the missing link for the cancer-preventive effect of Gynostemma pentaphyllum.	Liao W, Khan I, Huang G, Chen S, Liu L, Leong WK, Li XA, Wu J, Wendy Hsiao WL.	Gut Microbes	10.1080/19490976.2020.1847629	2021
	Unveiling the inhibition mechanism of Clostridioides difficile by Bifidobacterium longum via multiomics approach.	Jo SH, Jeon HJ, Song WS, Lee JS, Kwon JE, Park JH, Kim YR, Kim MG, Baek JH, Kwon SY, Kim JS, Yang YH, Kim YG.	Front Microbiol	10.3389/fmicb.2023.1293149	2023
Phylogeny	Bifidobacterium animalis subsp. lactis ATCC 27673 is a genomically unique strain within its conserved subspecies.	Loquasto JR, Barrangou R, Dudley EG, Stahl B, Chen C, Roberts RF.	Appl Environ Microbiol	10.1128/aem.01777-13	2013
Pathogenicity	Co-Culture with Bifidobacterium catenulatum Improves the Growth, Gut Colonization, and Butyrate Production of Faecalibacterium prausnitzii: In Vitro and In Vivo Studies.	Kim H, Jeong Y, Kang S, You HJ, Ji GE.	Microorganisms	10.3390/microorganisms8050788	2020
Phylogeny	Dissecting the Evolutionary Development of the Species Bifidobacterium animalis through Comparative Genomics Analyses.	Lugli GA, Mancino W, Milani C, Duranti S, Mancabelli L, Napoli S, Mangifesta M, Viappiani A, Anzalone R, Longhi G, van Sinderen D, Ventura M, Turroni F.	Appl Environ Microbiol	10.1128/aem.02806-18	2019
	Influenza infection elicits an expansion of gut population of endogenous Bifidobacterium animalis which protects mice against infection.	Zhang Q, Hu J, Feng JW, Hu XT, Wang T, Gong WX, Huang K, Guo YX, Zou Z, Lin X, Zhou R, Yuan YQ, Zhang AD, Wei H, Cao G, Liu C, Chen LL, Jin ML.	Genome Biol	10.1186/s13059-020-02007-1	2020
Metabolism	Folate-producing bifidobacteria: metabolism, genetics, and relevance	D'Aimmo M, Satti M, Scarafile D, Modesto M, Pascarelli S, Biagini S, Luiselli D, Mattarelli P, Andlid T.	Microbiome Res Rep		2023
Pathogenicity	Unravelling the antimicrobial action of antidepressants on gut commensal microbes.	Ait Chait Y, Mottawea W, Tompkins TA, Hammami R.	Sci Rep	10.1038/s41598-020-74934-9	2020
	The influence of probiotic bacteria on the properties of Iranian white cheese	MAHMOUDI M, KHOSROWSHAHI ASL A, ZOMORODI S.	International journal of dairy technology.	10.1111/j.1471-0307.2012.00854.x	2012
Phylogeny	Comparative genomics of Bifidobacterium animalis subsp. lactis reveals a strict monophyletic bifidobacterial taxon.	Milani C, Duranti S, Lugli GA, Bottacini F, Strati F, Arioli S, Foroni E, Turroni F, van Sinderen D, Ventura M.	Appl Environ Microbiol	10.1128/aem.00984-13	2013
	Plant extract enhances the viability of Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus acidophilus in probiotic nonfat yogurt.	Michael M, Phebus RK, Schmidt KA.	Food Sci Nutr	10.1002/fsn3.189	2015
Phylogeny	Identification of species belonging to the Bifidobacterium genus by PCR-RFLP analysis of a hsp60 gene fragment.	Baffoni L, Stenico V, Strahsburger E, Gaggia F, Di Gioia D, Modesto M, Mattarelli P, Biavati B.	BMC Microbiol	10.1186/1471-2180-13-149	2013
Genetics	Genome-wide identification of small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and their regulation role in the adaption to gastrointestinal environment.	Zhu DQ, Liu F, Sun Y, Yang LM, Xin L, Meng XC.	PLoS One	10.1371/journal.pone.0117373	2015
	Prebiotic potential of Agave angustifolia Haw fructans with different degrees of polymerization.	Velazquez-Martinez JR, Gonzalez-Cervantes RM, Hernandez-Gallegos MA, Mendiola RC, Aparicio AR, Ocampo ML.	Molecules	10.3390/molecules190812660	2014
Genetics	Mobilome and Resistome Reconstruction from Genomes Belonging to Members of the Bifidobacterium Genus.	Mancino W, Lugli GA, Sinderen DV, Ventura M, Turroni F.	Microorganisms	10.3390/microorganisms7120638	2019
