Thermoanaerobacter kivui (DSM 2030, ATCC 33488, LKT-1)

Name: Thermoanaerobacter kivui (DSM 2030, ATCC 33488, LKT-1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16823

Type strain

Strain Designation LKT-1

Culture col. no. DSM 2030 ATCC 33488 LKT-1

NCBI tax ID(s) 2325

Special Collections DSMZ

History <- J. A. Leigh and R. S. Wolfe, University of Illinois, Departments of Dairy Science and Microbiology, Urbana, USA; LKT-1

Links

Thermoanaerobacter kivui LKT-1 is an anaerobe prokaryote that was isolated from mud.

anaerobe genome sequence 16S sequence

Name and taxonomic classification

SI-ID 44960

ATCC 33488,DSM 2030

@ref 20215

Last LPSN update 2026-02-09 11:22:03

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Thermoanaerobacterales

Family Thermoanaerobacteraceae

Genus Thermoanaerobacter

Species Thermoanaerobacter kivui

Full scientific name Thermoanaerobacter kivui (Leigh and Wolfe 1983) Collins et al. 1994

Synonyms (1)

Acetogenium kivui

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
676	METHANOBACTERIUM MEDIUM (DSMZ Medium 119)		Medium recipe at MediaDive	Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water
676	THERMOANAEROBACTER KIVUI MEDIUM (DSMZ Medium 171)		Medium recipe at MediaDive	Name: THERMOANAEROBACTER KIVUI MEDIUM (DSMZ Medium 171) Composition: Na2HPO4 x 12 H2O 5.99214 g/l NaH2PO4 x H2O 4.42043 g/l L-Cysteine HCl x H2O 0.491159 g/l Na2S x 9 H2O 0.491159 g/l NH4Cl 0.304519 g/l KH2PO4 0.21611 g/l K2HPO4 0.21611 g/l (NH4)2SO4 0.21611 g/l MgSO4 x 7 H2O 0.0294695 g/l Nitrilotriacetic acid 0.0147348 g/l NaCl 0.00982318 g/l MnSO4 x H2O 0.00491159 g/l FeSO4 x 7 H2O 0.00196464 g/l CoSO4 x 7 H2O 0.00176817 g/l ZnSO4 x 7 H2O 0.00176817 g/l CaCl2 x 2 H2O 0.000982318 g/l Sodium resazurin 0.000491159 g/l NiCl2 x 6 H2O 0.000294695 g/l AlK(SO4)2 x 12 H2O 0.000196464 g/l Na2MoO4 x 2 H2O 9.82318e-05 g/l H3BO3 9.82318e-05 g/l CuSO4 x 5 H2O 9.82318e-05 g/l Na2WO4 x 2 H2O 3.92927e-06 g/l Na2SeO3 x 5 H2O 2.94695e-06 g/l H2SO4 Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
676	positive	growth	60

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
676	anaerobe
125438	anaerobe	91.779
125439	anaerobe	100

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Mud (Sludge)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
676	mud	(formerly Zaire) Lake Kivu	Democratic Republic of the Congo	COD	Africa

Taxonmaps

69479

Global distribution of 16S sequence L09160 (>99% sequence identity) for Thermoanaerobacter kivui subclade from Microbeatlas

Aquatic Samples	55
Soil Samples	10
Animal Samples	11
Plant Samples
Total Samples	76

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
676	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Thermoanaerobacter kivui DSM 2030	complete			2576861811	2325	98.86
66792	ASM76357v1 assembly for Thermoanaerobacter kivui DSM 2030; LKT-1	complete	GCA_000763575	2325.3	2627853919	2325	95.33
124043	TKVG1v2 assembly for Thermoanaerobacter kivui LKT-1 substr. G1	complete	GCA_963971585			2325	87.74

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
676	Thermoanaerobacter kivui strain DSM 2030 16S ribosomal RNA gene, partial sequence	L09160	1662		2325

