Tsukamurella paurometabola (DSM 20162, ATCC 8368, CECT 3055)

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

• @ref:	66793
  multimedia content:	EM_DSM_20162_1.jpg
  multimedia.multimedia content:	EM_DSM_20162_1.jpg
  caption:	electron microscopic image
  intellectual property rights:	© HZI/Manfred Rohde
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
8586	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)		Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
18633	ISP 2			Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18633	ISP 3			Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18633	ISP 4			Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18633	ISP 5			Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18633	ISP 6			Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18633	ISP 7			Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
40241	MEDIUM 3 - Columbia agar			Columbia agar (39.000 g);distilled water (1000.000 ml)
121640	CIP Medium 3		Medium recipe at CIP

Culture temp

Oxygen tolerance

Halophily

Murein

Observation

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
18633	22599 ChEBI	arabinose	+
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
18633	62968 ChEBI	cellulose	-
121640	16947 ChEBI	citrate	-	carbon source
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
121640	4853 ChEBI	esculin	-	hydrolysis
18633	28757 ChEBI	fructose	+
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
18633	17234 ChEBI	glucose	+
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
121640	606565 ChEBI	hippurate	-	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68368	25094 ChEBI	lysine	+	degradation	from API 20E
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
18633	29864 ChEBI	mannitol	+
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
18633	17268 ChEBI	myo-inositol	+
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
121640	17632 ChEBI	nitrate	-	reduction
121640	17632 ChEBI	nitrate	-	respiration
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
121640	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
18633	16634 ChEBI	raffinose	+
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
18633	26546 ChEBI	rhamnose	+
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
18633	17992 ChEBI	sucrose	+
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid
18633	18222 ChEBI	xylose	+

Antibiotic resistance

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68368	15688 ChEBI	acetoin		from API 20E
68368	16136 ChEBI	hydrogen sulfide		from API 20E
121640	35581 ChEBI	indole
68368	35581 ChEBI	indole		from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test	Indole test
121640	15688 ChEBI	acetoin	-
68368	15688 ChEBI	acetoin	-			from API 20E
121640	17234 ChEBI	glucose		-
68368	35581 ChEBI	indole			-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
121640	alcohol dehydrogenase	-	1.1.1.1
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
121640	amylase	+
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
121640	beta-galactosidase	+	3.2.1.23
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
121640	caseinase	-	3.4.21.50
121640	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
121640	DNase	-
121640	gamma-glutamyltransferase	-	2.3.2.2
121640	gelatinase	+/-
68379	gelatinase	-		from API Coryne
68368	gelatinase	-		from API 20E
121640	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
121640	lipase	-
121640	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
121640	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
121640	oxidase	+
121640	phenylalanine ammonia-lyase	-	4.3.1.24
121640	protease	-
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
121640	tryptophan deaminase	-
68368	tryptophan deaminase	+	4.1.99.1	from API 20E
121640	tween esterase	-
121640	urease	+	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne
68368	urease	+	3.5.1.5	from API 20E

