Megasphaera elsdenii (DSM 20460, ATCC 25940, NCIB 8927)

Name: Megasphaera elsdenii (DSM 20460, ATCC 25940, NCIB 8927)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17130

Type strain

Strain Designation LC1

Culture col. no. DSM 20460 ATCC 25940 NCIB 8927 CCUG 64197 JCM 1772 10 more

NCBI tax ID(s) 1064535 907

Special Collections DSMZ CCUG JCM KCTC CIP

Links

Megasphaera elsdenii LC1 is an anaerobe, mesophilic, Gram-negative prokaryote that was isolated from rumen of sheep.

Gram-negative coccus-shaped anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 38727

ATCC 25940,NCIB 8927,CCUG 6199,DSM 20460,JCM 1772,NCIMB 8927,NCDO2263,NCIMB 702264,NCDO2264,NCDO2331,NCIMB 702331,CECT 390,NCFB 2263,NCTC 8927,VTT E-84221,CIP 106852,KCTC 5187,CGMCC 1.2720,LMG 25663,CCUG 64197

@ref 20215

Last LPSN update 2025-12-16 09:51:01

Domain Bacillati

Phylum Bacillota

Class Negativicutes

Order Veillonellales

Family Veillonellaceae

Genus Megasphaera

Species Megasphaera elsdenii

Full scientific name Megasphaera elsdenii (Gutiérrez et al. 1959) Rogosa 1971 (Approved Lists 1980)

Synonyms (1)

"Peptostreptococcus elsdenii"

BacDive ID	Other strains from **Megasphaera elsdenii** (2)	Type strain
158993	M. elsdenii PG-130-P53-9, DSM 100961
158994	M. elsdenii WCA-386-APC-2A, DSM 106891

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
119020	negative	coccus-shaped

Colony morphology

@ref	Incubation period
8831	1-2 days
63098	1 day
119020

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8831	PYG MEDIUM (MODIFIED) (DSMZ Medium 104)	Medium recipe at MediaDive	Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
34386	MEDIUM 20 - for Anaerobic bacteria		Agar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
119020	CIP Medium 20	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8831	positive	growth	37	mesophilic
34386	positive	growth	37	mesophilic
63098	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
8831	anaerobe
63098	anaerobe
119020	anaerobe
125439	obligate anaerobe	98.4

Spore formation

@ref	Spore formation	Confidence
125439		97.8

Murein

@ref	Murein short key	Type
8831	A31	A1gamma m-Dpm-direct

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
68367	17057 ChEBI	cellobiose	-	builds acid from	from API 20A
68367	17634 ChEBI	D-glucose	+	builds acid from	from API 20A
68367	16899 ChEBI	D-mannitol	+	builds acid from	from API 20A
68380	16024 ChEBI	D-mannose	-	fermentation	from API rID32A
68367	16024 ChEBI	D-mannose	-	builds acid from	from API 20A
68367	65327 ChEBI	D-xylose	-	builds acid from	from API 20A
119020	4853 ChEBI	esculin	-	hydrolysis
68367	4853 ChEBI	esculin	-	hydrolysis	from API 20A
68367	5291 ChEBI	gelatin	-	hydrolysis	from API 20A
68367	17754 ChEBI	glycerol	-	builds acid from	from API 20A
68367	30849 ChEBI	L-arabinose	-	builds acid from	from API 20A
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
68367	62345 ChEBI	L-rhamnose	-	builds acid from	from API 20A
68367	17716 ChEBI	lactose	-	builds acid from	from API 20A
68367	17306 ChEBI	maltose	+	builds acid from	from API 20A
68367	6731 ChEBI	melezitose	-	builds acid from	from API 20A
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
119020	17632 ChEBI	nitrate	-	reduction
119020	17632 ChEBI	nitrate	+	respiration
119020	16301 ChEBI	nitrite	-	reduction
68380	16634 ChEBI	raffinose	-	fermentation	from API rID32A
68367	16634 ChEBI	raffinose	-	builds acid from	from API 20A
68367	30911 ChEBI	sorbitol	-	builds acid from	from API 20A
68367	17992 ChEBI	sucrose	-	builds acid from	from API 20A
68367	27082 ChEBI	trehalose	-	builds acid from	from API 20A
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
68367	27897 ChEBI	tryptophan	-	energy source	from API 20A
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A
68367	16199 ChEBI	urea	-	hydrolysis	from API 20A

