Actinoallomurus spadix (DSM 43459, ATCC 27298, BCRC 13386)

Name: Actinoallomurus spadix (DSM 43459, ATCC 27298, BCRC 13386)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17006

Type strain

Strain Designation FYU A 116, A-116

Culture col. no. DSM 43459 ATCC 27298 BCRC 13386 CBS 261.72 CIP 105479 18 more

NCBI tax ID(s) 79912

Special Collections DSMZ JCM KCTC CIP Wink compendium JMRC

History DSMZ <- M. Goodfellow <- KCC <- H. Nonomura, FYU, A 116 JCM <-- KCC A-0146 <-- H. Nonomura A-116. KCTC <- NCIMB <- H Nonomura A-116 CIP <- 1998, JCM <- KCC: strain A-0146 <- H. Nonomura: strain A-116

Links

Actinoallomurus spadix FYU A 116 is an aerobe, Gram-positive bacterium that was isolated from soil.

Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 389340

ATCC 27298,NRRL B-16128,CIP 105479,CCRC 13386,DSM 43459,IFO 14099,IMET 9752,NCIMB 11118,JCM 3146,NCIB 11118,CBS 261.72,KCTC 9252,NBRC 14099,BCRC 13386

@ref 20215

Last LPSN update 2026-05-29 08:28:54

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Thermomonosporaceae

Genus Actinoallomurus

Species Actinoallomurus spadix

Full scientific name Actinoallomurus spadix (Nonomura and Ohara 1971) Tamura et al. 2009

Synonyms (1)

Actinomadura spadix

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
67771	positive
122686	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11035	ISP2 MEDIUM (DSMZ Medium 987)	Medium recipe at MediaDive	Name: ISP 2 MEDIUM (DSMZ Medium 987) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Dextrose 4.0 g/l Yeast extract 4.0 g/l Distilled water
32726	MEDIUM 116 - for Streptomyces, Nocardia, Streptosporangium and Mycobacterium chlorophenolicum		Distilled water make up to (1000.000 ml);Agar (20.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g)
11035	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
122686	CIP Medium 194	Medium recipe at CIP
122686	CIP Medium 116	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
11035	positive	growth	37
18494	positive	optimum	28
32726	positive	growth	37
67770	positive	growth	37
67771	positive	growth	37
122686	positive	growth	15-41
122686	negative	growth	5

Physiology and metabolism

Oxygen tolerance

67771

Oxygen toleranceaerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
18494	NaCl	positive	maximum	2.5 %
122686	NaCl	positive	growth	0 %
122686	NaCl		growth	2 %
122686	NaCl		growth	4 %
122686	NaCl		growth	6 %
122686	NaCl		growth	8 %
122686	NaCl		growth	10 %

Observation

@ref	Observation
67770	quinones: MK-9(H₈), MK-9(H₆), MK-9(H₄)
67771	quinones: MK-9(H_8_), MK-9(H_6_), MK-9(H_4_)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
18494	22599 ChEBI	arabinose	+/-
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
18494	62968 ChEBI	cellulose	+/-
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
122686	4853 ChEBI	esculin	+	hydrolysis
18494	28757 ChEBI	fructose	+/-
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
18494	17234 ChEBI	glucose	+
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68368	25094 ChEBI	lysine	-	degradation	from API 20E
18494	29864 ChEBI	mannitol	+/-
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
18494	17268 ChEBI	myo-inositol	+/-
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
122686	17632 ChEBI	nitrate	+	reduction
122686	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
18494	16634 ChEBI	raffinose	+/-
18494	26546 ChEBI	rhamnose	+/-
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
18494	17992 ChEBI	sucrose	+/-
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
18494	18222 ChEBI	xylose	+/-

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
122686	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
122686	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test	Indole test
122686	15688 ChEBI	acetoin	-
68368	15688 ChEBI	acetoin	+			from API 20E
122686	17234 ChEBI	glucose		-
68368	35581 ChEBI	indole			-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
122686	alcohol dehydrogenase	-	1.1.1.1
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
122686	amylase	-
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
122686	beta-galactosidase	-	3.2.1.23
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
122686	caseinase	+	3.4.21.50
122686	catalase	+	1.11.1.6
68382	esterase (C 4)	-		from API zym
122686	gamma-glutamyltransferase	-	2.3.2.2
122686	gelatinase	-
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
122686	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
122686	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
122686	oxidase	-
122686	phenylalanine ammonia-lyase	-	4.3.1.24
122686	tryptophan deaminase	-
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
122686	urease	-	3.5.1.5
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	-		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
18494	-	-	-	-	-	-	-	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18494	not determinedn.d.	+	-	+	-	+	-	+	+	-	+	+	+	+	-	+	-	+	-	-
122686	-	-	-	-	-	+	-	-	-	-	+	+	-	-	-	+	-	+	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
122686	not determinedn.d.	+/-	-	+/-	+/-	+/-	+/-	-	+/-	-	+/-	+/-	+/-	+/-	-	+/-	-	-	+/-	-	-	-	-	-	-	+/-	-	+/-	+/-	+/-	+/-	+/-	+/-	-	-	+/-	+/-	+/-	+/-	+/-	+/-	+/-	-	-	+/-	-	-	-	-	+/-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11035	soil		Japan	JPN	Asia
67770	Soil, dry-heated	Yamanashi Pref.	Japan	JPN	Asia
67771	From soil , dry-heated	Yamanashi Pref.	Japan	JPN	Asia
122686	Environment, Soil	Yamanashi	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AJ293712 (>99% sequence identity) for Actinoallomurus spadix subclade from Microbeatlas

