Thermoanaerobacter pseudethanolicus (DSM 2355, ATCC 33223, 39E)

Name: Thermoanaerobacter pseudethanolicus (DSM 2355, ATCC 33223, 39E)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16848

Type strain

Strain Designation 39E

Culture col. no. DSM 2355 ATCC 33223 39E

NCBI tax ID(s) 340099 496866

Special Collections DSMZ Literature strains

History DSMZ <- J. G. Zeikus, University of Wisconsin, Department of Bacteriology, Madison, Wisconsin, USA; 39E (Clostridium thermohydrosulfuricum)

Links

Thermoanaerobacter pseudethanolicus 39E is an anaerobe, spore-forming, Gram-positive bacterium that produces alcohol and was isolated from thermal spring.

alcohol production spore-forming Gram-positive motile rod-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 47592

DSM 2355,ATCC 33223,KCTC 1676

@ref 20215

Last LPSN update 2026-05-29 08:28:52

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Thermoanaerobacterales

Family Thermoanaerobacteraceae

Genus Thermoanaerobacter

Species Thermoanaerobacter pseudethanolicus

Full scientific name Thermoanaerobacter pseudethanolicus Onyenwoke et al. 2007

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
32158	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
750	THERMOANAEROBACTER BROCKII MEDIUM (DSMZ Medium 144)		Medium recipe at MediaDive	Name: THERMOANAEROBACTER BROCKII MEDIUM (DSMZ Medium 144) Composition: Trypticase peptone 10.0 g/l D-Glucose 5.0 g/l Yeast extract 3.0 g/l K2HPO4 1.5 g/l Na2S x 9 H2O 1.0 g/l NH4Cl 0.9 g/l KH2PO4 0.75 g/l MgCl2 x 6 H2O 0.4 g/l Nitrilotriacetic acid 0.1152 g/l NaCl 0.009 g/l FeSO4 x 7 H2O 0.003 g/l FeCl2 x 4 H2O 0.0018 g/l CoCl2 x 6 H2O 0.00153 g/l MnCl2 x 4 H2O 0.0009 g/l CaCl2 x 2 H2O 0.0009 g/l ZnCl2 0.0009 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.00027 g/l Na2SeO3 x 5 H2O 0.00027 g/l CuCl2 0.00018 g/l H3BO3 9e-05 g/l Na2MoO4 x 2 H2O 9e-05 g/l Pyridoxine hydrochloride 5e-05 g/l p-Aminobenzoic acid 2.5e-05 g/l (DL)-alpha-Lipoic acid 2.5e-05 g/l Riboflavin 2.5e-05 g/l Calcium D-(+)-pantothenate 2.5e-05 g/l Thiamine HCl 2.5e-05 g/l Nicotinic acid 2.5e-05 g/l Biotin 1e-05 g/l Folic acid 1e-05 g/l Vitamin B12 5e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
750	positive	growth	65
32158	positive	optimum	65

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
750	anaerobe
32158	anaerobe
125438	anaerobe	91.36
125439	anaerobe	99.552

Spore formation

32158

Spore formationyes

Compound production

750

Compoundethanol

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
32158	17057 ChEBI	cellobiose	+	carbon source
32158	17234 ChEBI	glucose	+	carbon source
32158	17306 ChEBI	maltose	+	carbon source
32158	17992 ChEBI	sucrose	+	carbon source
32158	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
32158	16136 ChEBI	hydrogen sulfide

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	formaldehyde oxidation	100	3 of 3
66794	L-lactaldehyde degradation	100	3 of 3
66794	lipoate biosynthesis	100	5 of 5
66794	ppGpp biosynthesis	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	sulfopterin metabolism	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	suberin monomers biosynthesis	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	vitamin B1 metabolism	92.31	12 of 13
66794	threonine metabolism	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	isoleucine metabolism	87.5	7 of 8
66794	palmitate biosynthesis	86.36	19 of 22
66794	urea cycle	84.62	11 of 13
66794	peptidoglycan biosynthesis	80	12 of 15
66794	starch degradation	80	8 of 10
66794	cellulose degradation	80	4 of 5
66794	photosynthesis	78.57	11 of 14
66794	valine metabolism	77.78	7 of 9
66794	d-mannose degradation	77.78	7 of 9
66794	glycolysis	76.47	13 of 17
66794	acetate fermentation	75	3 of 4
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	biotin biosynthesis	75	3 of 4
66794	degradation of sugar alcohols	75	12 of 16
66794	pyrimidine metabolism	73.33	33 of 45
66794	pentose phosphate pathway	72.73	8 of 11
66794	NAD metabolism	72.22	13 of 18
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	propanol degradation	71.43	5 of 7
66794	phenylalanine metabolism	69.23	9 of 13
66794	purine metabolism	68.09	64 of 94
66794	glutamate and glutamine metabolism	67.86	19 of 28
66794	acetoin degradation	66.67	2 of 3
66794	molybdenum cofactor biosynthesis	66.67	6 of 9
66794	serine metabolism	66.67	6 of 9
66794	flavin biosynthesis	66.67	10 of 15
66794	octane oxidation	66.67	2 of 3
66794	histidine metabolism	65.52	19 of 29
66794	methionine metabolism	65.38	17 of 26
66794	heme metabolism	64.29	9 of 14
66794	tetrahydrofolate metabolism	64.29	9 of 14
66794	vitamin B6 metabolism	63.64	7 of 11
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	gluconeogenesis	62.5	5 of 8
66794	leucine metabolism	61.54	8 of 13
66794	oxidative phosphorylation	61.54	56 of 91
66794	hydrogen production	60	3 of 5
66794	Entner Doudoroff pathway	60	6 of 10
66794	glycogen metabolism	60	3 of 5
66794	methylglyoxal degradation	60	3 of 5
66794	propionate fermentation	60	6 of 10
66794	arginine metabolism	58.33	14 of 24
66794	glutathione metabolism	57.14	8 of 14
66794	metabolism of disaccharids	54.55	6 of 11
66794	d-xylose degradation	54.55	6 of 11
66794	tryptophan metabolism	52.63	20 of 38
66794	alanine metabolism	51.72	15 of 29
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	kanosamine biosynthesis II	50	1 of 2
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	ethanol fermentation	50	1 of 2
66794	CMP-KDO biosynthesis	50	2 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	50	4 of 8
66794	ketogluconate metabolism	50	4 of 8
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	glycine metabolism	50	5 of 10
66794	glycogen biosynthesis	50	2 of 4
66794	selenocysteine biosynthesis	50	3 of 6
66794	butanoate fermentation	50	2 of 4
66794	adipate degradation	50	1 of 2
66794	cysteine metabolism	50	9 of 18
66794	vitamin B12 metabolism	47.06	16 of 34
66794	isoprenoid biosynthesis	46.15	12 of 26
66794	proline metabolism	45.45	5 of 11
66794	nitrate assimilation	44.44	4 of 9
66794	CO2 fixation in Crenarchaeota	44.44	4 of 9
66794	degradation of hexoses	44.44	8 of 18
66794	citric acid cycle	42.86	6 of 14
66794	degradation of pentoses	42.86	12 of 28
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	non-pathway related	42.11	16 of 38
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	lysine metabolism	40.48	17 of 42
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	4-hydroxyphenylacetate degradation	40	4 of 10
66794	polyamine pathway	39.13	9 of 23
66794	tyrosine metabolism	35.71	5 of 14
66794	lipid metabolism	35.48	11 of 31
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	pantothenate biosynthesis	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	sulfate reduction	30.77	4 of 13
66794	myo-inositol biosynthesis	30	3 of 10
66794	coenzyme M biosynthesis	30	3 of 10
66794	degradation of sugar acids	28	7 of 25
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	ascorbate metabolism	27.27	6 of 22
66794	toluene degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	phenylpropanoid biosynthesis	23.08	3 of 13
66794	lipid A biosynthesis	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
750	thermal spring	Wyoming, Yellowstone Natl. Park, Octopus Spring	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence L09164 (>99% sequence identity) for Thermoanaerobacter from Microbeatlas

Aquatic Samples	592
Soil Samples	200
Animal Samples	1695
Plant Samples	99
Total Samples	2586

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
750	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1908v1 assembly for Thermoanaerobacter pseudethanolicus ATCC 33223 ATCC 33223; 39E	complete	GCA_000019085	340099.6	641522655	340099	99.32

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
32158	Thermoanaerobacter pseudethanolicus ATCC 33223 16S ribosomal RNA gene, complete sequence	L09164	1515		340099