Antibiotic resistance

@ref	ChEBI	Metabolite	Is antibiotic	Is sensitive	Is resistant
119020	6909	metronidazole

Metabolite production

@ref	Chebi-ID	Metabolite
68380	35581 ChEBI	indole	from API rID32A
119020	35581 ChEBI	indole
68367	35581 ChEBI	indole	from API 20A

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	-	from API rID32A
68367	35581 ChEBI	indole	-	from API 20A

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68380	alanine arylamidase	-	3.4.11.2	from API rID32A
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68380	alkaline phosphatase	-	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68380	alpha-galactosidase	-	3.2.1.22	from API rID32A
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68380	alpha-glucosidase	-	3.2.1.20	from API rID32A
68382	alpha-mannosidase	-	3.2.1.24	from API zym
119020	amylase	-
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
68382	beta-galactosidase	-	3.2.1.23	from API zym
119020	beta-galactosidase	-	3.2.1.23
68380	beta-galactosidase	-	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68382	beta-glucosidase	-	3.2.1.21	from API zym
68380	beta-glucosidase	-	3.2.1.21	from API rID32A
68367	beta-glucosidase	-	3.2.1.21	from API 20A
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
119020	caseinase	-	3.4.21.50
119020	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
119020	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
119020	gelatinase	-
68367	gelatinase	-		from API 20A
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	-		from API rID32A
68380	histidine arylamidase	-		from API rID32A
68380	L-arginine arylamidase	-		from API rID32A
119020	lecithinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
68380	leucine arylamidase	-	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
119020	lipase	-
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
119020	oxidase	-
68380	phenylalanine arylamidase	-		from API rID32A
68380	proline-arylamidase	-	3.4.11.5	from API rID32A
119020	protease	-
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
68382	trypsin	-	3.4.21.4	from API zym
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
119020	tween esterase	-
68380	tyrosine arylamidase	-		from API rID32A
119020	urease	-	3.5.1.5
68380	urease	-	3.5.1.5	from API rID32A
68367	urease	-	3.5.1.5	from API 20A
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	suberin monomers biosynthesis	100	2 of 2
66794	gluconeogenesis	100	8 of 8
66794	sulfopterin metabolism	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	L-lactaldehyde degradation	100	3 of 3
66794	ribulose monophosphate pathway	100	2 of 2
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	aminopropanol phosphate biosynthesis	100	2 of 2
66794	adipate degradation	100	2 of 2
66794	acetoin degradation	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	threonine metabolism	90	9 of 10
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	isoleucine metabolism	87.5	7 of 8
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	palmitate biosynthesis	86.36	19 of 22
66794	propanol degradation	85.71	6 of 7
66794	tetrahydrofolate metabolism	85.71	12 of 14
66794	vitamin B12 metabolism	85.29	29 of 34
66794	vitamin B1 metabolism	84.62	11 of 13
66794	alanine metabolism	82.76	24 of 29
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	peptidoglycan biosynthesis	80	12 of 15
66794	methylglyoxal degradation	80	4 of 5
66794	flavin biosynthesis	80	12 of 15
66794	cellulose degradation	80	4 of 5
66794	phenylacetate degradation (aerobic)	80	4 of 5
66794	photosynthesis	78.57	11 of 14
66794	lipid A biosynthesis	77.78	7 of 9
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	phenylalanine metabolism	76.92	10 of 13