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions	external links
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	octane oxidation	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	threonine metabolism	100	10 of 10
66794	coenzyme A metabolism	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	butanoate fermentation	100	4 of 4
66794	vitamin K metabolism	100	5 of 5
66794	glycolate and glyoxylate degradation	100	6 of 6
66794	gallate degradation	100	5 of 5
66794	cardiolipin biosynthesis	100	7 of 7
66794	adipate degradation	100	2 of 2
66794	aminopropanol phosphate biosynthesis	100	2 of 2
66794	enterobactin biosynthesis	100	3 of 3
66794	biotin biosynthesis	100	4 of 4
66794	L-lactaldehyde degradation	100	3 of 3
66794	valine metabolism	100	9 of 9
66794	acetate fermentation	100	4 of 4
66794	aerobactin biosynthesis	100	1 of 1
66794	palmitate biosynthesis	100	22 of 22
66794	taurine degradation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	methylglyoxal degradation	100	5 of 5
66794	ceramide biosynthesis	100	1 of 1
66794	Entner Doudoroff pathway	90	9 of 10
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	gluconeogenesis	87.5	7 of 8
66794	dTDPLrhamnose biosynthesis	87.5	7 of 8
66794	isoleucine metabolism	87.5	7 of 8
66794	flavin biosynthesis	86.67	13 of 15
66794	alanine metabolism	86.21	25 of 29
66794	photosynthesis	85.71	12 of 14
66794	citric acid cycle	85.71	12 of 14
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	glutathione metabolism	85.71	12 of 14
66794	propanol degradation	85.71	6 of 7
66794	vitamin B12 metabolism	85.29	29 of 34
66794	phenylalanine metabolism	84.62	11 of 13
66794	pyrimidine metabolism	84.44	38 of 45
66794	NAD metabolism	83.33	15 of 18
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	purine metabolism	81.91	77 of 94
66794	pentose phosphate pathway	81.82	9 of 11
66794	glycogen metabolism	80	4 of 5
66794	propionate fermentation	80	8 of 10
66794	factor 420 biosynthesis	80	4 of 5
66794	ethylmalonyl-CoA pathway	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	cellulose degradation	80	4 of 5
66794	phenylacetate degradation (aerobic)	80	4 of 5
66794	heme metabolism	78.57	11 of 14
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	serine metabolism	77.78	7 of 9
66794	4-hydroxymandelate degradation	77.78	7 of 9
66794	d-mannose degradation	77.78	7 of 9
66794	urea cycle	76.92	10 of 13
66794	glycolysis	76.47	13 of 17
66794	lactate fermentation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	proline metabolism	72.73	8 of 11
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	phenol degradation	70	14 of 20
66794	starch degradation	70	7 of 10
66794	myo-inositol biosynthesis	70	7 of 10
66794	leucine metabolism	69.23	9 of 13
66794	vitamin B1 metabolism	69.23	9 of 13
66794	androgen and estrogen metabolism	68.75	11 of 16
66794	non-pathway related	68.42	26 of 38
66794	oxidative phosphorylation	68.13	62 of 91
66794	lipid metabolism	67.74	21 of 31
66794	allantoin degradation	66.67	6 of 9
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	IAA biosynthesis	66.67	2 of 3
66794	methane metabolism	66.67	2 of 3
66794	cysteine metabolism	66.67	12 of 18
66794	arginine metabolism	66.67	16 of 24
66794	isoprenoid biosynthesis	65.38	17 of 26
66794	tryptophan metabolism	63.16	24 of 38
66794	ketogluconate metabolism	62.5	5 of 8
66794	4-hydroxyphenylacetate degradation	60	6 of 10
66794	arachidonate biosynthesis	60	3 of 5
66794	methionine metabolism	57.69	15 of 26
66794	aclacinomycin biosynthesis	57.14	4 of 7
66794	lysine metabolism	57.14	24 of 42
66794	degradation of sugar alcohols	56.25	9 of 16
66794	histidine metabolism	55.17	16 of 29
66794	metabolism of disaccharids	54.55	6 of 11
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	kanosamine biosynthesis II	50	1 of 2
66794	phenylmercury acetate degradation	50	1 of 2
66794	resorcinol degradation	50	1 of 2
66794	suberin monomers biosynthesis	50	1 of 2
66794	tyrosine metabolism	50	7 of 14
66794	glycine metabolism	50	5 of 10
66794	toluene degradation	50	2 of 4
66794	carotenoid biosynthesis	50	11 of 22
66794	ethanol fermentation	50	1 of 2
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	cyclohexanol degradation	50	2 of 4
66794	bile acid biosynthesis, neutral pathway	47.06	8 of 17
66794	sulfate reduction	46.15	6 of 13
66794	nitrate assimilation	44.44	4 of 9
66794	degradation of hexoses	44.44	8 of 18
66794	degradation of sugar acids	44	11 of 25
66794	benzoyl-CoA degradation	42.86	3 of 7
66794	ascorbate metabolism	40.91	9 of 22
66794	lipoate biosynthesis	40	2 of 5
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	3-chlorocatechol degradation	40	2 of 5
66794	D-cycloserine biosynthesis	40	2 of 5
66794	glycine betaine biosynthesis	40	2 of 5
66794	degradation of pentoses	39.29	11 of 28
66794	polyamine pathway	39.13	9 of 23
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	carnitine metabolism	37.5	3 of 8
66794	vitamin B6 metabolism	36.36	4 of 11
66794	d-xylose degradation	36.36	4 of 11
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	lipid A biosynthesis	33.33	3 of 9
66794	pantothenate biosynthesis	33.33	2 of 6
66794	coenzyme M biosynthesis	30	3 of 10
66794	arachidonic acid metabolism	27.78	5 of 18
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	cholesterol biosynthesis	27.27	3 of 11
66794	CMP-KDO biosynthesis	25	1 of 4
66794	phosphatidylethanolamine bioynthesis	23.08	3 of 13

