Tsukamurella tyrosinosolvens DSM 44234 is an aerobe, mesophilic, Gram-positive prokaryote that was isolated from clinical material; blood culture of a patient with cardiac pacemaker implants.
Gram-positive rod-shaped aerobe mesophilic genome sequence 16S sequence| @ref 20215 |
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| Domain Bacillati |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Tsukamurellaceae |
| Genus Tsukamurella |
| Species Tsukamurella tyrosinosolvens |
| Full scientific name Tsukamurella tyrosinosolvens Yassin et al. 1997 |
| Synonyms (1) |
| BacDive ID | Other strains from Tsukamurella tyrosinosolvens (8) | Type strain |
|---|---|---|
| 17102 | T. tyrosinosolvens DSM 44316 | |
| 17103 | T. tyrosinosolvens 5297/95, DSM 44330, IMMIB D-1411 | |
| 17104 | T. tyrosinosolvens 185-95, D, DSM 44370 | |
| 17109 | T. tyrosinosolvens Y2, DSM 45557, JCM 15482, KCCM 42885 | |
| 139836 | T. tyrosinosolvens MH-1, DSM 105033 | |
| 152721 | T. tyrosinosolvens CCUG 47473 | |
| 154098 | T. tyrosinosolvens CCUG 52355 | |
| 154960 | T. tyrosinosolvens CCUG 55875 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 11496 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | Medium recipe at MediaDive | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water | ||
| 20028 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 20028 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 20028 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 20028 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 20028 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 20028 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 42109 | MEDIUM 72- for trypto casein soja agar | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |||
| 123704 | CIP Medium 72 | Medium recipe at CIP |
| 67770 | Observationquinones: MK-9, MK-8, MK-10 |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68368 | 27613 ChEBI | amygdalin | - | fermentation | from API 20E |
| 20028 | 22599 ChEBI | arabinose | - | ||
| 68368 | 29016 ChEBI | arginine | + | hydrolysis | from API 20E |
| 20028 | 62968 ChEBI | cellulose | - | ||
| 123704 | 16947 ChEBI | citrate | - | carbon source | |
| 68368 | 16947 ChEBI | citrate | + | assimilation | from API 20E |
| 68379 | 17634 ChEBI | D-glucose | - | fermentation | from API Coryne |
| 68368 | 17634 ChEBI | D-glucose | - | fermentation | from API 20E |
| 68379 | 16899 ChEBI | D-mannitol | - | fermentation | from API Coryne |
| 68368 | 16899 ChEBI | D-mannitol | - | fermentation | from API 20E |
| 68379 | 16988 ChEBI | D-ribose | - | fermentation | from API Coryne |
| 68379 | 65327 ChEBI | D-xylose | - | fermentation | from API Coryne |
| 68379 | 4853 ChEBI | esculin | + | hydrolysis | from API Coryne |
| 123704 | 4853 ChEBI | esculin | + | hydrolysis | |
| 20028 | 28757 ChEBI | fructose | + | ||
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 68368 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20E |
| 20028 | 17234 ChEBI | glucose | + | ||
| 68379 | 28087 ChEBI | glycogen | - | fermentation | from API Coryne |
| 123704 | 606565 ChEBI | hippurate | - | hydrolysis | |
| 68368 | 30849 ChEBI | L-arabinose | - | fermentation | from API 20E |
| 68368 | 62345 ChEBI | L-rhamnose | - | fermentation | from API 20E |
| 68379 | 17716 ChEBI | lactose | - | fermentation | from API Coryne |
| 68368 | 25094 ChEBI | lysine | + | degradation | from API 20E |
| 68379 | 17306 ChEBI | maltose | - | fermentation | from API Coryne |
| 20028 | 29864 ChEBI | mannitol | + | ||
| 68368 | 28053 ChEBI | melibiose | - | fermentation | from API 20E |
| 20028 | 17268 ChEBI | myo-inositol | + | ||