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	glycogen biosynthesis	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	biotin biosynthesis	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	pentose phosphate pathway	72.73	8 of 11
66794	citric acid cycle	71.43	10 of 14
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	pyrimidine metabolism	71.11	32 of 45
66794	starch degradation	70	7 of 10
66794	Entner Doudoroff pathway	70	7 of 10
66794	purine metabolism	69.15	65 of 94
66794	NAD metabolism	66.67	12 of 18
66794	methane metabolism	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	glycolysis	64.71	11 of 17
66794	lipid metabolism	64.52	20 of 31
66794	heme metabolism	64.29	9 of 14
66794	ketogluconate metabolism	62.5	5 of 8
66794	glycine betaine biosynthesis	60	3 of 5
66794	hydrogen production	60	3 of 5
66794	glycogen metabolism	60	3 of 5
66794	propionate fermentation	60	6 of 10
66794	tryptophan metabolism	57.89	22 of 38
66794	d-mannose degradation	55.56	5 of 9
66794	nitrate assimilation	55.56	5 of 9
66794	cysteine metabolism	55.56	10 of 18
66794	histidine metabolism	55.17	16 of 29
66794	oxidative phosphorylation	54.95	50 of 91
66794	proline metabolism	54.55	6 of 11
66794	methionine metabolism	53.85	14 of 26
66794	leucine metabolism	53.85	7 of 13
66794	degradation of sugar acids	52	13 of 25
66794	selenocysteine biosynthesis	50	3 of 6
66794	arginine metabolism	50	12 of 24
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	phenylmercury acetate degradation	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	lysine metabolism	50	21 of 42
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	lactate fermentation	50	2 of 4
66794	sulfate reduction	46.15	6 of 13
66794	isoprenoid biosynthesis	46.15	12 of 26
66794	degradation of hexoses	44.44	8 of 18
66794	tyrosine metabolism	42.86	6 of 14
66794	gallate degradation	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	urea cycle	38.46	5 of 13
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	metabolism of disaccharids	36.36	4 of 11
66794	vitamin B6 metabolism	36.36	4 of 11
66794	degradation of pentoses	35.71	10 of 28
66794	non-pathway related	34.21	13 of 38
66794	pantothenate biosynthesis	33.33	2 of 6
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	octane oxidation	33.33	1 of 3
66794	ascorbate metabolism	31.82	7 of 22
66794	degradation of sugar alcohols	31.25	5 of 16
66794	polyamine pathway	30.43	7 of 23
66794	coenzyme M biosynthesis	30	3 of 10
66794	phenol degradation	30	6 of 20
66794	4-hydroxyphenylacetate degradation	30	3 of 10
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	d-xylose degradation	27.27	3 of 11
66794	cyclohexanol degradation	25	1 of 4
66794	vitamin E metabolism	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	phosphatidylethanolamine bioynthesis	23.08	3 of 13
66794	4-hydroxymandelate degradation	22.22	2 of 9
66794	glutathione metabolism	21.43	3 of 14

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119020	-	-	+	+	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-

API 20A

@ref	IND	URE	GLU	MAN	LAC	SAC	MAL	SAL	XYL	ARA	GEL	ESC	GLY	CEL	MNE	MLZ	RAF	SOR	RHA	TRE	CAT	Spores presentSPOR	GramGRAM	Morphology coccus="+" rod="-"COCC
8831	not determinedn.d.	-	+	+	-	-	+	+/-	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
8831	-	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
8831	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Caprinae (Sheep/Goat)
#Host Body-Site	#Organ	#Rumen

Isolation

@ref	Sample type
8831	rumen of sheep
63098	Rumen of sheep
67770	Rumen of sheep
119020	Animal, Sheep, rumen

Taxonmaps

69479

Global distribution of 16S sequence LC036319 (>99% sequence identity) for Megasphaera elsdenii subclade from Microbeatlas

Aquatic Samples	989
Soil Samples	544
Animal Samples	27646
Plant Samples	254
Total Samples	29433

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Pathogenicity animal	Biosafety level	Biosafety level comment
8831	yes, in single cases	yes, in single cases	1	Risk group (German classification) According to TRBA 466
119020			1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM301049v1 assembly for Megasphaera elsdenii DSM 20460 ATCC 25940	complete	GCA_003010495	1064535.9	2843666722	1064535	98.11
66792	ASM28349v2 assembly for Megasphaera elsdenii DSM 20460	chromosome	GCA_000283495	907.4	2511231071	1064535	86.55

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Megasphaera elsdenii gene for 16S rRNA, partial sequence, strain: DSM 20460, type A	AB609704	1174	1064535
20218	Megasphaera elsdenii gene for 16S rRNA, partial sequence, strain: DSM 20460, type B	AB609705	1174	1064535
20218	Megasphaera elsdenii ATCC 25940 16S ribosomal RNA gene, partial sequence	U95027	1426	1064535
67770	Megasphaera elsdenii gene for 16S ribosomal RNA, partial sequence, strain: JCM 1772	LC036319	1525	907

GC content

8831

GC-content (mol%)52.7

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Megasphaera elsdenii DSM 20460 ATCC 25940
NCBI: GCA_003010495

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	98.40	no
125439	gram_stain	BacteriaNetⓘ	negative	64.00	no
125439	motility	BacteriaNetⓘ	no	71.90	no
125439	spore_formation	BacteriaNetⓘ	no	97.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Megasphaera elsdenii DSM 20460 ATCC 25940
NCBI: GCA_003010495.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	83.71	no
125438	anaerobic	anaerobicⓘ	yes	88.78	yes
125438	aerobic	aerobicⓘ	no	94.61	yes
125438	spore-forming	spore-formingⓘ	no	82.05	no
125438	thermophilic	thermophileⓘ	no	91.71	no
125438	flagellated	motile2+ⓘ	no	84.59	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Transcriptome	Transcriptome and microbiota analysis reveal differences in the cecum of weaning pigs in response to different dietary crude protein levels.	Yu YH, Chen SB, Wang HT, Lin CS, Dybus A, Hukowska-Szematowicz B, Li YH.	Anim Biosci	10.5713/ab.25.0135	2025
	Megasphaera contributes to lactate-driven valerate production in the human gut.	Huertas-Diaz L, Gezer ME, Marietou A, Hosek J, Thams L, Dalgaard LB, Hansen M, Schwab C.	Microbiome	10.1186/s40168-025-02207-6	2025
Genetics	Detection of Colistin Sulfate on Piglet Gastrointestinal Tract Microbiome Alterations.	Fu S, Yuan Y, Tian X, Zhou L, Guo L, Zhang D, He J, Peng C, Qiu Y, Ye C, Liu Y, Zong B.	Vet Sci	10.3390/vetsci9120666	2022
	Megasphaera elsdenii: Its Role in Ruminant Nutrition and Its Potential Industrial Application for Organic Acid Biosynthesis.	Cabral LDS, Weimer PJ.	Microorganisms	10.3390/microorganisms12010219	2024
	Duration of dam contact had a long effect on calf rumen microbiota without affecting growth.	Voland L, Ortiz-Chura A, Tournayre J, Martin B, Bouchon M, Nicolao A, Pomies D, Morgavi DP, Popova M.	Front Vet Sci	10.3389/fvets.2025.1548892	2025
	Structural and digestive characters of a heteropolysaccharide fraction from tea (Camellia sinensis L.) flower.	Chen D, Wang A, Lv J, Tang C, Jin CH, Liu J, Zeng X, Wang L.	Food Chem X	10.1016/j.fochx.2023.101058	2024
	The Pathogenicity of Fusobacterium nucleatum Modulated by Dietary Fibers-A Possible Missing Link between the Dietary Composition and the Risk of Colorectal Cancer.	Nawab S, Bao Q, Ji LH, Luo Q, Fu X, Fan S, Deng Z, Ma W.	Microorganisms	10.3390/microorganisms11082004	2023
	Emerging probiotics: future therapeutics for human gut health.	Duncan SH, Sabater C.	FEMS Microbiol Ecol	10.1093/femsec/fiaf077	2025
	Roles of intralesional bacteria in the initiation and progression of oral squamous cell carcinoma.	Luo Z, Lv S, Lou F, Yan L, Xu J, Kang N, Dong Y, Jin X.	Cancer Med	10.1002/cam4.70209	2024
Genetics	Prophages are associated with extensive CRISPR-Cas auto-immunity.	Nobrega FL, Walinga H, Dutilh BE, Brouns SJJ.	Nucleic Acids Res	10.1093/nar/gkaa1071	2020
	Stable coexistence between an archaeal virus and the dominant methanogen of the human gut.	Baquero DP, Medvedeva S, Martin-Gallausiaux C, Pende N, Sartori-Rupp A, Tachon S, Pedron T, Debarbieux L, Borrel G, Gribaldo S, Krupovic M.	Nat Commun	10.1038/s41467-024-51946-x	2024
	Role of the gut microbiome in three major psychiatric disorders.	Borkent J, Ioannou M, Laman JD, Haarman BCM, Sommer IEC, Sommer IEC.	Psychol Med	10.1017/s0033291722000897	2022
	Influence of dietary carbohydrate profile on the dairy cow rumen meta-proteome.	Mulakala BK, Smith KM, Snider MA, Ayers A, Honan MC, Greenwood SL.	J Dairy Sci	10.3168/jds.2022-21812	2022
Metabolism	Characterization of variations within the rumen metaproteome of Holstein dairy cattle relative to morning feed offering.	Honan MC, Greenwood SL.	Sci Rep	10.1038/s41598-020-59974-5	2020