	Immunoreactive Proteins of Bifidobacterium longum ssp. longum CCM 7952 and Bifidobacterium longum ssp. longum CCDM 372 Identified by Gnotobiotic Mono-Colonized Mice Sera, Immune Rabbit Sera and Non-immune Human Sera.	Gorska S, Dylus E, Rudawska A, Brzozowska E, Srutkova D, Schwarzer M, Razim A, Kozakova H, Gamian A.	Front Microbiol	10.3389/fmicb.2016.01537	2016
	Oral co-administration of Lactiplantibacillus plantarum 16 and Lacticaseibacillus rhamnosus P118 improves host defense against influenza A virus infection.	Han M, Lu Q, Wang D, Zhou K, Jia C, Teng L, Hamuti A, Peng X, Hu Y, Li W, Yue M, Li Y.	J Virol	10.1128/jvi.00950-24	2024
Metabolism	Safety Evaluations of Bifidobacterium bifidum BGN4 and Bifidobacterium longum BORI.	Kim MJ, Ku S, Kim SY, Lee HH, Jin H, Kang S, Li R, Johnston TV, Park MS, Ji GE.	Int J Mol Sci	10.3390/ijms19051422	2018
Phylogeny	Comparative sequence analysis of the tuf and recA genes and restriction fragment length polymorphism of the internal transcribed spacer region sequences supply additional tools for discriminating Bifidobacterium lactis from Bifidobacterium animalis.	Ventura M, Zink R.	Appl Environ Microbiol	10.1128/aem.69.12.7517-7522.2003	2003
Enzymology	A new insight into the physiological role of bile salt hydrolase among intestinal bacteria from the genus Bifidobacterium.	Jarocki P, Podlesny M, Glibowski P, Targonski Z.	PLoS One	10.1371/journal.pone.0114379	2014
Enzymology	Detection of Bifidobacterium animalis subsp. lactis (Bb12) in the intestine after feeding of sows and their piglets.	Solano-Aguilar G, Dawson H, Restrepo M, Andrews K, Vinyard B, Urban JF.	Appl Environ Microbiol	10.1128/aem.00309-08	2008
	Comparative Genomics Revealed Genetic Diversity and Species/Strain-Level Differences in Carbohydrate Metabolism of Three Probiotic Bifidobacterial Species.	Odamaki T, Horigome A, Sugahara H, Hashikura N, Minami J, Xiao JZ, Abe F.	Int J Genomics	10.1155/2015/567809	2015
Genetics	Unveiling Genomic Diversity among Members of the Species Bifidobacterium pseudolongum, a Widely Distributed Gut Commensal of the Animal Kingdom.	Lugli GA, Duranti S, Albert K, Mancabelli L, Napoli S, Viappiani A, Anzalone R, Longhi G, Milani C, Turroni F, Alessandri G, Sela DA, van Sinderen D, Ventura M.	Appl Environ Microbiol	10.1128/aem.03065-18	2019
	Genetic basis of tetracycline resistance in Bifidobacterium animalis subsp. lactis.	Gueimonde M, Florez AB, van Hoek AH, Stuer-Lauridsen B, Stroman P, de los Reyes-Gavilan CG, Margolles A.	Appl Environ Microbiol	10.1128/aem.03096-09	2010
	A Gram-negative-selective antibiotic that spares the gut microbiome.	Munoz KA, Ulrich RJ, Vasan AK, Sinclair M, Wen PC, Holmes JR, Lee HY, Hung CC, Fields CJ, Tajkhorshid E, Lau GW, Hergenrother PJ.	Nature	10.1038/s41586-024-07502-0	2024
	Influence of the densities and nutritional components of bacterial colonies on the culture-enriched gut bacterial community structure.	Gu Y, Yan D, Wu M, Li M, Li P, Wang J, Chang Y, Yang F, Di S, Ni S, Yang M, Liu J.	AMB Express	10.1186/s13568-021-01240-6	2021
Metabolism	Diversity, ecology and intestinal function of bifidobacteria.	Bottacini F, Ventura M, van Sinderen D, O'Connell Motherway M.	Microb Cell Fact	10.1186/1475-2859-13-s1-s4	2014
Metabolism	Genomic overview and biological functions of exopolysaccharide biosynthesis in Bifidobacterium spp.	Hidalgo-Cantabrana C, Sanchez B, Milani C, Ventura M, Margolles A, Ruas-Madiedo P.	Appl Environ Microbiol	10.1128/aem.02977-13	2014
Metabolism	Transport of glucose by Bifidobacterium animalis subsp. lactis occurs via facilitated diffusion.	Briczinski EP, Phillips AT, Roberts RF.	Appl Environ Microbiol	10.1128/aem.01280-08	2008
