Actinomadura geliboluensis (DSM 45508, KCTC 19868, A8036)

Name: Actinomadura geliboluensis (DSM 45508, KCTC 19868, A8036)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17005

Type strain

Strain Designation A8036

Culture col. no. DSM 45508 KCTC 19868 A8036 JCM 19952

NCBI tax ID(s) 882440

Special Collections DSMZ JCM KCTC Wink compendium Literature strains

History DSMZ <- N. Sahin, Ondokuz Mayis Univ., Dept. of Biology, Kurupelit-Samsun, Turkey; A8036 JCM <-- KCTC 19868 <-- N. Sahin A8036. KCTC <- Nevzat Sahin, Ondokuz Mayis Univ., Turkey

Links

Actinomadura geliboluensis A8036 is an aerobe, spore-forming, Gram-positive bacterium that builds an aerial mycelium and was isolated from soil.

spore-forming Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 407716

KCTC 19868,DSM 45508

@ref 20215

Last LPSN update 2026-05-29 08:28:54

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Thermomonosporaceae

Genus Actinomadura

Species Actinomadura geliboluensis

Full scientific name Actinomadura geliboluensis Sazak et al. 2012

Morphology

Cell morphology

@ref	Gram stain	Motility
30440	positive
67771	positive

Colony morphology

@ref	Colony color	Medium used
69400	Ivory (1014)	ISP 2
69400	Ivory (1014)	ISP 4
69400	Ivory (1014)	ISP 5
69400	Ivory (1014), light ivory (1015)	ISP 7
69400	Sand yellow (1002)	ISP 6
69400	Sand yellow (1002), grey beige (1019)	suter with tyrosine
69400	Yellow grey (7034)	suter without tyrosine

Multicellular morphology

@ref	Complex name	Complex color	Medium name
69400	Aerial mycelium	Signal white (9003)	ISP 2
69400	Aerial mycelium	Pure white (9010)	ISP 3
69400	Aerial mycelium	Traffic white (9016)	ISP 4
69400	Aerial mycelium		ISP 5
69400	Aerial mycelium		ISP 6
69400	Aerial mycelium		ISP 7
69400	Aerial mycelium		suter with tyrosine
69400	Aerial mycelium		suter without tyrosine

Pigmentation

@ref	Production	Name
30440
69400		soluble pigment

Multimedia

@ref:	69400
multimedia content:	DSM_45508_image3.jpeg
multimedia.multimedia content:	DSM_45508_image3.jpeg
caption:	Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)
intellectual property rights:	Helmholtz-Zentrum für Infektionsforschung GmbH
manual_annotation:	1

@ref:	69400
multimedia content:	DSM_45508_image4.jpeg
multimedia.multimedia content:	DSM_45508_image4.jpeg
caption:	Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)
intellectual property rights:	Helmholtz-Zentrum für Infektionsforschung GmbH
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
17455	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
17455	GPHF-MEDIUM (DSMZ Medium 553)	Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
17455	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
17455	positive	growth	28
30440	positive	growth	20-45
30440	positive	optimum	30
67770	positive	growth	28
67771	positive	growth	28

Culture pH

@ref	Ability	Type	PH	PH range
30440	positive	optimum	7
30440	positive	growth	05-11	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
30440	aerobe
67771	aerobe

Spore formation

30440

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
30440	NaCl	positive	growth	0-5 %
69400	NaCl	positive	growth	0-2.5 %

