Name: Vibrio neptunius DSM 17183
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17306

Type strain:

Species: Vibrio neptunius

Culture col. no.: DSM 17183, CAIM 532, CIP 108274, KCTC 12702, LMG 20536, KCTC 12123, INCO 17

Strain history: CIP <- 2004, CAIM

NCBI tax ID(s): 170651 (species)

SI-ID 19354

LMG 20536, KCTC 12123, DSM 17183, CIP 108274, KCTC 12702

Section

Vibrio neptunius DSM 17183 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from bivalve larvae .

Gram-negative
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	"Vibrionales"
	Family	*Vibrionaceae*
	Genus	*Vibrio*
	Species	**Vibrio neptunius**
	Full Scientific Name (LPSN)	Vibrio neptunius Thompson et al. 2003

Information on morphological and physiological properties Morphology

[Ref.: #67771]	Gram stain	negative
[Ref.: #121273]	Gram stain	negative

[Ref.: #121273]	Cell shape	rod-shaped

[Ref.: #121273]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6716]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #6716]

Culture medium growth

[Ref.: #6716]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6716]

Culture medium composition

expand / minimize

[Ref.: #36713]

Culture medium

Marine agar (MA)

[Ref.: #36713]

Culture medium growth

[Ref.: #36713]

Culture medium composition

expand / minimize

[Ref.: #121273]

Culture medium

CIP Medium 13

[Ref.: #121273]

Culture medium growth

[Ref.: #121273]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #6716]		growth	28 °C
[Ref.: #36713]		growth	25 °C
[Ref.: #67771]		growth	28 °C
[Ref.: #121273]		growth	15-37 °C
[Ref.: #121273]	no	growth	5 °C
[Ref.: #121273]	no	growth	41 °C

[Ref.: #6716]	Temperature range	mesophilic
[Ref.: #36713]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic
[Ref.: #121273]	Temperature range	psychrophilic
[Ref.: #121273]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]	Oxygen tolerance	aerobe
[Ref.: #121273]	Oxygen tolerance	facultative anaerobe

	Salt	Tested relation		Salt conc.
[Ref.: #121273]	NaCl		growth	2-8	%
[Ref.: #121273]	NaCl	no	growth	0	%
[Ref.: #121273]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #121273]	citrate ChEBI	-	carbon source
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #121273]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #121273]	glucose ChEBI	+	fermentation
[Ref.: #121273]	glucose ChEBI	-	degradation
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #121273]	lactose ChEBI	-	fermentation
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #121273]	nitrate ChEBI	-	reduction
[Ref.: #121273]	nitrate ChEBI	+	respiration
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #121273]	nitrite ChEBI	-	reduction
[Ref.: #121273]	sodium thiosulfate ChEBI	-	builds gas from
[Ref.: #68369]	tryptophan ChEBI	+	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #121273]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #121273]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	yes	* from API 20NE

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #121273]	acetoin ChEBI	-
[Ref.: #121273]	glucose ChEBI		-
[Ref.: #68369]	indole ChEBI			+	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121273]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121273]	amylase	+
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #121273]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121273]	caseinase	+	3.4.21.50
[Ref.: #121273]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #121273]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #121273]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #121273]	gelatinase	+
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #121273]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121273]	lipase	+
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121273]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121273]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121273]	oxidase	+
[Ref.: #121273]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #121273]	protease	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #121273]	tryptophan deaminase	-
[Ref.: #121273]	tween esterase	+
[Ref.: #121273]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #6716]	23329	-	+	+	-	-	-	+	-	+	-	+	-	+	-	-	+	-	+	+	-	+

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121273]	2685	not determinedn.d.	+	+	-	+	+	-	-	+	-	+	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	+	-	-	-	-	-	+	-	-	+	+	+	+	+	+	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121273]	11046	-	+	+	+	-	+	-	-	+	-	+	+	-	-	-	-	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6716]	Sample type/isolated from	bivalve larvae (Nodipecten nodosus)
[Ref.: #6716]	Host species	Nodipecten nodosus
[Ref.: #6716]	Geographic location (country and/or sea, region)	Florianopolis
[Ref.: #6716]	Country	Brazil
[Ref.: #6716]	Country ISO 3 Code	BRA
[Ref.: #6716]	Continent	Middle and South America