	Gene structure and transcriptional organization of the dnaK operon of Bifidobacterium breve UCC 2003 and application of the operon in bifidobacterial tracing.	Ventura M, Zink R, Fitzgerald GF, van Sinderen D.	Appl Environ Microbiol	10.1128/aem.71.1.487-500.2005	2005
	Recent Development of Probiotic Bifidobacteria for Treating Human Diseases.	Chen J, Chen X, Ho CL.	Front Bioeng Biotechnol	10.3389/fbioe.2021.770248	2021
Genetics	Comparative genomics of the Bifidobacterium breve taxon.	Bottacini F, O'Connell Motherway M, Kuczynski J, O'Connell KJ, Serafini F, Duranti S, Milani C, Turroni F, Lugli GA, Zomer A, Zhurina D, Riedel C, Ventura M, van Sinderen D.	BMC Genomics	10.1186/1471-2164-15-170	2014
Enzymology	Diet supplementation with an organic acids-based formulation affects gut microbiota and expression of gut barrier genes in broilers.	Palamidi I, Mountzouris KC.	Anim Nutr	10.1016/j.aninu.2018.03.007	2018
	Occurrence and Diversity of CRISPR-Cas Systems in the Genus Bifidobacterium.	Briner AE, Lugli GA, Milani C, Duranti S, Turroni F, Gueimonde M, Margolles A, van Sinderen D, Ventura M, Barrangou R.	PLoS One	10.1371/journal.pone.0133661	2015
Enzymology	Specific identification and targeted characterization of Bifidobacterium lactis from different environmental isolates by a combined multiplex-PCR approach.	Ventura M, Reniero R, Zink R.	Appl Environ Microbiol	10.1128/aem.67.6.2760-2765.2001	2001
	Characterization of the groEL and groES loci in Bifidobacterium breve UCC 2003: genetic, transcriptional, and phylogenetic analyses.	Ventura M, Canchaya C, Zink R, Fitzgerald GF, van Sinderen D.	Appl Environ Microbiol	10.1128/aem.70.10.6197-6209.2004	2004
	The ClgR protein regulates transcription of the clpP operon in Bifidobacterium breve UCC 2003.	Ventura M, Zhang Z, Cronin M, Canchaya C, Kenny JG, Fitzgerald GF, van Sinderen D.	J Bacteriol	10.1128/jb.187.24.8411-8426.2005	2005
	Genetic characterization of the Bifidobacterium breve UCC 2003 hrcA locus.	Ventura M, Canchaya C, Bernini V, Del Casale A, Dellaglio F, Neviani E, Fitzgerald GF, van Sinderen D.	Appl Environ Microbiol	10.1128/aem.71.12.8998-9007.2005	2005
Phylogeny	Bifidobacterium lactis DSM 10140: identification of the atp (atpBEFHAGDC) operon and analysis of its genetic structure, characteristics, and phylogeny.	Ventura M, Canchaya C, van Sinderen D, Fitzgerald GF, Zink R.	Appl Environ Microbiol	10.1128/aem.70.5.3110-3121.2004	2004
Enzymology	Genetic and transcriptional organization of the clpC locus in Bifidobacterium breve UCC 2003.	Ventura M, Fitzgerald GF, van Sinderen D.	Appl Environ Microbiol	10.1128/aem.71.10.6282-6291.2005	2005
Metabolism	Genomic characterization and transcriptional studies of the starch-utilizing strain Bifidobacterium adolescentis 22L.	Duranti S, Turroni F, Lugli GA, Milani C, Viappiani A, Mangifesta M, Gioiosa L, Palanza P, van Sinderen D, Ventura M.	Appl Environ Microbiol	10.1128/aem.01993-14	2014
Enzymology	Species-specific oligonucleotide probes for five Bifidobacterium species detected in human intestinal microflora.	Yamamoto T, Morotomi M, Tanaka R.	Appl Environ Microbiol	10.1128/aem.58.12.4076-4079.1992	1992
Enzymology	Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis.	Satokari RM, Vaughan EE, Akkermans AD, Saarela M, de Vos WM.	Appl Environ Microbiol	10.1128/aem.67.2.504-513.2001	2001
Phylogeny	Quantification of different Eubacterium spp. in human fecal samples with species-specific 16S rRNA-targeted oligonucleotide probes.	Schwiertz A, Le Blay G, Blaut M.	Appl Environ Microbiol	10.1128/aem.66.1.375-382.2000	2000