Metabolism	Substrate Use Prioritization by a Coculture of Five Species of Gut Bacteria Fed Mixtures of Arabinoxylan, Xyloglucan, beta-Glucan, and Pectin.	Liu Y, Heath AL, Galland B, Rehrer N, Drummond L, Wu XY, Bell TJ, Lawley B, Sims IM, Tannock GW.	Appl Environ Microbiol	10.1128/aem.01905-19	2020
Phylogeny	Unique roles of vaginal Megasphaera phylotypes in reproductive health.	Glascock AL, Jimenez NR, Boundy S, Koparde VN, Brooks JP, Edwards DJ, Strauss Iii JF, Jefferson KK, Serrano MG, Buck GA, Fettweis JM.	Microb Genom	10.1099/mgen.0.000526	2021
Metabolism	Can rumen bacteria communicate to each other?	Won MY, Oyama LB, Courtney SJ, Creevey CJ, Huws SA.	Microbiome	10.1186/s40168-020-00796-y	2020
Phylogeny	Microbial Community Analysis of Saliva and Biopsies in Patients With Oral Lichen Planus.	Wang X, Zhao Z, Tang N, Zhao Y, Xu J, Li L, Qian L, Zhang J, Fan Y.	Front Microbiol	10.3389/fmicb.2020.00629	2020
	Potency of cashew nut shell liquid in rumen modulation under different dietary conditions and indication of its surfactant action against rumen bacteria.	Oh S, Suzuki Y, Hayashi S, Suzuki Y, Koike S, Kobayashi Y.	J Anim Sci Technol	10.1186/s40781-017-0150-8	2017
	CMG-biotools, a free workbench for basic comparative microbial genomics.	Vesth T, Lagesen K, Acar O, Ussery D.	PLoS One	10.1371/journal.pone.0060120	2013
Metabolism	Products of gut microbial Toll/interleukin-1 receptor domain NADase activities in gnotobiotic mice and Bangladeshi children with malnutrition.	Weagley JS, Zaydman M, Venkatesh S, Sasaki Y, Damaraju N, Yenkin A, Buchser W, Rodionov DA, Osterman A, Ahmed T, Barratt MJ, DiAntonio A, Milbrandt J, Gordon JI.	Cell Rep	10.1016/j.celrep.2022.110738	2022
	Anti-Correlation between the Dynamics of the Active Site Loop and C-Terminal Tail in Relation to the Homodimer Asymmetry of the Mouse Erythroid 5-Aminolevulinate Synthase.	Na I, Catena D, Kong MJ, Ferreira GC, Uversky VN.	Int J Mol Sci	10.3390/ijms19071899	2018
	Development of a 16S rRNA gene primer and PCR-restriction fragment length polymorphism method for rapid detection of members of the genus Megasphaera and species-level identification.	Ohnishi A, Abe S, Nashirozawa S, Shimada S, Fujimoto N, Suzuki M.	Appl Environ Microbiol	10.1128/aem.00359-11	2011
Phylogeny	Pipeline for amplifying and analyzing amplicons of the V1-V3 region of the 16S rRNA gene.	Allen HK, Bayles DO, Looft T, Trachsel J, Bass BE, Alt DP, Bearson SM, Nicholson T, Casey TA.	BMC Res Notes	10.1186/s13104-016-2172-6	2016
Metabolism	An in vitro model of the horse gut microbiome enables identification of lactate-utilizing bacteria that differentially respond to starch induction.	Biddle AS, Black SJ, Blanchard JL.	PLoS One	10.1371/journal.pone.0077599	2013
Metabolism	Phylogenetic distribution of three pathways for propionate production within the human gut microbiota.	Reichardt N, Duncan SH, Young P, Belenguer A, McWilliam Leitch C, Scott KP, Flint HJ, Louis P.	ISME J	10.1038/ismej.2014.14	2014
	Catabolism of Amino Acids by Megasphaera elsdenii LC1.	Wallace RJ.	Appl Environ Microbiol	10.1128/aem.51.5.1141-1143.1986	1986
	Evaluation of Megasphaera elsdenii supplementation on rumen fermentation, production performance, carcass traits and health of ruminants: a meta-analysis.	Susanto I, Wiryawan KG, Suharti S, Retnani Y, Zahera R, Jayanegara A.	Anim Biosci	10.5713/ab.22.0258	2023
	Megasphaera elsdenii Lactate Degradation Pattern Shifts in Rumen Acidosis Models.	Chen L, Shen Y, Wang C, Ding L, Zhao F, Wang M, Fu J, Wang H.	Front Microbiol	10.3389/fmicb.2019.00162	2019
	Production of medium-chain carboxylic acids by Megasphaera sp. MH with supplemental electron acceptors.	Jeon BS, Choi O, Um Y, Sang BI.	Biotechnol Biofuels	10.1186/s13068-016-0549-3	2016
