Name: Thermoanaerobacter pseudethanolicus 39E
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 16848

Type strain:

Species: Thermoanaerobacter pseudethanolicus

Strain Designation: 39E

Culture col. no.: DSM 2355, ATCC 33223

Strain history: <- J.G. Zeikus, 39E (Clostridium thermohydrosulfuricum)

NCBI tax ID(s): 340099 (strain), 496866 (species)

SI-ID 47592

DSM 2355, ATCC 33223, KCTC 1676

Section

Thermoanaerobacter pseudethanolicus 39E is an anaerobe, spore-forming, thermophilic bacterium that produces alcohol and was isolated from thermal spring.

alcohol production
Gram-positive
motile
rod-shaped
spore-forming
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Thermoanaerobacterales*
	Family	*Thermoanaerobacteraceae*
	Genus	*Thermoanaerobacter*
	Species	**Thermoanaerobacter pseudethanolicus**
	Full Scientific Name (LPSN)	Thermoanaerobacter pseudethanolicus Onyenwoke et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #32158]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %99.747

[Ref.: #32158]	Cell shape	rod-shaped

[Ref.: #32158]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %93.912

Information on culture and growth conditions Culture and growth conditions

[Ref.: #750]

Culture medium

THERMOANAEROBACTER BROCKII MEDIUM (DSMZ Medium 144)

[Ref.: #750]

Culture medium growth

[Ref.: #750]

Culture medium link

Medium recipe at MediaDive

[Ref.: #750]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #750]			growth	65 °C
[Ref.: #32158]			optimum	65 °C

[Ref.: #750]	Temperature range	thermophilic
[Ref.: #32158]	Temperature range	thermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %100

Information on physiology and metabolism Physiology and metabolism

[Ref.: #750]	Oxygen tolerance	anaerobe
[Ref.: #32158]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #32158]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.448

[Ref.: #750]

Name of produced compound

ethanol

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32158]	cellobiose ChEBI	+	carbon source
[Ref.: #32158]	glucose ChEBI	+	carbon source
[Ref.: #32158]	maltose ChEBI	+	carbon source
[Ref.: #32158]	sucrose ChEBI	+	carbon source
[Ref.: #32158]	xylose ChEBI	+	carbon source

	Metabolite production	Metabolite	Production
[Ref.: #32158]		hydrogen sulfide ChEBI	yes

