Name: Tepidanaerobacter syntrophicus JL
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 16798

Type strain:

Species: Tepidanaerobacter syntrophicus

Strain Designation: JL

Culture col. no.: DSM 15584, JCM 12098, NBRC 100060

Strain history: Y. Sekiguchi strain JL.

NCBI tax ID(s): 224999 (species)

Other strains from Tepidanaerobacter syntrophicus

T. syntrophicus JE, DSM 15585, JCM 12099, NBRC ...
T. syntrophicus OL, DSM 15586, JCM 12100, NBRC ...

Section

Tepidanaerobacter syntrophicus JL is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from thermophilic anaerobic digested sewage sludge.

Gram-negative
rod-shaped
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Thermosediminibacterales*
	Family	*Tepidanaerobacteraceae*
	Genus	*Tepidanaerobacter*
	Species	**Tepidanaerobacter syntrophicus**
	Full Scientific Name (PNU)	Tepidanaerobacter syntrophicus Sekiguchi et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31717]	Gram stain	negative
[Ref.: #31717]	Cell length	1.5-10 µm
[Ref.: #31717]	Cell width	0.6-0.8 µm
[Ref.: #31717]	Cell shape	rod-shaped
[Ref.: #31717]	Motility	no

[Ref.: #31717]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5906]

Culture medium

TEPIDANAEROBACTER MEDIUM (DSMZ Medium 1051)

[Ref.: #5906]

Culture medium growth

[Ref.: #5906]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #5906]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #5906]	growth	45 °C
[Ref.: #31717]	growth	25-60 °C
[Ref.: #31717]	optimum	45-50 °C
[Ref.: #67770]	growth	45 °C

[Ref.: #5906]	Temperature range	thermophilic
[Ref.: #31717]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

	pH	Kind of pH		pH	pH range
[Ref.: #31717]			optimum	7
[Ref.: #31717]			growth	5.5-8.5	alkaliphile

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5906]	Oxygen tolerance	anaerobe
[Ref.: #31717]	Oxygen tolerance	anaerobe

[Ref.: #31717]

Ability of spore formation

[Ref.: #31717]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31717]	arabinose ChEBI	+	carbon source
[Ref.: #31717]	galactose ChEBI	+	carbon source
[Ref.: #31717]	malate ChEBI	+	carbon source
[Ref.: #31717]	raffinose ChEBI	+	carbon source
[Ref.: #31717]	ribose ChEBI	+	carbon source