GC content

676

GC-content (mol%)38.0

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Thermoanaerobacter kivui DSM 2030
PATRIC: 2325.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	70.40	no
125439	motility	BacteriaNetⓘ	yes	74.50	no
125439	gram_stain	BacteriaNetⓘ	variable	80.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	100.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Thermoanaerobacter kivui DSM 2030
NCBI: GCA_000763575.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	59.69	no
125438	anaerobic	anaerobicⓘ	yes	91.78	yes
125438	aerobic	aerobicⓘ	no	96.91	yes
125438	spore-forming	spore-formingⓘ	yes	75.37	no
125438	thermophilic	thermophileⓘ	yes	75.02	no
125438	flagellated	motile2+ⓘ	yes	72.73	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Sequencing of a Thermoanaerobacter kivui isolate from DSMZ stock: major differences with reference assembly.	Hocq R, Thallinger GG, Pflugl S.	Microbiol Resour Announc	10.1128/mra.01250-24	2025
Genetics	Hi-TARGET: a fast, efficient and versatile CRISPR type I-B genome editing tool for the thermophilic acetogen Thermoanaerobacter kivui.	Sitara A, Hocq R, Lu AJ, Pflugl S.	Biotechnol Biofuels Bioprod	10.1186/s13068-025-02647-0	2025
	A megatransposon drives the adaptation of Thermoanaerobacter kivui to carbon monoxide.	Hocq R, Horvath J, Stumptner M, Malevicius M, Thallinger GG, Pflugl S.	Nat Commun	10.1038/s41467-025-59103-8	2025
Enzymology	The energy-converting hydrogenase Ech2 is important for the growth of the thermophilic acetogen Thermoanaerobacter kivui on ferredoxin-dependent substrates.	Baum C, Zeldes B, Poehlein A, Daniel R, Muller V, Basen M.	Microbiol Spectr	10.1128/spectrum.03380-23	2024
	A fluorescent reporter system for anaerobic thermophiles.	Hocq R, Bottone S, Gautier A, Pflugl S.	Front Bioeng Biotechnol	10.3389/fbioe.2023.1226889	2023
Enzymology	Obligate autotrophy at the thermodynamic limit of life in a new acetogenic bacterium.	Frolov EN, Elcheninov AG, Gololobova AV, Toshchakov SV, Novikov AA, Lebedinsky AV, Kublanov IV.	Front Microbiol	10.3389/fmicb.2023.1185739	2023
	DNA uptake from a laboratory environment drives unexpected adaptation of a thermophile to a minor medium component.	Zeldes B, Poehlein A, Jain S, Baum C, Daniel R, Muller V, Basen M.	ISME Commun	10.1038/s43705-022-00211-7	2023
Metabolism	The pyruvate:ferredoxin oxidoreductase of the thermophilic acetogen, Thermoanaerobacter kivui.	Katsyv A, Schoelmerich MC, Basen M, Muller V.	FEBS Open Bio	10.1002/2211-5463.13136	2021
Enzymology	A purified energy-converting hydrogenase from Thermoanaerobacter kivui demonstrates coupled H⁺-translocation and reduction in vitro.	Katsyv A, Muller V.	J Biol Chem	10.1016/j.jbc.2022.102216	2022
Enzymology	The monofunctional CO dehydrogenase CooS is essential for growth of Thermoanaerobacter kivui on carbon monoxide.	Jain S, Katsyv A, Basen M, Muller V.	Extremophiles	10.1007/s00792-021-01251-y	2021
Enzymology	Formate-driven H₂ production by whole cells of Thermoanaerobacter kivui.	Burger Y, Schwarz FM, Muller V.	Biotechnol Biofuels Bioprod	10.1186/s13068-022-02147-5	2022
Enzymology	Electron carriers involved in autotrophic and heterotrophic acetogenesis in the thermophilic bacterium Thermoanaerobacter kivui.	Katsyv A, Jain S, Basen M, Muller V.	Extremophiles	10.1007/s00792-021-01247-8	2021
Metabolism	A Genetic System for the Thermophilic Acetogenic Bacterium Thermoanaerobacter kivui.	Basen M, Geiger I, Henke L, Muller V.	Appl Environ Microbiol	10.1128/aem.02210-17	2018
