Actinomadura cremea subsp. cremea (DSM 43676, ATCC 33577, INA 292)

Name: Actinomadura cremea subsp. cremea (DSM 43676, ATCC 33577, INA 292)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16927

Type strain

Culture col. no. DSM 43676 ATCC 33577 INA 292 VKM Ac-912 NRRL B-16605 6 more

NCBI tax ID(s) 1991 31961

Special Collections DSMZ JCM Wink compendium

History <- N.S. Agre, INA KCC A-0308 <-- IFO 14182 <-- ATCC 33577 <-- INA 292.

Links

Actinomadura cremea subsp. cremea DSM 43676 is a bacterium that builds an aerial mycelium.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 40568

ATCC 33577,IFO 14182,DSM 43676,JCM 3308,CCRC 13394,IMET 9578,NRRL B-16605,VKM Ac-912,NBRC 14182,BCRC 13394

@ref 20215

Last LPSN update 2026-02-09 11:22:05

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Thermomonosporaceae

Genus Actinomadura

Species Actinomadura cremea subsp. cremea

Full scientific name Actinomadura cremea subsp. cremea (Preobrazhenskaya et al. 1975) Gauze et al. 1987

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	positive	99.6

Colony morphology

@ref	Incubation period	Medium used
19579	10-14 days	ISP 2
19579	10-14 days	ISP 3
19579	10-14 days	ISP 4
19579	10-14 days	ISP 5
19579	10-14 days	ISP 6
19579	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19579			ISP 2
19579	Aerial Mycelium	White	ISP 3
19579	Aerial Mycelium		ISP 4
19579	Aerial Mycelium	Pink	ISP 5
19579	Aerial Mycelium	White	ISP 6
19579	Aerial Mycelium		ISP 7

Multimedia

@ref:	11239
multimedia content:	DSM_43676.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43676.jpg
caption:	Medium 553 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
19579	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19579	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19579	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19579	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19579	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19579	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
11239	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
11239	N-Z-AMINE-MEDIUM (DSMZ Medium 554)	Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
11239	GPHF-MEDIUM (DSMZ Medium 553)	Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
11239	positive	growth	28
19579	positive	optimum	28
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	aerobe	94.9

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19579	NaCl	positive	maximum	2.5 %

Observation

67770

Observationquinones: MK-9(H₆), MK-9(H₆), MK-9(H₄), MK-9(H₈)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19579	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
19579	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
19579	28757 ChEBI	fructose	-
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19579	17234 ChEBI	glucose	-
68368	25094 ChEBI	lysine	-	degradation	from API 20E
19579	29864 ChEBI	mannitol	-
19579	17268 ChEBI	myo-inositol	-
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
19579	16634 ChEBI	raffinose	-
19579	26546 ChEBI	rhamnose	-
19579	17992 ChEBI	sucrose	+
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19579	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68382	beta-galactosidase	-	3.2.1.23	from API zym
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	+	3.2.1.31	from API zym
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	+		from API zym
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68382	trypsin	-	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19579	-	-	-	+	-	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19579	not determinedn.d.	+	-	+	+	+	+	+	-	+	+	+	-	-	+	+	+	+	-	-

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
19579	1	Risk group (German classification) According to TRBA 466
11239	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID
66792	ASM1464849v1 assembly for Actinomadura cremea JCM 3308	scaffold	GCA_014648495	1991.3	2995954180	1991

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Actinomadura cremea subsp. cremea strain ATCC33577 16S ribosomal RNA gene, partial sequence	AF051379	118	31961
20218	Actinomadura cremea subsp. cremea 16S-23S ribosomal RNA intergenic spacer region, partial sequence	AF134094	397	31961
20218	Actinomadura cremea subsp. cremea gene for 16S ribosomal RNA, partial sequence	AB462291	1436	31961
11239	Actinomadura cremea subsp. cremea 16S ribosomal RNA gene, partial sequence	AF134067	1432	31961
124043	Actinomadura cremea strain JCM 3308 16S ribosomal RNA gene, partial sequence.	MT760442	1350	1991

GC content

@ref	GC-content (mol%)	Method
67770	68	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinomadura cremea JCM 3308
NCBI: GCA_014648495

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	85.60	no
125439	motility	BacteriaNetⓘ	no	83.70	no
125439	gram_stain	BacteriaNetⓘ	positive	99.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	94.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinomadura cremea JCM 3308
NCBI: GCA_014648495.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.97	no
125438	anaerobic	anaerobicⓘ	no	94.54	no
125438	aerobic	aerobicⓘ	yes	87.98	no
125438	spore-forming	spore-formingⓘ	yes	88.50	no
125438	thermophilic	thermophileⓘ	no	95.36	yes
125438	flagellated	motile2+ⓘ	no	89.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Culture and amplification-free nanopore sequencing for rapid detection of pathogens and antimicrobial resistance genes from urine.	Bellankimath AB, Chapagain C, Branders S, Ali J, Wilson RC, Johansen TEB, Ahmad R.	Eur J Clin Microbiol Infect Dis	10.1007/s10096-024-04929-1	2024
Genetics	Karakum desert: a unique source of cultivable novel and rare actinomycetes with a remarkable biosynthetic potential.	Saygin H, Sahin N, Goodfellow M.	World J Microbiol Biotechnol	10.1007/s11274-025-04399-3	2025
	Short turnaround time of seven to nine hours from sample collection until informed decision for sepsis treatment using nanopore sequencing.	Ali J, Johansen W, Ahmad R.	Sci Rep	10.1038/s41598-024-55635-z	2024
	One-Step Microbial Conversion of a Racemic Mixture of Pantoyl Lactone to Optically Active d-(-)-Pantoyl Lactone.	Shimizu S, Hattori S, Hata H, Yamada H.	Appl Environ Microbiol	10.1128/aem.53.3.519-522.1987	1987
Genetics	Structural features of cell wall teichoic acid and peptidoglycan of Actinomadura cremea INA 292.	Potekhina NV, Naumova IB, Shashkov AS, Terekhova LP	Eur J Biochem	10.1111/j.1432-1033.1991.tb16126.x	1991
Phylogeny	Majority of Actinomadura clinical isolates from sputa or bronchoalveolar lavage fluid in Japan belongs to the cluster of Actinomadura cremea and Actinomadura nitritigenes, and the description of Actinomadura chibensis sp. nov.	Hanafy A, Ito J, Iida S, Kang Y, Kogure T, Yazawa K, Yaguchi T, Mikami Y.	Mycopathologia	10.1007/s11046-006-0045-6	2006
Phylogeny	Actinomadura algeriensis sp. nov., an actinobacterium isolated from Saharan soil.	Lahoum A, Bouras N, Mathieu F, Schumann P, Sproer C, Klenk HP, Sabaou N	Antonie Van Leeuwenhoek	10.1007/s10482-015-0617-x	2015
Phylogeny	Actinomadura sediminis sp. nov., a marine actinomycete isolated from mangrove sediment.	He J, Xu Y, Sahu MK, Tian XP, Nie GX, Xie Q, Zhang S, Sivakumar K, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.032979-0	2011
Phylogeny	Actinomadura apis sp. nov., isolated from a honey bee (Apis mellifera) hive, and the reclassification of Actinomadura cremea subsp. rifamycini Gauze et al. 1987 as Actinomadura rifamycini (Gauze et al. 1987) sp. nov., comb. nov.	Promnuan Y, Kudo T, Ohkuma M, Chantawannakul P	Int J Syst Evol Microbiol	10.1099/ijs.0.026633-0	2010

References

#11239	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43676
#19579	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68382	Automatically annotated from API zym .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinomadura cremea subsp. cremea (DSM 43676, ATCC 33577, INA 292)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API zym

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014648495

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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