Bifidobacterium thermacidophilum (DSM 15837, CGMCC AS 1.2282, JCM 11165)

Name: Bifidobacterium thermacidophilum (DSM 15837, CGMCC AS 1.2282, JCM 11165)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1735

Type strain

Strain Designation 36

Culture col. no. DSM 15837 CGMCC AS 1.2282 JCM 11165 36 VTT E-052869 4 more

NCBI tax ID(s) 246618 79262

Special Collections DSMZ JCM KCTC

History DSMZ <- JCM <- X. Dong; 36 JCM <-- X. Dong 36.

Links

Bifidobacterium thermacidophilum 36 is an anaerobe bacterium that was isolated from waste water of a bean-curd farm.

anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 14991

LMG 21395,AS 1.2282,ATCC 55326,JCM 11165,DSM 15837,CGMCC 1.2282,VTT E-052869

@ref 20215

Last LPSN update 2026-05-29 08:25:19

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Bifidobacteriales

Family Bifidobacteriaceae

Genus Bifidobacterium

Species Bifidobacterium thermacidophilum

Full scientific name Bifidobacterium thermacidophilum Dong et al. 2000

Morphology

Cell morphology

@ref	Motility	Confidence
125438		93

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6050	BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)		Medium recipe at MediaDive	Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
6050	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
6050	anaerobe
125439	facultative anaerobe	92.77

Spore formation

@ref	Spore formation	Confidence
125438		91.276
125439		99.082

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Agriculture	-
#Engineered	#Waste	#Wastewater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6050	waste water of a bean-curd farm	Beijing	China	CHN	Asia
67770	Wastewater of a bean-curd farm	Beijing	China	CHN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6050	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	Bifthe_sub.the assembly for Bifidobacterium thermacidophilum subsp. thermacidophilum LMG 21395	contig	GCA_000741455	79262.3	2600254984	79262	77.62
67770	ASM42642v1 assembly for Bifidobacterium thermacidophilum subsp. thermacidophilum DSM 15837	contig	GCA_000426425	1121111.3	2524614678	1121111	76.85
67770	DSM-15837 assembly for Bifidobacterium thermacidophilum subsp. thermacidophilum DSM 15837	contig	GCA_000771005	1121111.4	2675903354	1121111	71.63

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Bifidobacterium thermacidophilum gene for 16S rRNA, partial sequence, strain: JCM 11165	AB507157	660	246618
20218	Bifidobacterium thermacidophilum subsp. thermacidophilum strain JCM 11165 16S ribosomal RNA gene, partial sequence	GQ227716	308	79262
20218	Bifidobacterium thermacidophilum subsp. thermacidophilum strain DSM 15837 16S ribosomal RNA gene, partial sequence	GQ487647	139	79262
6050	Bifidobacterium thermophilum 16S rRNA gene, complete sequence	AB016246	1533	33905
67770	Bifidobacterium thermacidophilum subsp. thermacidophilum gene for 16S ribosomal RNA, partial sequence	AB437362	1512	79262
67770	Bifidobacterium thermacidophilum subsp. thermacidophilum gene for 16S ribosomal RNA, partial sequence, strain: JCM 11165	LC311741	1467	79262
124043	Bifidobacterium thermacidophilum subsp. thermacidophilum gene for 16S rRNA, partial sequence, strain: NBRC 106100.	AB682359	1456	79262