| 68368 | 17268 ChEBI | myo-inositol | - | fermentation | from API 20E |
| 123704 | 17632 ChEBI | nitrate | - | reduction | |
| 123704 | 17632 ChEBI | nitrate | - | respiration | |
| 68379 | 17632 ChEBI | nitrate | - | reduction | from API Coryne |
| 123704 | 16301 ChEBI | nitrite | - | reduction | |
| 68368 | 18257 ChEBI | ornithine | + | degradation | from API 20E |
| 20028 | 16634 ChEBI | raffinose | - | ||
| 20028 | 26546 ChEBI | rhamnose | - | ||
| 68368 | 30911 ChEBI | sorbitol | - | fermentation | from API 20E |
| 20028 | 17992 ChEBI | sucrose | + | ||
| 68379 | 17992 ChEBI | sucrose | - | fermentation | from API Coryne |
| 68368 | 17992 ChEBI | sucrose | - | fermentation | from API 20E |
| 68368 | 27897 ChEBI | tryptophan | - | energy source | from API 20E |
| 68379 | 16199 ChEBI | urea | + | hydrolysis | from API Coryne |
| 68368 | 16199 ChEBI | urea | + | hydrolysis | from API 20E |
| 20028 | 18222 ChEBI | xylose | - |
| @ref | Metabolite | Is sensitive | Is resistant | |
|---|---|---|---|---|
| 123704 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 123704 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68379 | alkaline phosphatase | + | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68379 | alpha-glucosidase | + | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 123704 | amylase | - | ||
| 68368 | arginine dihydrolase | + | 3.5.3.6 | from API 20E |
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 123704 | beta-galactosidase | + | 3.2.1.23 | |
| 68379 | beta-galactosidase | + | 3.2.1.23 | from API Coryne |
| 68368 | beta-galactosidase | - | 3.2.1.23 | from API 20E |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | + | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 123704 | caseinase | - | 3.4.21.50 | |
| 123704 | catalase | + | 1.11.1.6 | |
| 68382 | cystine arylamidase | - | 3.4.11.3 | from API zym |
| 123704 | DNase | - | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 123704 | gamma-glutamyltransferase | + | 2.3.2.2 | |
| 123704 | gelatinase | - | ||
| 68379 | gelatinase | - | from API Coryne | |
| 68368 | gelatinase | - | from API 20E | |
| 123704 | lecithinase | - | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 123704 | lipase | - | ||
| 68382 | lipase (C 14) | + | from API zym | |
| 123704 | lysine decarboxylase | - | 4.1.1.18 | |
| 68368 | lysine decarboxylase | + | 4.1.1.18 | from API 20E |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | from API zym | |
| 123704 | ornithine decarboxylase | - | 4.1.1.17 | |
| 68368 | ornithine decarboxylase | + | 4.1.1.17 | from API 20E |
| 123704 | oxidase | - | ||
| 123704 | phenylalanine ammonia-lyase | - | 4.3.1.24 | |
| 68379 | pyrazinamidase | - | 3.5.1.B15 | from API Coryne |
| 68379 | pyrrolidonyl arylamidase | + | 3.4.19.3 | from API Coryne |
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 123704 | tryptophan deaminase | - | ||
| 68368 | tryptophan deaminase | - | 4.1.99.1 | from API 20E |
| 123704 | tween esterase | + | ||
| 123704 | urease | - | 3.5.1.5 | |
| 68379 | urease | + | 3.5.1.5 | from API Coryne |
| 68368 | urease | + | 3.5.1.5 | from API 20E |
| 68382 | valine arylamidase | - | from API zym |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Infection | #Medical environment | #Clinic | |
| #Infection | #Patient | #Blood culture |
Global distribution of 16S sequence Y12246 (>99% sequence identity) for Tsukamurella from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 66792 | 52700_F01 assembly for Tsukamurella tyrosinosolvens NCTC13231 | complete | 57704 | 90.27 | ||||
| 67770 | IMG-taxon 2639762617 annotated assembly for Tsukamurella tyrosinosolvens DSM 44234 | contig | 57704 | 77.32 | ||||
| 67770 | ASM157515v1 assembly for Tsukamurella tyrosinosolvens CCUG 38499 | contig | 57704 | 63.94 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Tsukamurella tyrosinosolvens strain DSM 44234 16S ribosomal RNA, complete sequence | AY238514 | 1512 | 57704 | ||
| 67770 | Tsukamurella tyrosinosolvens 16S rRNA gene, isolate D-1397 | Y12246 | 1460 | 57704 | ||
| 124043 | Tsukamurella tyrosinosolvens strain DSM 44234 16S ribosomal RNA gene, partial sequence. | PQ248470 | 599 | 57704 | ||
| 124043 | Tsukamurella tyrosinosolvens strain CCUG 38499 16S ribosomal RNA gene, partial sequence. | KX924545 | 1221 | 57704 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | spore_formation | BacteriaNetⓘ | no | 68.10 | no |
| 125439 | motility | BacteriaNetⓘ | no | 77.50 | no |
| 125439 | gram_stain | BacteriaNetⓘ | positive | 99.20 | no |
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 91.20 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 89.19 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 97.56 | yes |
| 125438 | aerobic | aerobicⓘ | yes | 89.61 | yes |
| 125438 | spore-forming | spore-formingⓘ | no | 59.84 | no |
| 125438 | thermophilic | thermophileⓘ | no | 95.58 | yes |
| 125438 | flagellated | motile2+ⓘ | no | 89.75 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Enzymology | Tsukamurella tyrosinosolvens--an unusual case report of bacteremic pneumonia after lung transplantation. | Menard A, Degrange S, Peuchant O, Nguyen TD, Dromer C, Maugein J. | Ann Clin Microbiol Antimicrob | 10.1186/1476-0711-8-30 | 2009 | |
| Enzymology | Characterization of a Tsukamurella pseudo-outbreak by phenotypic tests, 16S rRNA sequencing, pulsed-field gel electrophoresis, and metabolic footprinting. | To KK, Fung AM, Teng JL, Curreem SO, Lee KC, Yuen KY, Lam CW, Lau SK, Woo PC. | J Clin Microbiol | 10.1128/jcm.02845-12 | 2013 | |
| Genetics | Genome-Based Taxonomic Classification of the Phylum Actinobacteria. | Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M. | Front Microbiol | 10.3389/fmicb.2018.02007 | 2018 | |
| Metabolism | Structural elucidation of Tsukamurella pulmonis neutral polysaccharide and its visualization in infected mouse tissues by specific monoclonal antibodies. | Saeed A, Pasciak M, Gorska S, Ceremuga I, Gamian E, Ziolkowski P, Drab M, Gamian A. | Sci Rep | 10.1038/s41598-018-29864-y | 2018 | |
| Phylogeny | Tsukamurella soli sp. nov., isolated from soil. | Weon HY, Yoo SH, Anandham R, Schumann P, Kroppenstedt RM, Kwon SW, Stackebrandt E | Int J Syst Evol Microbiol | 10.1099/ijs.0.014852-0 | 2009 | |
| Phylogeny | Tsukamurella carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete. | Park SW, Kim SM, Park ST, Kim YM | Int J Syst Evol Microbiol | 10.1099/ijs.0.005959-0 | 2009 | |
| Phylogeny | Tsukamurella tyrosinosolvens sp. nov. | Yassin AF, Rainey FA, Burghardt J, Brzezinka H, Schmitt S, Seifert P, Zimmermann O, Mauch H, Gierth D, Lux I, Schaal KP | Int J Syst Bacteriol | 10.1099/00207713-47-3-607 | 1997 |
| #11496 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44234 |
| #20028 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #42109 | ; Curators of the CIP; |
| #54294 | Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 38499 |
| #66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) . |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68368 | Automatically annotated from API 20E . |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #123704 | Collection of Institut Pasteur ; Curators of the CIP; CIP 106771 |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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BacDive in 2025: the core database for prokaryotic strain data