Name: Megasphaera elsdenii LC1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17130

Type strain:

Species: Megasphaera elsdenii

Strain Designation: LC1

Culture col. no.: DSM 20460, ATCC 25940, NCIB 8927, CCUG 64197, JCM 1772, CCUG 6199, CECT 390, CGMCC 1.2720, KCTC 5187, NCFB 2263, NCIMB 8927, VTT E-84221, CIP 106852, AIP 10100

Strain history: CIP <- 2000, JP Carlier, Institut Pasteur, Paris, France: strain AIP 10100 <- NCIB <- S.R. Elsden: strain LC1

NCBI tax ID(s): 1064535 (strain), 907 (species)

SI-ID 38727

ATCC 25940, NCIB 8927, CCUG 6199, DSM 20460, JCM 1772, NCIMB 8927, NCDO2263, NCIMB 702264, NCDO2264, NCDO2331, NCIMB 702331, CECT 390, NCFB 2263, NCTC 8927, VTT E-84221, CIP 106852, KCTC 5187, CGMCC 1.2720, LMG 25663, CCUG 64197

Other strains from Megasphaera elsdenii

M. elsdenii PG-130-P53-9, DSM 100961
M. elsdenii WCA-386-APC-2A, DSM 106891

Section

Megasphaera elsdenii LC1 is an anaerobe, mesophilic, Gram-negative human pathogen that was isolated from rumen of sheep.

Gram-negative
anaerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Negativicutes*
	Order	*Veillonellales*
	Family	*Veillonellaceae*
	Genus	*Megasphaera*
	Species	**Megasphaera elsdenii**
	Full Scientific Name (LPSN)	Megasphaera elsdenii (Gutierrez et al. 1959) Rogosa 1971 (Approved Lists 1980)

Synonym

"Peptostreptococcus elsdenii"

Information on morphological and physiological properties Morphology

[Ref.: #119020]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.816

[Ref.: #119020]	Cell shape	coccus-shaped

[Ref.: #119020]	Motility	no

[Ref.: #8831]	Incubation period	1-2 days

[Ref.: #63098]	Incubation period	1 day

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8831]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #8831]

Culture medium growth

[Ref.: #8831]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8831]

Culture medium composition

expand / minimize

[Ref.: #34386]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #34386]

Culture medium growth

[Ref.: #34386]

Culture medium composition

expand / minimize

[Ref.: #119020]

Culture medium

CIP Medium 20

[Ref.: #119020]

Culture medium growth

[Ref.: #119020]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8831]	growth	37 °C
[Ref.: #34386]	growth	37 °C
[Ref.: #63098]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #8831]	Temperature range	mesophilic
[Ref.: #34386]	Temperature range	mesophilic
[Ref.: #63098]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8831]	Oxygen tolerance	anaerobe
[Ref.: #63098]	Oxygen tolerance	anaerobe
[Ref.: #119020]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.933

[Ref.: #8831]	Murein short key	A31
[Ref.: #8831]	Murein types	A1gamma m-Dpm-direct

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68367]	cellobiose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-glucose ChEBI	+	builds acid from	* from API 20A
[Ref.: #68367]	D-mannitol ChEBI	+	builds acid from	* from API 20A
[Ref.: #68367]	D-mannose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68367]	D-xylose ChEBI	-	builds acid from	* from API 20A
[Ref.: #119020]	esculin ChEBI	-	hydrolysis
[Ref.: #68367]	esculin ChEBI	-	hydrolysis	* from API 20A
[Ref.: #68367]	gelatin ChEBI	-	hydrolysis	* from API 20A
[Ref.: #68367]	glycerol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	L-arabinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68367]	L-rhamnose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	lactose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	maltose ChEBI	+	builds acid from	* from API 20A
[Ref.: #68367]	melezitose ChEBI	-	builds acid from	* from API 20A
[Ref.: #119020]	nitrate ChEBI	-	reduction
[Ref.: #119020]	nitrate ChEBI	+	respiration
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #119020]	nitrite ChEBI	-	reduction
[Ref.: #68367]	raffinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68367]	sorbitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	sucrose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	trehalose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	tryptophan ChEBI	-	energy source	* from API 20A, API rID32A
[Ref.: #68367]	urea ChEBI	-	hydrolysis	* from API 20A, API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #119020]		metronidazole ChEBI	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #119020]		indole ChEBI	no
[Ref.: #68367]		indole ChEBI	no	* from API 20A, API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A, API 20A

