Thermoanaerobacter ethanolicus (DSM 2246, ATCC 31550, VKM B-1835)

Name: Thermoanaerobacter ethanolicus (DSM 2246, ATCC 31550, VKM B-1835)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16832

Type strain

Strain Designation JW200

Culture col. no. DSM 2246 ATCC 31550 VKM B-1835 JW200

NCBI tax ID(s) 1757

Special Collections DSMZ

History DSMZ <- J. Wiegel, Universität Göttingen, Institut für Mikrobiologie, Germany; JW200 <- J. Wiegel {1978}

Links

Thermoanaerobacter ethanolicus JW200 is an anaerobe bacterium that produces alcohol and was isolated from mud, alkaline hot spring.

alcohol production anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 7714

LMG 12893,ATCC 31550,DSM 2246,VKM B-1835

@ref 20215

Last LPSN update 2026-05-29 08:28:52

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Thermoanaerobacterales

Family Thermoanaerobacteraceae

Genus Thermoanaerobacter

Species Thermoanaerobacter ethanolicus

Full scientific name Thermoanaerobacter ethanolicus Wiegel and Ljungdahl 1982

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
705	CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640)		Medium recipe at MediaDive	Name: CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640) Composition: Trypticase peptone 2.0 g/l K2HPO4 1.5 g/l Cellobiose 1.0 g/l Yeast extract 1.0 g/l NH4Cl 0.9 g/l NaCl 0.9 g/l L-Cysteine HCl x H2O 0.75 g/l KH2PO4 0.75 g/l MgCl2 x 6 H2O 0.4 g/l HCl 0.0025 g/l FeCl3 x 6 H2O 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
705	positive	growth	65

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
705	anaerobe
125438	anaerobe	95.275
125439	anaerobe	99.124

Compound production

@ref	Compound
705	amylase
705	ethanol
705	pullulanase
705	alcohol dehydrogenase

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Mud (Sludge)
#Environmental	#Aquatic	#Thermal spring
#Condition	#Alkaline	-
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
705	mud, alkaline hot spring	Wyoming, Yellowstone Natl. Park	USA	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
705	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM372231v1 assembly for Thermoanaerobacter ethanolicus JW 200	complete	GCA_003722315	509192.5	2841155221	509192	97.25

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
705	Thermoanaerobacter ethanolicus JW 200 16S ribosomal RNA gene, partial sequence	L09162	1740		1757

GC content

705

GC-content (mol%)31.9

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM372231v1 assembly for Thermoanaerobacter ethanolicus JW 200
NCBI: GCA_003722315

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.12	no
125439	gram_stain	BacteriaNetⓘ	variable	59.07	no
125439	motility	BacteriaNetⓘ	yes	63.79	no
125439	spore_formation	BacteriaNetⓘ	yes	59.06	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Thermoanaerobacter ethanolicus JW 200
PATRIC: 509192.5

