Carboxydothermus ferrireducens (DSM 11255, VKM B-2392, JW/AS-Y7)

Name: Carboxydothermus ferrireducens (DSM 11255, VKM B-2392, JW/AS-Y7)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16807

Type strain

Strain Designation JW/AS-Y7

Culture col. no. DSM 11255 VKM B-2392 JW/AS-Y7

NCBI tax ID(s) 1119529 54265

Special Collections DSMZ

History <- J. Wiegel, University of Georgia, Department of Microbiology, Athens, USA; JW/AS-Y7 <- A. Slobodkin {1996}

Links

Carboxydothermus ferrireducens JW/AS-Y7 is an anaerobe bacterium that was isolated from mixed sample of sediment, water and biomass of hot spring.

anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 49059

DSM 11255,VKM B-2392

@ref 20215

Last LPSN update 2026-02-09 11:22:03

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Thermoanaerobacterales

Family Thermoanaerobacteraceae

Genus Carboxydothermus

Species Carboxydothermus ferrireducens

Full scientific name Carboxydothermus ferrireducens (Slobodkin et al. 1997) Slobodkin et al. 2006

Synonyms (2)

"Carboxydothermus hydrogenoformans subsp. ferrireducens"
Thermoterrabacterium ferrireducens

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
4262	THERMOTERRABACTERIUM MEDIUM (DSMZ Medium 778)		Medium recipe at MediaDive	Name: THERMOTERRABACTERIUM MEDIUM (DSMZ Medium 778) Composition: Na2-9,10-anthraquinone-2,6-disulfonate 8.2008 g/l NaHCO3 2.48509 g/l Yeast extract 0.994036 g/l KH2PO4 0.328032 g/l CaCl2 x 2 H2O 0.328032 g/l MgCl2 x 6 H2O 0.328032 g/l KCl 0.328032 g/l NH4Cl 0.328032 g/l HCl 0.00248509 g/l FeCl2 x 4 H2O 0.00149105 g/l NaOH 0.000497018 g/l NiCl2 x 6 H2O 0.000198807 g/l CoCl2 x 6 H2O 0.000188867 g/l MnCl2 x 4 H2O 9.94036e-05 g/l Pyridoxine hydrochloride 9.94036e-05 g/l ZnCl2 6.95825e-05 g/l (DL)-alpha-Lipoic acid 4.97018e-05 g/l p-Aminobenzoic acid 4.97018e-05 g/l Calcium D-(+)-pantothenate 4.97018e-05 g/l Nicotinic acid 4.97018e-05 g/l Riboflavin 4.97018e-05 g/l Thiamine HCl 4.97018e-05 g/l Na2MoO4 x 2 H2O 3.57853e-05 g/l Folic acid 1.98807e-05 g/l Biotin 1.98807e-05 g/l H3BO3 5.96421e-06 g/l Na2WO4 x 2 H2O 3.97614e-06 g/l Na2SeO3 x 5 H2O 2.98211e-06 g/l CuCl2 x 2 H2O 1.98807e-06 g/l Vitamin B12 9.94036e-07 g/l Glycerol Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
4262	positive	growth	65

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
4262	anaerobe
125438	anaerobe	91.836
125439	anaerobe	99.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
4262	mixed sample of sediment, water and biomass of hot spring	Yellowstone National Park, Calcite Spring area	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence LT547827 (>99% sequence identity) for Carboxydothermus from Microbeatlas

Aquatic Samples	17
Soil Samples	3
Animal Samples	16
Plant Samples	1
Total Samples	37

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4262	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1340977v1 assembly for Carboxydothermus ferrireducens DSM 11255	contig	GCA_013409775	1119529.9	2844895905	1119529	75.5
66792	ASM42756v1 assembly for Carboxydothermus ferrireducens DSM 11255	scaffold	GCA_000427565	1119529.5	2510065088	1119529	67.51

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Carboxydothermus ferrireducens strain JW/AS-Y7 16S small subunit ribosomal RNA gene, partial sequence	U76363	1503	54265
4262	Carboxydothermus ferrireducens partial 16S rRNA gene, strain DSM 11255T, clone 4	LT547827	1533	54265
4262	Carboxydothermus ferrireducens partial 16S rRNA gene, strain DSM 11255T, clone 3	LT547826	1534	54265
4262	Carboxydothermus ferrireducens partial 16S rRNA gene, strain DSM 11255T, clone 2	LT547825	1562	54265
4262	Carboxydothermus ferrireducens partial 16S rRNA gene, strain DSM 11255T, clone 1	LT547824	1562	54265