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
	The effect of probiotics on functional constipation in adults: Double-blind, randomized, placebo-controlled study.	Mitelmao FCR, Bergamaschi CC, Gerenutti M, Hachel K, Silva MT, Balcao VM, Vila MMDC.	Medicine (Baltimore)	10.1097/md.0000000000024938	2021
Pathogenicity	Exploring the Ecology of Bifidobacteria and Their Genetic Adaptation to the Mammalian Gut.	Duranti S, Longhi G, Ventura M, van Sinderen D, Turroni F.	Microorganisms	10.3390/microorganisms9010008	2020
Phylogeny	Investigation of the evolutionary development of the genus Bifidobacterium by comparative genomics.	Lugli GA, Milani C, Turroni F, Duranti S, Ferrario C, Viappiani A, Mancabelli L, Mangifesta M, Taminiau B, Delcenserie V, van Sinderen D, Ventura M.	Appl Environ Microbiol	10.1128/aem.02004-14	2014
	Prevalence of Antibiotic Resistance Genes among Human Gut-Derived Bifidobacteria.	Duranti S, Lugli GA, Mancabelli L, Turroni F, Milani C, Mangifesta M, Ferrario C, Anzalone R, Viappiani A, van Sinderen D, Ventura M.	Appl Environ Microbiol	10.1128/aem.02894-16	2017
	Improved cloning vectors for bifidobacteria, based on the Bifidobacterium catenulatum pBC1 replicon.	Alvarez-Martin P, Belen Florez A, Margolles A, del Solar G, Mayo B.	Appl Environ Microbiol	10.1128/aem.00074-08	2008
Genetics	Comparative genomic and phylogenomic analyses of the Bifidobacteriaceae family.	Lugli GA, Milani C, Turroni F, Duranti S, Mancabelli L, Mangifesta M, Ferrario C, Modesto M, Mattarelli P, Jiri K, van Sinderen D, Ventura M.	BMC Genomics	10.1186/s12864-017-3955-4	2017
	Mixed-species genomic microarray analysis of fecal samples reveals differential transcriptional responses of bifidobacteria in breast- and formula-fed infants.	Klaassens ES, Boesten RJ, Haarman M, Knol J, Schuren FH, Vaughan EE, de Vos WM.	Appl Environ Microbiol	10.1128/aem.02492-08	2009
	Comparison of Real-Time PCR and Droplet Digital PCR for the Quantitative Detection of Lactiplantibacillus plantarum subsp. plantarum	Choi CH, Kim E, Yang SM, Kim DS, Suh SM, Lee GY, Kim HY.	Foods	10.3390/foods11091331	2022
	Designing primers and evaluation of the efficiency of propidium monoazide - Quantitative polymerase chain reaction for counting the viable cells of Lactobacillus gasseri and Lactobacillus salivarius.	Lai CH, Wu SR, Pang JC, Ramireddy L, Chiang YC, Lin CK, Tsen HY.	J Food Drug Anal	10.1016/j.jfda.2016.10.004	2017
	Alginate Alleviates Dextran Sulfate Sodium-Induced Colitis by Promoting Bifidobacterium animalis and Intestinal Hyodeoxycholic Acid Synthesis in Mice.	Pi Y, Zhang X, Wu Y, Wang Z, Bai Y, Liu X, Han D, Zhao J, Tobin I, Zhao J, Zhang G, Wang J.	Microbiol Spectr	10.1128/spectrum.02979-22	2022
Metabolism	Identification of plasmalogens in Bifidobacterium longum, but not in Bifidobacterium animalis.	Mawatari S, Sasuga Y, Morisaki T, Okubo M, Emura T, Fujino T.	Sci Rep	10.1038/s41598-019-57309-7	2020
	Methionine utilization by bifidobacteria: possible existence of a reverse transsulfuration pathway.	Wada M, Fukiya S, Suzuki A, Matsumoto N, Matsuo M, Yokota A.	Biosci Microbiota Food Health	10.12938/bmfh.2020-031	2021
Phylogeny	Rapid discrimination of Bifidobacterium animalis subspecies by matrix-assisted laser desorption ionization-time of flight mass spectrometry.	Ruiz-Moyano S, Tao N, Underwood MA, Mills DA.	Food Microbiol	10.1016/j.fm.2011.12.012	2012
	Comparison of Yacon (Smallanthus sonchifolius) Tuber with Commercialized Fructo-oligosaccharides (FOS) in Terms of Physiology, Fermentation Products and Intestinal Microbial Communities in Rats.	Utami NW, Sone T, Tanaka M, Nakatsu CH, Saito A, Asano K.	Biosci Microbiota Food Health	10.12938/bmfh.32.167	2013
