Name: Thermoanaerobacter ethanolicus JW200
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 16832

Type strain:

Species: Thermoanaerobacter ethanolicus

Strain Designation: JW200

Culture col. no.: DSM 2246, ATCC 31550, VKM B-1835

Strain history: <- J. Wiegel, Universität Göttingen, Institut für Mikrobiologie, Germany; JW200 <- J. Wiegel {1978}

NCBI tax ID(s): 1757 (species)

SI-ID 7714

LMG 12893, ATCC 31550, DSM 2246, VKM B-1835

Section

Thermoanaerobacter ethanolicus JW200 is an anaerobe, spore-forming, thermophilic bacterium that produces alcohol and was isolated from mud, alkaline hot spring.

alcohol production
motile
spore-forming
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Thermoanaerobacterales*
	Family	*Thermoanaerobacteraceae*
	Genus	*Thermoanaerobacter*
	Species	**Thermoanaerobacter ethanolicus**
	Full Scientific Name (LPSN)	Thermoanaerobacter ethanolicus Wiegel and Ljungdahl 1982

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	yes Confidence in %94.844

Information on culture and growth conditions Culture and growth conditions

[Ref.: #705]

Culture medium

CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640)

[Ref.: #705]

Culture medium growth

[Ref.: #705]

Culture medium link

Medium recipe at MediaDive

[Ref.: #705]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #705]			growth	65 °C

[Ref.: #705]	Temperature range	thermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %99.999

Information on physiology and metabolism Physiology and metabolism

[Ref.: #705]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #69481]	Ability of spore formation	yes Confidence in %98
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.999

[Ref.: #705]	Name of produced compound	amylase
[Ref.: #705]	Name of produced compound	ethanol
[Ref.: #705]	Name of produced compound	pullulanase
[Ref.: #705]	Name of produced compound	alcohol dehydrogenase

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #705]	Sample type/isolated from	mud, alkaline hot spring
[Ref.: #705]	Geographic location (country and/or sea, region)	Wyoming, Yellowstone Natl. Park
[Ref.: #705]	Country	USA
[Ref.: #705]	Country ISO 3 Code	USA
[Ref.: #705]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Mud (Sludge)
#Environmental	#Aquatic	#Thermal spring
#Condition	#Alkaline	-
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #705]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #705]	Thermoanaerobacter ethanolicus JW 200 16S ribosomal RNA gene, partial sequence	L09162	1740	ENA	1757 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thermoanaerobacter ethanolicus JW 200	509192.5	complete	PATRIC	509192 tax ID	*

[Ref.: #705]

GC-content

31.9 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Thermoanaerobacter ethanolicus JW 200 PATRIC: 509192.5
[Ref.: #69481]	spore-forming	yes	98	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thermoanaerobacter ethanolicus JW 200 PATRIC: 509192.5
[Ref.: #69480]	gram-positive	no	73.035	no
[Ref.: #69480]	anaerobic	yes	99.333	yes
[Ref.: #69480]	halophile	no	96.714	no
[Ref.: #69480]	spore-forming	yes	93.786	no
[Ref.: #69480]	glucose-util	yes	93.024	no
[Ref.: #69480]	aerobic	no	98.496	no
[Ref.: #69480]	thermophile	yes	100	yes
[Ref.: #69480]	motile	yes	82.254	no
[Ref.: #69480]	flagellated	no	85.775	no
[Ref.: #69480]	glucose-ferment	yes	54.191	no

Availability in culture collections External links

[Ref.: #705]

Culture collection no.

DSM 2246, ATCC 31550, VKM B-1835

[Ref.: #85843]

SI-ID 7714

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Electrotransformation of Thermoanaerobacter ethanolicus JW200.	Peng H, Fu B, Mao Z, Shao W	Biotechnol Lett	10.1007/s10529-006-9184-6	2006	*
Metabolism	Amino Acid Metabolism of Thermoanaerobacter Strain AK90: The Role of Electron-Scavenging Systems in End Product Formation.	Scully SM, Orlygsson J	J Amino Acids	10.1155/2015/410492	2015	*
Metabolism	Binding mode of the oxidized alpha-anomer of NAD+ to RSP, a Rex-family repressor.	Zheng Y, Ko TP, Yang Y, Shao W, Guo RT	Biochem Biophys Res Commun	10.1016/j.bbrc.2014.12.049	2014	*
Metabolism	Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes.	Zheng Y, Ko TP, Sun H, Huang CH, Pei J, Qiu R, Wang AH, Wiegel J, Shao W, Guo RT	J Struct Biol	10.1016/j.jsb.2014.11.001	2014	*
Metabolism	Enhanced catalytic efficiency in quercetin-4'-glucoside hydrolysis of Thermotoga maritima beta-glucosidase A by site-directed mutagenesis.	Sun H, Xue Y, Lin Y	J Agric Food Chem	10.1021/jf501932v	2014	*
Metabolism	[Characterization of an acidotolerant, thermophilic Thermoanaerobacter sp. xyl-d with a high xylose conversion].	Zhang W, Ma S, Deng Y, Zhang H	Wei Sheng Wu Xue Bao		2011	*
Enzymology	Comparison of three thermostable beta-glucosidases for application in the hydrolysis of soybean isoflavone glycosides.	Song X, Xue Y, Wang Q, Wu X	J Agric Food Chem	10.1021/jf1046915	2011	*
Enzymology	Cloning and analysis of the xylAB operon and characterization of xylose isomerase from Thermoanaerobacter ethanolicus.	Fan L, Zhang Y, Qu W, Wang J, Shao W	Biotechnol Lett	10.1007/s10529-010-0463-x	2010	*
Enzymology	Molecular genetic characterization of the thermostable L-lactate dehydrogenase gene (ldhL) of Thermoanaerobacter ethanolicus JW200 and biochemical characterization of the enzyme.	Zhou Q, Shao WL	Biochemistry (Mosc)	10.1134/s0006297910040188	2010	*
Enzymology	The aldehyde/alcohol dehydrogenase (AdhE) in relation to the ethanol formation in Thermoanaerobacter ethanolicus JW200.	Peng H, Wu G, Shao W	Anaerobe	10.1016/j.anaerobe.2007.09.004	2007	*
Metabolism	Effect of nutrient limitation on product formation during continuous fermentation of xylose with Thermoanaerobacter ethanolicus JW200 Fe(7).	Hild HM, Stuckey DC, Leak DJ	Appl Microbiol Biotechnol	10.1007/s00253-002-1175-5	2002	*
Enzymology	Cloning, sequencing and expression in Escherichia coli of the primary alcohol dehydrogenase gene from Thermoanaerobacter ethanolicus JW200.	Holt PJ, Williams RE, Jordan KN, Lowe CR, Bruce NC	FEMS Microbiol Lett	10.1111/j.1574-6968.2000.tb09262.x	2000	*
Enzymology	Cloning, sequencing, and characterization of the bifunctional xylosidase-arabinosidase from the anaerobic thermophile thermoanaerobacter ethanolicus.	Mai V, Wiegel J, Lorenz WW	Gene	10.1016/s0378-1119(00)00106-2	2000	*
Enzymology	Purification and Properties of Primary and Secondary Alcohol Dehydrogenases from Thermoanaerobacter ethanolicus.	Bryant FO, Wiegel J, Ljungdahl LG	Appl Environ Microbiol	10.1128/aem.54.2.460-465.1988	1988	*
	A markerless gene deletion and integration system for Thermoanaerobacter ethanolicus.	Shao X, Zhou J, Olson DG, Lynd LR	Biotechnol Biofuels	10.1186/s13068-016-0514-1	2016	*
	Teth137, a Conserved Factor of Unknown Function from Thermoanaerobacter ethanolicus JW200, Represses the Transcription of the adhE Gene In Vitro.	Jing Q, Wang J, Wu G	Indian J Microbiol	10.1007/s12088-012-0339-y	2012	*
	Induction and Regeneration of Autoplasts from Clostridium thermohydrosulfuricum JW102 and Thermoanaerobacter ethanolicus JW200.	Peteranderl R, Canganella F, Holzenburg A, Wiegel J	Appl Environ Microbiol	10.1128/aem.59.10.3498-3501.1993	1993	*

References

#705	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2246

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#85843

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID7714.1 )

* These data were automatically processed and therefore are not curated

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Thermoanaerobacter ethanolicus JW 200

Thermoanaerobacter ethanolicus JW 200

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