API 20E

API coryne

API zym

API 50CHac

API biotype100

Taxonmaps

69479

Global distribution of 16S sequence X80628 (>99% sequence identity) for Tsukamurella from Microbeatlas

Aquatic Samples	2978
Soil Samples	3431
Animal Samples	5160
Plant Samples	790
Total Samples	12359

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM9222v1 assembly for Tsukamurella paurometabola DSM 20162	complete	GCA_000092225	521096.6	646564587	521096	98.34
67770	51765_C02 assembly for Tsukamurella paurometabola NCTC13232	contig	GCA_900460355	2061.4	2856776985	2061	78.85

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Tsukamurella paurometabola 16S ribosomal RNA gene, complete sequence	AF283280	1510		521096
20218	Tsukamurella paurometabola DSM 20162 16S ribosomal RNA gene, partial sequence	FJ468341	1441		521096
20218	Tsukamurella paurometabolum (Corynebacterium p.) partial 16S rRNA	X53206	1341		521096
20218	Tsukamurella paurometabola 16S rRNA gene, strain DSM20162T	X80628	1488		521096
20218	T.paurometabola gene for 16S ribosomal RNA	Z46751	1474		521096
124043	Tsukamurella paurometabola partial 16S rRNA gene, isolate strain #25 (MBG-DUTH)/DSM 20162 (ELTE)	OU548679	825		2061
124043	Tsukamurella paurometabola DSM 20162 strain ATCC 8368 16S ribosomal RNA gene, partial sequence.	KX924562	1221		521096

