Name: Megamonas rupellensis FM1025
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 17127

Type strain:

Species: Megamonas rupellensis

Strain Designation: FM1025

Culture col. no.: DSM 19944, CIP 109788

Strain history: CIP <- 2008, E. Rosenfeld, CNRS, La Rochelle, France: strain FM1025

NCBI tax ID(s): 1122217 (strain), 491921 (species)

SI-ID 360276

CIP 109788, DSM 19944

Special Collections

Section

When using BacDive for research please cite our paper

Megamonas rupellensis FM1025 is an anaerobe, mesophilic, Gram-variable bacterium that was isolated from caecum of a duck.

Gram-variable
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Negativicutes*
	Order	*Selenomonadales*
	Family	*Selenomonadaceae*
	Genus	*Megamonas*
	Species	**Megamonas rupellensis**
	Full Scientific Name (LPSN)	Megamonas rupellensis Chevrot et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #31206]	Gram stain	variable

[Ref.: #31206]	Cell length	1-6 µm

[Ref.: #31206]	Cell shape	rod-shaped
[Ref.: #122316]	Cell shape	rod-shaped

[Ref.: #31206]	Motility	no
[Ref.: #122316]	Motility	no

[Ref.: #31206]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8401]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #8401]

Culture medium growth

[Ref.: #8401]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8401]

Culture medium composition

expand / minimize

[Ref.: #8401]

Culture medium

CHOPPED MEAT MEDIUM (DSMZ Medium 78)

[Ref.: #8401]

Culture medium growth

[Ref.: #8401]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8401]

Culture medium composition

expand / minimize

[Ref.: #37837]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #37837]

Culture medium growth

[Ref.: #37837]

Culture medium composition

expand / minimize

[Ref.: #122316]

Culture medium

CIP Medium 20

[Ref.: #122316]

Culture medium growth

[Ref.: #122316]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8401]	growth	37 °C
[Ref.: #31206]	growth	37 °C
[Ref.: #31206]	optimum	37 °C
[Ref.: #37837]	growth	37 °C

	pH	Kind of pH		pH
[Ref.: #31206]			optimum	7
[Ref.: #31206]			growth	05-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8401]	Oxygen tolerance	anaerobe
[Ref.: #31206]	Oxygen tolerance	anaerobe
[Ref.: #122316]	Oxygen tolerance	anaerobe
[Ref.: #125439]	Oxygen tolerance	obligate aerobe Confidence in %94.8

[Ref.: #31206]

Ability of spore formation

[Ref.: #31206]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31206]	arabinose ChEBI	+	carbon source
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #31206]	cellobiose ChEBI	+	carbon source
[Ref.: #68380]	D-mannose ChEBI	+	fermentation	* from API rID32A
[Ref.: #31206]	glucose ChEBI	+	carbon source
[Ref.: #31206]	glycerol ChEBI	+	carbon source
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #31206]	lactose ChEBI	+	carbon source
[Ref.: #31206]	maltose ChEBI	+	carbon source
[Ref.: #31206]	mannitol ChEBI	+	carbon source
[Ref.: #31206]	mannose ChEBI	+	carbon source
[Ref.: #122316]	nitrate ChEBI	-	reduction
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #122316]	nitrite ChEBI	-	reduction
[Ref.: #31206]	raffinose ChEBI	+	carbon source
[Ref.: #68380]	raffinose ChEBI	+	fermentation	* from API rID32A
[Ref.: #31206]	salicin ChEBI	+	carbon source
[Ref.: #31206]	sorbitol ChEBI	+	carbon source
[Ref.: #31206]	sucrose ChEBI	+	carbon source
[Ref.: #31206]	trehalose ChEBI	+	carbon source
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #122316]		indole ChEBI	no
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API rID32A
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym, API rID32A
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym, API rID32A
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API rID32A
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API rID32A
[Ref.: #122316]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym, API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API rID32A
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #122316]	oxidase	-
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #122316]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #8401]	13660	-	-	+	+	-	+	+	+/-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122316]	12111	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8401]	Sample type/isolated from	caecum of a duck
[Ref.: #8401]	Geographic location (country and/or sea, region)	La Rochelle
[Ref.: #8401]	Country	France
[Ref.: #8401]	Country ISO 3 Code	FRA
[Ref.: #8401]	Continent	Europe

[Ref.: #122316]	Sample type/isolated from	Animal, Duck, intestine
[Ref.: #122316]	Geographic location (country and/or sea, region)	La Rochelle
[Ref.: #122316]	Country	France
[Ref.: #122316]	Country ISO 3 Code	FRA
[Ref.: #122316]	Continent	Europe
[Ref.: #122316]	Isolation date	1998
* marker position based on {}

Isolation sources categories

#Host	#Birds	-
#Host Body-Site	#Gastrointestinal tract	#Large intestine

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU346729 (>99% sequence identity) for Megamonas from Microbeatlas

Aquatic Samples	3117
Soil Samples	863
Animal Samples	48658
Plant Samples	322
Total Samples	52960

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8401]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122316]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8401]	Megamonas rupellensis strain FM1025 16S ribosomal RNA gene, partial sequence	EU346729	1399	GenBank ENA	491921 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Megamonas rupellensis DSM 19944	GCA_000378365	scaffold	GenBank ENA	1122217 tax ID	*
[Ref.: #66792]	Megamonas rupellensis DSM 19944	1122217.3	wgs	PATRIC	1122217 tax ID	*
[Ref.: #66792]	Megamonas rupellensis DSM 19944	2519899689	draft	JGI IMG/M	1122217 tax ID	*

[Ref.: #31206]

GC-content

31-35 mol%

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Megamonas rupellensis DSM 19944 DSM 19944 NCBI: GCA_000378365.1
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	88.269	yes
[Ref.: #125438]	anaerobic	anaerobicⓘ	yes	88.913	yes
[Ref.: #125438]	aerobic	aerobicⓘ	no	93.05	yes
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	80.796	yes
[Ref.: #125438]	thermophile	thermophilicⓘ	no	90.307	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	no	84.409	yes

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Megamonas rupellensis DSM 19944 NCBI: GCA_000378365
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	94.8
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	positive	87.6
[Ref.: #125439]	BacteriaNet	motilityⓘ	no	54.3
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	73.9

Availability in culture collections External links

[Ref.: #8401]

Culture collection no.

DSM 19944, CIP 109788

[Ref.: #86126]

SI-ID 360276

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Megamonas rupellensis sp. nov., an anaerobe isolated from the caecum of a duck.	Chevrot R, Carlotti A, Sopena V, Marchand P, Rosenfeld E	Int J Syst Evol Microbiol	10.1099/ijs.0.2008/001297-0	2008	*

References

#8401

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19944

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31206

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27533 (see below)

#37837 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68380

Automatically annotated from API rID32A .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#86126

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID360276.1 )

#122316 Collection of Institut Pasteur ; Curators of the CIP; CIP 109788

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

#27533

IJSEM 2921 2008 ( DOI 10.1099/ijs.0.2008/001297-0 , PubMed 19060083 )

* These data were automatically processed and therefore are not curated

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Megamonas rupellensis DSM 19944 DSM 19944

Megamonas rupellensis DSM 19944

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