Name: Moorella thermoacetica Fontaine
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 16811

Type strain:

Species: Moorella thermoacetica

Strain Designation: Fontaine

Variant: Isotype of BacDive ID 16812

Culture col. no.: DSM 521, ATCC 35608, VPI 12954, JCM 9319, KCTC 5000, VPI 12984, CIP 108294

Strain history: CIP <- 2004, CCUG <- J.R. Andreesen <- L.G. Ljungdahl <- H.G. Wood <- H.A. Barker <- F. Fontaine

NCBI tax ID(s): 1525 (species)

SI-ID 41191

ATCC 35608, DSM 521, DSM 2955, JCM 9319, KCTC 3247, KCTC 5000, BCRC 14423, CCRC 14423, NRRL B-23504, CIP 108294

Special Collections

Other strains from Moorella thermoacetica

Section

Moorella thermoacetica Fontaine is an anaerobe, thermophilic, Gram-positive bacterium that was isolated from horse faeces.

Gram-positive
rod-shaped
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Moorellales*
	Family	*Moorellaceae*
	Genus	*Moorella*
	Species	**Moorella thermoacetica**
	Full Scientific Name (LPSN)	Moorella thermoacetica (Fontaine et al. 1942) Collins et al. 1994

	Synonym	Moorella thermoautotrophica
	Synonym	Clostridium thermoaceticum
	Synonym	Clostridium thermautotrophicum
	Synonym	Clostridium thermoautotrophicum
	Synonym	Clostridium thermaceticum

Information on morphological and physiological properties Morphology

[Ref.: #123500]	Gram stain	positive

[Ref.: #123500]	Cell shape	rod-shaped

[Ref.: #123500]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #265]

Culture medium

MOORELLA MEDIUM (DSMZ Medium 60)

[Ref.: #265]

Culture medium growth

[Ref.: #265]

Culture medium link

Medium recipe at MediaDive

[Ref.: #265]

Culture medium composition

expand / minimize

[Ref.: #32808]

Culture medium

MEDIUM 224 - for anaerobic bacteria TGV

[Ref.: #32808]

Culture medium growth

[Ref.: #32808]

Culture medium composition

expand / minimize

[Ref.: #123500]

Culture medium

CIP Medium 20

[Ref.: #123500]

Culture medium growth

[Ref.: #123500]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #265]	growth	55 °C
[Ref.: #32808]	growth	55 °C
[Ref.: #67770]	growth	60 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #265]	Oxygen tolerance	anaerobe
[Ref.: #123500]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %91.873

[Ref.: #265]	Name of produced compound	acetate kinase
[Ref.: #265]	Name of produced compound	formate dehydrogenase
[Ref.: #265]	Name of produced compound	formyltetrahydrofolate synthase

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #123500]	cellobiose ChEBI	-	degradation
[Ref.: #123500]	D-arabinose ChEBI	+	degradation
[Ref.: #123500]	D-fructose ChEBI	+	degradation
[Ref.: #123500]	D-glucose ChEBI	+	degradation
[Ref.: #123500]	D-xylose ChEBI	+	degradation
[Ref.: #123500]	esculin ChEBI	-	hydrolysis
[Ref.: #123500]	lactose ChEBI	-	degradation
[Ref.: #123500]	maltose ChEBI	-	degradation
[Ref.: #123500]	nitrate ChEBI	+	reduction
[Ref.: #123500]	nitrate ChEBI	+	respiration
[Ref.: #123500]	nitrite ChEBI	-	reduction
[Ref.: #123500]	salicin ChEBI	-	degradation
[Ref.: #123500]	sucrose ChEBI	-	degradation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #123500]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #67770]	acetate kinase		2.7.2.1
[Ref.: #123500]	beta-galactosidase	-	3.2.1.23
[Ref.: #123500]	catalase	-	1.11.1.6
[Ref.: #123500]	gelatinase	-
[Ref.: #123500]	oxidase	-
[Ref.: #123500]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #265]	Sample type/isolated from	horse faeces

[Ref.: #67770]	Sample type/isolated from	Horse feces

[Ref.: #123500]	Sample type/isolated from	Animal, Horse faeces
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Equidae (Horse)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB572912 (>99% sequence identity) for Moorella thermoacetica from Microbeatlas

Aquatic Samples	257
Soil Samples	87
Animal Samples	219
Plant Samples	32
Total Samples	595

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #265]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123500]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #265]	Moorella thermoacetica strain DSM 521 16S ribosomal RNA gene, partial sequence	AY656675	1430	GenBank ENA	1525 tax ID
[Ref.: #20218]	Moorella thermoacetica strain DSM 521 16S-23S ribosomal RNA intergenic spacer and 23S ribosomal RNA gene, partial sequence; and tRNA-Ile and tRNA-Ala genes, complete sequence	DQ192547	522	GenBank ENA	1525 tax ID	*
[Ref.: #20218]	Moorella thermoacetica gene for 16S ribosomal RNA, partial sequence, strain: JCM 9319	AB572912	1529	GenBank ENA	1525 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Moorella thermoacetica ATCC 35608	GCA_008121885	contig	GenBank ENA	1525 tax ID
[Ref.: #66792]	Moorella thermoacetica DSM 521	GCA_001267405	complete	GenBank ENA	1525 tax ID	*
[Ref.: #66792]	Moorella thermoacetica strain ATCC 35608	1525.21	wgs	PATRIC	1525 tax ID	*
[Ref.: #66792]	Moorella thermoacetica strain DSM 521	1525.3	complete	PATRIC	1525 tax ID	*
[Ref.: #66792]	Moorella thermoacetica DSM 521	2834890540	complete	JGI IMG/M	1525 tax ID	*
[Ref.: #66792]	Moorella thermoacetica DSM 521	2627853541	complete	JGI IMG/M	1525 tax ID	*

[Ref.: #265]

GC-content

56.0 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Moorella thermoacetica DSM 521 NCBI: GCA_001267405.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	54.165	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	91.873	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	93.088	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	75.16	no
[Ref.: #69480]	thermophilic	thermophileⓘ	yes	78.859	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	67.833	no

Availability in culture collections External links

[Ref.: #265]

Culture collection no.

DSM 521, ATCC 35608, VPI 12954, JCM 9319, KCTC 5000, VPI 12984, CIP 108294

[Ref.: #85824]

SI-ID 41191

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Old acetogens, new light.	Drake HL, Gossner AS, Daniel SL	Ann N Y Acad Sci	10.1196/annals.1419.016	2008	*
Genetics	Complete Genome Sequence of the Type Strain of the Acetogenic Bacterium Moorella thermoacetica DSM 521T.	Poehlein A, Bengelsdorf FR, Esser C, Schiel-Bengelsdorf B, Daniel R, Durre P	Genome Announc	10.1128/genomeA.01159-15	2015	*
Metabolism	Atypical one-carbon metabolism of an acetogenic and hydrogenogenic Moorella thermoacetica strain.	Jiang B, Henstra AM, Paulo PL, Balk M, van Doesburg W, Stams AJ	Arch Microbiol	10.1007/s00203-008-0435-x	2008	*

References

#265	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 521

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#32808 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#85824

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID41191.1 )

#123500 Collection of Institut Pasteur ; Curators of the CIP; CIP 108294
* These data were automatically processed and therefore are not curated

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Moorella thermoacetica DSM 521

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