	The potential of Megasphaera elsdenii isolates to control ruminal acidosis	Henning PH, Horn CH, Steyn DG, Meissner HH, Hagg FM.	Anim Feed Sci Technol	10.1016/j.anifeedsci.2009.12.011	2010
	Dialister pneumosintes associated with human brain abscesses.	Rousee JM, Bermond D, Piemont Y, Tournoud C, Heller R, Kehrli P, Harlay ML, Monteil H, Jaulhac B.	J Clin Microbiol	10.1128/jcm.40.10.3871-3873.2002	2002
Pathogenicity	In vaginal fluid, bacteria associated with bacterial vaginosis can be suppressed with lactic acid but not hydrogen peroxide.	O'Hanlon DE, Moench TR, Cone RA.	BMC Infect Dis	10.1186/1471-2334-11-200	2011
Metabolism	Identification of bacteriocin-like inhibitors from rumen Streptococcus spp. and isolation and characterization of bovicin 255.	Whitford MF, McPherson MA, Forster RJ, Teather RM.	Appl Environ Microbiol	10.1128/aem.67.2.569-574.2001	2001
Enzymology	Isolation of Acidaminococcus fermentans and Megasphaera elsdenii from normal human feces.	Sugihara PT, Sutter VL, Attebery HR, Bricknell KS, Finegold SM.	Appl Microbiol	10.1128/am.27.1.274-275.1974	1974
Metabolism	Effect of pH on growth rates of rumen amylolytic and lactilytic bacteria.	Therion JJ, Kistner A, Kornelius JH.	Appl Environ Microbiol	10.1128/aem.44.2.428-434.1982	1982
Enzymology	Enterohepatic helicobacter in ulcerative colitis: potential pathogenic entities?	Thomson JM, Hansen R, Berry SH, Hope ME, Murray GI, Mukhopadhya I, McLean MH, Shen Z, Fox JG, El-Omar E, Hold GL.	PLoS One	10.1371/journal.pone.0017184	2011
Metabolism	pH and peptide supply can radically alter bacterial populations and short-chain fatty acid ratios within microbial communities from the human colon.	Walker AW, Duncan SH, McWilliam Leitch EC, Child MW, Flint HJ.	Appl Environ Microbiol	10.1128/aem.71.7.3692-3700.2005	2005
Phylogeny	Evaluation of the sodium polyanethol sulfonate disk test for the identification of Peptostreptococcus anaerobius.	Wideman PA, Vargo VL, Citronbaum D, Finegold SM.	J Clin Microbiol	10.1128/jcm.4.4.330-333.1976	1976
Genetics	Assessment of metagenomic workflows using a newly constructed human gut microbiome mock community.	Mori H, Kato T, Ozawa H, Sakamoto M, Murakami T, Taylor TD, Toyoda A, Ohkuma M, Kurokawa K, Ohno H.	DNA Res	10.1093/dnares/dsad010	2023
	Production of R- and S-1,2-propanediol in engineered Lactococcus lactis.	Sato R, Ikeda M, Tanaka T, Ohara H, Aso Y.	AMB Express	10.1186/s13568-021-01276-8	2021
Metabolism	Microbial short-chain fatty acids modulate CD8⁺ T cell responses and improve adoptive immunotherapy for cancer.	Luu M, Riester Z, Baldrich A, Reichardt N, Yuille S, Busetti A, Klein M, Wempe A, Leister H, Raifer H, Picard F, Muhammad K, Ohl K, Romero R, Fischer F, Bauer CA, Huber M, Gress TM, Lauth M, Danhof S, Bopp T, Nerreter T, Mulder IE, Steinhoff U, Hudecek M, Visekruna A.	Nat Commun	10.1038/s41467-021-24331-1	2021
Metabolism	Intestinal integrity and Akkermansia muciniphila, a mucin-degrading member of the intestinal microbiota present in infants, adults, and the elderly.	Collado MC, Derrien M, Isolauri E, de Vos WM, Salminen S.	Appl Environ Microbiol	10.1128/aem.01477-07	2007
	Application of propionate-producing bacterial consortium in ruminal methanogenesis inhibited environment with bromoethanesulfonate as a methanogen direct inhibitor.	Jeong J, Yu C, Kang R, Kim M, Park T.	Front Vet Sci	10.3389/fvets.2024.1422474	2024
	Caenorhabditis Elegans and Probiotics Interactions from a Prolongevity Perspective.	Roselli M, Schifano E, Guantario B, Zinno P, Uccelletti D, Devirgiliis C.	Int J Mol Sci	10.3390/ijms20205020	2019
Metabolism	Chitosan Oligosaccharides Show Protective Effects in Coronary Heart Disease by Improving Antioxidant Capacity via the Increase in Intestinal Probiotics.	Jiang T, Xing X, Zhang L, Liu Z, Zhao J, Liu X.	Oxid Med Cell Longev	10.1155/2019/7658052	2019
