Name: Thermotoga maritima MSB8
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 17060

Type strain:

Species: Thermotoga maritima

Strain Designation: MSB8

Culture col. no.: DSM 3109, JCM 10099, ATCC 43589, NBRC 100826

Strain history: DSM 3109 <-- K. O. Stetter MSB8.

NCBI tax ID(s): 243274 (strain), 2336 (species)

SI-ID 41926

ATCC 43589, DSM 3109, JCM 10099, NBRC 100826

Section

Thermotoga maritima MSB8 is an anaerobe, Gram-negative bacterium that was isolated from anaerobic marine mud.

Gram-negative
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Thermotogota*
	Class	*Thermotogae*
	Order	*Thermotogales*
	Family	*Thermotogaceae*
	Genus	*Thermotoga*
	Species	**Thermotoga maritima**
	Full Scientific Name (LPSN)	Thermotoga maritima Stetter and Huber 1986

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %93.435

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1306]

Culture medium

THERMOTOGA TF(D) MEDIUM (DSMZ Medium 1232)

[Ref.: #1306]

Culture medium growth

[Ref.: #1306]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1306]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #1306]			growth	80 °C
[Ref.: #67770]			growth	80 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #1306]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.194

[Ref.: #69480]	Ability of spore formation	no Confidence in %90.976
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	sulfopterin metabolism	100	4 of 4
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	palmitate biosynthesis	95.45	21 of 22
[Ref.: #66794]	pentose phosphate pathway	90.91	10 of 11
[Ref.: #66794]	starch degradation	90	9 of 10
[Ref.: #66794]	threonine metabolism	90	9 of 10
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	peptidoglycan biosynthesis	86.67	13 of 15
[Ref.: #66794]	NAD metabolism	83.33	15 of 18
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	valine metabolism	77.78	7 of 9
[Ref.: #66794]	d-mannose degradation	77.78	7 of 9
[Ref.: #66794]	vitamin B1 metabolism	76.92	10 of 13
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	isoleucine metabolism	75	6 of 8
[Ref.: #66794]	C4 and CAM-carbon fixation	75	6 of 8
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	methionine metabolism	73.08	19 of 26
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	purine metabolism	70.21	66 of 94
[Ref.: #66794]	Entner Doudoroff pathway	70	7 of 10
[Ref.: #66794]	pyrimidine metabolism	68.89	31 of 45
[Ref.: #66794]	degradation of sugar alcohols	68.75	11 of 16
[Ref.: #66794]	glutamate and glutamine metabolism	67.86	19 of 28
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	66.67	6 of 9
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	enterobactin biosynthesis	66.67	2 of 3
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	glycolate and glyoxylate degradation	66.67	4 of 6
[Ref.: #66794]	isoprenoid biosynthesis	65.38	17 of 26
[Ref.: #66794]	tetrahydrofolate metabolism	64.29	9 of 14
[Ref.: #66794]	d-xylose degradation	63.64	7 of 11
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	gluconeogenesis	62.5	5 of 8
[Ref.: #66794]	ketogluconate metabolism	62.5	5 of 8
[Ref.: #66794]	phenylalanine metabolism	61.54	8 of 13
[Ref.: #66794]	degradation of hexoses	61.11	11 of 18
[Ref.: #66794]	degradation of pentoses	60.71	17 of 28
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	glycogen metabolism	60	3 of 5
[Ref.: #66794]	hydrogen production	60	3 of 5
[Ref.: #66794]	histidine metabolism	58.62	17 of 29
[Ref.: #66794]	ubiquinone biosynthesis	57.14	4 of 7
[Ref.: #66794]	citric acid cycle	57.14	8 of 14
[Ref.: #66794]	oxidative phosphorylation	57.14	52 of 91
[Ref.: #66794]	cysteine metabolism	55.56	10 of 18
[Ref.: #66794]	urea cycle	53.85	7 of 13