Aquatic Samples	6
Soil Samples	392
Animal Samples	26
Plant Samples	2
Total Samples	426

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11035	1	Risk group (German classification) According to TRBA 466
18494	1	German classification According to TRBA 466
122686	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2405572v1 assembly for Actinoallomurus spadix KCTC 9252	contig	GCA_024055725	79912.4	8045838998	79912	58.01
124043	ASM3952098v1 assembly for Actinoallomurus spadix JCM 3146	contig	GCA_039520985			79912	47.63

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Actinomadura spadix strain ATCC27298 16S ribosomal RNA gene, partial sequence	AF051380	118	79912
20218	Actinomadura spadix 16S ribosomal RNA gene, partial sequence	AF163120	1458	79912
20218	Actinomadura spadix 16S-23S ribosomal RNA intergenic spacer sequence	AF163131	395	79912
11035	Actinoallomurus spadix gene for 16S rRNA, partial sequence, strain: NBRC 14099	AB364581	1449	79912
67770	Actinomadura spadix partial 16S rRNA gene, strain IMSNU 20015T	AJ293712	1488	79912
124043	Actinoallomurus spadix strain JCM 3146 16S ribosomal RNA gene, partial sequence.	MT760418	1378	79912

GC content

@ref	GC-content (mol%)	Method
11035	74
67771	66.4	thermal denaturation, midpoint method (Tm)
67770	74	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinoallomurus spadix KCTC 9252
NCBI: GCA_024055725.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.25	yes
125438	anaerobic	anaerobicⓘ	no	90.31	yes
125438	aerobic	aerobicⓘ	yes	87.76	yes
125438	spore-forming	spore-formingⓘ	yes	89.33	no
125438	thermophilic	thermophileⓘ	no	96.25	yes
125438	flagellated	motile2+ⓘ	no	88.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Metagenomics-based exploration of key soil microorganisms contributing to continuously planted Casuarina equisetifolia growth inhibition and their interactions with soil nutrient transformation.	Wang Y, Lin S, Li J, Jia X, Hu M, Cai Y, Cheng P, Li M, Chen Y, Lin W, Wang H, Wu Z.	Front Plant Sci	10.3389/fpls.2023.1324184	2023
Phylogeny	Characterization of bacterial community structure during in-vessel composting of agricultural waste by 16S rRNA sequencing.	Varma VS, Dhamodharan K, Kalamdhad AS.	3 Biotech	10.1007/s13205-018-1319-7	2018
	Actinoallomurus soli sp. nov. and Actinoallomurus rhizosphaericola sp. nov., two novel actinobacteria isolated from rhizosphere soil of Oryza rufipogon Griff.	Chantavorakit T, Muangham S, Aaron TWF, Duangmal K, Hong K.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006177	2023
Phylogeny	Actinoallomurus acanthiterrae sp. nov., an actinomycete isolated from rhizosphere soil of the mangrove plant Acanthus ilicifolius.	Tang YL, Lin HP, Xie QY, Li L, Peng F, Deng Z, Hong K	Int J Syst Evol Microbiol	10.1099/ijs.0.043380-0	2012
Phylogeny	Transfer of Actinomadura spadix Nonomura and Ohara 1971 to Actinoallomurus spadix gen. nov., comb. nov., and description of Actinoallomurus amamiensis sp. nov., Actinoallomurus caesius sp. nov., Actinoallomurus coprocola sp. nov., Actinoallomurus fulvus sp. nov., Actinoallomurus iriomotensis sp. nov., Actinoallomurus luridus sp. nov., Actinoallomurus purpureus sp. nov. and Actinoallomurus yoronensis sp. nov.	Tamura T, Ishida Y, Nozawa Y, Otoguro M, Suzuki K	Int J Syst Evol Microbiol	10.1099/ijs.0.006858-0	2009

References

#11035	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43459
#18494	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#32726	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68368	Automatically annotated from API 20E .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#122686	Collection of Institut Pasteur ; Curators of the CIP; CIP 105479
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Actinoallomurus spadix (DSM 43459, ATCC 27298, BCRC 13386)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Observation

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API 20E

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_024055725

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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