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	aminopropanol phosphate biosynthesis	100	2 of 2
[Ref.: #66794]	C4 and CAM-carbon fixation	100	8 of 8
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	isoleucine metabolism	100	8 of 8
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	gluconeogenesis	100	8 of 8
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	palmitate biosynthesis	90.91	20 of 22
[Ref.: #66794]	threonine metabolism	90	9 of 10
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	valine metabolism	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	88.89	8 of 9
[Ref.: #66794]	pyrimidine metabolism	80	36 of 45
[Ref.: #66794]	metabolism of amino sugars and derivatives	80	4 of 5
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	vitamin B12 metabolism	79.41	27 of 34
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	NAD metabolism	77.78	14 of 18
[Ref.: #66794]	serine metabolism	77.78	7 of 9
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	degradation of sugar alcohols	75	12 of 16
[Ref.: #66794]	purine metabolism	73.4	69 of 94
[Ref.: #66794]	histidine metabolism	72.41	21 of 29
[Ref.: #66794]	heme metabolism	71.43	10 of 14
[Ref.: #66794]	propanol degradation	71.43	5 of 7
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	Entner Doudoroff pathway	70	7 of 10
[Ref.: #66794]	propionate fermentation	70	7 of 10
[Ref.: #66794]	vitamin B1 metabolism	69.23	9 of 13
[Ref.: #66794]	alanine metabolism	68.97	20 of 29
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	glycolate and glyoxylate degradation	66.67	4 of 6
[Ref.: #66794]	flavin biosynthesis	66.67	10 of 15
[Ref.: #66794]	methane metabolism	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	d-mannose degradation	66.67	6 of 9
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	tetrahydrofolate metabolism	64.29	9 of 14
[Ref.: #66794]	d-xylose degradation	63.64	7 of 11
[Ref.: #66794]	metabolism of disaccharids	63.64	7 of 11
[Ref.: #66794]	tryptophan metabolism	63.16	24 of 38
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	urea cycle	61.54	8 of 13
[Ref.: #66794]	leucine metabolism	61.54	8 of 13
[Ref.: #66794]	cysteine metabolism	61.11	11 of 18
[Ref.: #66794]	glutamate and glutamine metabolism	60.71	17 of 28
[Ref.: #66794]	oxidative phosphorylation	60.44	55 of 91
[Ref.: #66794]	4-hydroxyphenylacetate degradation	60	6 of 10
[Ref.: #66794]	hydrogen production	60	3 of 5
[Ref.: #66794]	factor 420 biosynthesis	60	3 of 5
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
[Ref.: #66794]	ubiquinone biosynthesis	57.14	4 of 7
[Ref.: #66794]	reductive acetyl coenzyme A pathway	57.14	4 of 7
[Ref.: #66794]	cardiolipin biosynthesis	57.14	4 of 7
[Ref.: #66794]	degradation of pentoses	57.14	16 of 28
[Ref.: #66794]	pentose phosphate pathway	54.55	6 of 11
[Ref.: #66794]	proline metabolism	54.55	6 of 11
[Ref.: #66794]	methionine metabolism	53.85	14 of 26
[Ref.: #66794]	CMP-KDO biosynthesis	50	2 of 4
[Ref.: #66794]	citric acid cycle	50	7 of 14
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	myo-inositol biosynthesis	50	5 of 10
[Ref.: #66794]	dTDPLrhamnose biosynthesis	50	4 of 8
[Ref.: #66794]	kanosamine biosynthesis II	50	1 of 2
[Ref.: #66794]	lysine metabolism	50	21 of 42
[Ref.: #66794]	biotin biosynthesis	50	2 of 4
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	lactate fermentation	50	2 of 4
[Ref.: #66794]	toluene degradation	50	2 of 4
[Ref.: #66794]	ketogluconate metabolism	50	4 of 8
[Ref.: #66794]	isoprenoid biosynthesis	50	13 of 26
[Ref.: #66794]	glycogen biosynthesis	50	2 of 4
[Ref.: #66794]	starch degradation	50	5 of 10
[Ref.: #66794]	degradation of sugar acids	48	12 of 25
[Ref.: #66794]	arginine metabolism	45.83	11 of 24
[Ref.: #66794]	non-pathway related	44.74	17 of 38
[Ref.: #66794]	molybdenum cofactor biosynthesis	44.44	4 of 9
[Ref.: #66794]	glutathione metabolism	42.86	6 of 14
[Ref.: #66794]	lipoate biosynthesis	40	2 of 5
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	glycogen metabolism	40	2 of 5
[Ref.: #66794]	polyamine pathway	39.13	9 of 23
[Ref.: #66794]	ascorbate metabolism	36.36	8 of 22
[Ref.: #66794]	vitamin B6 metabolism	36.36	4 of 11
[Ref.: #66794]	lipid metabolism	35.48	11 of 31
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	degradation of hexoses	33.33	6 of 18
[Ref.: #66794]	sulfoquinovose degradation	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	sulfate reduction	30.77	4 of 13
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	tyrosine metabolism	28.57	4 of 14
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	phenol degradation	25	5 of 20
[Ref.: #66794]	carnitine metabolism	25	2 of 8
[Ref.: #66794]	phenylpropanoid biosynthesis	23.08	3 of 13
[Ref.: #66794]	allantoin degradation	22.22	2 of 9
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5906]	Sample type/isolated from	thermophilic anaerobic digested sewage sludge
[Ref.: #5906]	Geographic location (country and/or sea, region)	Niigata
[Ref.: #5906]	Country	Japan
[Ref.: #5906]	Country ISO 3 Code	JPN
[Ref.: #5906]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Sludge from a thermophilic digester decomposing municipal solid waste
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Waste	#Sewage sludge
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB106353 (>99% sequence identity) for Tepidanaerobacter syntrophicus subclade from Microbeatlas

Aquatic Samples	274
Soil Samples	31
Animal Samples	60
Plant Samples	52
Total Samples	417

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5906]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5906]	Tepidanaerobacter syntrophicus gene for 16S rRNA, partial sequence, strain: JL	AB106353	1451	ENA	224999 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Tepidanaerobacter syntrophicus strain JL	224999.3	wgs	PATRIC	224999 tax ID	*
[Ref.: #66792]	Tepidanaerobacter syntrophicus JL	2814123063	complete	JGI IMG/M	224999 tax ID	*
[Ref.: #67770]	Tepidanaerobacter syntrophicus JL	GCA_001485475	scaffold	GenBank	224999 tax ID

[Ref.: #31717]	GC-content	38 mol%
[Ref.: #67770]	GC-content	38.6 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	38 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #5906]

Culture collection no.

DSM 15584, JCM 12098, NBRC 100060

[Ref.: #20218]

Associated Passport(s) in StrainInfo

690505, 690506, 690507

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Tepidanaerobacter syntrophicus gen. nov., sp. nov., an anaerobic, moderately thermophilic, syntrophic alcohol- and lactate-degrading bacterium isolated from thermophilic digested sludges.	Sekiguchi Y, Imachi H, Susilorukmi A, Muramatsu M, Ohashi A, Harada H, Hanada S, Kamagata Y	Int J Syst Evol Microbiol	10.1099/ijs.0.64112-0	2006	*
Phylogeny	Description of Biomaibacter acetigenes gen. nov., sp. nov., and proposal of Thermosediminibacterales ord. nov. containing two novel families of Tepidanaerobacteraceae fam. nov. and Thermosediminibacteraceae fam. nov.	Zhang X, Tu B, Lv XM, Lawson PA, Yang M, Dai LR, Zhang H, Tang YQ, Deng Y, Cheng L	Int J Syst Evol Microbiol	10.1099/ijsem.0.003701	2019	*

References

#5906

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15584

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31717

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27993 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#27993

IJSEM 1629 2006 ( DOI 10.1099/ijs.0.64112-0 , PubMed 16825639 )

* These data were automatically processed and therefore are not curated

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