GC content

@ref	GC-content (mol%)	Method
4262	41.9	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Carboxydothermus ferrireducens DSM 11255
NCBI: GCA_000427565

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.10	no
125439	gram_stain	BacteriaNetⓘ	variable	85.20	no
125439	motility	BacteriaNetⓘ	yes	77.90	no
125439	spore_formation	BacteriaNetⓘ	yes	84.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Carboxydothermus ferrireducens DSM 11255 DSM 11255
NCBI: GCA_000427565.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	55.85	no
125438	anaerobic	anaerobicⓘ	yes	91.84	yes
125438	aerobic	aerobicⓘ	no	94.12	no
125438	spore-forming	spore-formingⓘ	yes	73.50	no
125438	thermophilic	thermophileⓘ	yes	78.45	yes
125438	flagellated	motile2+ⓘ	yes	71.23	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	A survey of the Desulfuromonadia "cytochromome" provides a glimpse of the unexplored diversity of multiheme cytochromes in nature.	Soares R, Fonseca BM, Nash BW, Paquete CM, Louro RO.	BMC Genomics	10.1186/s12864-024-10872-4	2024
	Identification of novel tail-anchored membrane proteins integrated by the bacterial twin-arginine translocase.	Gallego-Parrilla JJ, Severi E, Chandra G, Palmer T.	Microbiology (Reading)	10.1099/mic.0.001431	2024
Pathogenicity	Discovery of the Alternaria mycotoxins alterperylenol and altertoxin I as novel immunosuppressive and antiestrogenic compounds in vitro.	Crudo F, Partsch V, Braga D, Blazevic R, Rollinger JM, Varga E, Marko D.	Arch Toxicol	10.1007/s00204-024-03877-1	2025
Metabolism	The Sporomusa type Nfn is a novel type of electron-bifurcating transhydrogenase that links the redox pools in acetogenic bacteria.	Kremp F, Roth J, Muller V.	Sci Rep	10.1038/s41598-020-71038-2	2020
Metabolism	Insight into Energy Conservation via Alternative Carbon Monoxide Metabolism in Carboxydothermus pertinax Revealed by Comparative Genome Analysis.	Fukuyama Y, Omae K, Yoneda Y, Yoshida T, Sako Y.	Appl Environ Microbiol	10.1128/aem.00458-18	2018
	Actinomycins with altered threonine units in the beta-peptidolactone.	Bitzer J, Gesheva V, Zeeck A.	J Nat Prod	10.1021/np060063g	2006
Metabolism	Genomic Analysis of Calderihabitans maritimus KKC1, a Thermophilic, Hydrogenogenic, Carboxydotrophic Bacterium Isolated from Marine Sediment.	Omae K, Yoneda Y, Fukuyama Y, Yoshida T, Sako Y.	Appl Environ Microbiol	10.1128/aem.00832-17	2017
Pathogenicity	Microfiltration results in the loss of analytes and affects the in vitro genotoxicity of a complex mixture of Alternaria toxins.	Aichinger G, Zivna N, Varga E, Crudo F, Warth B, Marko D.	Mycotoxin Res	10.1007/s12550-020-00405-9	2020
Phylogeny	Phenotypic and Molecular-Phylogenetic Analyses Reveal Distinct Features of Crown Gall-Associated Xanthomonas Strains.	Mafakheri H, Taghavi SM, Zarei S, Rahimi T, Hasannezhad MS, Portier P, Fischer-Le Saux M, Dimkic I, Koebnik R, Kuzmanovic N, Osdaghi E.	Microbiol Spectr	10.1128/spectrum.00577-21	2022
Phylogeny	Investigating the Phylogenetic Range of Gibberellin Biosynthesis in Bacteria.	Nagel R, Peters RJ.	Mol Plant Microbe Interact	10.1094/mpmi-01-17-0001-r	2017
Metabolism	Dual effectiveness of Alternaria but not Fusarium mycotoxins against human topoisomerase II and bacterial gyrase.	Jarolim K, Del Favero G, Ellmer D, Stark TD, Hofmann T, Sulyok M, Humpf HU, Marko D.	Arch Toxicol	10.1007/s00204-016-1855-z	2017
