Name: Petrotoga halophila MET-B
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17051

Type strain:

Species: Petrotoga halophila

Strain Designation: MET-B

Culture col. no.: DSM 16923, CCUG 50214

Strain history: <- M.-L. Fardeau, IRD, Marseille; MET-B <- E. Miranda-Tello {2003}

NCBI tax ID(s): 1122953 (strain), 301141 (species)

SI-ID 297272

CCUG 50214, DSM 16923

Section

Petrotoga halophila MET-B is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from oil well TBM.

Gram-negative
rod-shaped
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Thermotogota*
	Class	*Thermotogae*
	Order	*Petrotogales*
	Family	*Petrotogaceae*
	Genus	*Petrotoga*
	Species	**Petrotoga halophila**
	Full Scientific Name (LPSN)	Petrotoga halophila Miranda-Tello et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #31847]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.932

[Ref.: #31847]	Cell length	23.5 µm

[Ref.: #31847]	Cell width	0.6 µm

[Ref.: #31847]	Cell shape	rod-shaped

[Ref.: #31847]	Motility	no

[Ref.: #58792]

Incubation period

4 days

[Ref.: #31847]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6645]

Culture medium

PETROTOGA HALOPHILA MEDIUM (DSMZ Medium 718a)

[Ref.: #6645]

Culture medium growth

[Ref.: #6645]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6645]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #6645]	growth	60 °C
[Ref.: #31847]	growth	35-65 °C
[Ref.: #31847]	optimum	55 °C
[Ref.: #58792]	growth	56 °C

[Ref.: #6645]	Temperature range	thermophilic
[Ref.: #31847]	Temperature range	thermophilic
[Ref.: #58792]	Temperature range	thermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %99.999

	pH	Kind of pH		pH
[Ref.: #31847]			optimum	6.5
[Ref.: #31847]			growth	5.5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #6645]	Oxygen tolerance	anaerobe
[Ref.: #31847]	Oxygen tolerance	anaerobe
[Ref.: #58792]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #31847]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.954

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31847]		NaCl		growth	0.5-10	%
[Ref.: #31847]		NaCl		optimum	2	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31847]	arabinose ChEBI	+	carbon source
[Ref.: #31847]	cellobiose ChEBI	+	carbon source
[Ref.: #31847]	fructose ChEBI	+	carbon source
[Ref.: #31847]	galactose ChEBI	+	carbon source
[Ref.: #31847]	glucose ChEBI	+	carbon source
[Ref.: #31847]	lactose ChEBI	+	carbon source
[Ref.: #31847]	maltose ChEBI	+	carbon source
[Ref.: #31847]	pyruvate ChEBI	+	carbon source
[Ref.: #31847]	rhamnose ChEBI	+	carbon source
[Ref.: #31847]	ribose ChEBI	+	carbon source
[Ref.: #31847]	sucrose ChEBI	+	carbon source
[Ref.: #31847]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6645]	Sample type/isolated from	oil well TBM
[Ref.: #6645]	Geographic location (country and/or sea, region)	Tchibouella oilfield
[Ref.: #6645]	Country	Republic of the Congo
[Ref.: #6645]	Country ISO 3 Code	COG
[Ref.: #6645]	Continent	Africa

[Ref.: #58792]	Sample type/isolated from	Oil well TBM
[Ref.: #58792]	Sampling date	1993
* marker position based on {}

Isolation sources categories

#Engineered

#Industrial

#Oil reservoir

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY800102 (>99% sequence identity) for Petrotoga from Microbeatlas

Aquatic Samples	87
Soil Samples	7
Animal Samples	10
Plant Samples
Total Samples	104

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6645]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6645]	Petrotoga halophila strain MET-B 16S ribosomal RNA gene, partial sequence	AY800102	1477	ENA	301141 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Petrotoga halophila DSM 16923	GCA_002924615	scaffold	GenBank	1122953 tax ID	*
[Ref.: #66792]	Petrotoga halophila DSM 16923	1122953.3	wgs	PATRIC	1122953 tax ID	*
[Ref.: #66792]	Petrotoga halophila DSM 16923	2556793017	draft	JGI IMG/M	1122953 tax ID	*

[Ref.: #31847]

GC-content

34.6 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Petrotoga halophila DSM 16923 DSM 16923 NCBI: GCA_002924615.1
[Ref.: #69481]	spore-forming	no	74	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Petrotoga halophila DSM 16923 DSM 16923 NCBI: GCA_002924615.1
[Ref.: #69480]	flagellated	no	91.251	yes
[Ref.: #69480]	gram-positive	no	93.835	yes
[Ref.: #69480]	anaerobic	yes	99.176	yes
[Ref.: #69480]	aerobic	no	98.287	yes
[Ref.: #69480]	halophile	no	90.966	no
[Ref.: #69480]	spore-forming	no	89.71	yes
[Ref.: #69480]	glucose-util	yes	89.328	no
[Ref.: #69480]	thermophile	yes	100	yes
[Ref.: #69480]	motile	yes	74.192	yes
[Ref.: #69480]	glucose-ferment	yes	55.728	no

Availability in culture collections External links

[Ref.: #6645]

Culture collection no.

DSM 16923, CCUG 50214

[Ref.: #86051]

SI-ID 297272

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Petrotoga halophila sp. nov., a thermophilic, moderately halophilic, fermentative bacterium isolated from an offshore oil well in Congo.	Miranda-Tello E, Fardeau ML, Joulian C, Magot M, Thomas P, Tholozan JL, Ollivier B	Int J Syst Evol Microbiol	10.1099/ijs.0.64516-0	2007	*

References

#6645

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16923

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31847

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28111 (see below)

#58792 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 50214

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#86051

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID297272.1 )

#28111

IJSEM 40 2007 ( DOI 10.1099/ijs.0.64516-0 , PubMed 17220438 )

* These data were automatically processed and therefore are not curated

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Petrotoga halophila DSM 16923 DSM 16923

Petrotoga halophila DSM 16923 DSM 16923

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