GC content

@ref	GC-content (mol%)	Method
6050	56.7
67770	57.7	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Bifthe_sub.the assembly for Bifidobacterium thermacidophilum subsp. thermacidophilum LMG 21395
BV-BRC: 79262.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	92.77	no
125439	gram_stain	BacteriaNetⓘ	positive	76.21	no
125439	motility	BacteriaNetⓘ	no	66.47	no
125439	spore_formation	BacteriaNetⓘ	no	99.08	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Bifidobacterium thermacidophilum subsp. thermacidophilum DSM 15837
PATRIC: 1121111.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.86	no
125438	anaerobic	anaerobicⓘ	yes	86.13	no
125438	spore-forming	spore-formingⓘ	no	91.28	no
125438	aerobic	aerobicⓘ	no	95.58	yes
125438	thermophilic	thermophileⓘ	no	92.49	yes
125438	flagellated	motile2+ⓘ	no	93.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Diversity, ecology and intestinal function of bifidobacteria.	Bottacini F, Ventura M, van Sinderen D, O'Connell Motherway M.	Microb Cell Fact	10.1186/1475-2859-13-s1-s4	2014
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
Genetics	Genomic Characteristics of Bifidobacterium thermacidophilum Pig Isolates and Wild Boar Isolates Reveal the Unique Presence of a Putative Mobile Genetic Element with tetW for Pig Farm Isolates.	Tsuchida S, Maruyama F, Ogura Y, Toyoda A, Hayashi T, Okuma M, Ushida K.	Front Microbiol	10.3389/fmicb.2017.01540	2017
	Comprehensive analysis of cell-associated fatty acids in Bifidobacterium strains.	Murakami R, Xiao JZ, Hara KY, Odamaki T, Kikukawa H.	Biosci Biotechnol Biochem	10.1093/bbb/zbaf144	2025
	Combined supplementation with Lactobacillus sp. and Bifidobacterium thermacidophilum isolated from Tibetan pigs improves growth performance, immunity, and microbiota composition in weaned piglets.	He Y, Liang J, Liu Y, Zhou X, Peng C, Long C, Huang P, Feng J, Zhang Z.	J Anim Sci	10.1093/jas/skad220	2023
	Exploring the gut microbiota of healthy captive Asian elephants from various locations in Yunnan, China.	Wang Y, Wang Y, Zhou J, Bao M, Shah T, Yang S, Zheng J, Li Q, Hou Y, Wang B, Yuan R.	Front Microbiol	10.3389/fmicb.2024.1403930	2024
	Investigation of the nutritional and functional roles of a microencapsulated blend of botanicals on intestinal health and growth of nursery pigs challenged with F18+Escherichia coli.	Garavito-Duarte Y, Bonetti A, Tugnoli B, Choi H, Piva A, Grilli E, Kim SW.	J Anim Sci	10.1093/jas/skaf047	2025
Metabolism	Search for bacterial alpha1,2-fucosyltransferases for whole-cell biosynthesis of 2'-fucosyllactose in recombinant Escherichia coli.	Seydametova E, Yu J, Shin J, Park Y, Kim C, Kim H, Yu SH, Park Y, Kweon DH.	Microbiol Res	10.1016/j.micres.2019.02.009	2019
	Dietary supplementation with fermented rapeseed and seaweed modulates parasite infections and gut microbiota in outdoor pigs	Bonde C, Drohse F, Budeyri Gokgoz N, Krych L, Nielsen D, Petersen H, Matthiesen R, Pedersen N, Geldhof P, Williams A, Thamsborg S, Mejer H.	Front Vet Sci		2025
	Impact of Lactobacillus- and Bifidobacterium-Based Direct-Fed Microbials on the Performance, Intestinal Morphology, and Fecal Bacterial Populations of Nursery Pigs.	Castillo Zuniga J, Fresno Rueda AM, Samuel RS, St-Pierre B, Levesque CL.	Microorganisms	10.3390/microorganisms12091786	2024
	Health benefits, antimicrobial activities, and potential applications of probiotics: A review.	Sepehr A, Miri ST, Aghamohammad S, Rahimirad N, Milani M, Pourshafie MR, Rohani M.	Medicine (Baltimore)	10.1097/md.0000000000032412	2024
	Nutritional and functional roles of beta-mannanase on intestinal health and growth of newly weaned pigs fed two different types of feeds.	Baker JT, Deng Z, Sokale A, Frederick B, Kim SW.	J Anim Sci	10.1093/jas/skae206	2024
Metabolism	Application of rumen microbes to enhance food waste hydrolysis in acidogenic leach-bed reactors.	Yan BH, Selvam A, Wong JW.	Bioresour Technol	10.1016/j.biortech.2014.03.085	2014
Genetics	Host-Diet Effect on the Metabolism of Bifidobacterium.	Satti M, Modesto M, Endo A, Kawashima T, Mattarelli P, Arita M.	Genes (Basel)	10.3390/genes12040609	2021
	Donated Human Milk as a Determinant Factor for the Gut Bifidobacterial Ecology in Premature Babies.	Arboleya S, Saturio S, Suarez M, Fernandez N, Mancabelli L, de Los Reyes-Gavilan CG, Ventura M, Solis G, Gueimonde M.	Microorganisms	10.3390/microorganisms8050760	2020
Phylogeny	Genetic and phenotypic diversity of Bifidobacterium thermacidophilum fecal isolates from newborns.	Kheadr E, Dabour N, von Ah U, Lacroix C, Meile L, Fliss I.	Can J Microbiol	10.1139/w07-101	2007
	Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in Lactobacillus amylovorus Isolates Derived from Wild Boars and Domestic Pigs.	Moravkova M, Kostovova I, Kavanova K, Pechar R, Stanek S, Brychta A, Zeman M, Kubasova T.	Microorganisms	10.3390/microorganisms11010103	2022
Enzymology	Increased amount of Bifidobacterium thermacidophilum and Megasphaera elsdenii in the colonic microbiota of pigs fed a swine dysentery preventive diet containing chicory roots and sweet lupine.	Molbak L, Thomsen LE, Jensen TK, Bach Knudsen KE, Boye M.	J Appl Microbiol	10.1111/j.1365-2672.2007.03430.x	2007
Phylogeny	Phylogenetic Analysis of the Bifidobacterium Genus Using Glycolysis Enzyme Sequences.	Brandt K, Barrangou R.	Front Microbiol	10.3389/fmicb.2016.00657	2016
Phylogeny	Investigation of the evolutionary development of the genus Bifidobacterium by comparative genomics.	Lugli GA, Milani C, Turroni F, Duranti S, Ferrario C, Viappiani A, Mancabelli L, Mangifesta M, Taminiau B, Delcenserie V, van Sinderen D, Ventura M.	Appl Environ Microbiol	10.1128/aem.02004-14	2014
	Effect of Bifidobacterium thermacidophilum probiotic feeding on enterohemorrhagic Escherichia coli O157:H7 infection in BALB/c mice.	Gagnon M, Kheadr EE, Dabour N, Richard D, Fliss I.	Int J Food Microbiol	10.1016/j.ijfoodmicro.2006.04.041	2006
Phylogeny	Identification of species belonging to the Bifidobacterium genus by PCR-RFLP analysis of a hsp60 gene fragment.	Baffoni L, Stenico V, Strahsburger E, Gaggia F, Di Gioia D, Modesto M, Mattarelli P, Biavati B.	BMC Microbiol	10.1186/1471-2180-13-149	2013
Metabolism	Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract.	Kim PI, Jung MY, Chang YH, Kim S, Kim SJ, Park YH.	Appl Microbiol Biotechnol	10.1007/s00253-006-0741-7	2007
	The effects of prebiotics on growth performance and in vitro immune biomarkers in weaned pigs.	San Andres JV, Mastromano GA, Li Y, Tran H, Bundy JW, Miller PS, Burkey TE.	Transl Anim Sci	10.1093/tas/txz129	2019
Genetics	Deciphering the Bifidobacterial Populations within the Canine and Feline Gut Microbiota.	Alessandri G, Milani C, Mancabelli L, Longhi G, Anzalone R, Lugli GA, Duranti S, Turroni F, Ossiprandi MC, van Sinderen D, Ventura M.	Appl Environ Microbiol	10.1128/aem.02875-19	2020
Genetics	Comparative genomic and phylogenomic analyses of the Bifidobacteriaceae family.	Lugli GA, Milani C, Turroni F, Duranti S, Mancabelli L, Mangifesta M, Ferrario C, Modesto M, Mattarelli P, Jiri K, van Sinderen D, Ventura M.	BMC Genomics	10.1186/s12864-017-3955-4	2017
	PCR and real-time PCR primers developed for detection and identification of Bifidobacterium thermophilum in faeces.	Mathys S, Lacroix C, Mini R, Meile L.	BMC Microbiol	10.1186/1471-2180-8-179	2008
	Pig manure contamination marker selection based on the influence of biological treatment on the dominant fecal microbial groups.	Marti R, Dabert P, Pourcher AM.	Appl Environ Microbiol	10.1128/aem.02791-08	2009
Phylogeny	Classification of a moderately oxygen-tolerant isolate from baby faeces as Bifidobacterium thermophilum.	von Ah U, Mozzetti V, Lacroix C, Kheadr EE, Fliss I, Meile L.	BMC Microbiol	10.1186/1471-2180-7-79	2007
Enzymology	The Sortase-Dependent Fimbriome of the Genus Bifidobacterium: Extracellular Structures with Potential To Modulate Microbe-Host Dialogue.	Milani C, Mangifesta M, Mancabelli L, Lugli GA, Mancino W, Viappiani A, Faccini A, van Sinderen D, Ventura M, Turroni F.	Appl Environ Microbiol	10.1128/aem.01295-17	2017
Phylogeny	Exploring Vertical Transmission of Bifidobacteria from Mother to Child.	Milani C, Mancabelli L, Lugli GA, Duranti S, Turroni F, Ferrario C, Mangifesta M, Viappiani A, Ferretti P, Gorfer V, Tett A, Segata N, van Sinderen D, Ventura M.	Appl Environ Microbiol	10.1128/aem.02037-15	2015
	Inactivation of adhesion and invasion of food-borne Listeria monocytogenes by bacteriocin-producing Bifidobacterium strains of human origin.	Moroni O, Kheadr E, Boutin Y, Lacroix C, Fliss I.	Appl Environ Microbiol	10.1128/aem.00928-06	2006
Phylogeny	Species identification of genus Bifidobacterium based on partial HSP60 gene sequences and proposal of Bifidobacterium thermacidophilum subsp. porcinum subsp. nov.	Zhu L, Li W, Dong X.	Int J Syst Evol Microbiol	10.1099/ijs.0.02617-0	2003
Phylogeny	Bifidobacterium thermacidophilum sp. nov., isolated from an anaerobic digester.	Dong X, Xin Y, Jian W, Liu X, Ling D.	Int J Syst Evol Microbiol	10.1099/00207713-50-1-119	2000

References

#6050	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15837
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Bifidobacterium thermacidophilum (DSM 15837, CGMCC AS 1.2282, JCM 11165)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000741455

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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