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	lipoate biosynthesis	100	5 of 5
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	sulfopterin metabolism	100	4 of 4
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	vitamin B1 metabolism	92.31	12 of 13
[Ref.: #66794]	threonine metabolism	90	9 of 10
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	isoleucine metabolism	87.5	7 of 8
[Ref.: #66794]	palmitate biosynthesis	86.36	19 of 22
[Ref.: #66794]	urea cycle	84.62	11 of 13
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	starch degradation	80	8 of 10
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	valine metabolism	77.78	7 of 9
[Ref.: #66794]	d-mannose degradation	77.78	7 of 9
[Ref.: #66794]	glycolysis	76.47	13 of 17
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	C4 and CAM-carbon fixation	75	6 of 8
[Ref.: #66794]	biotin biosynthesis	75	3 of 4
[Ref.: #66794]	degradation of sugar alcohols	75	12 of 16
[Ref.: #66794]	pyrimidine metabolism	73.33	33 of 45
[Ref.: #66794]	pentose phosphate pathway	72.73	8 of 11
[Ref.: #66794]	NAD metabolism	72.22	13 of 18
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	propanol degradation	71.43	5 of 7
[Ref.: #66794]	phenylalanine metabolism	69.23	9 of 13
[Ref.: #66794]	purine metabolism	68.09	64 of 94
[Ref.: #66794]	glutamate and glutamine metabolism	67.86	19 of 28
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	molybdenum cofactor biosynthesis	66.67	6 of 9
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	flavin biosynthesis	66.67	10 of 15
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	histidine metabolism	65.52	19 of 29
[Ref.: #66794]	methionine metabolism	65.38	17 of 26
[Ref.: #66794]	heme metabolism	64.29	9 of 14
[Ref.: #66794]	tetrahydrofolate metabolism	64.29	9 of 14
[Ref.: #66794]	vitamin B6 metabolism	63.64	7 of 11
[Ref.: #66794]	dTDPLrhamnose biosynthesis	62.5	5 of 8
[Ref.: #66794]	gluconeogenesis	62.5	5 of 8
[Ref.: #66794]	leucine metabolism	61.54	8 of 13
[Ref.: #66794]	oxidative phosphorylation	61.54	56 of 91
[Ref.: #66794]	hydrogen production	60	3 of 5
[Ref.: #66794]	Entner Doudoroff pathway	60	6 of 10
[Ref.: #66794]	glycogen metabolism	60	3 of 5
[Ref.: #66794]	methylglyoxal degradation	60	3 of 5
[Ref.: #66794]	propionate fermentation	60	6 of 10
[Ref.: #66794]	arginine metabolism	58.33	14 of 24
[Ref.: #66794]	glutathione metabolism	57.14	8 of 14
[Ref.: #66794]	metabolism of disaccharids	54.55	6 of 11
[Ref.: #66794]	d-xylose degradation	54.55	6 of 11
[Ref.: #66794]	tryptophan metabolism	52.63	20 of 38
[Ref.: #66794]	alanine metabolism	51.72	15 of 29
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	kanosamine biosynthesis II	50	1 of 2
[Ref.: #66794]	glycolate and glyoxylate degradation	50	3 of 6
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	CMP-KDO biosynthesis	50	2 of 4
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	50	4 of 8
[Ref.: #66794]	ketogluconate metabolism	50	4 of 8
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	glycogen biosynthesis	50	2 of 4
[Ref.: #66794]	selenocysteine biosynthesis	50	3 of 6
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	adipate degradation	50	1 of 2
[Ref.: #66794]	cysteine metabolism	50	9 of 18
[Ref.: #66794]	vitamin B12 metabolism	47.06	16 of 34
[Ref.: #66794]	isoprenoid biosynthesis	46.15	12 of 26
[Ref.: #66794]	proline metabolism	45.45	5 of 11
[Ref.: #66794]	nitrate assimilation	44.44	4 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	44.44	4 of 9
[Ref.: #66794]	degradation of hexoses	44.44	8 of 18
[Ref.: #66794]	citric acid cycle	42.86	6 of 14
[Ref.: #66794]	degradation of pentoses	42.86	12 of 28
[Ref.: #66794]	reductive acetyl coenzyme A pathway	42.86	3 of 7
[Ref.: #66794]	non-pathway related	42.11	16 of 38
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
[Ref.: #66794]	lysine metabolism	40.48	17 of 42
[Ref.: #66794]	metabolism of amino sugars and derivatives	40	2 of 5
[Ref.: #66794]	4-hydroxyphenylacetate degradation	40	4 of 10
[Ref.: #66794]	polyamine pathway	39.13	9 of 23
[Ref.: #66794]	tyrosine metabolism	35.71	5 of 14
[Ref.: #66794]	lipid metabolism	35.48	11 of 31
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	pantothenate biosynthesis	33.33	2 of 6
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	sulfate reduction	30.77	4 of 13
[Ref.: #66794]	myo-inositol biosynthesis	30	3 of 10
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	degradation of sugar acids	28	7 of 25
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	ascorbate metabolism	27.27	6 of 22
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	phenylpropanoid biosynthesis	23.08	3 of 13
[Ref.: #66794]	lipid A biosynthesis	22.22	2 of 9
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #750]	Sample type/isolated from	thermal spring
[Ref.: #750]	Geographic location (country and/or sea, region)	Wyoming, Yellowstone Natl. Park, Octopus Spring
[Ref.: #750]	Country	USA
[Ref.: #750]	Country ISO 3 Code	USA
[Ref.: #750]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence L09164 (>99% sequence identity) for Thermoanaerobacter from Microbeatlas