Genetics	Complete Genome Sequences of Two Megasphaera elsdenii Strains, NCIMB 702410 and ATCC 25940.	Hatmaker EA, Klingeman DM, O'Dell KB, Riley LA, Papanek B, Guss AM	Microbiol Resour Announc	10.1128/MRA.01430-18	2019
Metabolism	Valerate production by Megasphaera elsdenii isolated from pig feces.	Yoshikawa S, Araoka R, Kajihara Y, Ito T, Miyamoto H, Kodama H	J Biosci Bioeng	10.1016/j.jbiosc.2017.12.016	2018
Phylogeny	Preferential isolation of Megasphaera elsdenii from pig feces.	Kajihara Y, Yoshikawa S, Cho Y, Ito T, Miyamoto H, Kodama H	Anaerobe	10.1016/j.anaerobe.2017.08.013	2017
Metabolism	Comparative genome analysis of Megasphaera sp. reveals niche specialization and its potential role in the human gut.	Shetty SA, Marathe NP, Lanjekar V, Ranade D, Shouche YS	PLoS One	10.1371/journal.pone.0079353	2013
Metabolism	Intraruminal administration of Megasphaera elsdenii modulated rumen fermentation profile in mid-lactation dairy cows.	Zebeli Q, Terrill SJ, Mazzolari A, Dunn SM, Yang WZ, Ametaj BN	J Dairy Res	10.1017/S0022029911000707	2011
Genetics	Genome sequence of the ruminal bacterium Megasphaera elsdenii.	Marx H, Graf AB, Tatto NE, Thallinger GG, Mattanovich D, Sauer M	J Bacteriol	10.1128/JB.05861-11	2011
Metabolism	Megasphaera elsdenii JCM1772T normalizes hyperlactate production in the large intestine of fructooligosaccharide-fed rats by stimulating butyrate production.	Hashizume K, Tsukahara T, Yamada K, Koyama H, Ushida K	J Nutr	10.1093/jn/133.10.3187	2003
Phylogeny	Description of Megasphaera jansseni sp. nov., a novel short- and medium-chain fatty acid-producing species isolated from faeces of a wild boar (Sus scrofa), and a taxonomic note on the family Veillonellaceae.	Heng YC, Lee JKY, Silvaraju S, Kittelmann S.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006937	2025
	Megasphaera lornae sp. nov., Megasphaera hutchinsoni sp. nov., and Megasphaera vaginalis sp. nov.: novel bacteria isolated from the female genital tract.	Srinivasan S, Beamer MA, Fiedler TL, Austin MN, Sizova MV, Strenk SM, Agnew KJ, Gowda GAN, Raftery D, Epstein SS, Fredricks DN, Hillier SL.	Int J Syst Evol Microbiol	10.1099/ijsem.0.004702	2019
Phylogeny	Megasphaera hexanoica sp. nov., a medium-chain carboxylic acid-producing bacterium isolated from a cow rumen.	Jeon BS, Kim S, Sang BI	Int J Syst Evol Microbiol	10.1099/ijsem.0.001888	2017
Phylogeny	Megasphaera indica sp. nov., an obligate anaerobic bacteria isolated from human faeces.	Lanjekar VB, Marathe NP, Ramana VV, Shouche YS, Ranade DR	Int J Syst Evol Microbiol	10.1099/ijs.0.059816-0	2014

References

#8831	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20460
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#34386	; Curators of the CIP;
#63098	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 64197
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68367	Automatically annotated from API 20A .
#68380	Automatically annotated from API rID32A .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119020	Collection of Institut Pasteur ; Curators of the CIP; CIP 106852
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Megasphaera elsdenii (DSM 20460, ATCC 25940, NCIB 8927)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Murein

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Antibiotic resistance

Metabolite production

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API rID32A

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Genome sequences

Genome browser: GCA_003010495

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