Observation

@ref	Observation
67770	quinones: MK-9(H₆), MK-9(H₄), MK-9(H₈)
67771	quinones: MK-9(H6)(61 %), MK-9(H4)(21%), MK-9(H8)(8%), MK-9(H2)(3%)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
30440	22599 ChEBI	arabinose	+	carbon source
69400	22599 ChEBI	arabinose	+	growth
30440	17057 ChEBI	cellobiose	+	carbon source
69400	62968 ChEBI	cellulose	+/-	growth
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
30440	23652 ChEBI	dextrin	+	carbon source
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
30440	28757 ChEBI	fructose	+	carbon source
69400	28757 ChEBI	fructose	+	growth
30440	28260 ChEBI	galactose	+	carbon source
69400	17234 ChEBI	glucose	+	growth
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
30440	17716 ChEBI	lactose	+	carbon source
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
30440	17306 ChEBI	maltose	+	carbon source
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
30440	29864 ChEBI	mannitol	+	carbon source
69400	37684 ChEBI	mannose	+/-	growth
30440	28053 ChEBI	melibiose	+	carbon source
30440	17268 ChEBI	myo-inositol	+	carbon source
69400	17268 ChEBI	myo-inositol	+/-	growth
30440	17632 ChEBI	nitrate	+	reduction
68379	17632 ChEBI	nitrate	+	reduction	from API Coryne
69400	16634 ChEBI	raffinose	-	growth
30440	26546 ChEBI	rhamnose	+	carbon source
69400	26546 ChEBI	rhamnose	+	growth
30440	15963 ChEBI	ribitol	+	carbon source
30440	30911 ChEBI	sorbitol	+	carbon source
30440	30031 ChEBI	succinate	+	carbon source
30440	17992 ChEBI	sucrose	+	carbon source
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
69400	17992 ChEBI	sucrose	+/-	growth
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
30440	18222 ChEBI	xylose	+	carbon source
69400	18222 ChEBI	xylose	+	growth

Enzymes

@ref	Value	Activity	Ec
30440	acid phosphatase	+	3.1.3.2
68382	acid phosphatase	+	3.1.3.2	from API zym
30440	alkaline phosphatase	+	3.1.3.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
30440	catalase	+	1.11.1.6
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
68379	urease	-	3.5.1.5	from API Coryne
68382	valine arylamidase	+		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
69400	+	-	-	+	-	-	+	-	+	-	+/-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
69400	not determinedn.d.	+	+/-	+/-	-	+	+	+/-	-	+	+	+	-	-	-	+	+	+/-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
17455	soil	Canakkale, Gelibolu	Turkey	TUR
67770	Soil	Canakkale	Turkey	TUR	Asia
67771	From soil	Canakkale	Turkey	TUR	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
17455	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM588974v1 assembly for Actinomadura geliboluensis A8036	contig	GCA_005889745	882440.3	8112038168	882440	0

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17455	Actinomadura geliboluensis strain A8036 16S ribosomal RNA gene, partial sequence	HQ157187	1490		882440

GC content

@ref	GC-content (mol%)	Method
17455	70.8	fluorimetric
30440	70.8
67770	70.8	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinomadura geliboluensis A8036
NCBI: GCA_005889745.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	86.85	no
125438	anaerobic	anaerobicⓘ	no	88.45	yes
125438	aerobic	aerobicⓘ	yes	89.66	yes
125438	spore-forming	spore-formingⓘ	yes	89.43	yes
125438	thermophilic	thermophileⓘ	no	91.00	yes
125438	flagellated	motile2+ⓘ	no	89.10	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	A sensitive pH indicator-based spectrophotometric assay for PHB depolymerase activity on microtiter plates.	Camacho-Ruiz MA, Muller-Santos M, Hernandez-Mancillas XD, Armenta-Perez VP, Zamora-Gonzalez E, Rodriguez JA	Anal Methods	10.1039/d0ay00840k	2020
Phylogeny	Actinomadura soli sp. nov., isolated from the top soil layer on basaltic material in Turkey.	Saricaoglu S, Saygin H, Topkara AR, Gencbay T, Guven K, Cetin D, Sahin N, Isik K	Int J Syst Evol Microbiol	10.1099/ijsem.0.005062	2021
Phylogeny	Actinomadura geliboluensis sp. nov., isolated from soil.	Sazak A, Camas M, Sproer C, Klenk HP, Sahin N	Int J Syst Evol Microbiol	10.1099/ijs.0.036145-0	2011

References

#17455	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45508
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26776	IJSEM 2011 2012 ( DOI 10.1099/ijs.0.036145-0 , PubMed 22021584 )
#30440	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26776
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69400	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinomadura geliboluensis (DSM 45508, KCTC 19868, A8036)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_005889745

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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