[Ref.: #66794]	tryptophan metabolism	52.63	20 of 38
[Ref.: #66794]	alanine metabolism	51.72	15 of 29
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	CMP-KDO biosynthesis	50	2 of 4
[Ref.: #66794]	lactate fermentation	50	2 of 4
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	acetate fermentation	50	2 of 4
[Ref.: #66794]	suberin monomers biosynthesis	50	1 of 2
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	arginine metabolism	50	12 of 24
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	adipate degradation	50	1 of 2
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	lysine metabolism	50	21 of 42
[Ref.: #66794]	propionate fermentation	50	5 of 10
[Ref.: #66794]	non-pathway related	47.37	18 of 38
[Ref.: #66794]	leucine metabolism	46.15	6 of 13
[Ref.: #66794]	metabolism of disaccharids	45.45	5 of 11
[Ref.: #66794]	CO2 fixation in Crenarchaeota	44.44	4 of 9
[Ref.: #66794]	degradation of sugar acids	44	11 of 25
[Ref.: #66794]	reductive acetyl coenzyme A pathway	42.86	3 of 7
[Ref.: #66794]	propanol degradation	42.86	3 of 7
[Ref.: #66794]	cardiolipin biosynthesis	42.86	3 of 7
[Ref.: #66794]	tyrosine metabolism	42.86	6 of 14
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
[Ref.: #66794]	creatinine degradation	40	2 of 5
[Ref.: #66794]	coenzyme M biosynthesis	40	4 of 10
[Ref.: #66794]	myo-inositol biosynthesis	40	4 of 10
[Ref.: #66794]	chlorophyll metabolism	38.89	7 of 18
[Ref.: #66794]	lipid metabolism	38.71	12 of 31
[Ref.: #66794]	dTDPLrhamnose biosynthesis	37.5	3 of 8
[Ref.: #66794]	proline metabolism	36.36	4 of 11
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	ascorbate metabolism	31.82	7 of 22
[Ref.: #66794]	phenylpropanoid biosynthesis	30.77	4 of 13
[Ref.: #66794]	sulfate reduction	30.77	4 of 13
[Ref.: #66794]	vitamin B6 metabolism	27.27	3 of 11
[Ref.: #66794]	polyamine pathway	26.09	6 of 23
[Ref.: #66794]	vitamin E metabolism	25	1 of 4
[Ref.: #66794]	biotin biosynthesis	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	23.08	3 of 13
[Ref.: #66794]	molybdenum cofactor biosynthesis	22.22	2 of 9
[Ref.: #66794]	glutathione metabolism	21.43	3 of 14
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1306]	Sample type/isolated from	anaerobic marine mud
[Ref.: #1306]	Geographic location (country and/or sea, region)	Vulcano island
[Ref.: #1306]	Country	Italy
[Ref.: #1306]	Country ISO 3 Code	ITA
[Ref.: #1306]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Geothermally heated sea sediment
[Ref.: #67770]	Geographic location (country and/or sea, region)	Porto di Levante, Vulcano
[Ref.: #67770]	Country	Italy
[Ref.: #67770]	Country ISO 3 Code	ITA
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Terrestrial	#Mud (Sludge)
#Condition	#Anoxic (anaerobic)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence M21774 (>99% sequence identity) for Thermotoga from Microbeatlas

Aquatic Samples	246
Soil Samples	93
Animal Samples	428
Plant Samples	10
Total Samples	777

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1306]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #1306]	T.maritima ribosomal RNA small subunit	M21774	1562	GenBank ENA	2336 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thermotoga maritima MSB8	2554235387	complete	JGI IMG/M	243274 tax ID	*
[Ref.: #66792]	Thermotoga maritima MSB8	GCA_000390265	complete	GenBank ENA	243274 tax ID	*
[Ref.: #66792]	Thermotoga maritima MSB8	GCA_000978555	complete	GenBank ENA	243274 tax ID	*
[Ref.: #66792]	Thermotoga maritima MSB8	GCA_000008545	complete	GenBank ENA	243274 tax ID	*
[Ref.: #66792]	Thermotoga maritima MSB8	243274.5	complete	PATRIC	243274 tax ID	*
[Ref.: #66792]	Thermotoga maritima MSB8	243274.17	complete	PATRIC	243274 tax ID	*

