Name: Thermotoga neapolitana NS-E
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17061

Type strain:

Species: Thermotoga neapolitana

Strain Designation: NS-E

Culture col. no.: DSM 4359, ATCC 49049, NBRC 107923

Strain history: <- K.O. Stetter <- H.W. Jannasch, NS-E

NCBI tax ID(s): 309803 (strain), 2337 (species)

SI-ID 42266

ATCC 49049, DSM 4359

Other strains from Thermotoga neapolitana

T. neapolitana LA4, DSM 5068

Section

Thermotoga neapolitana NS-E is an anaerobe, hyperthermophilic, Gram-negative bacterium that was isolated from submarine hot spring.

Gram-negative
anaerobe
hyperthermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Thermotogota*
	Class	*Thermotogae*
	Order	*Thermotogales*
	Family	*Thermotogaceae*
	Genus	*Thermotoga*
	Species	**Thermotoga neapolitana**
	Full Scientific Name (LPSN)	Thermotoga neapolitana Jannasch et al. 1989

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.811

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1545]

Culture medium

THERMOTOGA TF(C) MEDIUM (DSMZ Medium 613)

[Ref.: #1545]

Culture medium growth

[Ref.: #1545]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1545]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #1545]			growth	85 °C

[Ref.: #1545]	Temperature range	hyperthermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %100

Information on physiology and metabolism Physiology and metabolism

[Ref.: #1545]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.715

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1545]	Sample type/isolated from	submarine hot spring
[Ref.: #1545]	Geographic location (country and/or sea, region)	Bay of Naples
[Ref.: #1545]	Country	Italy
[Ref.: #1545]	Country ISO 3 Code	ITA
[Ref.: #1545]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB039768 (>99% sequence identity) for Thermotoga from Microbeatlas

Aquatic Samples	246
Soil Samples	93
Animal Samples	428
Plant Samples	10
Total Samples	777

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1545]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Thermotoga neapolitana gene for 16S rRNA	AB039768	1482	ENA	309803 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thermotoga neapolitana DSM 4359	GCA_000018945	complete	GenBank	309803 tax ID	*
[Ref.: #66792]	Thermotoga neapolitana DSM 4359	309803.4	complete	PATRIC	309803 tax ID	*
[Ref.: #66792]	Thermotoga neapolitana DSM 4359	643348584	complete	JGI IMG/M	309803 tax ID	*

[Ref.: #1545]

GC-content

46 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Thermotoga neapolitana DSM 4359 DSM 4359 NCBI: GCA_000018945.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thermotoga neapolitana DSM 4359 DSM 4359 NCBI: GCA_000018945.1
[Ref.: #69480]	motile	yes	85.885	no
[Ref.: #69480]	flagellated	no	89.114	no
[Ref.: #69480]	gram-positive	no	93.689	no
[Ref.: #69480]	anaerobic	yes	99.481	yes
[Ref.: #69480]	aerobic	no	98.207	yes
[Ref.: #69480]	halophile	no	92.408	no
[Ref.: #69480]	spore-forming	no	91.277	no
[Ref.: #69480]	thermophile	yes	100	yes
[Ref.: #69480]	glucose-util	yes	88.949	no
[Ref.: #69480]	glucose-ferment	yes	54.185	no

Availability in culture collections External links

[Ref.: #1545]

Culture collection no.

DSM 4359, ATCC 49049, NBRC 107923

[Ref.: #86061]

SI-ID 42266

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Introducing capnophilic lactic fermentation in a combined dark-photo fermentation process: a route to unparalleled H2 yields.	Dipasquale L, Adessi A, d'Ippolito G, Rossi F, Fontana A, De Philippis R	Appl Microbiol Biotechnol	10.1007/s00253-014-6231-4	2014	*
Enzymology	Cloning and biochemical characterization of a glucosidase from a marine bacterium Aeromonas sp. HC11e-3.	Huang X, Zhao Y, Dai Y, Wu G, Shao Z, Zeng Q, Liu Z	World J Microbiol Biotechnol	10.1007/s11274-012-1145-8	2012	*
Metabolism	Enzymatic transformation of ginsenosides Re, Rg1, and Rf to ginsenosides Rg2 and aglycon PPT by using beta-glucosidase from Thermotoga neapolitana.	Bi YF, Wang XZ, Jiang S, Liu JS, Zheng MZ, Chen P	Biotechnol Lett	10.1007/s10529-019-02665-7	2019	*
Enzymology	Biochemical characterization of a thermostable beta-1,3-xylanase from the hyperthermophilic eubacterium, Thermotoga neapolitana strain DSM 4359.	Okazaki F, Nakashima N, Ogino C, Tamaru Y, Kondo A	Appl Microbiol Biotechnol	10.1007/s00253-012-4555-5	2012	*
Enzymology	Expression, crystallization and preliminary X-ray diffraction studies of thermostable beta-1,3-xylanase from Thermotoga neapolitana strain DSM 4359.	Okazaki F, Ogino C, Kondo A, Mikami B, Kurebayashi Y, Tsuruta H	Acta Crystallogr Sect F Struct Biol Cryst Commun	10.1107/S1744309111017222	2011	*
Enzymology	Purification and biochemical characterization of a native invertase from the hydrogen-producing Thermotoga neapolitana (DSM 4359).	Dipasquale L, Gambacorta A, Siciliano RA, Mazzeo MF, Lama L	Extremophiles	10.1007/s00792-008-0222-2	2009	*
Genetics	Chromosome evolution in the Thermotogales: large-scale inversions and strain diversification of CRISPR sequences.	DeBoy RT, Mongodin EF, Emerson JB, Nelson KE	J Bacteriol	10.1128/JB.188.7.2364-2374.2006	2006	*
Phylogeny	Growth requirements of hyperthermophilic sulfur-dependent heterotrophic archaea isolated from a shallow submarine geothermal system with reference to their essential amino acids.	Hoaki T, Nishijima M, Kato M, Adachi K, Mizobuchi S, Hanzawa N, Maruyama T	Appl Environ Microbiol	10.1128/aem.60.8.2898-2904.1994	1994	*

References

#1545

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4359

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86061

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID42266.1 )

* These data were automatically processed and therefore are not curated

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Thermotoga neapolitana DSM 4359 DSM 4359

Thermotoga neapolitana DSM 4359 DSM 4359

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