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A, API zym
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A, API zym
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A, API zym
[Ref.: #68380]	alpha-glucosidase	-	3.2.1.20	* from API rID32A, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #119020]	amylase	-
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #119020]	beta-galactosidase	-	3.2.1.23
[Ref.: #68380]	beta-galactosidase	-	3.2.1.23	* from API rID32A, API zym
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A, API 20A, API zym
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A, API zym
[Ref.: #119020]	caseinase	-	3.4.21.50
[Ref.: #119020]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #119020]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #119020]	gelatinase	-
[Ref.: #68367]	gelatinase	-		* from API 20A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #119020]	lecithinase	-
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A, API zym
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #119020]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #119020]	oxidase	-
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #119020]	protease	-
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #119020]	tween esterase	-
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #119020]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A, API 20A
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Indole productionIND	Urease/urea hydrolysisURE	Acid from D-glucoseGLU	Acid from D-mannitolMAN	Acid from lactoseLAC	Acid from sucroseSAC	Acid from maltoseMAL	Acid from salicinSAL	Acid from D-xyloseXYL	Acid from L-arabinoseARA	Gelatinase/gelatin hydrolysisGEL	Esculin hydrolysis/beta-glucosidaseESC	Acid from glycerolGLY	Acid from cellobioseCEL	Acid from D-mannoseMNE	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from sorbitolSOR	Acid from L-rhamnoseRHA	Acid from trehaloseTRE	CatalaseCAT	Spores presentSPOR	GramGRAM	Morphology coccus="+" rod="-"COCC
[Ref.: #8831]	2038	not determinedn.d.	-	+	+	-	-	+	+/-	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #8831]	2037	-	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #8831]	13041	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119020]	9861	-	-	+	+	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	gluconeogenesis	100	8 of 8
[Ref.: #66794]	sulfopterin metabolism	100	4 of 4
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	ribulose monophosphate pathway	100	2 of 2
[Ref.: #66794]	reductive acetyl coenzyme A pathway	100	7 of 7
[Ref.: #66794]	aminopropanol phosphate biosynthesis	100	2 of 2
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	acetoin degradation	100	3 of 3
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	threonine metabolism	90	9 of 10
[Ref.: #66794]	CO2 fixation in Crenarchaeota	88.89	8 of 9
[Ref.: #66794]	serine metabolism	88.89	8 of 9
[Ref.: #66794]	valine metabolism	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	isoleucine metabolism	87.5	7 of 8
[Ref.: #66794]	C4 and CAM-carbon fixation	87.5	7 of 8
[Ref.: #66794]	palmitate biosynthesis	86.36	19 of 22
[Ref.: #66794]	propanol degradation	85.71	6 of 7
[Ref.: #66794]	tetrahydrofolate metabolism	85.71	12 of 14
[Ref.: #66794]	vitamin B12 metabolism	85.29	29 of 34
[Ref.: #66794]	vitamin B1 metabolism	84.62	11 of 13
[Ref.: #66794]	alanine metabolism	82.76	24 of 29
[Ref.: #66794]	glutamate and glutamine metabolism	82.14	23 of 28
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	methylglyoxal degradation	80	4 of 5
[Ref.: #66794]	flavin biosynthesis	80	12 of 15
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	phenylacetate degradation (aerobic)	80	4 of 5
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	lipid A biosynthesis	77.78	7 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	77.78	7 of 9
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	biotin biosynthesis	75	3 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	pentose phosphate pathway	72.73	8 of 11
[Ref.: #66794]	citric acid cycle	71.43	10 of 14
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	pyrimidine metabolism	71.11	32 of 45
[Ref.: #66794]	starch degradation	70	7 of 10
[Ref.: #66794]	Entner Doudoroff pathway	70	7 of 10
[Ref.: #66794]	purine metabolism	69.15	65 of 94
[Ref.: #66794]	NAD metabolism	66.67	12 of 18
[Ref.: #66794]	methane metabolism	66.67	2 of 3
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	aspartate and asparagine metabolism	66.67	6 of 9
[Ref.: #66794]	glycolysis	64.71	11 of 17
[Ref.: #66794]	lipid metabolism	64.52	20 of 31
[Ref.: #66794]	heme metabolism	64.29	9 of 14
[Ref.: #66794]	ketogluconate metabolism	62.5	5 of 8
[Ref.: #66794]	glycine betaine biosynthesis	60	3 of 5
[Ref.: #66794]	hydrogen production	60	3 of 5
[Ref.: #66794]	glycogen metabolism	60	3 of 5
[Ref.: #66794]	propionate fermentation	60	6 of 10
[Ref.: #66794]	tryptophan metabolism	57.89	22 of 38
[Ref.: #66794]	d-mannose degradation	55.56	5 of 9
[Ref.: #66794]	nitrate assimilation	55.56	5 of 9
[Ref.: #66794]	cysteine metabolism	55.56	10 of 18
[Ref.: #66794]	histidine metabolism	55.17	16 of 29
[Ref.: #66794]	oxidative phosphorylation	54.95	50 of 91
[Ref.: #66794]	proline metabolism	54.55	6 of 11
[Ref.: #66794]	methionine metabolism	53.85	14 of 26
[Ref.: #66794]	leucine metabolism	53.85	7 of 13
[Ref.: #66794]	degradation of sugar acids	52	13 of 25
[Ref.: #66794]	selenocysteine biosynthesis	50	3 of 6
[Ref.: #66794]	arginine metabolism	50	12 of 24
[Ref.: #66794]	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	dTDPLrhamnose biosynthesis	50	4 of 8
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	lysine metabolism	50	21 of 42
[Ref.: #66794]	glycolate and glyoxylate degradation	50	3 of 6
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	50	6 of 12
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	lactate fermentation	50	2 of 4
[Ref.: #66794]	sulfate reduction	46.15	6 of 13
[Ref.: #66794]	isoprenoid biosynthesis	46.15	12 of 26
[Ref.: #66794]	degradation of hexoses	44.44	8 of 18
[Ref.: #66794]	tyrosine metabolism	42.86	6 of 14
[Ref.: #66794]	gallate degradation	40	2 of 5
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	urea cycle	38.46	5 of 13
[Ref.: #66794]	phenylpropanoid biosynthesis	38.46	5 of 13
[Ref.: #66794]	metabolism of disaccharids	36.36	4 of 11
[Ref.: #66794]	vitamin B6 metabolism	36.36	4 of 11
[Ref.: #66794]	degradation of pentoses	35.71	10 of 28
[Ref.: #66794]	non-pathway related	34.21	13 of 38
[Ref.: #66794]	pantothenate biosynthesis	33.33	2 of 6
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	octane oxidation	33.33	1 of 3
[Ref.: #66794]	ascorbate metabolism	31.82	7 of 22
[Ref.: #66794]	degradation of sugar alcohols	31.25	5 of 16
[Ref.: #66794]	polyamine pathway	30.43	7 of 23
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	phenol degradation	30	6 of 20
[Ref.: #66794]	4-hydroxyphenylacetate degradation	30	3 of 10
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	d-xylose degradation	27.27	3 of 11
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	vitamin E metabolism	25	1 of 4
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	23.08	3 of 13
[Ref.: #66794]	4-hydroxymandelate degradation	22.22	2 of 9
[Ref.: #66794]	glutathione metabolism	21.43	3 of 14
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8831]	Sample type/isolated from	rumen of sheep

