Caldanaerobacter subterraneus subsp. tengcongensis (DSM 15242, CGMCC AS 1.2430, JCM 11007)

Name: Caldanaerobacter subterraneus subsp. tengcongensis (DSM 15242, CGMCC AS 1.2430, JCM 11007)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16796

Type strain

Strain Designation MB4

Culture col. no. DSM 15242 CGMCC AS 1.2430 JCM 11007 MB4 NBRC 100824 1 more

NCBI tax ID(s) 119072

Special Collections DSMZ JCM

History <- JCM; MB4 Y. Ma MB4.

Links

Caldanaerobacter subterraneus subsp. tengcongensis MB4 is an anaerobe bacterium that was isolated from mixed water and sediment sample.

anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 101000

JCM 11007,DSM 15242,AS 1.2430,NBRC 100824,CGMCC 1.2430

@ref 20215

Last LPSN update 2026-02-09 11:22:03

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Thermoanaerobacterales

Family Thermoanaerobacteraceae

Genus Caldanaerobacter

Species Caldanaerobacter subterraneus subsp. tengcongensis

Full scientific name Caldanaerobacter subterraneus subsp. tengcongensis (Xue et al. 2001) Fardeau et al. 2004

Synonyms (1)

Thermoanaerobacter tengcongensis

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
5778	THERMOANAEROBACTER KREATINOPHILUS MEDIUM (DSMZ Medium 965)		Medium recipe at MediaDive	Name: THERMOANAEROBACTER KREATINOPHILUS MEDIUM (DSMZ Medium 965) Composition: Starch 9.90099 g/l Na2S2O3 x 5 H2O 4.9505 g/l Tryptone 1.9802 g/l NH4Cl 0.990099 g/l Yeast extract 0.990099 g/l MgCl2 x 6 H2O 0.49505 g/l L-Cysteine HCl x H2O 0.49505 g/l KH2PO4 0.29703 g/l K2HPO4 0.29703 g/l KCl 0.19802 g/l MgSO4 x 7 H2O 0.029703 g/l Nitrilotriacetic acid 0.0148515 g/l NaCl 0.00990099 g/l MnSO4 x H2O 0.00495049 g/l CoSO4 x 7 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.00178218 g/l CaCl2 x 2 H2O 0.000990099 g/l FeSO4 x 7 H2O 0.000990099 g/l Sodium resazurin 0.00049505 g/l NiCl2 x 6 H2O 0.00029703 g/l AlK(SO4)2 x 12 H2O 0.00019802 g/l Na2MoO4 x 2 H2O 9.90099e-05 g/l CuSO4 x 5 H2O 9.90099e-05 g/l H3BO3 9.90099e-05 g/l Na2WO4 x 2 H2O 3.9604e-06 g/l Na2SeO3 x 5 H2O 2.9703e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
5778	positive	growth	75
67770	positive	growth	75

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
5778	anaerobe
125438	anaerobe	94.823
125439	anaerobe	99.9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
5778	mixed water and sediment sample	Yunnan Province, Tengcong, hot spring	China	CHN	Asia
67770	Hot spring	Tengcong	China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AF209708 (>99% sequence identity) for Caldanaerobacter from Microbeatlas

Aquatic Samples	2
Soil Samples
Animal Samples	1
Plant Samples
Total Samples	3

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5778	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM708v1 assembly for Caldanaerobacter subterraneus subsp. tengcongensis MB4	complete	GCA_000007085	273068.5	637000318	273068	99.16
66792	ASM2480713v1 assembly for Caldanaerobacter subterraneus subsp. tengcongensis MB4	contig	GCA_024807135	273068.17	2927245718	273068	79

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5778	Thermoanaerobacter tengcongensis 16S ribosomal RNA gene, partial sequence	AF209708	1526		119072
67770	Caldanaerobacter subterraneus subsp. tengcongensis gene for 16S ribosomal RNA, partial sequence	AB597259	1496		119072

GC content

@ref	GC-content (mol%)	Method
5778	37	sequence analysis
67770	33	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Caldanaerobacter subterraneus subsp. tengcongensis MB4
NCBI: GCA_024807135

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	67.60	no
125439	motility	BacteriaNetⓘ	yes	68.50	no
125439	gram_stain	BacteriaNetⓘ	variable	73.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Caldanaerobacter subterraneus subsp. tengcongensis MB4 MB4
NCBI: GCA_000007085.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	55.69	no
125438	anaerobic	anaerobicⓘ	yes	94.82	yes
125438	aerobic	aerobicⓘ	no	96.27	yes
125438	spore-forming	spore-formingⓘ	yes	67.86	no
125438	thermophilic	thermophileⓘ	yes	73.42	yes
125438	flagellated	motile2+ⓘ	yes	73.45	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Enhanced extracellular alpha-amylase production in Brevibacillus choshinensis by optimizing extracellular degradation and folding environment.	Yao D, Zhang K, Zhu X, Su L, Wu J.	J Ind Microbiol Biotechnol	10.1093/jimb/kuab061	2022
Metabolism	Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes.	Loder AJ, Zeldes BM, Garrison GD, Lipscomb GL, Adams MW, Kelly RM.	Appl Environ Microbiol	10.1128/aem.02028-15	2015
Enzymology	Thermostable lipases from the extreme thermophilic anaerobic bacteria Thermoanaerobacter thermohydrosulfuricus SOL1 and Caldanaerobacter subterraneus subsp. tengcongensis.	Royter M, Schmidt M, Elend C, Hobenreich H, Schafer T, Bornscheuer UT, Antranikian G.	Extremophiles	10.1007/s00792-009-0265-z	2009
Enzymology	Molecular cloning, heterologous expression, and enzymatic characterization of lysoplasmalogen-specific phospholipase D from Thermocrispum sp.	Matsumoto Y, Kashiwabara N, Oyama T, Murayama K, Matsumoto H, Sakasegawa SI, Sugimori D.	FEBS Open Bio	10.1002/2211-5463.12131	2016
	Fermentation of Mannitol Extracts From Brown Macro Algae by Thermophilic Clostridia.	Chades T, Scully SM, Ingvadottir EM, Orlygsson J.	Front Microbiol	10.3389/fmicb.2018.01931	2018
Phylogeny	Isolation from oil reservoirs of novel thermophilic anaerobes phylogenetically related to Thermoanaerobacter subterraneus: reassignment of T. subterraneus, Thermoanaerobacter yonseiensis, Thermoanaerobacter tengcongensis and Carboxydibrachium pacificum to Caldanaerobacter subterraneus gen. nov., sp. nov., comb. nov. as four novel subspecies.	Fardeau ML, Salinas MB, L'Haridon S, Jeanthon C, Verhe F, Cayol JL, Patel BKC, Garcia JL, Ollivier B	Int J Syst Evol Microbiol	10.1099/ijs.0.02711-0	2004
Phylogeny	Thermoanaerobacter tengcongensis sp. nov., a novel anaerobic, saccharolytic, thermophilic bacterium isolated from a hot spring in Tengcong, China.	Xue Y, Xu Y, Liu Y, Ma Y, Zhou P	Int J Syst Evol Microbiol	10.1099/00207713-51-4-1335	2001

References

#5778	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15242
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Caldanaerobacter subterraneus subsp. tengcongensis (DSM 15242, CGMCC AS 1.2430, JCM 11007)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000007085

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media