[Ref.: #67771]	Sample type/isolated from	From larvae of the bivalve Nodipecten nodosus in the south of Brazil
[Ref.: #67771]	Country	Brazil
[Ref.: #67771]	Country ISO 3 Code	BRA
[Ref.: #67771]	Continent	Middle and South America

[Ref.: #121273]	Sample type/isolated from	Animal, Healthy Nodipecten, nodosus larvae
[Ref.: #121273]	Geographic location (country and/or sea, region)	Florianopolis
[Ref.: #121273]	Country	Brazil
[Ref.: #121273]	Country ISO 3 Code	BRA
[Ref.: #121273]	Continent	Middle and South America
[Ref.: #121273]	Isolation date	1998
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Host	#Invertebrates (Other)	#Mollusca

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JN039149 (>99% sequence identity) for Vibrio from Microbeatlas

Aquatic Samples	3721
Soil Samples	27
Animal Samples	50
Plant Samples	3
Total Samples	3801

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6716]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121273]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6716]	Vibrio sp. LMG 20536 16S rRNA gene, strain LMG 20536	AJ316171	1468	ENA	170651 tax ID
[Ref.: #20218]	Vibrio neptunius strain LMG 20536 16S ribosomal RNA gene, partial sequence	JN039149	1468	ENA	170651 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Vibrio neptunius KCTC 12702	GCA_019339365	complete	GenBank	170651 tax ID	*
[Ref.: #66792]	Vibrio neptunius strain KCTC 12702	170651.32	complete	PATRIC	170651 tax ID	*

[Ref.: #67771]

GC-content

46.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Vibrio neptunius KCTC 12702 NCBI: GCA_019339365.1
[Ref.: #69480]	flagellated	yes	92.468	no
[Ref.: #69480]	gram-positive	no	98.808	yes
[Ref.: #69480]	anaerobic	no	96.882	yes
[Ref.: #69480]	aerobic	yes	82.192	yes
[Ref.: #69480]	halophile	yes	65.535	no
[Ref.: #69480]	spore-forming	no	94.984	no
[Ref.: #69480]	motile	yes	91.718	no
[Ref.: #69480]	thermophile	no	99.678	yes
[Ref.: #69480]	glucose-util	yes	88.678	yes
[Ref.: #69480]	glucose-ferment	yes	85.686	no

Availability in culture collections External links

[Ref.: #6716]

Culture collection no.

DSM 17183, CAIM 532, CIP 108274, KCTC 12702, LMG 20536, KCTC 12123, INCO 17

[Ref.: #86306]

SI-ID 19354

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Vibrio variabilis sp. nov. and Vibrio maritimus sp. nov., isolated from Palythoa caribaeorum.	Chimetto LA, Cleenwerck I, Moreira APB, Brocchi M, Willems A, De Vos P, Thompson FL	Int J Syst Evol Microbiol	10.1099/ijs.0.026997-0	2011	*
Phylogeny	Usefulness of a real-time PCR platform for G+C content and DNA-DNA hybridization estimations in vibrios.	Moreira APB, Pereira N, Thompson FL	Int J Syst Evol Microbiol	10.1099/ijs.0.023606-0	2010	*
Phylogeny	Vibrio neptunius sp. nov., Vibrio brasiliensis sp. nov. and Vibrio xuii sp. nov., isolated from the marine aquaculture environment (bivalves, fish, rotifers and shrimps).	Thompson FL, Li Y, Gomez-Gil B, Thompson CC, Hoste B, Vandemeulebroecke K, Rupp GS, Pereira A, De Bem MM, Sorgeloos P, Swings J	Int J Syst Evol Microbiol	10.1099/ijs.0.02447-0	2003	*
Phylogeny	Vibrio ostreae sp. nov., a novel gut bacterium isolated from a Yellow Sea oyster.	Muhammad N, Nguyen TTH, Lee YJ, Ko J, Avila F, Kim SG	Int J Syst Evol Microbiol	10.1099/ijsem.0.005586	2022	*

References

#6716

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17183

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36713 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#86306

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID19354.1 )

#121273 Collection of Institut Pasteur ; Curators of the CIP; CIP 108274
* These data were automatically processed and therefore are not curated

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Search for species Vibrio neptunius in external resources:
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Vibrio neptunius KCTC 12702

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