[Ref.: #63098]	Sample type/isolated from	Rumen of sheep

[Ref.: #67770]	Sample type/isolated from	Rumen of sheep

[Ref.: #119020]	Sample type/isolated from	Animal, Sheep, rumen
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Caprinae (Sheep/Goat)
#Host Body-Site	#Organ	#Rumen

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC036319 (>99% sequence identity) for Megasphaera elsdenii subclade from Microbeatlas

Aquatic Samples	989
Soil Samples	544
Animal Samples	27646
Plant Samples	254
Total Samples	29433

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8831]	Pathogenicity (human)	yes, in single cases
[Ref.: #8831]	Pathogenicity (animal)	yes, in single cases
[Ref.: #8831]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119020]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Megasphaera elsdenii gene for 16S ribosomal RNA, partial sequence, strain: JCM 1772	LC036319	1525	ENA	907 tax ID
[Ref.: #20218]	Megasphaera elsdenii gene for 16S rRNA, partial sequence, strain: DSM 20460, type A	AB609704	1174	ENA	1064535 tax ID	*
[Ref.: #20218]	Megasphaera elsdenii gene for 16S rRNA, partial sequence, strain: DSM 20460, type B	AB609705	1174	ENA	1064535 tax ID	*
[Ref.: #20218]	Megasphaera elsdenii ATCC 25940 16S ribosomal RNA gene, partial sequence	U95027	1426	ENA	1064535 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Megasphaera elsdenii DSM 20460 ATCC 25940	GCA_003010495	complete	GenBank	1064535 tax ID	*
[Ref.: #66792]	Megasphaera elsdenii DSM 20460	GCA_000283495	chromosome	GenBank	1064535 tax ID	*
[Ref.: #66792]	Megasphaera elsdenii DSM 20460	907.4	complete	PATRIC	1064535 tax ID	*
[Ref.: #66792]	Megasphaera elsdenii DSM 20460 strain ATCC 25940	1064535.9	complete	PATRIC	1064535 tax ID	*
[Ref.: #66792]	Megasphaera elsdenii DSM 20460	2511231071	draft	JGI IMG/M	1064535 tax ID	*
[Ref.: #66792]	Megasphaera elsdenii ATCC 25940	2843666722	complete	JGI IMG/M	1064535 tax ID	*