Metabolism	Using gas mixtures of CO, CO₂ and H₂ as microbial substrates: the do's and don'ts of successful technology transfer from laboratory to production scale.	Takors R, Kopf M, Mampel J, Bluemke W, Blombach B, Eikmanns B, Bengelsdorf FR, Weuster-Botz D, Durre P.	Microb Biotechnol	10.1111/1751-7915.13270	2018
Metabolism	Immobilization of arsenite and ferric iron by Acidithiobacillus ferrooxidans and its relevance to acid mine drainage.	Duquesne K, Lebrun S, Casiot C, Bruneel O, Personne JC, Leblanc M, Elbaz-Poulichet F, Morin G, Bonnefoy V.	Appl Environ Microbiol	10.1128/aem.69.10.6165-6173.2003	2003
Enzymology	Extracellular Fe(III) reductase structure reveals a modular organization enabling S-layer insertion and electron transfer to insoluble substrates.	Tikhonova TV, Osipov EM, Dergousova NI, Boyko KM, Elizarov IM, Gavrilov SN, Khrenova MG, Robb FT, Solovieva AY, Bonch-Osmolovskaya EA, Popov VO.	Structure	10.1016/j.str.2022.12.010	2023
	Moving towards the enhancement of extracellular electron transfer in electrogens	Verma M, Singh V, Mishra V.	World J Microbiol Biotechnol.		2023
	Moving towards the enhancement of extracellular electron transfer in electrogens.	Verma M, Singh V, Mishra V.	World J Microbiol Biotechnol	10.1007/s11274-023-03582-8	2023
	Electron transfer in multicentre redox proteins: from fundamentals to extracellular electron transfer.	Bayar B, Soares R, Nalakath H, Alves A, Paquete CM, Louro RO.	Biosci Rep	10.1042/bsr20240576	2025
	Anaerobic corrosion of steel wire by Geoalkalibacter ferrihydriticus under alkaline autotrophic conditions.	Zavarzina DG, Chistyakova NI, Kohout J, Merkel AY, Perevalova AA, Kubaniova D, Chernov MS, Frolov EN, Klyuev AL, Gavrilov SN.	Appl Environ Microbiol	10.1128/aem.01848-24	2025
Metabolism	Nonelectroactive clostridium obtains extracellular electron transfer-capability after forming chimera with Geobacter.	Liu X, Ye Y, Yang N, Cheng C, Rensing C, Jin C, Nealson KH, Zhou S.	ISME Commun	10.1093/ismeco/ycae058	2024
	A New Paradigm of Multiheme Cytochrome Evolution by Grafting and Pruning Protein Modules.	Soares R, Costa NL, Paquete CM, Andreini C, Louro RO.	Mol Biol Evol	10.1093/molbev/msac139	2022
Genetics	Genomic Insights into Syntrophic Lifestyle of 'Candidatus Contubernalis alkaliaceticus' Based on the Reversed Wood-Ljungdahl Pathway and Mechanism of Direct Electron Transfer.	Frolov EN, Gavrilov SN, Toshchakov SV, Zavarzina DG.	Life (Basel)	10.3390/life13102084	2023
Enzymology	Characterization of a soluble oxidoreductase from the thermophilic bacterium Carboxydothermus ferrireducens.	Onyenwoke RU, Geyer R, Wiegel J.	Extremophiles	10.1007/s00792-009-0255-1	2009
	Novel Extracellular Electron Transfer Channels in a Gram-Positive Thermophilic Bacterium.	Gavrilov SN, Zavarzina DG, Elizarov IM, Tikhonova TV, Dergousova NI, Popov VO, Lloyd JR, Knight D, El-Naggar MY, Pirbadian S, Leung KM, Robb FT, Zakhartsev MV, Bretschger O, Bonch-Osmolovskaya EA.	Front Microbiol	10.3389/fmicb.2020.597818	2020
Metabolism	Genomic Insights into the Carbon and Energy Metabolism of a Thermophilic Deep-Sea Bacterium Deferribacter autotrophicus Revealed New Metabolic Traits in the Phylum Deferribacteres.	Slobodkin A, Slobodkina G, Allioux M, Alain K, Jebbar M, Shadrin V, Kublanov I, Toshchakov S, Bonch-Osmolovskaya E.	Genes (Basel)	10.3390/genes10110849	2019
	Iron or sulfur respiration-an adaptive choice determining the fitness of a natronophilic bacterium Dethiobacter alkaliphilus in geochemically contrasting environments.	Zavarzina DG, Merkel AY, Klyukina AA, Elizarov IM, Pikhtereva VA, Rusakov VS, Chistyakova NI, Ziganshin RH, Maslov AA, Gavrilov SN.	Front Microbiol	10.3389/fmicb.2023.1108245	2023
