Bifidobacterium adolescentis (DSM 20086, ATCC 15705, CCUG 24603)

Name: Bifidobacterium adolescentis (DSM 20086, ATCC 15705, CCUG 24603)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1683

Type strain

Strain Designation E 298 b (Variant c)

Culture col. no. DSM 20086 ATCC 15705 CCUG 24603 E 298 b (Variant c) JCM 7046 3 more

NCBI tax ID(s) 1680

Special Collections DSMZ CCUG JCM CIP

History <- G. Reuter, E 298 b (Variant c) T. Mitsuoka IV-46 <-- G. Reuter E298b (Variant c).

Links

Bifidobacterium adolescentis E 298 b is an anaerobe bacterium that was isolated from intestine of adult.

anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 13593

LMG 10733,ATCC 15705,CCUG 24603,DSM 20086,JCM 7046,NCFB 2230,NCDO 2230,CCUG 45215,CCRC 14608,NCIMB 702230,CIP 64.60,KCTC 3267,BCRC 14608,VTT E-991436

@ref 20215

Last LPSN update 2026-02-09 11:18:09

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Bifidobacteriales

Family Bifidobacteriaceae

Genus Bifidobacterium

Species Bifidobacterium adolescentis

Full scientific name Bifidobacterium adolescentis Reuter 1963 (Approved Lists 1980)

Synonyms (2)

Bifidobacterium stercoris
Bifidobacterium faecale

BacDive ID	Other strains from **Bifidobacterium adolescentis** (15)	Type strain
1682	B. adolescentis E194a (Variant a), E194a, DSM 20083, ATCC ... (type strain)
1684	B. adolescentis RU 424 (Mannitol fermenter), DSM 20087, JCM ...
1748	B. adolescentis Eg1, DSM 24849, JCM 15918, KCTC 5756, BCRC ...
137496	B. adolescentis E319a, CIP 64.61, ATCC 15706, NCDO 2231, ...
138910	B. adolescentis CIP 64.58, ATCC 15704, NCDO 2229, CCUG 45214, ...
151924	B. adolescentis CCUG 45215, CIP 64.60
152740	B. adolescentis CCUG 47519
153440	B. adolescentis CCUG 49594 A
157629	B. adolescentis ORG 4, ORG4, DSM 28529
157630	B. adolescentis ORG 8, ORG8, DSM 28530
157631	B. adolescentis f-BAD, DSM 107523
157632	B. adolescentis H1_9, DSM 108262
157633	B. adolescentis H4_11, DSM 108263
162521	B. adolescentis JCM 19480
165468	B. adolescentis JCM 7045, LMG 18898

Morphology

Cell morphology

@ref	Motility	Confidence
125438		93

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
8512	BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)		Medium recipe at MediaDive	Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
8512	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

8512

Oxygen toleranceanaerobe

Spore formation

@ref	Spore formation	Confidence
125439		98.5
125438		90.96

Murein

@ref	Murein short key	Type
8512	A11.32	A4alpha L-Lys(L-Orn)-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68381	40585 ChEBI	alpha-cyclodextrin	-	builds acid from	from API rID32STR
68381	29016 ChEBI	arginine	-	hydrolysis	from API rID32STR
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
68381	18333 ChEBI	D-arabitol	-	builds acid from	from API rID32STR
68381	16899 ChEBI	D-mannitol	-	builds acid from	from API rID32STR
68380	16024 ChEBI	D-mannose	-	fermentation	from API rID32A
68381	16988 ChEBI	D-ribose	-	builds acid from	from API rID32STR
68381	16443 ChEBI	D-tagatose	-	builds acid from	from API rID32STR
68381	28087 ChEBI	glycogen	-	builds acid from	from API rID32STR
68381	606565 ChEBI	hippurate	-	hydrolysis	from API rID32STR
68381	30849 ChEBI	L-arabinose	-	builds acid from	from API rID32STR
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
68381	17716 ChEBI	lactose	+	builds acid from	from API rID32STR
68381	17306 ChEBI	maltose	-	builds acid from	from API rID32STR
68381	6731 ChEBI	melezitose	-	builds acid from	from API rID32STR
68381	28053 ChEBI	melibiose	+	builds acid from	from API rID32STR
68381	320055 ChEBI	methyl beta-D-glucopyranoside	-	builds acid from	from API rID32STR
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
68381	27941 ChEBI	pullulan	-	builds acid from	from API rID32STR
68381	16634 ChEBI	raffinose	+	builds acid from	from API rID32STR
68380	16634 ChEBI	raffinose	+	fermentation	from API rID32A
68381	30911 ChEBI	sorbitol	-	builds acid from	from API rID32STR
68381	17992 ChEBI	sucrose	+	builds acid from	from API rID32STR
68381	27082 ChEBI	trehalose	-	builds acid from	from API rID32STR
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
68381	16199 ChEBI	urea	-	hydrolysis	from API rID32STR
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68381	15688 ChEBI	acetoin		from API rID32STR
68380	35581 ChEBI	indole		from API rID32A