Metabolism	Different effects of soybean protein and its derived peptides on the growth and metabolism of Bifidobacterium animalis subsp. animalis JCM 1190.	Li W, Li H, Zhang Y, He L, Zhang C, Liu X	Food Funct	10.1039/d1fo00480h	2021
Pathogenicity	Modulation of Proinflammatory Bacteria- and Lipid-Coupled Intracellular Signaling Pathways in a Transwell Triple Co-Culture Model by Commensal Bifidobacterium Animalis R101-8.	Ghadimi D, Nielsen A, Hassan MFY, Folster-Holst R, Ebsen M, Frahm SO, Rocken C, de Vrese M, Heller KJ	Antiinflamm Antiallergy Agents Med Chem	10.2174/1871523019999201029115618	2021
Genetics	Staying alive: growth and survival of Bifidobacterium animalis subsp. animalis under in vitro and in vivo conditions.	Egan M, Bottacini F, O'Connell Motherway M, Casey PG, Morrissey R, Melgar S, Faurie JM, Chervaux C, Smokvina T, van Sinderen D	Appl Microbiol Biotechnol	10.1007/s00253-018-9413-7	2018
Metabolism	The potential of bifidobacteria as a source of natural folate.	D'Aimmo MR, Mattarelli P, Biavati B, Carlsson NG, Andlid T	J Appl Microbiol	10.1111/j.1365-2672.2012.05261.x	2012
Genetics	Short communication: the complete genome sequence of Bifidobacterium animalis subspecies animalis ATCC 25527(T) and comparative analysis of growth in milk with B. animalis subspecies lactis DSM 10140(T).	Loquasto JR, Barrangou R, Dudley EG, Roberts RF	J Dairy Sci	10.3168/jds.2011-4499	2011
Pathogenicity	A dynamic model that simulates the human upper gastrointestinal tract for the study of probiotics.	Mainville I, Arcand Y, Farnworth ER	Int J Food Microbiol	10.1016/j.ijfoodmicro.2004.08.020	2005
Phylogeny	Rapid identification, differentiation, and proposed new taxonomic classification of Bifidobacterium lactis.	Ventura M, Zink R	Appl Environ Microbiol	10.1128/AEM.68.12.6429-6434.2002	2002
Phylogeny	Bifidobacterium lactis Meile et al. 1997 is a subjective synonym of Bifidobacterium animalis (Mitsuoka 1969) Scardovi and Trovatelli 1974.	Cai Y, Matsumoto M, Benno Y	Microbiol Immunol	10.1111/j.1348-0421.2000.tb02568.x	2000
Phylogeny	Characterization of fructose 6 phosphate phosphoketolases purified from Bifidobacterium species.	Grill JP, Crociani J, Ballongue J	Curr Microbiol	10.1007/BF00294634	1995
Phylogeny	Bifidobacterium canis sp. nov., a novel member of the Bifidobacterium pseudolongum phylogenetic group isolated from faeces of a dog (Canis lupus f. familiaris).	Neuzil-Bunesova V, Lugli GA, Modrackova N, Makovska M, Mrazek J, Mekadim C, Musilova S, Svobodova I, Spanek R, Ventura M, Killer J	Int J Syst Evol Microbiol	10.1099/ijsem.0.004378	2020
Phylogeny	Polyphasic taxonomic analysis of Bifidobacterium animalis and Bifidobacterium lactis reveals relatedness at the subspecies level: reclassification of Bifidobacterium animalis as Bifidobacterium animalis subsp. animalis subsp. nov. and Bifidobacterium lactis as Bifidobacterium animalis subsp. lactis subsp. nov.	Masco L, Ventura M, Zink R, Huys G, Swings J	Int J Syst Evol Microbiol	10.1099/ijs.0.03011-0	2004

References

#8529	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20104
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#39288	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116391	Collection of Institut Pasteur ; Curators of the CIP; CIP 105419
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Bifidobacterium animalis subsp. animalis (DSM 20104, ATCC 25527, CIP 105419)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Murein

Metabolite utilization

Metabolite production

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000260715

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media