Name: Pseudothermotoga lettingae TMO
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17070

Type strain:

Species: Pseudothermotoga lettingae

Strain Designation: TMO

Culture col. no.: DSM 14385, ATCC BAA-301, NBRC 107922

Strain history: <- M. Balk, Wageningen University, Laboratory of Microbiology, Netherlands; TMO <- M. Balk {2000}

NCBI tax ID(s): 177758 (species)

SI-ID 101014

ATCC BAA-301, DSM 14385

Section

Pseudothermotoga lettingae TMO is an anaerobe, thermophilic bacterium that was isolated from thermophilic sulfate-reducing bioreactor.

anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Thermotogota*
	Class	*Thermotogae*
	Order	*Thermotogales*
	Family	*Thermotogaceae*
	Genus	*Pseudothermotoga*
	Species	**Pseudothermotoga lettingae**
	Full Scientific Name (LPSN)	Pseudothermotoga lettingae (Balk et al. 2002) Bhandari and Gupta 2014

Synonym

Thermotoga lettingae

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5332]

Culture medium

PSEUDOTHERMOTOGA LETTINGAE MEDIUM (DSMZ Medium 664a)

[Ref.: #5332]

Culture medium growth

[Ref.: #5332]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5332]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #5332]			growth	65 °C

[Ref.: #5332]

Temperature range

thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5332]

Oxygen tolerance

anaerobe

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5332]	Sample type/isolated from	thermophilic sulfate-reducing bioreactor
[Ref.: #5332]	Geographic location (country and/or sea, region)	Wageningen
[Ref.: #5332]	Country	Netherlands
[Ref.: #5332]	Country ISO 3 Code	NLD
[Ref.: #5332]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Bioreactor	-
#Condition	#Sulfuric	-
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF355615 (>99% sequence identity) for Pseudothermotoga from Microbeatlas

Aquatic Samples	31
Soil Samples	22
Animal Samples	3
Plant Samples
Total Samples	56

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5332]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5332]	Thermotoga sp. TMO 16S ribosomal RNA gene, complete sequence	AF355615	1513	ENA	416591 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudothermotoga lettingae TMO	GCA_000017865	complete	GenBank	416591 tax ID	*
[Ref.: #66792]	Pseudothermotoga lettingae TMO	641228511	complete	JGI IMG/M	416591 tax ID	*

[Ref.: #5332]

GC-content

39.2 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudothermotoga lettingae TMO TMO NCBI: GCA_000017865.1
[Ref.: #69480]	motile	yes	80.957	no
[Ref.: #69480]	flagellated	no	92.835	no
[Ref.: #69480]	gram-positive	no	95.937	no
[Ref.: #69480]	anaerobic	yes	99.655	yes
[Ref.: #69480]	aerobic	no	97.987	yes
[Ref.: #69480]	halophile	no	91.281	no
[Ref.: #69480]	spore-forming	no	87.437	no
[Ref.: #69480]	thermophile	yes	100	yes
[Ref.: #69480]	glucose-util	yes	90.311	no
[Ref.: #69480]	glucose-ferment	no	56.706	no

Availability in culture collections External links

[Ref.: #5332]

Culture collection no.

DSM 14385, ATCC BAA-301, NBRC 107922

[Ref.: #86070]

SI-ID 101014

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Thermotoga lettingae sp. nov., a novel thermophilic, methanol-degrading bacterium isolated from a thermophilic anaerobic reactor.	Balk M, Weijma J, Stams AJM	Int J Syst Evol Microbiol	10.1099/00207713-52-4-1361	2002	*

References

#5332

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14385

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#86070

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID101014.1 )

* These data were automatically processed and therefore are not curated

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Pseudothermotoga lettingae TMO TMO

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