[Ref.: #8831]

GC-content

52.7 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Megasphaera elsdenii DSM 20460 ATCC 25940 NCBI: GCA_003010495.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Megasphaera elsdenii DSM 20460 ATCC 25940 NCBI: GCA_003010495.1
[Ref.: #69480]	flagellated	no	96.85	no
[Ref.: #69480]	gram-positive	no	94.635	no
[Ref.: #69480]	anaerobic	yes	99.533	yes
[Ref.: #69480]	aerobic	no	97.673	yes
[Ref.: #69480]	halophile	no	88.306	no
[Ref.: #69480]	spore-forming	no	92.621	no
[Ref.: #69480]	glucose-util	yes	70.258	no
[Ref.: #69480]	thermophile	no	97.71	yes
[Ref.: #69480]	motile	no	94.414	no
[Ref.: #69480]	glucose-ferment	yes	57.448	no

Availability in culture collections External links

[Ref.: #8831]

Culture collection no.

DSM 20460, ATCC 25940, NCIB 8927, CCUG 64197, JCM 1772, CCUG 6199, CECT 390, CGMCC 1.2720, KCTC 5187, NCFB 2263, NCIMB 8927, VTT E-84221, CIP 106852, AIP 10100

[Ref.: #86129]

SI-ID 38727

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequences of Two Megasphaera elsdenii Strains, NCIMB 702410 and ATCC 25940.	Hatmaker EA, Klingeman DM, O'Dell KB, Riley LA, Papanek B, Guss AM	Microbiol Resour Announc	10.1128/MRA.01430-18	2019	*
Metabolism	Valerate production by Megasphaera elsdenii isolated from pig feces.	Yoshikawa S, Araoka R, Kajihara Y, Ito T, Miyamoto H, Kodama H	J Biosci Bioeng	10.1016/j.jbiosc.2017.12.016	2018	*
Phylogeny	Preferential isolation of Megasphaera elsdenii from pig feces.	Kajihara Y, Yoshikawa S, Cho Y, Ito T, Miyamoto H, Kodama H	Anaerobe	10.1016/j.anaerobe.2017.08.013	2017	*
Phylogeny	Megasphaera hexanoica sp. nov., a medium-chain carboxylic acid-producing bacterium isolated from a cow rumen.	Jeon BS, Kim S, Sang BI	Int J Syst Evol Microbiol	10.1099/ijsem.0.001888	2017	*
Phylogeny	Megasphaera indica sp. nov., an obligate anaerobic bacteria isolated from human faeces.	Lanjekar VB, Marathe NP, Ramana VV, Shouche YS, Ranade DR	Int J Syst Evol Microbiol	10.1099/ijs.0.059816-0	2014	*
Metabolism	Comparative genome analysis of Megasphaera sp. reveals niche specialization and its potential role in the human gut.	Shetty SA, Marathe NP, Lanjekar V, Ranade D, Shouche YS	PLoS One	10.1371/journal.pone.0079353	2013	*
Metabolism	Intraruminal administration of Megasphaera elsdenii modulated rumen fermentation profile in mid-lactation dairy cows.	Zebeli Q, Terrill SJ, Mazzolari A, Dunn SM, Yang WZ, Ametaj BN	J Dairy Res	10.1017/S0022029911000707	2011	*
Genetics	Genome sequence of the ruminal bacterium Megasphaera elsdenii.	Marx H, Graf AB, Tatto NE, Thallinger GG, Mattanovich D, Sauer M	J Bacteriol	10.1128/JB.05861-11	2011	*
Metabolism	Megasphaera elsdenii JCM1772T normalizes hyperlactate production in the large intestine of fructooligosaccharide-fed rats by stimulating butyrate production.	Hashizume K, Tsukahara T, Yamada K, Koyama H, Ushida K	J Nutr	10.1093/jn/133.10.3187	2003	*

References

#8831

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20460

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#34386 ; Curators of the CIP;
#63098 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 64197

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68367

Automatically annotated from API 20A .

#68380

Automatically annotated from API rID32A .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86129

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID38727.1 )

#119020 Collection of Institut Pasteur ; Curators of the CIP; CIP 106852
* These data were automatically processed and therefore are not curated

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Megasphaera elsdenii DSM 20460 ATCC 25940

Megasphaera elsdenii DSM 20460 ATCC 25940

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