	Revealing formate production from carbon monoxide in wild type and mutants of Rnf- and Ech-containing acetogens, Acetobacterium woodii and Thermoanaerobacter kivui.	Schwarz FM, Ciurus S, Jain S, Baum C, Wiechmann A, Basen M, Muller V.	Microb Biotechnol	10.1111/1751-7915.13663	2020
Enzymology	Whole-cell biocatalysis for hydrogen storage and syngas conversion to formate using a thermophilic acetogen.	Schwarz FM, Muller V.	Biotechnol Biofuels	10.1186/s13068-020-1670-x	2020
Enzymology	Molecular Basis of the Electron Bifurcation Mechanism in the [FeFe]-Hydrogenase Complex HydABC.	Katsyv A, Kumar A, Saura P, Poverlein MC, Freibert SA, T Stripp S, Jain S, Gamiz-Hernandez AP, Kaila VRI, Muller V, Schuller JM.	J Am Chem Soc	10.1021/jacs.2c11683	2023
Metabolism	Energy conservation by a hydrogenase-dependent chemiosmotic mechanism in an ancient metabolic pathway.	Schoelmerich MC, Muller V.	Proc Natl Acad Sci U S A	10.1073/pnas.1818580116	2019
Enzymology	Energy-converting hydrogenases: the link between H₂ metabolism and energy conservation.	Schoelmerich MC, Muller V.	Cell Mol Life Sci	10.1007/s00018-019-03329-5	2020
Metabolism	Carbon Isotope Fractionation during Catabolism and Anabolism in Acetogenic Bacteria Growing on Different Substrates.	Freude C, Blaser M.	Appl Environ Microbiol	10.1128/aem.03502-15	2016
Enzymology	Hydrogenation of CO₂ at ambient pressure catalyzed by a highly active thermostable biocatalyst.	Schwarz FM, Schuchmann K, Muller V.	Biotechnol Biofuels	10.1186/s13068-018-1236-3	2018
	Energy conservation under extreme energy limitation: the role of cytochromes and quinones in acetogenic bacteria.	Rosenbaum FP, Muller V.	Extremophiles	10.1007/s00792-021-01241-0	2021
Metabolism	Carbon isotope fractionation of 11 acetogenic strains grown on H2 and CO2.	Blaser MB, Dreisbach LK, Conrad R.	Appl Environ Microbiol	10.1128/aem.03203-12	2013
Metabolism	CO Metabolism in the Thermophilic Acetogen Thermoanaerobacter kivui.	Weghoff MC, Muller V.	Appl Environ Microbiol	10.1128/aem.00122-16	2016
Metabolism	A genome-guided analysis of energy conservation in the thermophilic, cytochrome-free acetogenic bacterium Thermoanaerobacter kivui.	Hess V, Poehlein A, Weghoff MC, Daniel R, Muller V.	BMC Genomics	10.1186/1471-2164-15-1139	2014
Metabolism	The Sporomusa type Nfn is a novel type of electron-bifurcating transhydrogenase that links the redox pools in acetogenic bacteria.	Kremp F, Roth J, Muller V.	Sci Rep	10.1038/s41598-020-71038-2	2020
Genetics	Metagenomic analysis of soybean endosphere microbiome to reveal signatures of microbes for health and disease.	Chouhan U, Gamad U, Choudhari JK.	J Genet Eng Biotechnol	10.1186/s43141-023-00535-4	2023
Metabolism	Cysteine: an overlooked energy and carbon source.	Gobbels L, Poehlein A, Dumnitch A, Egelkamp R, Kroger C, Haerdter J, Hackl T, Feld A, Weller H, Daniel R, Streit WR, Schoelmerich MC.	Sci Rep	10.1038/s41598-021-81103-z	2021
	Fermentation of Mannitol Extracts From Brown Macro Algae by Thermophilic Clostridia.	Chades T, Scully SM, Ingvadottir EM, Orlygsson J.	Front Microbiol	10.3389/fmicb.2018.01931	2018
Transcriptome	The Complete Genome Sequence of Clostridium aceticum: a Missing Link between Rnf- and Cytochrome-Containing Autotrophic Acetogens.	Poehlein A, Cebulla M, Ilg MM, Bengelsdorf FR, Schiel-Bengelsdorf B, Whited G, Andreesen JR, Gottschalk G, Daniel R, Durre P.	mBio	10.1128/mbio.01168-15	2015