[Ref.: #1306]	GC-content	46.0 mol%
[Ref.: #67770]	GC-content	46 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Thermotoga maritima MSB8 PATRIC: 243274.18
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thermotoga maritima MSB8 MSB8 NCBI: GCA_000230655.3
[Ref.: #69480]	motile	yes	85.63	no
[Ref.: #69480]	flagellated	no	88.598	no
[Ref.: #69480]	gram-positive	no	93.435	no
[Ref.: #69480]	anaerobic	yes	99.194	yes
[Ref.: #69480]	aerobic	no	97.984	yes
[Ref.: #69480]	halophile	no	89.934	no
[Ref.: #69480]	spore-forming	no	90.976	no
[Ref.: #69480]	glucose-util	yes	87.457	no
[Ref.: #69480]	thermophile	yes	100	yes
[Ref.: #69480]	glucose-ferment	yes	55.866	no

Availability in culture collections External links

[Ref.: #1306]

Culture collection no.

DSM 3109, JCM 10099, ATCC 43589, NBRC 100826

[Ref.: #86060]

SI-ID 41926

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	D-Amino acid dipeptide production utilizing D-alanine-D-alanine ligases with novel substrate specificity.	Sato M, Kirimura K, Kino K	J Biosci Bioeng	10.1263/jbb.99.623	2005	*
Metabolism	Ligands of thermophilic ABC transporters encoded in a newly sequenced genomic region of Thermotoga maritima MSB8 screened by differential scanning fluorimetry.	Boucher N, Noll KM	Appl Environ Microbiol	10.1128/AEM.05418-11	2011	*
Enzymology	Structure-based modification of D-alanine-D-alanine ligase from Thermotoga maritima ATCC 43589 for depsipeptide synthesis.	Nakagawa T, Satake R, Sato M, Kino K	Biosci Biotechnol Biochem	10.1271/bbb.100832	2011	*
Enzymology	Characterization of a recombinant thermostable L: -rhamnose isomerase from Thermotoga maritima ATCC 43589 and its application in the production of L-lyxose and L-mannose.	Park CS, Yeom SJ, Lim YR, Kim YS, Oh DK	Biotechnol Lett	10.1007/s10529-010-0385-7	2010	*
Metabolism	Enhancement of the alcoholytic activity of alpha-amylase AmyA from Thermotoga maritima MSB8 (DSM 3109) by site-directed mutagenesis.	Damian-Almazo JY, Moreno A, Lopez-Munguia A, Soberon X, Gonzalez-Munoz F, Saab-Rincon G	Appl Environ Microbiol	10.1128/AEM.00121-08	2008	*
Enzymology	Thermostable ATP regeneration system using polyphosphate kinase from Thermosynechococcus elongatus BP-1 for D-amino acid dipeptide synthesis.	Sato M, Masuda Y, Kirimura K, Kino K	J Biosci Bioeng	10.1263/jbb.103.179	2007	*
Enzymology	Substrate specificity of thermostable D-alanine-D-alanine ligase from Thermotoga maritima ATCC 43589.	Sato M, Kirimura K, Kino K	Biosci Biotechnol Biochem	10.1271/bbb.60307	2006	*
Enzymology	[Expression and purification of thermostable alpha-glucuronidase from Thermotoga maritima].	Xue YM, Mao ZG, Shao WL	Sheng Wu Gong Cheng Xue Bao		2004	*
Metabolism	Analysis of a Thermotoga maritima DNA fragment encoding two similar thermostable cellulases, CelA and CelB, and characterization of the recombinant enzymes.	Liebl W, Ruile P, Bronnenmeier K, Riedel K, Lottspeich F, Greif I	Microbiology (Reading)	10.1099/00221287-142-9-2533	1996	*
	Characterisation of a hyperthermophilic transketolase from Thermotoga maritima DSM3109 as a biocatalyst for 7-keto-octuronic acid synthesis.	Cardenas-Fernandez M, Subrizi F, Dobrijevic D, Hailes HC, Ward JM	Org Biomol Chem	10.1039/d1ob01237a	2021	*

References

#1306

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3109

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86060

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID41926.1 )

* These data were automatically processed and therefore are not curated

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Thermotoga maritima MSB8

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