	The first cultivated representatives of the actinobacterial lineage OPB41 isolated from subsurface environments constitute a novel order Anaerosomatales.	Khomyakova MA, Zavarzina DG, Merkel AY, Klyukina AA, Pikhtereva VA, Gavrilov SN, Slobodkin AI.	Front Microbiol	10.3389/fmicb.2022.1047580	2022
Enzymology	Spatial distribution of an uranium-respiring betaproteobacterium at the Rifle, CO field research site.	Koribanics NM, Tuorto SJ, Lopez-Chiaffarelli N, McGuinness LR, Haggblom MM, Williams KH, Long PE, Kerkhof LJ.	PLoS One	10.1371/journal.pone.0123378	2015
Metabolism	The Geoglobus acetivorans genome: Fe(III) reduction, acetate utilization, autotrophic growth, and degradation of aromatic compounds in a hyperthermophilic archaeon.	Mardanov AV, Slododkina GB, Slobodkin AI, Beletsky AV, Gavrilov SN, Kublanov IV, Bonch-Osmolovskaya EA, Skryabin KG, Ravin NV.	Appl Environ Microbiol	10.1128/aem.02705-14	2015
Metabolism	Extracellular electron transfer to Fe(III) oxides by the hyperthermophilic archaeon Geoglobus ahangari via a direct contact mechanism.	Manzella MP, Reguera G, Kashefi K.	Appl Environ Microbiol	10.1128/aem.01566-13	2013
Metabolism	Evidence for direct electron transfer by a gram-positive bacterium isolated from a microbial fuel cell.	Wrighton KC, Thrash JC, Melnyk RA, Bigi JP, Byrne-Bailey KG, Remis JP, Schichnes D, Auer M, Chang CJ, Coates JD.	Appl Environ Microbiol	10.1128/aem.05365-11	2011
Enzymology	Regulation of multiple carbon monoxide consumption pathways in anaerobic bacteria.	Techtmann SM, Colman AS, Murphy MB, Schackwitz WS, Goodwin LA, Robb FT.	Front Microbiol	10.3389/fmicb.2011.00147	2011
	Fe-oxide grain coatings support bacterial Fe-reducing metabolisms in 1.7-2.0 km-deep subsurface quartz arenite sandstone reservoirs of the Illinois Basin (USA).	Dong Y, Sanford RA, Locke RA, Cann IK, Mackie RI, Fouke BW.	Front Microbiol	10.3389/fmicb.2014.00511	2014
	The complete genome sequence and emendation of the hyperthermophilic, obligate iron-reducing archaeon "Geoglobus ahangari" strain 234(T).	Manzella MP, Holmes DE, Rocheleau JM, Chung A, Reguera G, Kashefi K.	Stand Genomic Sci	10.1186/s40793-015-0035-8	2015
Phylogeny	Reclassification of Thermoterrabacterium ferrireducens as Carboxydothermus ferrireducens comb. nov., and emended description of the genus Carboxydothermus.	Slobodkin AI, Sokolova TG, Lysenko AM, Wiegel J	Int J Syst Evol Microbiol	10.1099/ijs.0.64503-0	2006
Phylogeny	Rise of Microbial Culturomics: Noncontiguous Finished Genome Sequence and Description of Beduini massiliensis gen. nov., sp. nov.	Mourembou G, Yasir M, Azhar EI, Lagier JC, Bibi F, Jiman-Fatani AA, Helmy N, Robert C, Rathored J, Fournier PE, Raoult D, Million M.	OMICS	10.1089/omi.2015.0143	2015
Phylogeny	Thermoterrabacterium ferrireducens gen. nov., sp. nov., a thermophilic anaerobic dissimilatory Fe(III)-reducing bacterium from a continental hot spring.	Slobodkin A, Reysenbach AL, Strutz N, Dreier M, Wiegel J	Int J Syst Bacteriol	10.1099/00207713-47-2-541	1997

References

#4262	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11255
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Carboxydothermus ferrireducens (DSM 11255, VKM B-2392, JW/AS-Y7)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_013409775

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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