GC content

@ref	GC-content (mol%)
750	34.4±0.3
32158	34.4

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1908v1 assembly for Thermoanaerobacter pseudethanolicus ATCC 33223 ATCC 33223; 39E
BV-BRC: 340099.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.55	no
125439	gram_stain	BacteriaNetⓘ	variable	60.30	no
125439	motility	BacteriaNetⓘ	yes	66.20	no
125439	spore_formation	BacteriaNetⓘ	yes	59.93	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Thermoanaerobacter pseudethanolicus ATCC 33223
NCBI: GCA_000019085.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	65.30	yes
125438	anaerobic	anaerobicⓘ	yes	91.36	yes
125438	aerobic	aerobicⓘ	no	98.61	yes
125438	spore-forming	spore-formingⓘ	yes	73.10	yes
125438	thermophilic	thermophileⓘ	yes	77.28	no
125438	flagellated	motile2+ⓘ	yes	75.68	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Dataset describing the amino acid catabolism of Thermoanaerobacter pseudethanolicus.	Orlygsson J, Scully SM.	Data Brief	10.1016/j.dib.2023.110017	2024
	Dataset describing the influence of culture conditions on the bioreduction of organic acids to alcohols by Thermoanaerobacter pseudethanolicus.	Orlygsson J, Scully SM.	Data Brief	10.1016/j.dib.2023.109962	2024
	Fermentation of Mannitol Extracts From Brown Macro Algae by Thermophilic Clostridia.	Chades T, Scully SM, Ingvadottir EM, Orlygsson J.	Front Microbiol	10.3389/fmicb.2018.01931	2018
	Influence of Culture Conditions on the Bioreduction of Organic Acids to Alcohols by Thermoanaerobacter pseudoethanolicus.	Scully SM, Brown AE, Mueller-Hilger Y, Ross AB, Orlygsson J.	Microorganisms	10.3390/microorganisms9010162	2021
Pathogenicity	Identification of Lung and Blood Microbiota Implicated in COVID-19 Prognosis.	Dereschuk K, Apostol L, Ranjan I, Chakladar J, Li WT, Rajasekaran M, Chang EY, Ongkeko WM.	Cells	10.3390/cells10061452	2021
	Gut microbiota and COVID-19: A systematic review.	SeyedAlinaghi S, Afzalian A, Pashaei Z, Varshochi S, Karimi A, Mojdeganlou H, Mojdeganlou P, Razi A, Ghanadinezhad F, Shojaei A, Amiri A, Dashti M, Ghasemzadeh A, Dadras O, Mehraeen E, Afsahi AM.	Health Sci Rep	10.1002/hsr2.1080	2023
Enzymology	Ethanol tolerance in engineered strains of Clostridium thermocellum.	Olson DG, Maloney MI, Lanahan AA, Cervenka ND, Xia Y, Pech-Canul A, Hon S, Tian L, Ziegler SJ, Bomble YJ, Lynd LR.	Biotechnol Biofuels Bioprod	10.1186/s13068-023-02379-z	2023
	Chemoenzymatic Synthesis of Tenofovir.	Zdun B, Reiter T, Kroutil W, Borowiecki P.	J Org Chem	10.1021/acs.joc.3c01005	2023
Enzymology	Expression of a heat-stable NADPH-dependent alcohol dehydrogenase from Thermoanaerobacter pseudethanolicus 39E in Clostridium thermocellum 1313 results in increased hydroxymethylfurfural resistance.	Kim SK, Groom J, Chung D, Elkins J, Westpheling J.	Biotechnol Biofuels	10.1186/s13068-017-0750-z	2017
	Mechanism of furfural toxicity and metabolic strategies to engineer tolerance in microbial strains.	Jilani SB, Olson DG.	Microb Cell Fact	10.1186/s12934-023-02223-x	2023
Phylogeny	Rare Events of Intragenus and Intraspecies Horizontal Transfer of the 16S rRNA Gene.	Tian RM, Cai L, Zhang WP, Cao HL, Qian PY.	Genome Biol Evol	10.1093/gbe/evv143	2015
Metabolism	[Cellulose degradation and ethanol production of different Clostridium strain].	Fang ZG, Ouyang ZY.	Huan Jing Ke Xue		2010
Enzymology	Biochemical characterization of engineered amylopullulanase from Thermoanaerobacter ethanolicus 39E-implicating the non-necessity of its 100 C-terminal amino acid residues.	Lin HY, Chuang HH, Lin FP.	Extremophiles	10.1007/s00792-008-0168-4	2008
Metabolism	[Enhanced role of the co-culture of thermophilic anaerobic bacteria on cellulosic ethanol].	Fang ZG.	Huan Jing Ke Xue		2010
Enzymology	Expression of a heat-stable NADPH-dependent alcohol dehydrogenase in Caldicellulosiruptor bescii results in furan aldehyde detoxification.	Chung D, Verbeke TJ, Cross KL, Westpheling J, Elkins JG.	Biotechnol Biofuels	10.1186/s13068-015-0287-y	2015
	Draft Genome Sequence of the Thermophile Thermus filiformis ATCC 43280, Producer of Carotenoid-(Di)glucoside-Branched Fatty Acid (Di)esters and Source of Hyperthermostable Enzymes of Biotechnological Interest.	Mandelli F, Oliveira Ramires B, Couger MB, Paixao DA, Camilo CM, Polikarpov I, Prade R, Riano-Pachon DM, Squina FM.	Genome Announc	10.1128/genomea.00475-15	2015
	Increased Susceptibility of Rice Following Insertion of Amylopullulanase Gene, to Brown Spot Caused by Bipolaris oryzae	Ting MY, Shih HD, Lin CY.	Journal of phytopathology.	10.1111/j.1439-0434.2008.01407.x	2008
Enzymology	Cellulosic ethanol production via consolidated bioprocessing at 75 °C by engineered Caldicellulosiruptor bescii.	Chung D, Cha M, Snyder EN, Elkins JG, Guss AM, Westpheling J.	Biotechnol Biofuels	10.1186/s13068-015-0346-4	2015
	Identifying promoters for gene expression in Clostridium thermocellum.	Olson DG, Maloney M, Lanahan AA, Hon S, Hauser LJ, Lynd LR.	Metab Eng Commun	10.1016/j.meteno.2015.03.002	2015
	Evidence of horizontal gene transfer by transposase gene analyses in Fervidobacterium species.	Cuecas A, Kanoksilapatham W, Gonzalez JM.	PLoS One	10.1371/journal.pone.0173961	2017
Enzymology	A comparative multidimensional LC-MS proteomic analysis reveals mechanisms for furan aldehyde detoxification in Thermoanaerobacter pseudethanolicus 39E.	Clarkson SM, Hamilton-Brehm SD, Giannone RJ, Engle NL, Tschaplinski TJ, Hettich RL, Elkins JG.	Biotechnol Biofuels	10.1186/s13068-014-0165-z	2014
Metabolism	Genomic Analysis of Calderihabitans maritimus KKC1, a Thermophilic, Hydrogenogenic, Carboxydotrophic Bacterium Isolated from Marine Sediment.	Omae K, Yoneda Y, Fukuyama Y, Yoshida T, Sako Y.	Appl Environ Microbiol	10.1128/aem.00832-17	2017
Genetics	Expansion segments in bacterial and archaeal 5S ribosomal RNAs.	Stepanov VG, Fox GE.	RNA	10.1261/rna.077123.120	2021
Metabolism	Mining for novel cyclomaltodextrin glucanotransferases unravels the carbohydrate metabolism pathway via cyclodextrins in Thermoanaerobacterales.	Centeno-Leija S, Espinosa-Barrera L, Velazquez-Cruz B, Cardenas-Conejo Y, Virgen-Ortiz R, Valencia-Cruz G, Saenz RA, Marin-Tovar Y, Gomez-Manzo S, Hernandez-Ochoa B, Rocha-Ramirez LM, Zatarain-Palacios R, Osuna-Castro JA, Lopez-Munguia A, Serrano-Posada H.	Sci Rep	10.1038/s41598-021-04569-x	2022
	Complete genome sequence of the chromate-reducing bacterium Thermoanaerobacter thermohydrosulfuricus strain BSB-33.	Bhattacharya P, Barnebey A, Zemla M, Goodwin L, Auer M, Yannone SM.	Stand Genomic Sci	10.1186/s40793-015-0028-7	2015
	Tolerance and metabolic response of Pseudomonas taiwanensis VLB120 towards biomass hydrolysate-derived inhibitors.	Wordofa GG, Kristensen M.	Biotechnol Biofuels	10.1186/s13068-018-1192-y	2018
Metabolism	A Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase mutant derivative highly active and stereoselective on phenylacetone and benzylacetone.	Ziegelmann-Fjeld KI, Musa MM, Phillips RS, Zeikus JG, Vieille C.	Protein Eng Des Sel	10.1093/protein/gzl052	2007
	Functional study of C-terminal domain of the thermoacidophilic raw starch-hydrolyzing alpha-amylase Gt-amy.	Zeng J, Guo J, Tu Y, Yuan L.	Food Sci Biotechnol	10.1007/s10068-019-00673-x	2020
	Expression of a bi-functional and thermostable amylopullulanase in transgenic rice seeds leads to autohydrolysis and altered composition of starch	Chiang CM, Yeh FS, Huang LF, Tseng TH, Chung MC, Wang CS, Lur HS, Shaw JF, Yu SM.	Mol Breed	10.1007/s11032-004-3919-7	2005
	Natural competence in Thermoanaerobacter and Thermoanaerobacterium species.	Shaw AJ, Hogsett DA, Lynd LR.	Appl Environ Microbiol	10.1128/aem.00402-10	2010
Metabolism	Correlation of genomic and physiological traits of thermoanaerobacter species with biofuel yields.	Hemme CL, Fields MW, He Q, Deng Y, Lin L, Tu Q, Mouttaki H, Zhou A, Feng X, Zuo Z, Ramsay BD, He Z, Wu L, Van Nostrand J, Xu J, Tang YJ, Wiegel J, Phelps TJ, Zhou J.	Appl Environ Microbiol	10.1128/aem.05677-11	2011
	Engineering redox-balanced ethanol production in the cellulolytic and extremely thermophilic bacterium, Caldicellulosiruptor bescii.	Williams-Rhaesa AM, Rubinstein GM, Scott IM, Lipscomb GL, Poole Ii FL, Kelly RM, Adams MWW.	Metab Eng Commun	10.1016/j.mec.2018.e00073	2018
Enzymology	Cloning, expression, and characterization of thermostable region of amylopullulanase gene from Thermoanaerobacter ethanolicus 39E.	Lin FP, Leu KL.	Appl Biochem Biotechnol	10.1385/abab:97:1:33	2002
	Structural insights into recognition of c-di-AMP by the ydaO riboswitch.	Gao A, Serganov A.	Nat Chem Biol	10.1038/nchembio.1607	2014
	Effect of thermal and chemical denaturants on Thermoanaerobacter ethanolicus secondary-alcohol dehydrogenase stability and activity.	Burdette DS, Tchernajencko V, Zeikus JG.	Enzyme Microb Technol	10.1016/s0141-0229(00)00192-7	2000
Metabolism	Ethanol production by the hyperthermophilic archaeon Pyrococcus furiosus by expression of bacterial bifunctional alcohol dehydrogenases.	Keller MW, Lipscomb GL, Nguyen DM, Crowley AT, Schut GJ, Scott I, Kelly RM, Adams MWW.	Microb Biotechnol	10.1111/1751-7915.12486	2017
Genetics	Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725.	Dam P, Kataeva I, Yang SJ, Zhou F, Yin Y, Chou W, Poole FL, Westpheling J, Hettich R, Giannone R, Lewis DL, Kelly R, Gilbert HJ, Henrissat B, Xu Y, Adams MW, Adams MW.	Nucleic Acids Res	10.1093/nar/gkq1281	2011
Phylogeny	Intragenomic heterogeneity of 16S rRNA genes causes overestimation of prokaryotic diversity.	Sun DL, Jiang X, Wu QL, Zhou NY.	Appl Environ Microbiol	10.1128/aem.01282-13	2013
Enzymology	[Cloning and expression of the gene for thermostable beta-galactosidase from Thermoanaerobacter ethanolicus in Escherichia coli: purification and properties of the product].	Fokina NA, Velikodvorskaia GA.	Mol Gen Mikrobiol Virusol		1997
	A markerless gene deletion and integration system for Thermoanaerobacter ethanolicus.	Shao X, Zhou J, Olson DG, Lynd LR.	Biotechnol Biofuels	10.1186/s13068-016-0514-1	2016
Metabolism	Physiological, metabolic and biotechnological features of extremely thermophilic microorganisms.	Counts JA, Zeldes BM, Lee LL, Straub CT, Adams MWW, Kelly RM.	Wiley Interdiscip Rev Syst Biol Med	10.1002/wsbm.1377	2017
Metabolism	Physiological roles of pyruvate ferredoxin oxidoreductase and pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum JW/SL-YS485.	Zhou J, Olson DG, Lanahan AA, Tian L, Murphy SJ, Lo J, Lynd LR.	Biotechnol Biofuels	10.1186/s13068-015-0304-1	2015
Genetics	Genome Stability in Engineered Strains of the Extremely Thermophilic Lignocellulose-Degrading Bacterium Caldicellulosiruptor bescii.	Williams-Rhaesa AM, Poole FL, Dinsmore JT, Lipscomb GL, Rubinstein GM, Scott IM, Conway JM, Lee LL, Khatibi PA, Kelly RM, Adams MWW.	Appl Environ Microbiol	10.1128/aem.00444-17	2017
Metabolism	Linking genome content to biofuel production yields: a meta-analysis of major catabolic pathways among select H2 and ethanol-producing bacteria.	Carere CR, Rydzak T, Verbeke TJ, Cicek N, Levin DB, Sparling R.	BMC Microbiol	10.1186/1471-2180-12-295	2012
Enzymology	Analysis of the catalytic center of cyclomaltodextrinase from Thermoanaerobacter ethanolicus 39E.	Podkovyrov SM, Burdette D, Zeikus JG.	FEBS Lett	10.1016/0014-5793(93)81288-b	1993
	Thermoanaerobacterium thermosaccharolyticum beta-glucosidase: a glucose-tolerant enzyme with high specific activity for cellobiose.	Pei J, Pang Q, Zhao L, Fan S, Shi H.	Biotechnol Biofuels	10.1186/1754-6834-5-31	2012
	Sequencing of the amylopullulanase (apu) gene of Thermoanaerobacter ethanolicus 39E, and identification of the active site by site-directed mutagenesis.	Mathupala SP, Lowe SE, Podkovyrov SM, Zeikus JG.	J Biol Chem	10.1016/s0021-9258(19)85426-1	1993
	A new family of very long chain alpha,omega-dicarboxylic acids is a major structural fatty acyl component of the membrane lipids of Thermoanaerobacter ethanolicus 39E.	Jung S, Zeikus JG, Hollingsworth RI.	J Lipid Res	10.1016/s0022-2275(20)40101-4	1994
Enzymology	Cloning, sequencing and biochemical characterization of xylose isomerase from Thermoanaerobacterium saccharolyticum strain B6A-RI.	Lee YE, Ramesh MV, Zeikus JG.	J Gen Microbiol	10.1099/00221287-139-6-1227	1993
	Gene conservation among endospore-forming bacteria reveals additional sporulation genes in Bacillus subtilis.	Traag BA, Pugliese A, Eisen JA, Losick R.	J Bacteriol	10.1128/jb.01778-12	2013
Metabolism	Identification of the uridine 5'-diphosphoglucose (UDP-Glc) binding subunit of cellulose synthase in Acetobacter xylinum using the photoaffinity probe 5-azido-UDP-Glc.	Lin FC, Brown RM, Drake RR, Haley BE.	J Biol Chem	10.1016/s0021-9258(19)34039-6	1990
Genetics	A genomic signature and the identification of new sporulation genes.	Abecasis AB, Serrano M, Alves R, Quintais L, Pereira-Leal JB, Henriques AO.	J Bacteriol	10.1128/jb.02110-12	2013
	Are algal genes in nonphotosynthetic protists evidence of historical plastid endosymbioses?	Stiller JW, Huang J, Ding Q, Tian J, Goodwillie C.	BMC Genomics	10.1186/1471-2164-10-484	2009
Metabolism	Role of alcohols in growth, lipid composition, and membrane fluidity of yeasts, bacteria, and archaea.	Huffer S, Clark ME, Ning JC, Blanch HW, Clark DS.	Appl Environ Microbiol	10.1128/aem.00694-11	2011
Metabolism	A high molecular-mass Anoxybacillus sp. SK3-4 amylopullulanase: characterization and its relationship in carbohydrate utilization.	Kahar UM, Chan KG, Salleh MM, Hii SM, Goh KM.	Int J Mol Sci	10.3390/ijms140611302	2013
Genetics	Protein languages differ depending on microorganism lifestyle.	Grzymski JJ, Marsh AG.	PLoS One	10.1371/journal.pone.0096910	2014
Metabolism	The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is necessary for ethanol production in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum.	Lo J, Zheng T, Hon S, Olson DG, Lynd LR.	J Bacteriol	10.1128/jb.02450-14	2015
Metabolism	Thermoanaerobacter thermohydrosulfuricus WC1 shows protein complement stability during fermentation of key lignocellulose-derived substrates.	Verbeke TJ, Spicer V, Krokhin OV, Zhang X, Schellenberg JJ, Fristensky B, Wilkins JA, Levin DB, Sparling R.	Appl Environ Microbiol	10.1128/aem.03555-13	2014
