Name: Dialister invisus E7.25
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17122

Type strain:

Species: Dialister invisus

Strain Designation: E7.25

Culture col. no.: CIP 108215, DSM 15470, CCUG 47026, JCM 17566

Strain history: CCUG 47026 <-- J. Downes <-- W. G. Wade strain E7.25.

NCBI tax ID(s): 592028 (strain), 218538 (species)

Section

Dialister invisus E7.25 is an anaerobe, mesophilic bacterium that was isolated from infected root canal, human.

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Negativicutes*
	Order	*Veillonellales*
	Family	*Veillonellaceae*
	Genus	*Dialister*
	Species	**Dialister invisus**
	Full Scientific Name (PNU)	Dialister invisus Downes et al. 2003

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.98

[Ref.: #69480]	Motility	no Confidence in %91.823

[Ref.: #5943]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5943]

Culture medium

CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)

[Ref.: #5943]

Culture medium growth

[Ref.: #5943]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #5943]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #5943]

Culture medium growth

[Ref.: #5943]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #32907]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #32907]

Culture medium growth

[Ref.: #32907]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #5943]	growth	37 °C
[Ref.: #32907]	growth	37 °C
[Ref.: #57462]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #5943]	Temperature range	mesophilic
[Ref.: #32907]	Temperature range	mesophilic
[Ref.: #57462]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5943]	Oxygen tolerance	anaerobe
[Ref.: #57462]	Oxygen tolerance	obligate anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.945

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5943]	Sample type/isolated from	infected root canal, human
[Ref.: #5943]	Geographic location (country and/or sea, region)	London
[Ref.: #5943]	Country	United Kingdom
[Ref.: #5943]	Country ISO 3 Code	GBR
[Ref.: #5943]	Continent	Europe
[Ref.: #5943]	Geographic location	51.5072°/-0.1275°

[Ref.: #57462]	Sample type/isolated from	Human tooth,infected root canal
[Ref.: #57462]	Sampling date	1999-03-30
[Ref.: #57462]	Geographic location (country and/or sea, region)	London
[Ref.: #57462]	Country	United Kingdom
[Ref.: #57462]	Country ISO 3 Code	GBR
[Ref.: #57462]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Human oral cavity in patients with endodontic and periodontal infections
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Host	#Human	-
#Host Body-Site	#Oral cavity and airways	#Tooth

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY162469 (>99% sequence identity) for Dialister invisus subclade from Microbeatlas

Aquatic Samples	9308
Soil Samples	1817
Animal Samples	172260
Plant Samples	640
Total Samples	184025

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5943]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Dialister invisus gene for 16S ribosomal RNA, partial sequence	AB626632	1530	ENA	218538 tax ID
[Ref.: #5943]	Dialister invisus DSM 15470 16S ribosomal RNA gene, partial sequence	AY162469	1524	ENA	592028 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Dialister invisus DSM 15470 DSM 15470	GCA_000160055	scaffold	GenBank	592028 tax ID
[Ref.: #66792]	Dialister invisus DSM 15470	592028.3	wgs	PATRIC	592028 tax ID	*
[Ref.: #66792]	Dialister invisus DSM 15470	645951833	draft	JGI IMG/M	592028 tax ID	*

[Ref.: #5943]	GC-content	45 mol%
[Ref.: #67770]	GC-content	45 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	45.5 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Dialister invisus DSM 15470 DSM 15470 NCBI: GCA_000160055.1
[Ref.: #69480]	anaerobic	yes	100	no
[Ref.: #69480]	gram-positive	no	99.98	yes
[Ref.: #69480]	spore-forming	no	99.945	no
[Ref.: #69480]	thermophile	no	95.379	yes
[Ref.: #69480]	motile	no	91.823	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Dialister invisus DSM 15470 DSM 15470 NCBI: GCA_000160055.1
[Ref.: #69481]	spore-forming	yes	100	no

Availability in culture collections External links

[Ref.: #5943]

Culture collection no.

CIP 108215, DSM 15470, CCUG 47026, JCM 17566

[Ref.: #20218]

Associated Passport(s) in StrainInfo

371257, 371256

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Dialister hominis sp. nov., isolated from human faeces.	Sakamoto M, Ikeyama N, Toyoda A, Murakami T, Mori H, Iino T, Ohkuma M	Int J Syst Evol Microbiol	10.1099/ijsem.0.003797	2020	*
Phylogeny	Dialister invisus sp. nov., isolated from the human oral cavity.	Downes J, Munson M, Wade WG	Int J Syst Evol Microbiol	10.1099/ijs.0.02640-0	2003	*

References

#5943

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15470

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#32907 ; Curators of the CIP;
#57462 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47026

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Dialister invisus DSM 15470 DSM 15470

Dialister invisus DSM 15470 DSM 15470

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