Name: Thiofilum flexile EJ2M-B
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17082

Type strain:

Species: Thiofilum flexile

Strain Designation: EJ2M-B

Culture col. no.: DSM 14609, JCM 11135

Strain history: T. Kohno EJ2M-B.

NCBI tax ID(s): 1123400 (strain), 125627 (species)

SI-ID 101038

DSM 14609, JCM 11135

Section

Thiofilum flexile EJ2M-B is a mesophilic, Gram-negative bacterium that was isolated from bulking activated sludge.

Gram-negative
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Beggiatoales*
	Family	*Leucotrichaceae*
	Genus	*Thiofilum*
	Species	**Thiofilum flexile**
	Full Scientific Name (LPSN)	Thiofilum flexile (Aruga et al. 2002) Boden and Scott 2018

Synonym

Thiothrix flexilis

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.997

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5409]

Culture medium

EGGC (DSMZ Medium 1191)

[Ref.: #5409]

Culture medium growth

[Ref.: #5409]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5409]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #5409]			growth	25 °C
[Ref.: #67770]			growth	25 °C

[Ref.: #5409]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.969

[Ref.: #67770]

Observation

quinones: Q-8

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5409]	Sample type/isolated from	bulking activated sludge
[Ref.: #5409]	Country	Japan
[Ref.: #5409]	Country ISO 3 Code	JPN
[Ref.: #5409]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Bulking activated sludge of pickle manufacturing wastewater treatment plant of pickle manufacturing
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Activated sludge

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB042545 (>99% sequence identity) for Thiotrichales from Microbeatlas

Aquatic Samples	1995
Soil Samples	127
Animal Samples	3155
Plant Samples	46
Total Samples	5323

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5409]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5409]	Thiothrix sp. EJ2M-B gene for 16S ribosomal RNA, partial sequence	AB042545	1417	ENA	125627 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Thiofilum flexile DSM 14609	GCA_000380185	scaffold	GenBank	1123400 tax ID
[Ref.: #66792]	Thiothrix flexilis DSM 14609	1123400.3	wgs	PATRIC	1123400 tax ID	*
[Ref.: #66792]	Thiofilum flexile DSM 14609	2515154111	draft	JGI IMG/M	1123400 tax ID	*

[Ref.: #5409]

GC-content

44 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Thiofilum flexile DSM 14609 DSM 14609 NCBI: GCA_000380185.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thiothrix flexilis DSM 14609 PATRIC: 1123400.3
[Ref.: #69480]	motile	no	71.085	no
[Ref.: #69480]	flagellated	no	80.489	no
[Ref.: #69480]	gram-positive	no	98.835	no
[Ref.: #69480]	anaerobic	no	96.66	no
[Ref.: #69480]	aerobic	yes	79.277	no
[Ref.: #69480]	halophile	no	87.346	no
[Ref.: #69480]	spore-forming	no	93.587	no
[Ref.: #69480]	glucose-util	yes	65.28	no
[Ref.: #69480]	thermophile	no	98.212	yes
[Ref.: #69480]	glucose-ferment	no	85.197	no

Availability in culture collections External links

[Ref.: #5409]

Culture collection no.

DSM 14609, JCM 11135

[Ref.: #86082]

SI-ID 101038

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Characterization of filamentous Eikelboom type 021N bacteria and description of Thiothrix disciformis sp. nov. and Thiothrix flexilis sp. nov.	Aruga S, Kamagata Y, Kohno T, Hanada S, Nakamura K, Kanagawa T	Int J Syst Evol Microbiol	10.1099/00207713-52-4-1309	2002	*

References

#5409

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14609

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86082

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID101038.1 )

* These data were automatically processed and therefore are not curated

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Thiofilum flexile DSM 14609 DSM 14609

Thiothrix flexilis DSM 14609

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