Aquatic Samples	592
Soil Samples	200
Animal Samples	1695
Plant Samples	99
Total Samples	2586

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #750]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #32158]	Thermoanaerobacter pseudethanolicus ATCC 33223 16S ribosomal RNA gene, complete sequence	L09164	1515	GenBank	340099 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thermoanaerobacter pseudethanolicus ATCC 33223 ATCC 33223; 39E	GCA_000019085	complete	GenBank	340099 tax ID	*
[Ref.: #66792]	Thermoanaerobacter pseudethanolicus ATCC 33223	340099.6	complete	PATRIC	340099 tax ID	*
[Ref.: #66792]	Thermoanaerobacter pseudethanolicus 39E, ATCC 33223	641522655	complete	JGI IMG/M	340099 tax ID	*

[Ref.: #750]	GC-content	34.4±0.3 mol%
[Ref.: #32158]	GC-content	34.4 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Thermoanaerobacter pseudethanolicus ATCC 33223 NCBI: GCA_000019085.1
[Ref.: #69481]	spore-forming	yes	85	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thermoanaerobacter pseudethanolicus ATCC 33223 NCBI: GCA_000019085.1
[Ref.: #69480]	motile	yes	86.337	no
[Ref.: #69480]	flagellated	no	89.136	no
[Ref.: #69480]	gram-positive	no	61.08	yes
[Ref.: #69480]	anaerobic	yes	99.23	yes
[Ref.: #69480]	aerobic	no	98.297	no
[Ref.: #69480]	halophile	no	92.913	no
[Ref.: #69480]	spore-forming	yes	92.958	yes
[Ref.: #69480]	thermophile	yes	100	yes
[Ref.: #69480]	glucose-util	yes	94.165	no
[Ref.: #69480]	glucose-ferment	yes	59.541	no

Availability in culture collections External links

[Ref.: #750]

Culture collection no.

DSM 2355, ATCC 33223

[Ref.: #85857]

SI-ID 47592

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Homolactic Acid Fermentation by the Genetically Engineered Thermophilic Homoacetogen Moorella thermoacetica ATCC 39073.	Iwasaki Y, Kita A, Yoshida K, Tajima T, Yano S, Shou T, Saito M, Kato J, Murakami K, Nakashimada Y	Appl Environ Microbiol	10.1128/AEM.00247-17	2017	*
Metabolism	Characterization of Electrical Current-Generation Capabilities from Thermophilic Bacterium Thermoanaerobacter pseudethanolicus Using Xylose, Glucose, Cellobiose, or Acetate with Fixed Anode Potentials.	Lusk BG, Khan QF, Parameswaran P, Hameed A, Ali N, Rittmann BE, Torres CI	Environ Sci Technol	10.1021/acs.est.5b04036	2015	*
Metabolism	Development of genetic transformation and heterologous expression system in carboxydotrophic thermophilic acetogen Moorella thermoacetica.	Kita A, Iwasaki Y, Sakai S, Okuto S, Takaoka K, Suzuki T, Yano S, Sawayama S, Tajima T, Kato J, Nishio N, Murakami K, Nakashimada Y	J Biosci Bioeng	10.1016/j.jbiosc.2012.10.013	2012	*
Phylogeny	Thermoanaerobacter pseudethanolicus sp. nov., a thermophilic heterotrophic anaerobe from Yellowstone National Park.	Onyenwoke RU, Kevbrin VV, Lysenko AM, Wiegel J	Int J Syst Evol Microbiol	10.1099/ijs.0.65051-0	2007	*

References

#750	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2355

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32158

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28401 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#85857

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID47592.1 )

#28401

IJSEM 2191 2007 ( DOI 10.1099/ijs.0.65051-0 , PubMed 17911280 )

* These data were automatically processed and therefore are not curated

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Thermoanaerobacter pseudethanolicus ATCC 33223

Thermoanaerobacter pseudethanolicus ATCC 33223

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