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68381	15688 ChEBI	acetoin	-		from API rID32STR
68380	35581 ChEBI	indole		-	from API rID32A

Enzymes

@ref	Value	Activity	Ec
68380	alanine arylamidase	+	3.4.11.2	from API rID32A
68381	Alanyl-Phenylalanyl-Proline arylamidase	+		from API rID32STR
68380	alkaline phosphatase	-	3.1.3.1	from API rID32A
68381	alkaline phosphatase	-	3.1.3.1	from API rID32STR
68380	alpha-arabinosidase	+	3.2.1.55	from API rID32A
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68381	alpha-galactosidase	+	3.2.1.22	from API rID32STR
68380	alpha-galactosidase	+	3.2.1.22	from API rID32A
68380	alpha-glucosidase	+	3.2.1.20	from API rID32A
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
68381	arginine dihydrolase	-	3.5.3.6	from API rID32STR
68381	beta-galactosidase	+	3.2.1.23	from API rID32STR
68380	beta-galactosidase	+	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68380	beta-glucosidase	+	3.2.1.21	from API rID32A
68381	beta-glucosidase	+	3.2.1.21	from API rID32STR
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
68381	beta-glucuronidase	-	3.2.1.31	from API rID32STR
68381	beta-mannosidase	-	3.2.1.25	from API rID32STR
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	-		from API rID32A
68381	glycyl tryptophan arylamidase	-		from API rID32STR
68380	histidine arylamidase	+		from API rID32A
68380	L-arginine arylamidase	+		from API rID32A
68380	leucine arylamidase	+	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
68381	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32STR
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
68380	phenylalanine arylamidase	+		from API rID32A
68380	proline-arylamidase	+	3.4.11.5	from API rID32A
68381	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32STR
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
68380	tyrosine arylamidase	+		from API rID32A
68381	urease	-	3.5.1.5	from API rID32STR
68380	urease	-	3.5.1.5	from API rID32A

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
47992	-	-	+	+	-	+	+	+	-	-	-	+	-	-	-	-	-	+	+	-	+	+	-	+	+	-	+	-	-

API rID32STR

@ref	ADH (Arg)	beta GLU	beta GAR	beta GUR	alpha GAL	PAL	RIB	MAN	SOR	LAC	TRE	RAF	SAC	LARA	DARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	HIP	GLYG	PUL	MAL	MEL	MLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	TAG	beta MAN	URE
47992	-	+	+	-	+	-	-	-	-	+	-	+	+	-	-	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Host Body-Site	#Gastrointestinal tract

Isolation

@ref	Sample type	Sampling date
8512	intestine of adult
47992	Intestine of adult	1989-05-02
67770	Intestine of adult

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
8512	yes, in single cases	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	BadoLMG10733v1 assembly for Bifidobacterium adolescentis LMG 10733	contig	GCA_002108135	1680.44	8025235266	1680	77.56
124043	ASM2528914v1 assembly for Bifidobacterium adolescentis E298b (Variant c)	scaffold	GCA_025289145	1680.2224		1680	75.49

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
124043	Bifidobacterium adolescentis gene for 16S rRNA, partial sequence, strain: JCM 7046.	AB116273	479		1680

GC content

@ref	GC-content (mol%)	Method
8512	61.8
67770	61.8	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Bifidobacterium adolescentis LMG 10733
NCBI: GCA_002108135

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	98.50	no
125439	motility	BacteriaNetⓘ	yes	50.30	no
125439	gram_stain	BacteriaNetⓘ	negative	67.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	82.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Bifidobacterium adolescentis strain LMG 10733
PATRIC: 1680.44

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.78	no
125438	anaerobic	anaerobicⓘ	yes	87.56	yes
125438	spore-forming	spore-formingⓘ	no	90.96	no
125438	aerobic	aerobicⓘ	no	92.89	yes
125438	thermophilic	thermophileⓘ	no	93.60	yes
125438	flagellated	motile2+ⓘ	no	93.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete genome sequence of Bifidobacterium adolescentis P2P3, a human gut bacterium possessing strong resistant starch-degrading activity.	Jung DH, Chung WH, Seo DH, Kim YJ, Nam YD, Park CS.	3 Biotech	10.1007/s13205-019-2019-7	2020
	Resistant starch utilization by Bifidobacterium, the beneficial human gut bacteria.	Jung DH, Park CS.	Food Sci Biotechnol	10.1007/s10068-023-01253-w	2023
	Four Types of TiO₂ Reduced the Growth of Selected Lactic Acid Bacteria Strains.	Baranowska-Wojcik E, Gustaw K, Szwajgier D, Oleszczuk P, Pawlikowska-Pawlega B, Pawelec J, Kapral-Piotrowska J.	Foods	10.3390/foods10050939	2021
Phylogeny	Comparison of various molecular methods for rapid differentiation of intestinal bifidobacteria at the species, subspecies and strain level.	Jarocki P, Podlesny M, Komon-Janczara E, Kucharska J, Glibowska A, Targonski Z.	BMC Microbiol	10.1186/s12866-016-0779-3	2016
Metabolism	Resistant starch, microbiome, and precision modulation.	Dobranowski PA, Stintzi A.	Gut Microbes	10.1080/19490976.2021.1926842	2021
Enzymology	Genetic diversity of bile salt hydrolases among human intestinal bifidobacteria.	Jarocki P, Targonski Z.	Curr Microbiol	10.1007/s00284-013-0362-1	2013
Metabolism	Two routes of metabolic cross-feeding between Bifidobacterium adolescentis and butyrate-producing anaerobes from the human gut.	Belenguer A, Duncan SH, Calder AG, Holtrop G, Louis P, Lobley GE, Flint HJ.	Appl Environ Microbiol	10.1128/aem.72.5.3593-3599.2006	2006
Phylogeny	Identification of commensal bacterial strains that modulate Yersinia enterocolitica and dextran sodium sulfate-induced inflammatory responses: implications for the development of probiotics.	Frick JS, Fink K, Kahl F, Niemiec MJ, Quitadamo M, Schenk K, Autenrieth IB.	Infect Immun	10.1128/iai.00119-07	2007
	Bifidobacterial species differentially affect expression of cell surface markers and cytokines of dendritic cells harvested from cord blood.	Young SL, Simon MA, Baird MA, Tannock GW, Bibiloni R, Spencely K, Lane JM, Fitzharris P, Crane J, Town I, Addo-Yobo E, Murray CS, Woodcock A.	Clin Diagn Lab Immunol	10.1128/cdli.11.4.686-690.2004	2004
Enzymology	Quantification of human fecal bifidobacterium species by use of quantitative real-time PCR analysis targeting the groEL gene.	Junick J, Blaut M.	Appl Environ Microbiol	10.1128/aem.07749-11	2012
Phylogeny	Bifidobacteria in feces and environmental waters.	Lamendella R, Santo Domingo JW, Kelty C, Oerther DB.	Appl Environ Microbiol	10.1128/aem.01221-07	2008
Metabolism	Adhesion of bifidobacteria to granular starch and its implications in probiotic technologies.	Crittenden R, Laitila A, Forssell P, Matto J, Saarela M, Mattila-Sandholm T, Myllarinen P.	Appl Environ Microbiol	10.1128/aem.67.8.3469-3475.2001	2001
	Evidence for the Worldwide Distribution of a Bile Salt Hydrolase Gene in Enterococcus faecium Through Horizontal Gene Transfer.	Kusada H, Tamaki H.	Int J Mol Sci	10.3390/ijms26020612	2025
	Modulation of Designed Gut Bacterial Communities by Prebiotics and the Impact of Their Metabolites on Intestinal Cells.	Roupar D, Gonzalez A, Martins JT, Goncalves DA, Teixeira JA, Botelho C, Nobre C.	Foods	10.3390/foods12234216	2023
	Bile Salt Hydrolases with Extended Substrate Specificity Confer a High Level of Resistance to Bile Toxicity on Atopobiaceae Bacteria.	Morinaga K, Kusada H, Tamaki H.	Int J Mol Sci	10.3390/ijms231810980	2022
	Plasmacytoid dendritic cells are crucial in Bifidobacterium adolescentis-mediated inhibition of Yersinia enterocolitica infection.	Wittmann A, Autenrieth IB, Frick JS.	PLoS One	10.1371/journal.pone.0071338	2013
Genetics	Single-cell genomics for resolution of conserved bacterial genes and mobile genetic elements of the human intestinal microbiota using flow cytometry.	Lawrence D, Campbell DE, Schriefer LA, Rodgers R, Walker FC, Turkin M, Droit L, Parkes M, Handley SA, Baldridge MT.	Gut Microbes	10.1080/19490976.2022.2029673	2022
Metabolism	Isolation of a bifidogenic peptide from the pepsin hydrolysate of bovine lactoferrin.	Oda H, Wakabayashi H, Yamauchi K, Sato T, Xiao JZ, Abe F, Iwatsuki K.	Appl Environ Microbiol	10.1128/aem.03343-12	2013
Metabolism	Diversity, ecology and intestinal function of bifidobacteria.	Bottacini F, Ventura M, van Sinderen D, O'Connell Motherway M.	Microb Cell Fact	10.1186/1475-2859-13-s1-s4	2014
Enzymology	Quantitative real-time PCR assays to identify and quantify fecal Bifidobacterium species in infants receiving a prebiotic infant formula.	Haarman M, Knol J.	Appl Environ Microbiol	10.1128/aem.71.5.2318-2324.2005	2005
Enzymology	Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis.	Satokari RM, Vaughan EE, Akkermans AD, Saarela M, de Vos WM.	Appl Environ Microbiol	10.1128/aem.67.2.504-513.2001	2001
Phylogeny	Identification of Bifidobacterium strains by rRNA gene restriction patterns.	Mangin I, Bourget N, Bouhnik Y, Bisetti N, Simonet JM, Decaris B.	Appl Environ Microbiol	10.1128/aem.60.5.1451-1458.1994	1994
	Growth inhibition of Streptococcus mutans by cellular extracts of human intestinal lactic acid bacteria.	Ishihara K, Miyakawa H, Hasegawa A, Takazoe I, Kawai Y.	Infect Immun	10.1128/iai.49.3.692-694.1985	1985
Enzymology	Extracellular dextranase activity produced by human oral strains of the genus Bifidobacterium.	Kaster AG, Brown LR.	Infect Immun	10.1128/iai.42.2.716-720.1983	1983
	Agarooligosaccharides as a novel concept in prebiotics: selective inhibition of Ruminococcus gnavus and Fusobacterium nucleatum while preserving Bifidobacteria, Lactobacillales in vitro, and inhibiting Lachnospiraceae in vivo.	Fujii T, Karasawa K, Takahashi H, Shirai I, Funasaka K, Ohno E, Hirooka Y, Tochio T.	Microbiology (Reading)	10.1099/mic.0.001510	2024
Metabolism	In vitro kinetic analysis of fermentation of prebiotic inulin-type fructans by Bifidobacterium species reveals four different phenotypes.	Falony G, Lazidou K, Verschaeren A, Weckx S, Maes D, De Vuyst L.	Appl Environ Microbiol	10.1128/aem.01488-08	2009
Metabolism	Ability of bifidobacteria to metabolize chitin-glucan and its impact on the gut microbiota.	Alessandri G, Milani C, Duranti S, Mancabelli L, Ranjanoro T, Modica S, Carnevali L, Statello R, Bottacini F, Turroni F, Ossiprandi MC, Sgoifo A, van Sinderen D, Ventura M.	Sci Rep	10.1038/s41598-019-42257-z	2019
Pathogenicity	Antibodies to Lactobacilli and Bifidobacteria in young children with different propensity to develop islet autoimmunity.	Talja I, Kubo AL, Veijola R, Knip M, Simell O, Ilonen J, Vaha-Makila M, Sepp E, Mikelsaar M, Utt M, Uibo R	J Immunol Res	10.1155/2014/325938	2014
Phylogeny	Intra-species diversity between seven Bifidobacterium adolescentis strains identified by genome-wide tiling array analysis.	Yasui K, Tabata M, Yamada S, Abe T, Ikemura T, Osawa R, Suzuki T	Biosci Biotechnol Biochem	10.1271/bbb.80843	2009

References

#8512	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20086
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#47992	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 24603
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68380	Automatically annotated from API rID32A .
#68381	Automatically annotated from API rID32STR .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Bifidobacterium adolescentis (DSM 20086, ATCC 15705, CCUG 24603)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Murein

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API rID32A

API rID32STR

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002108135

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media