Metabolism	Identification of a proton-chloride antiporter (EriC) by Himar1 transposon mutagenesis in Lactobacillus reuteri and its role in histamine production.	Hemarajata P, Spinler JK, Balderas MA, Versalovic J.	Antonie Van Leeuwenhoek	10.1007/s10482-014-0113-8	2014
Metabolism	Genomic evaluation of Thermoanaerobacter spp. for the construction of designer co-cultures to improve lignocellulosic biofuel production.	Verbeke TJ, Zhang X, Henrissat B, Spicer V, Rydzak T, Krokhin OV, Fristensky B, Levin DB, Sparling R.	PLoS One	10.1371/journal.pone.0059362	2013
Enzymology	Characterization of thermostable cyclodextrinase from Clostridium thermohydrosulfuricum 39E.	Saha BC, Zeikus JG.	Appl Environ Microbiol	10.1128/aem.56.9.2941-2943.1990	1990
Genetics	A comprehensive benchmarking study of protocols and sequencing platforms for 16S rRNA community profiling.	D'Amore R, Ijaz UZ, Schirmer M, Kenny JG, Gregory R, Darby AC, Shakya M, Podar M, Quince C, Hall N.	BMC Genomics	10.1186/s12864-015-2194-9	2016
Metabolism	Lactobacillus reuteri-specific immunoregulatory gene rsiR modulates histamine production and immunomodulation by Lactobacillus reuteri.	Hemarajata P, Gao C, Pflughoeft KJ, Thomas CM, Saulnier DM, Spinler JK, Versalovic J.	J Bacteriol	10.1128/jb.00261-13	2013
Phylogeny	Enzyme diversity of the cellulolytic system produced by Clostridium cellulolyticum explored by two-dimensional analysis: identification of seven genes encoding new dockerin-containing proteins.	Blouzard JC, Bourgeois C, de Philip P, Valette O, Belaich A, Tardif C, Belaich JP, Pages S.	J Bacteriol	10.1128/jb.00917-06	2007
Metabolism	Characterization of ApuB, an extracellular type II amylopullulanase from Bifidobacterium breve UCC2003.	O'Connell Motherway M, Fitzgerald GF, Neirynck S, Ryan S, Steidler L, van Sinderen D.	Appl Environ Microbiol	10.1128/aem.01169-08	2008
Metabolism	Identification of surprisingly diverse type IV pili, across a broad range of gram-positive bacteria.	Imam S, Chen Z, Roos DS, Pohlschroder M.	PLoS One	10.1371/journal.pone.0028919	2011
Metabolism	The Thermoanaerobacter glycobiome reveals mechanisms of pentose and hexose co-utilization in bacteria.	Lin L, Song H, Tu Q, Qin Y, Zhou A, Liu W, He Z, Zhou J, Xu J.	PLoS Genet	10.1371/journal.pgen.1002318	2011
	Circadian rhythms in the thermophilic cyanobacterium Thermosynechococcus elongatus: compensation of period length over a wide temperature range.	Onai K, Morishita M, Itoh S, Okamoto K, Ishiura M.	J Bacteriol	10.1128/jb.186.15.4972-4977.2004	2004
	Annotation of Protein Domains Reveals Remarkable Conservation in the Functional Make up of Proteomes Across Superkingdoms.	Nasir A, Naeem A, Khan MJ, Nicora HD, Caetano-Anolles G.	Genes (Basel)	10.3390/genes2040869	2011
Metabolism	Screening for and identification of starch-, amylopectin-, and pullulan-degrading activities in bifidobacterial strains.	Ryan SM, Fitzgerald GF, van Sinderen D.	Appl Environ Microbiol	10.1128/aem.00257-06	2006
Phylogeny	Bioinformatic characterization of the 4-Toluene Sulfonate Uptake Permease (TSUP) family of transmembrane proteins.	Shlykov MA, Zheng WH, Chen JS, Saier MH.	Biochim Biophys Acta	10.1016/j.bbamem.2011.12.005	2012
Metabolism	Isolation and characterization of metal-reducing thermoanaerobacter strains from deep subsurface environments of the Piceance Basin, Colorado.	Roh Y, Liu SV, Li G, Huang H, Phelps TJ, Zhou J.	Appl Environ Microbiol	10.1128/aem.68.12.6013-6020.2002	2002
	Ultrastructure and extreme heat resistance of spores from thermophilic Clostridium species.	Hyun HH, Zeikus JG, Longin R, Millet J, Ryter A.	J Bacteriol	10.1128/jb.156.3.1332-1337.1983	1983
	A new thermoactive pullulanase from Desulfurococcus mucosus: cloning, sequencing, purification, and characterization of the recombinant enzyme after expression in Bacillus subtilis.	Duffner F, Bertoldo C, Andersen JT, Wagner K, Antranikian G.	J Bacteriol	10.1128/jb.182.22.6331-6338.2000	2000
Enzymology	Biophysical and mutagenic analysis of Thermoanaerobacter ethanolicus secondary-alcohol dehydrogenase activity and specificity.	Burdette DS, Secundo F, Phillips RS, Dong J, Scott RA, Zeikus JG.	Biochem J	10.1042/bj3260717	1997
Enzymology	Cloning and expression of the gene encoding the Thermoanaerobacter ethanolicus 39E secondary-alcohol dehydrogenase and biochemical characterization of the enzyme.	Burdette DS, Vieille C, Zeikus JG.	Biochem J	10.1042/bj3160115	1996
Enzymology	Structure of the gene encoding cyclomaltodextrinase from Clostridium thermohydrosulfuricum 39E and characterization of the enzyme purified from Escherichia coli.	Podkovyrov SM, Zeikus JG.	J Bacteriol	10.1128/jb.174.16.5400-5405.1992	1992
Genetics	The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum).	Pierce E, Xie G, Barabote RD, Saunders E, Han CS, Detter JC, Richardson P, Brettin TS, Das A, Ljungdahl LG, Ragsdale SW.	Environ Microbiol	10.1111/j.1462-2920.2008.01679.x	2008
Enzymology	Purification of acetaldehyde dehydrogenase and alcohol dehydrogenases from Thermoanaerobacter ethanolicus 39E and characterization of the secondary-alcohol dehydrogenase (2 degrees Adh) as a bifunctional alcohol dehydrogenase--acetyl-CoA reductive thioesterase.	Burdette D, Zeikus JG.	Biochem J	10.1042/bj3020163	1994
Metabolism	Homolactic Acid Fermentation by the Genetically Engineered Thermophilic Homoacetogen Moorella thermoacetica ATCC 39073.	Iwasaki Y, Kita A, Yoshida K, Tajima T, Yano S, Shou T, Saito M, Kato J, Murakami K, Nakashimada Y	Appl Environ Microbiol	10.1128/AEM.00247-17	2017
Metabolism	Characterization of Electrical Current-Generation Capabilities from Thermophilic Bacterium Thermoanaerobacter pseudethanolicus Using Xylose, Glucose, Cellobiose, or Acetate with Fixed Anode Potentials.	Lusk BG, Khan QF, Parameswaran P, Hameed A, Ali N, Rittmann BE, Torres CI	Environ Sci Technol	10.1021/acs.est.5b04036	2015
Metabolism	Development of genetic transformation and heterologous expression system in carboxydotrophic thermophilic acetogen Moorella thermoacetica.	Kita A, Iwasaki Y, Sakai S, Okuto S, Takaoka K, Suzuki T, Yano S, Sawayama S, Tajima T, Kato J, Nishio N, Murakami K, Nakashimada Y	J Biosci Bioeng	10.1016/j.jbiosc.2012.10.013	2012
Phylogeny	Thermoanaerobacter pseudethanolicus sp. nov., a thermophilic heterotrophic anaerobe from Yellowstone National Park.	Onyenwoke RU, Kevbrin VV, Lysenko AM, Wiegel J	Int J Syst Evol Microbiol	10.1099/ijs.0.65051-0	2007

References

#750	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2355
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28401	IJSEM 2191 2007 ( DOI 10.1099/ijs.0.65051-0 , PubMed 17911280 )
#32158	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28401
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thermoanaerobacter pseudethanolicus (DSM 2355, ATCC 33223, 39E)

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