Name: Actinomadura geliboluensis A8036
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17005

Type strain:

Species: Actinomadura geliboluensis

Strain Designation: A8036

Culture col. no.: DSM 45508, KCTC 19868, JCM 19952

Strain history: <- Nevzat Sahin, Ondokuz Mayis Univ., Turkey

NCBI tax ID(s): 882440 (species)

SI-ID 407716

DSM 45508

Section

Actinomadura geliboluensis A8036 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

Gram-positive
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Thermomonosporaceae*
	Genus	*Actinomadura*
	Species	**Actinomadura geliboluensis**
	Full Scientific Name (LPSN)	Actinomadura geliboluensis Sazak et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #30440]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #30440]	Motility	no

[Ref.: #69400]	Colony color	Ivory (1014)
[Ref.: #69400]	Cultivation medium used	ISP 2

[Ref.: #69400]	Colony color	Ivory (1014)
[Ref.: #69400]	Cultivation medium used	ISP 4

[Ref.: #69400]	Colony color	Ivory (1014)
[Ref.: #69400]	Cultivation medium used	ISP 5

[Ref.: #69400]	Colony color	Ivory (1014), light ivory (1015)
[Ref.: #69400]	Cultivation medium used	ISP 7

[Ref.: #69400]	Colony color	Sand yellow (1002)
[Ref.: #69400]	Cultivation medium used	ISP 6

[Ref.: #69400]	Colony color	Sand yellow (1002), grey beige (1019)
[Ref.: #69400]	Cultivation medium used	suter with tyrosine

[Ref.: #69400]	Colony color	Yellow grey (7034)
[Ref.: #69400]	Cultivation medium used	suter without tyrosine

[Ref.: #30440]	Pigment production	no
[Ref.: #69400]	Pigment production	no
[Ref.: #69400]	Pigment name	soluble pigment

[Ref.: #69400]	Multicellular complex forming ability	yes
[Ref.: #69400]	Multicellular complex name	Aerial mycelium
[Ref.: #69400]	Multicellular complex color	Signal white (9003)
[Ref.: #69400]	Medium Name (multicellularity)	ISP 2

[Ref.: #69400]	Multicellular complex forming ability	yes
[Ref.: #69400]	Multicellular complex name	Aerial mycelium
[Ref.: #69400]	Multicellular complex color	Pure white (9010)
[Ref.: #69400]	Medium Name (multicellularity)	ISP 3

[Ref.: #69400]	Multicellular complex forming ability	yes
[Ref.: #69400]	Multicellular complex name	Aerial mycelium
[Ref.: #69400]	Multicellular complex color	Traffic white (9016)
[Ref.: #69400]	Medium Name (multicellularity)	ISP 4

[Ref.: #69400]	Multicellular complex forming ability	no
[Ref.: #69400]	Multicellular complex name	Aerial mycelium
[Ref.: #69400]	Medium Name (multicellularity)	ISP 5

[Ref.: #69400]	Multicellular complex forming ability	no
[Ref.: #69400]	Multicellular complex name	Aerial mycelium
[Ref.: #69400]	Medium Name (multicellularity)	ISP 6

[Ref.: #69400]	Multicellular complex forming ability	no
[Ref.: #69400]	Multicellular complex name	Aerial mycelium
[Ref.: #69400]	Medium Name (multicellularity)	ISP 7

[Ref.: #69400]	Multicellular complex forming ability	yes
[Ref.: #69400]	Multicellular complex name	Aerial mycelium
[Ref.: #69400]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69400]	Multicellular complex forming ability	no
[Ref.: #69400]	Multicellular complex name	Aerial mycelium
[Ref.: #69400]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #69400]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69400]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69400]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69400]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17455]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #17455]

Culture medium growth

[Ref.: #17455]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #17455]

Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

[Ref.: #17455]

Culture medium growth

[Ref.: #17455]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #17455]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #17455]

Culture medium growth

[Ref.: #17455]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17455]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #17455]	growth	28 °C
[Ref.: #30440]	growth	20-45 °C
[Ref.: #30440]	optimum	30 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	28 °C

[Ref.: #17455]	Temperature range	mesophilic
[Ref.: #30440]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30440]			optimum	7
[Ref.: #30440]			growth	05-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30440]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #30440]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30440]		NaCl		growth	0-5	%
[Ref.: #69400]		NaCl		growth	0-2.5	%