Genetics	Hitherto unknown [Fe-Fe]-hydrogenase gene diversity in anaerobes and anoxic enrichments from a moderately acidic fen.	Schmidt O, Drake HL, Horn MA.	Appl Environ Microbiol	10.1128/aem.02895-09	2010
Metabolism	Tolerance and metabolic response of acetogenic bacteria toward oxygen.	Karnholz A, Kusel K, Gossner A, Schramm A, Drake HL.	Appl Environ Microbiol	10.1128/aem.68.2.1005-1009.2002	2002
Metabolism	Physiological roles of pyruvate ferredoxin oxidoreductase and pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum JW/SL-YS485.	Zhou J, Olson DG, Lanahan AA, Tian L, Murphy SJ, Lo J, Lynd LR.	Biotechnol Biofuels	10.1186/s13068-015-0304-1	2015
Enzymology	Carbonic anhydrase in Acetobacterium woodii and other acetogenic bacteria.	Braus-Stromeyer SA, Schnappauf G, Braus GH, Gossner AS, Drake HL.	J Bacteriol	10.1128/jb.179.22.7197-7200.1997	1997
Metabolism	Genomic Analysis of Calderihabitans maritimus KKC1, a Thermophilic, Hydrogenogenic, Carboxydotrophic Bacterium Isolated from Marine Sediment.	Omae K, Yoneda Y, Fukuyama Y, Yoshida T, Sako Y.	Appl Environ Microbiol	10.1128/aem.00832-17	2017
	S-Layer proteins.	Sara M, Sleytr UB.	J Bacteriol	10.1128/jb.182.4.859-868.2000	2000
	The s-layer glycome-adding to the sugar coat of bacteria.	Ristl R, Steiner K, Zarschler K, Zayni S, Messner P, Schaffer C.	Int J Microbiol	10.1155/2011/127870	2011
	Domain structure of the Acetogenium kivui surface layer revealed by electron crystallography and sequence analysis.	Lupas A, Engelhardt H, Peters J, Santarius U, Volker S, Baumeister W.	J Bacteriol	10.1128/jb.176.5.1224-1233.1994	1994
	S-layer nanoglycobiology of bacteria.	Messner P, Steiner K, Zarschler K, Schaffer C.	Carbohydr Res	10.1016/j.carres.2007.12.025	2008
Enzymology	S-layer protein gene of Acetogenium kivui: cloning and expression in Escherichia coli and determination of the nucleotide sequence.	Peters J, Peters M, Lottspeich F, Baumeister W.	J Bacteriol	10.1128/jb.171.11.6307-6315.1989	1989
	Effects of small heat shock proteins from thermotolerant bacteria on the stress resistance of Escherichia coli to temperature, pH, and hyperosmolarity.	Sato Y, Okano K, Honda K.	Extremophiles	10.1007/s00792-023-01326-y	2024
Metabolism	Differential effects of sodium on hydrogen- and glucose-dependent growth of the acetogenic bacterium Acetogenium kivui.	Yang HC, Drake HL.	Appl Environ Microbiol	10.1128/aem.56.1.81-86.1990	1990
Metabolism	Characterization of the H2- and CO-dependent chemolithotrophic potentials of the acetogens Clostridium thermoaceticum and Acetogenium kivui.	Daniel SL, Hsu T, Dean SI, Drake HL	J Bacteriol	10.1128/jb.172.8.4464-4471.1990	1990
Metabolism	Nickel transport by the thermophilic acetogen Acetogenium kivui.	Yang HC, Daniel SL, Hsu TD, Drake HL	Appl Environ Microbiol	10.1128/aem.55.5.1078-1081.1989	1989
Phylogeny	Thermoanaerobacter uzonensis sp. nov., an anaerobic thermophilic bacterium isolated from a hot spring within the Uzon Caldera, Kamchatka, Far East Russia.	Wagner ID, Zhao W, Zhang CL, Romanek CS, Rohde M, Wiegel J	Int J Syst Evol Microbiol	10.1099/ijs.0.65343-0	2008

References

#676	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2030
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thermoanaerobacter kivui (DSM 2030, ATCC 33488, LKT-1)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000763575

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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