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Culture medium |
GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | |
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[Ref.: #15650] |
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Culture medium link |
Medium recipe at MediaDive |
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Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
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[Ref.: #20178] |
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Name: ISP 2 / Yeast Malt Agar (5265); 5265
Composition
Malt extract 10.0 g/l
Yeast extract 4.0 g/l
Glucose 4.0 g/l
Agar 15.0 g/l
Preparation:
Sterilisation: 20 minutes at 121°C
pH before sterilisation: 7.0
Usage: Maintenance and Taxonomy
Organisms: All Actinomycetes |
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[Ref.: #20178] |
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Name: ISP 3; 5315
Composition
Dog oat flakes 20.0 g/l
Trace element solution (5314) 2.5 ml/l
Agar 18.0 g/l
Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated).
Sterilisation: 20 minutes at 121°C
pH before sterilisation: 7.8
Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production)
Organisms: All Actinomycetes
Trace element solution 5314
Name: Trace element solution 5314; 5314
Composition
CaCl2 x H2O 3.0 g/l
Fe-III-citrate 1.0 g/l
MnSO4 0.2 g/l
ZnCl2 0.1 g/l
CuSO4 x 5 H2O 0.025 g/l
Sodium tetra borate 0.2 g/l
CoCl2 x 6 H2O 0.004 g/l
Sodium molybdate 0.01 g/l
Preparation: Use double destillated water.
Sterilisation: 20 minutes at 121°C
pH before sterilisation:
Usage: Trace element solution for different media
Organisms: |
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[Ref.: #20178] |
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Name: ISP 4; DSM 547
Solution I:
Difco soluble starch, 10.0 g.
Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml.
Solution II:
CaCO3 2.0 g
K2HPO4 (anhydrous) 1.0 g
MgSO4 x 7 H2O 1.0 g
NaCl 1.0 g
(NH4)2SO4 2.0 g
Distilled water 500.0 ml
Trace salt solution (see below) 1.0 ml
The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min.
Trace element solution:
FeSO4 x 7 H2O 0.1 g
MnCl2 x 4 H2O 0.1 g
ZnSO4 x 7 H2O 0.1 g
Distilled water 100.0 ml |
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[Ref.: #20178] |
Culture medium |
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[Ref.: #20178] |
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[Ref.: #20178] |
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Name: ISP 5 (5323)
Composition
L-Asparagine 1.0 g/l
Glycerol 10.0 g/l
K2HPO4 1.0 g/l
Salt solution (see preparation) 1.0 ml/l
Agar 20.0 g/l
Preparation:
Salt solution
1.0 g FeSO4 x 7 H2O
1.0 g MnCl2 x 4 H2O
1.0 g ZNSO4 x 7 H2O
in 100 ml water
Sterilisation:
20 minutes at 121°C
pH before sterilisation:
7.2
Usage:
Maintenance and taxonomy
Organisms:
All Actinomycetes |
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[Ref.: #20178] |
Culture medium |
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[Ref.: #20178] |
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Name: ISP 6 (5318)
Composition
Peptone 15.0 g/l
Proteose peptose 5.0 g/l
Ferric ammonium citrate 0.5 g/l
Sodium glycerophosphate 1.0 g/l
Sodium thiosulfate 0.08 g/l
Yeast extract 1.0 g/l
Agar 15.0 g/l
Sterilisation:
20 minutes at 121°C
pH before sterilisation:
Usage: Production of melanoid pigments
Organisms:
All Actinomycetes |
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Name: ISP 7 (5322)
Composition
Glycerol 15.0 g/l
L-Tyrosine 0.5 g/l
L-Asparagine 1.0 g/l
K2HPO4 0.5 g/l
NaCl 0.5 g/l
FeSO4 x 7 H2O 0.01 g/l
Trace element solution 5343 1.0 ml/l
Agar 20.0
Sterilisation:
20 minutes at 121°C
pH before sterilisation:
7.3
Usage:
Production of melanoid pigments
Organisms:
All Actinomycetes |
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[Ref.: #15650] |
Culture medium |
STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) | |
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[Ref.: #15650] |
Culture medium growth |
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[Ref.: #15650] |
Culture medium link |
Medium recipe at MediaDive |
[Ref.: #15650] |
Culture medium composition |
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Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water |
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[Ref.: #15650] |
Culture medium |
GPHF-MEDIUM (DSMZ Medium 553) | |
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[Ref.: #15650] |
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[Ref.: #15650] |
Culture medium link |
Medium recipe at MediaDive |
[Ref.: #15650] |
Culture medium composition |
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Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water |
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[Ref.: #15650] |
Culture medium |
ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | |
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Medium recipe at MediaDive |
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Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water |
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[Ref.: #15650] |
Culture medium |
TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | |
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[Ref.: #15650] |
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Medium recipe at MediaDive |
[Ref.: #15650] |
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Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |