Thermococcus stetteri (DSM 5262, 8315-5, K-3)

Name: Thermococcus stetteri (DSM 5262, 8315-5, K-3)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16864

Type strain

Strain Designation 8315-5, K-3

Culture col. no. DSM 5262 8315-5 K-3 JCM 8559

NCBI tax ID(s) 49900

Special Collections DSMZ JCM

Links

Thermococcus stetteri 8315-5 is an anaerobe, thermophilic prokaryote that was isolated from marine volcanic crater fields of Kraternya cove.

anaerobe thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 47913

DSM 5262,JCM 8559

@ref 20215

Last LPSN update 2025-12-16 09:50:57

Domain Methanobacteriati

Phylum Methanobacteriota

Class Thermococci

Order Thermococcales

Family Thermococcaceae

Genus Thermococcus

Species Thermococcus stetteri

Full scientific name Thermococcus stetteri Miroshnichenko 1990

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1973	MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760)		Medium recipe at MediaDive	Name: MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
1973	TTD-MEDIUM (DSMZ Medium 480b)		Medium recipe at MediaDive	Name: TTD-MEDIUM (DSMZ Medium 480b) Composition: NaCl 25.0 g/l Sulfur 10.0 g/l Casitone 5.0 g/l NaHCO3 1.5 g/l Na2S x 9 H2O 0.5 g/l KH2PO4 0.33 g/l NH4Cl 0.33 g/l CaCl2 x 2 H2O 0.33 g/l MgCl2 x 6 H2O 0.33 g/l KCl 0.33 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l Pyridoxine hydrochloride 0.0001 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Nicotinic acid 5e-05 g/l Riboflavin 5e-05 g/l Thiamine HCl 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l (DL)-alpha-Lipoic acid 5e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
1973	positive	growth	75	thermophilic
67770	positive	growth	75	thermophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
1973	anaerobe
125439	anaerobe	99.6

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Terrestrial	#Volcanic

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
1973	marine volcanic crater fields of Kraternya cove	Northern Kurils, Ushishir archipelago	Russia	RUS	Asia
67770	Marine solfataric fields of the Kraternaya cove	Northern Kurils

Taxonmaps

69479

Global distribution of 16S sequence Z75240 (>99% sequence identity) for Thermococcus from Microbeatlas

Aquatic Samples	25
Soil Samples	2
Animal Samples
Plant Samples
Total Samples	27

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1973	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1787333v1 assembly for Thermococcus stetteri DSM 5262	contig	GCA_017873335	49900.4	2913342618	49900	75.87

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Thermococcus stetteri strain DSM 5262 16S ribosomal RNA gene, partial sequence	AY099186	1442	49900
20218	Thermococcus stetteri strain DSM 5262 16S-23S ribosomal RNA intergenic spacer region, complete sequence	AY099222	182	49900
20218	T.stetteri 16S rRNA and 23S rRNA genes	Z75240	1916	49900

GC content

@ref	GC-content (mol%)	Method
67770	50.2	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Thermococcus stetteri DSM 5262
NCBI: GCA_017873335

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	82.30	no
125439	motility	BacteriaNetⓘ	no	68.00	no
125439	gram_stain	BacteriaNetⓘ	negative	80.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.60	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Purification and Properties of a Highly Thermostable, Sodium Dodecyl Sulfate-Resistant and Stereospecific Proteinase from the Extremely Thermophilic Archaeon Thermococcus stetteri.	Klingeberg M, Galunsky B, Sjoholm C, Kasche V, Antranikian G.	Appl Environ Microbiol	10.1128/aem.61.8.3098-3104.1995	1995
	Keratin Degradation by Fervidobacterium pennavorans, a Novel Thermophilic Anaerobic Species of the Order Thermotogales.	Friedrich AB, Antranikian G.	Appl Environ Microbiol	10.1128/aem.62.8.2875-2882.1996	1996
Phylogeny	PAV1, the first virus-like particle isolated from a hyperthermophilic euryarchaeote, "Pyrococcus abyssi".	Geslin C, Le Romancer M, Erauso G, Gaillard M, Perrot G, Prieur D.	J Bacteriol	10.1128/jb.185.13.3888-3894.2003	2003

References

#1973	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5262
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thermococcus stetteri (DSM 5262, 8315-5, K-3)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_017873335

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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