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	54.52	no
125438	anaerobic	anaerobicⓘ	yes	95.28	yes
125438	spore-forming	spore-formingⓘ	yes	75.44	no
125438	aerobic	aerobicⓘ	no	95.74	yes
125438	thermophilic	thermophileⓘ	yes	78.01	yes
125438	flagellated	motile2+ⓘ	yes	75.12	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome Editing of the Anaerobic Thermophile Thermoanaerobacter ethanolicus Using Thermostable Cas9.	Le Y, Fu Y, Sun J.	Appl Environ Microbiol	10.1128/aem.01773-20	2020
	Natural competence in Thermoanaerobacter and Thermoanaerobacterium species.	Shaw AJ, Hogsett DA, Lynd LR.	Appl Environ Microbiol	10.1128/aem.00402-10	2010
Metabolism	Ethanol production by the hyperthermophilic archaeon Pyrococcus furiosus by expression of bacterial bifunctional alcohol dehydrogenases.	Keller MW, Lipscomb GL, Nguyen DM, Crowley AT, Schut GJ, Scott I, Kelly RM, Adams MWW.	Microb Biotechnol	10.1111/1751-7915.12486	2017
	Fermentation of Mannitol Extracts From Brown Macro Algae by Thermophilic Clostridia.	Chades T, Scully SM, Ingvadottir EM, Orlygsson J.	Front Microbiol	10.3389/fmicb.2018.01931	2018
	Differences in Xylan Degradation by Various Noncellulolytic Thermophilic Anaerobes and Clostridium thermocellum.	Wiegel J, Mothershed CP, Puls J.	Appl Environ Microbiol	10.1128/aem.49.3.656-659.1985	1985
	Keratin Degradation by Fervidobacterium pennavorans, a Novel Thermophilic Anaerobic Species of the Order Thermotogales.	Friedrich AB, Antranikian G.	Appl Environ Microbiol	10.1128/aem.62.8.2875-2882.1996	1996
Enzymology	Clostridium thermocellum JW20 (ATCC 31549) is a coculture with Thermoanaerobacter ethanolicus.	Erbeznik M, Jones CR, Dawson KA, Strobel HJ.	Appl Environ Microbiol	10.1128/aem.63.7.2949-2951.1997	1997
Metabolism	Strain selection in carbon-limited chemostats affects reproducibility of Thermoanaerobacter ethanolicus fermentations.	Lacis LS, Lawford HG.	Appl Environ Microbiol	10.1128/aem.58.2.761-764.1992	1992
Enzymology	Purification and characterization of a thermostable beta-xylosidase from Thermoanaerobacter ethanolicus.	Shao W, Wiegel J.	J Bacteriol	10.1128/jb.174.18.5848-5853.1992	1992
	Thermoanaerobacter ethanolicus in a comparison of the growth efficiencies of thermophilic and mesophilic anaerobes.	Lacis LS, Lawford HG.	J Bacteriol	10.1128/jb.163.3.1275-1278.1985	1985
	Thermoanaerobacter ethanolicus Growth and Product Yield from Elevated Levels of Xylose or Glucose in Continuous Cultures.	Lacis LS, Lawford HG.	Appl Environ Microbiol	10.1128/aem.57.2.579-585.1991	1991
Phylogeny	Phylogenetic analysis of anaerobic thermophilic bacteria: aid for their reclassification.	Rainey FA, Ward NL, Morgan HW, Toalster R, Stackebrandt E.	J Bacteriol	10.1128/jb.175.15.4772-4779.1993	1993
	Single-step ethanol production from lignocellulose using novel extremely thermophilic bacteria.	Svetlitchnyi VA, Kensch O, Falkenhan DA, Korseska SG, Lippert N, Prinz M, Sassi J, Schickor A, Curvers S.	Biotechnol Biofuels	10.1186/1754-6834-6-31	2013
Metabolism	Electrotransformation of Thermoanaerobacter ethanolicus JW200.	Peng H, Fu B, Mao Z, Shao W	Biotechnol Lett	10.1007/s10529-006-9184-6	2006
	A markerless gene deletion and integration system for Thermoanaerobacter ethanolicus.	Shao X, Zhou J, Olson DG, Lynd LR	Biotechnol Biofuels	10.1186/s13068-016-0514-1	2016
Metabolism	Amino Acid Metabolism of Thermoanaerobacter Strain AK90: The Role of Electron-Scavenging Systems in End Product Formation.	Scully SM, Orlygsson J	J Amino Acids	10.1155/2015/410492	2015
Metabolism	Binding mode of the oxidized alpha-anomer of NAD+ to RSP, a Rex-family repressor.	Zheng Y, Ko TP, Yang Y, Shao W, Guo RT	Biochem Biophys Res Commun	10.1016/j.bbrc.2014.12.049	2014
Metabolism	Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes.	Zheng Y, Ko TP, Sun H, Huang CH, Pei J, Qiu R, Wang AH, Wiegel J, Shao W, Guo RT	J Struct Biol	10.1016/j.jsb.2014.11.001	2014
Metabolism	Enhanced catalytic efficiency in quercetin-4'-glucoside hydrolysis of Thermotoga maritima beta-glucosidase A by site-directed mutagenesis.	Sun H, Xue Y, Lin Y	J Agric Food Chem	10.1021/jf501932v	2014
	Teth137, a Conserved Factor of Unknown Function from Thermoanaerobacter ethanolicus JW200, Represses the Transcription of the adhE Gene In Vitro.	Jing Q, Wang J, Wu G	Indian J Microbiol	10.1007/s12088-012-0339-y	2012
Metabolism	[Characterization of an acidotolerant, thermophilic Thermoanaerobacter sp. xyl-d with a high xylose conversion].	Zhang W, Ma S, Deng Y, Zhang H	Wei Sheng Wu Xue Bao		2011
Enzymology	Comparison of three thermostable beta-glucosidases for application in the hydrolysis of soybean isoflavone glycosides.	Song X, Xue Y, Wang Q, Wu X	J Agric Food Chem	10.1021/jf1046915	2011
Enzymology	Cloning and analysis of the xylAB operon and characterization of xylose isomerase from Thermoanaerobacter ethanolicus.	Fan L, Zhang Y, Qu W, Wang J, Shao W	Biotechnol Lett	10.1007/s10529-010-0463-x	2010
Enzymology	Molecular genetic characterization of the thermostable L-lactate dehydrogenase gene (ldhL) of Thermoanaerobacter ethanolicus JW200 and biochemical characterization of the enzyme.	Zhou Q, Shao WL	Biochemistry (Mosc)	10.1134/s0006297910040188	2010
Enzymology	The aldehyde/alcohol dehydrogenase (AdhE) in relation to the ethanol formation in Thermoanaerobacter ethanolicus JW200.	Peng H, Wu G, Shao W	Anaerobe	10.1016/j.anaerobe.2007.09.004	2007
Metabolism	Effect of nutrient limitation on product formation during continuous fermentation of xylose with Thermoanaerobacter ethanolicus JW200 Fe(7).	Hild HM, Stuckey DC, Leak DJ	Appl Microbiol Biotechnol	10.1007/s00253-002-1175-5	2002
Enzymology	Cloning, sequencing and expression in Escherichia coli of the primary alcohol dehydrogenase gene from Thermoanaerobacter ethanolicus JW200.	Holt PJ, Williams RE, Jordan KN, Lowe CR, Bruce NC	FEMS Microbiol Lett	10.1111/j.1574-6968.2000.tb09262.x	2000
Enzymology	Cloning, sequencing, and characterization of the bifunctional xylosidase-arabinosidase from the anaerobic thermophile thermoanaerobacter ethanolicus.	Mai V, Wiegel J, Lorenz WW	Gene	10.1016/s0378-1119(00)00106-2	2000
	Induction and Regeneration of Autoplasts from Clostridium thermohydrosulfuricum JW102 and Thermoanaerobacter ethanolicus JW200.	Peteranderl R, Canganella F, Holzenburg A, Wiegel J	Appl Environ Microbiol	10.1128/aem.59.10.3498-3501.1993	1993
Enzymology	Purification and Properties of Primary and Secondary Alcohol Dehydrogenases from Thermoanaerobacter ethanolicus.	Bryant FO, Wiegel J, Ljungdahl LG	Appl Environ Microbiol	10.1128/aem.54.2.460-465.1988	1988

References

#705	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2246
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thermoanaerobacter ethanolicus (DSM 2246, ATCC 31550, VKM B-1835)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Compound production

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003722315

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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