Actinomadura rudentiformis (DSM 44962, JCM 14907, NRRL B-24458)

Name: Actinomadura rudentiformis (DSM 44962, JCM 14907, NRRL B-24458)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16991

Type strain

Strain Designation HMC 1

Culture col. no. DSM 44962 JCM 14907 NRRL B-24458 HMC 1

NCBI tax ID(s) 359158

Special Collections DSMZ JCM Wink compendium Literature strains

History <- P. R. Meyers; HMC 1 <- M. le Roes DSM 44962 <-- P. R. Meyers HMC1 <-- M. le Roes.

Links

Actinomadura rudentiformis HMC 1 is an aerobe, Gram-positive bacterium that was isolated from soil.

Gram-positive aerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 297112

DSM 44962,NRRL B-24458,JCM 14907

@ref 20215

Last LPSN update 2026-02-09 11:22:06

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Thermomonosporaceae

Genus Actinomadura

Species Actinomadura rudentiformis

Full scientific name Actinomadura rudentiformis Le Roes and Meyers 2007

Morphology

Cell morphology

31825

Gram stainpositive

Colony morphology

@ref	Colony color	Incubation period	Medium used
20146	Brown beige (1011)	10-14 days	ISP 2
20146	Beige (1001)	10-14 days	ISP 3
20146	Beige (1001)	10-14 days	ISP 4
20146	Beige (1001)	10-14 days	ISP 5
20146	Beige (1001)	10-14 days	ISP 6
20146	Beige (1001)	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
20146		ISP 2
20146		ISP 3
20146		ISP 4
20146		ISP 5
20146		ISP 6
20146		ISP 7

Pigmentation

31825

Productionno

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
12215	N-Z-AMINE-MEDIUM (DSMZ Medium 554)	Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
12215	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
12215	GPHF-MEDIUM (DSMZ Medium 553)	Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
20146	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20146	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20146	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20146	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20146	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20146	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)
12215	positive	growth	28
20146	positive	optimum	28
31825	positive	growth	30-45
31825	positive	optimum	37.5
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

12215

Oxygen toleranceaerobe

Spore formation

31825

Spore formationno

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31825	30089 ChEBI	acetate	+	carbon source
31825	22599 ChEBI	arabinose	+	carbon source
31825	29016 ChEBI	arginine	+	carbon source
31825	17057 ChEBI	cellobiose	+	carbon source
31825	4853 ChEBI	esculin	+	hydrolysis
31825	28757 ChEBI	fructose	+	carbon source
31825	28260 ChEBI	galactose	+	carbon source
31825	17234 ChEBI	glucose	+	carbon source
31825	27570 ChEBI	histidine	+	carbon source
31825	17716 ChEBI	lactose	+	carbon source
31825	29864 ChEBI	mannitol	+	carbon source
31825	37684 ChEBI	mannose	+	carbon source
31825	28053 ChEBI	melibiose	+	carbon source
31825	17268 ChEBI	myo-inositol	+	carbon source
31825	28044 ChEBI	phenylalanine	+	carbon source
31825	16634 ChEBI	raffinose	+	carbon source
31825	26546 ChEBI	rhamnose	+	carbon source
31825	15963 ChEBI	ribitol	+	carbon source
31825	33942 ChEBI	ribose	+	carbon source
31825	17814 ChEBI	salicin	+	carbon source
31825	17822 ChEBI	serine	+	carbon source
31825	17992 ChEBI	sucrose	+	carbon source
31825	26986 ChEBI	threonine	+	carbon source
31825	27082 ChEBI	trehalose	+	carbon source
31825	18222 ChEBI	xylose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
31825	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
31825	gelatinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68382	trypsin	+	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20146	not determinedn.d.	+	+	+	-	+	+	+	+	+	+	+	+	+	-	-	+	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
12215	soil	Swartberg nature reserve	South Africa	ZAF	Africa
67770	Soil from the banks of the Gamka River in the Swartberg Nature Reserve	Western Cape Province	South Africa	ZAF	Africa

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
12215	1	Risk group (German classification) According to TRBA 466
20146	1	German classification According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
12215	Actinomadura rudentiformis strain HMC1 16S ribosomal RNA gene, partial sequence	DQ285420	1433		359158

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinomadura rudentiformis sp. nov., isolated from soil.	le Roes M, Meyers PR	Int J Syst Evol Microbiol	10.1099/ijs.0.64438-0	2007

References

#12215	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44962
#20146	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28091	IJSEM 45 2007 ( DOI 10.1099/ijs.0.64438-0 , PubMed 17220439 )
#31825	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28091
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinomadura rudentiformis (DSM 44962, JCM 14907, NRRL B-24458)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

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