GC content

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Strain Streptomyces sp. P-56 Produces Nonactin and Possesses Insecticidal, Acaricidal, Antimicrobial and Plant Growth-Promoting Traits.	Boykova I, Yuzikhin O, Novikova I, Ulianich P, Eliseev I, Shaposhnikov A, Yakimov A, Belimov A.	Microorganisms	10.3390/microorganisms11030764	2023
	Complete genome sequence of Tsukamurella paurometabola type strain (no. 33).	Munk AC, Lapidus A, Lucas S, Nolan M, Tice H, Cheng JF, Del Rio TG, Goodwin L, Pitluck S, Liolios K, Huntemann M, Ivanova N, Mavromatis K, Mikhailova N, Pati A, Chen A, Palaniappan K, Tapia R, Han C, Land M, Hauser L, Chang YJ, Jeffries CD, Brettin T, Yasawong M, Brambilla EM, Rohde M, Sikorski J, Goker M, Detter JC, Woyke T, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP.	Stand Genomic Sci	10.4056/sigs.1894556	2011
Genetics	Cobamide Sharing Is Predicted in the Human Skin Microbiome.	Swaney MH, Sandstrom S, Kalan LR.	mSystems	10.1128/msystems.00677-22	2022
	Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development.	Sandoval-Calderon M, Nguyen DD, Kapono CA, Herron P, Dorrestein PC, Sohlenkamp C.	Front Microbiol	10.3389/fmicb.2015.01465	2015
Metabolism	Gene cluster encoding cholate catabolism in Rhodococcus spp.	Mohn WW, Wilbrink MH, Casabon I, Stewart GR, Liu J, van der Geize R, Eltis LD.	J Bacteriol	10.1128/jb.01169-12	2012
Genetics	Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages.	Klyczek KK, Bonilla JA, Jacobs-Sera D, Adair TL, Afram P, Allen KG, Archambault ML, Aziz RM, Bagnasco FG, Ball SL, Barrett NA, Benjamin RC, Blasi CJ, Borst K, Braun MA, Broomell H, Brown CB, Brynell ZS, Bue AB, Burke SO, Casazza W, Cautela JA, Chen K, Chimalakonda NS, Chudoff D, Connor JA, Cross TS, Curtis KN, Dahlke JA, Deaton BM, Degroote SJ, DeNigris DM, DeRuff KC, Dolan M, Dunbar D, Egan MS, Evans DR, Fahnestock AK, Farooq A, Finn G, Fratus CR, Gaffney BL, Garlena RA, Garrigan KE, Gibbon BC, Goedde MA, Guerrero Bustamante CA, Harrison M, Hartwell MC, Heckman EL, Huang J, Hughes LE, Hyduchak KM, Jacob AE, Kaku M, Karstens AW, Kenna MA, Khetarpal S, King RA, Kobokovich AL, Kolev H, Konde SA, Kriese E, Lamey ME, Lantz CN, Lapin JS, Lawson TO, Lee IY, Lee SM, Lee-Soety JY, Lehmann EM, London SC, Lopez AJ, Lynch KC, Mageeney CM, Martynyuk T, Mathew KJ, Mavrich TN, McDaniel CM, McDonald H, McManus CJ, Medrano JE, Mele FE, Menninger JE, Miller SN, Minick JE, Nabua CT, Napoli CK, Nkangabwa M, Oates EA, Ott CT, Pellerino SK, Pinamont WJ, Pirnie RT, Pizzorno MC, Plautz EJ, Pope WH, Pruett KM, Rickstrew G, Rimple PA, Rinehart CA, Robinson KM, Rose VA, Russell DA, Schick AM, Schlossman J, Schneider VM, Sells CA, Sieker JW, Silva MP, Silvi MM, Simon SE, Staples AK, Steed IL, Stowe EL, Stueven NA, Swartz PT, Sweet EA, Sweetman AT, Tender C, Terry K, Thomas C, Thomas DS, Thompson AR, Vanderveen L, Varma R, Vaught HL, Vo QD, Vonberg ZT, Ware VC, Warrad YM, Wathen KE, Weinstein JL, Wyper JF, Yankauskas JR, Zhang C, Hatfull GF.	PLoS One	10.1371/journal.pone.0180517	2017
Genetics	Protein domain architectures provide a fast, efficient and scalable alternative to sequence-based methods for comparative functional genomics.	Koehorst JJ, Saccenti E, Schaap PJ, Martins Dos Santos VAP, Suarez-Diez M.	F1000Res	10.12688/f1000research.9416.3	2016
Enzymology	Functional characterization of Corynebacterium glutamicum mycothiol S-conjugate amidase.	Si M, Long M, Chaudhry MT, Xu Y, Zhang P, Zhang L, Shen X.	PLoS One	10.1371/journal.pone.0115075	2014
Phylogeny	The plasmid-mediated evolution of the mycobacterial ESX (Type VII) secretion systems.	Newton-Foot M, Warren RM, Sampson SL, van Helden PD, Gey van Pittius NC.	BMC Evol Biol	10.1186/s12862-016-0631-2	2016
Phylogeny	Nucleic acid hybridization studies on Microbacterium, Curtobacterium, Agromyces and related taxa.	Dopfer H, Stackebrandt E, Fiedler F.	J Gen Microbiol	10.1099/00221287-128-8-1697	1982
Phylogeny	Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera.	Gupta RS, Lo B, Son J.	Front Microbiol	10.3389/fmicb.2018.00067	2018
Metabolism	Multiplicity of 3-Ketosteroid-9alpha-Hydroxylase enzymes in Rhodococcus rhodochrous DSM43269 for specific degradation of different classes of steroids.	Petrusma M, Hessels G, Dijkhuizen L, van der Geize R.	J Bacteriol	10.1128/jb.00274-11	2011
Phylogeny	Phylogenetic framework and molecular signatures for the main clades of the phylum Actinobacteria.	Gao B, Gupta RS.	Microbiol Mol Biol Rev	10.1128/mmbr.05011-11	2012
Genetics	Developmental biology of Streptomyces from the perspective of 100 actinobacterial genome sequences.	Chandra G, Chater KF.	FEMS Microbiol Rev	10.1111/1574-6976.12047	2014
	Surfactants tailored by the class Actinobacteria.	Kugler JH, Le Roes-Hill M, Syldatk C, Hausmann R.	Front Microbiol	10.3389/fmicb.2015.00212	2015
Enzymology	Tsukamurella pulmonis bloodstream infection identified by secA1 gene sequencing.	Perez Del Molino Bernal IC, Cano ME, Garcia de la Fuente C, Martinez-Martinez L, Lopez M, Fernandez-Mazarrasa C, Aguero J.	J Clin Microbiol	10.1128/jcm.02545-14	2015
Phylogeny	A PCR-based intergenic spacer region-capillary gel electrophoresis typing method for identification and subtyping of Nocardia species.	Wehrhahn MC, Xiao M, Kong F, Xu YC, Chen SC.	J Clin Microbiol	10.1128/jcm.01311-12	2012
Metabolism	Structural elucidation of Tsukamurella pulmonis neutral polysaccharide and its visualization in infected mouse tissues by specific monoclonal antibodies.	Saeed A, Pasciak M, Gorska S, Ceremuga I, Gamian E, Ziolkowski P, Drab M, Gamian A.	Sci Rep	10.1038/s41598-018-29864-y	2018
	Comparative Study of Eis-like Enzymes from Pathogenic and Nonpathogenic Bacteria.	Green KD, Pricer RE, Stewart MN, Garneau-Tsodikova S.	ACS Infect Dis	10.1021/acsinfecdis.5b00036	2015
Enzymology	Detection of Tropheryma whipplei DNA in clinical specimens by LightCycler real-time PCR.	Sloan LM, Rosenblatt JE, Cockerill FR.	J Clin Microbiol	10.1128/jcm.43.7.3516-3518.2005	2005
Phylogeny	Identification of Mycobacterium species by secA1 sequences.	Zelazny AM, Calhoun LB, Li L, Shea YR, Fischer SH.	J Clin Microbiol	10.1128/jcm.43.3.1051-1058.2005	2005
	The diagnostic targeting of a carbohydrate virulence factor from M.Tuberculosis.	Chan CE, Gotze S, Seah GT, Seeberger PH, Tukvadze N, Wenk MR, Hanson BJ, MacAry PA.	Sci Rep	10.1038/srep10281	2015
Metabolism	Biochemical and structural analysis of aminoglycoside acetyltransferase Eis from Anabaena variabilis.	Pricer RE, Houghton JL, Green KD, Mayhoub AS, Garneau-Tsodikova S.	Mol Biosyst	10.1039/c2mb25341k	2012
	Novel phage display-derived mycolic acid-specific antibodies with potential for tuberculosis diagnosis.	Chan CE, Zhao BZ, Cazenave-Gassiot A, Pang SW, Bendt AK, Wenk MR, MacAry PA, Hanson BJ.	J Lipid Res	10.1194/jlr.d036137	2013
Enzymology	Use of PCR and reverse line blot hybridization macroarray based on 16S-23S rRNA gene internal transcribed spacer sequences for rapid identification of 34 mycobacterium species.	Xiong L, Kong F, Yang Y, Cheng J, Gilbert GL.	J Clin Microbiol	10.1128/jcm.00633-06	2006
Enzymology	The medically important aerobic actinomycetes: epidemiology and microbiology.	McNeil MM, Brown JM.	Clin Microbiol Rev	10.1128/cmr.7.3.357	1994
Metabolism	In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain.	Bustamante D, Segarra S, Tortajada M, Ramon D, Del Cerro C, Auxiliadora Prieto M, Iglesias JR, Rojas A.	Microb Biotechnol	10.1111/1751-7915.13371	2019
Pathogenicity	Pathogenic Nocardia, Rhodococcus, and related organisms are highly susceptible to imidazole antifungals.	Dabbs ER, Naidoo S, Lephoto C, Nikitina N.	Antimicrob Agents Chemother	10.1128/aac.47.4.1476-1478.2003	2003
Enzymology	The actinobacterium Tsukamurella paurometabola has a functionally divergent arylamine N-acetyltransferase (NAT) homolog.	Garefalaki V, Kontomina E, Ioannidis C, Savvidou O, Vagena-Pantoula C, Papavergi MG, Olbasalis I, Patriarcheas D, Fylaktakidou KC, Felfoldi T, Marialigeti K, Fakis G, Boukouvala S	World J Microbiol Biotechnol	10.1007/s11274-019-2755-1	2019
Metabolism	A taxonomically representative strain collection to explore xenobiotic and secondary metabolism in bacteria.	Kontomina E, Garefalaki V, Fylaktakidou KC, Evmorfidou D, Eleftheraki A, Avramidou M, Udoh K, Panopoulou M, Felfoldi T, Marialigeti K, Fakis G, Boukouvala S	PLoS One	10.1371/journal.pone.0271125	2022
Phylogeny	Tsukamurella serpentis sp. nov., isolated from the oral cavity of Chinese cobras (Naja atra).	Tang Y, Teng JLL, Cheung CLW, Ngan AHY, Huang Y, Wong SSY, Yip EKT, Ng KHL, Que TL, Lau SKP, Woo PCY.	Int J Syst Evol Microbiol	10.1099/ijsem.0.001187	2016
Phylogeny	Corynebacterium nuruki sp. nov., isolated from an alcohol fermentation starter.	Shin NR, Jung MJ, Kim MS, Roh SW, Nam YD, Bae JW.	Int J Syst Evol Microbiol	10.1099/ijs.0.027763-0	2011
Phylogeny	Tsukamurella strandjordae sp. nov., a proposed new species causing sepsis.	Kattar MM, Cookson BT, Carlson LD, Stiglich SK, Schwartz MA, Nguyen TT, Daza R, Wallis CK, Yarfitz SL, Coyle MB.	J Clin Microbiol	10.1128/jcm.39.4.1467-1476.2001	2001
Phylogeny	Tsukamurella tyrosinosolvens sp. nov.	Yassin AF, Rainey FA, Burghardt J, Brzezinka H, Schmitt S, Seifert P, Zimmermann O, Mauch H, Gierth D, Lux I, Schaal KP	Int J Syst Bacteriol	10.1099/00207713-47-3-607	1997
Phylogeny	Tsukamurella pulmonis sp. nov.	Yassin AF, Rainey FA, Brzezinka H, Burghardt J, Rifai M, Seifert P, Feldmann K, Schaal KP	Int J Syst Bacteriol	10.1099/00207713-46-2-429	1996