[Ref.: #67770]	Observation	quinones: MK-9(H₆), MK-9(H₄), MK-9(H₈)
[Ref.: #67771]	Observation	quinones: MK-9(H6)(61 %), MK-9(H4)(21%), MK-9(H8)(8%), MK-9(H2)(3%)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30440]	arabinose ChEBI	+	carbon source
[Ref.: #69400]	arabinose ChEBI	+	growth
[Ref.: #30440]	cellobiose ChEBI	+	carbon source
[Ref.: #69400]	cellulose ChEBI	+/-	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30440]	dextrin ChEBI	+	carbon source
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #30440]	fructose ChEBI	+	carbon source
[Ref.: #69400]	fructose ChEBI	+	growth
[Ref.: #30440]	galactose ChEBI	+	carbon source
[Ref.: #69400]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69400]	inositol ChEBI	+/-	growth
[Ref.: #30440]	lactose ChEBI	+	carbon source
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30440]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30440]	mannitol ChEBI	+	carbon source
[Ref.: #69400]	mannose ChEBI	+/-	growth
[Ref.: #30440]	melibiose ChEBI	+	carbon source
[Ref.: #30440]	myo-inositol ChEBI	+	carbon source
[Ref.: #30440]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	+	reduction	* from API Coryne
[Ref.: #69400]	raffinose ChEBI	-	growth
[Ref.: #30440]	rhamnose ChEBI	+	carbon source
[Ref.: #69400]	rhamnose ChEBI	+	growth
[Ref.: #30440]	ribitol ChEBI	+	carbon source
[Ref.: #30440]	sorbitol ChEBI	+	carbon source
[Ref.: #30440]	succinate ChEBI	+	carbon source
[Ref.: #30440]	sucrose ChEBI	+	carbon source
[Ref.: #69400]	sucrose ChEBI	+/-	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #30440]	xylose ChEBI	+	carbon source
[Ref.: #69400]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #30440]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #30440]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #30440]	catalase	+	1.11.1.6
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69400]	3893	+	-	-	+	-	-	+	-	+	-	+/-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69400]	6077	not determinedn.d.	+	+/-	+/-	-	+	+	+/-	-	+	+	+	-	-	-	+	+	+/-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17455]	Sample type/isolated from	soil
[Ref.: #17455]	Geographic location (country and/or sea, region)	Canakkale, Gelibolu
[Ref.: #17455]	Country	Turkey
[Ref.: #17455]	Country ISO 3 Code	TUR

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Canakkale
[Ref.: #67770]	Country	Turkey
[Ref.: #67770]	Country ISO 3 Code	TUR
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From soil
[Ref.: #67771]	Geographic location (country and/or sea, region)	Canakkale
[Ref.: #67771]	Country	Turkey
[Ref.: #67771]	Country ISO 3 Code	TUR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17455]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17455]	Actinomadura geliboluensis strain A8036 16S ribosomal RNA gene, partial sequence	HQ157187	1490	ENA	882440 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinomadura geliboluensis A8036	GCA_005889745	contig	GenBank	882440 tax ID	*

[Ref.: #17455]	GC-content	70.8 mol%	fluorimetric
[Ref.: #30440]	GC-content	70.8 mol%
[Ref.: #67770]	GC-content	70.8 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinomadura geliboluensis A8036 NCBI: GCA_005889745.1
[Ref.: #69480]	motile	no	90.383	no
[Ref.: #69480]	flagellated	no	97.585	yes
[Ref.: #69480]	gram-positive	yes	88.257	yes
[Ref.: #69480]	anaerobic	no	97.936	yes
[Ref.: #69480]	aerobic	yes	86.722	no
[Ref.: #69480]	halophile	no	88.197	no
[Ref.: #69480]	spore-forming	yes	92.125	yes
[Ref.: #69480]	thermophile	no	96.154	yes
[Ref.: #69480]	glucose-util	yes	88.14	no
[Ref.: #69480]	glucose-ferment	no	90.407	yes

Availability in culture collections External links

[Ref.: #17455]

Culture collection no.

DSM 45508, KCTC 19868, JCM 19952

[Ref.: #86006]

SI-ID 407716

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	A sensitive pH indicator-based spectrophotometric assay for PHB depolymerase activity on microtiter plates.	Camacho-Ruiz MA, Muller-Santos M, Hernandez-Mancillas XD, Armenta-Perez VP, Zamora-Gonzalez E, Rodriguez JA	Anal Methods	10.1039/d0ay00840k	2020	*
Phylogeny	Actinomadura soli sp. nov., isolated from the top soil layer on basaltic material in Turkey.	Saricaoglu S, Saygin H, Topkara AR, Gencbay T, Guven K, Cetin D, Sahin N, Isik K	Int J Syst Evol Microbiol	10.1099/ijsem.0.005062	2021	*
Phylogeny	Actinomadura geliboluensis sp. nov., isolated from soil.	Sazak A, Camas M, Sproer C, Klenk HP, Sahin N	Int J Syst Evol Microbiol	10.1099/ijs.0.036145-0	2011	*

References

#17455

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45508

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30440

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26776 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69400

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#86006

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407716.1 )

#26776

IJSEM 2011 2012 ( DOI 10.1099/ijs.0.036145-0 , PubMed 22021584 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Actinomadura geliboluensis in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF